• 보존과학연구
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• s.25
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• pp.31-45
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• 2004

The archeological remains usually exist in some excavated ancient soils. The ancients oils are buried in underground with the environmental, biological and social facts for circumstantial evidence at past times. Consequently, it is very important thing to carry out scientific analysis of the ancient soils side by side with archeological study. In this study, we accumulated some basic data for scientific analysis of 5 kinds of ancient soils excavated at Wanggung-ri, Iksan city. So we obtained some characterizations of organic chemical source, ancient parasite egg, and some seeds in the soils. The organic sources showed the content of high organic material as 7~22%, and strong acidic condition as pH 2~6. It is indicated that Wanggung-ri soils have included so many organic materials from the degradation of biological remains. Most of all, we searched a lot of eggs of parasite Trichuris trichiura and so it is possible that this area had been a ancient toilet at that times. The more scientific analysis of the soils will be showed us about the utility of the area, ancient dietary life style, ancient environment and ancient human diseases.

• Journal of The Geomorphological Association of Korea
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• v.19 no.1
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• pp.41-56
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• 2012

The purpose of this study is to look into the location environment of prehistoric and ancient remains (hereinafter referred as remains) in Daejeon Area. For the purpose of this, out of remains reported in investigation areas so far with the object of remains (total 22 places) of New Stone Age -Proto Three Kingdom period, geographical and Geomorphological analysis was conducted. The results are like the following. Most of the remains are distributed over hills especially flat surface - semi-gentle slope of hills. The bed rocks of the remains (21 places, about 95%) are composed of double-mica granite. The remains (19 places, 86%) retain 'the 1st-2nd small scale river and '5th or higher medium scale river'within comparatively short distance at the same time. The average altitude of remains is 71m and most remains are located over the altitude of 50 - 90m. The average gradient of hillslop where the remains are located is $6.7^{\circ}$ and the remains (15 places, 71%) are located over the gradient of about $0-15^{\circ}$. And the tendency that the higher the gradient of remains the smaller the number of remains was confirmed. From the above, it is judged that the inhabitants who lived in Daejeon Area at that time preferred hills with comparatively not high altitude and the earth's surface of low gradient and in addition they recognized water (river) as being important when they selected the location of settlement (or dwelling sites group).

• Animal Bioscience
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• v.35 no.8
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• pp.1141-1150
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• 2022

Objective: To understand the domestication and spread of horses in history, genetic information is essential. However, mitogenetic traits of ancient or medieval horses have yet to be comprehensively revealed, especially for East Asia. This study thus set out to reveal the maternal lineage of skeletal horse remains retrieved from a 15th century archaeological site (Gongpyeongdong) at Old Seoul City in South Korea. Methods: We extracted DNA from the femur of Equus caballus (SNU-A001) from Joseon period Gongpyeongdong site. Mitochondrial (mt) DNA (HRS 15128-16116) of E. caballus was amplified by polymerase chain reaction. Cloning and sequencing were conducted for the mtDNA amplicons. The sequencing results were analyzed by NCBI/BLAST and phylogenetic tool of MEGA7 software. Results: By means of mtDNA cytochrome b and D-loop analysis, we found that the 15th century Korean horse belonged to haplogroup Q representing those horses that have historically been raised widely in East Asia. Conclusion: The horse is unique among domesticated animals for the remarkable impact it has on human civilization in terms of transportation and trade. Utilizing the Joseon-period horse remains, we can obtain clues to reveal the genetic traits of Korean horse that existed before the introduction of Western horses.

• Korean Journal of Heritage: History & Science
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• v.41 no.1
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• pp.99-108
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• 2008

The paleogenetic analysis has become an increasingly important subject of archaeological, anthropological, biological as well as public interest. Recently, scientific research for human skeletal remains was more activated because of increasing awareness of the valuable archaeological information by the ancient DNA analysis. State of preservation of organic remains vary in different soil and burying environmental condition. Almost all available tissue disappear to analysis ancient DNA of bone in acidic soil caused by climate and geological features in Korea. Many preserved human remains excavated in the 'Heogwakmyo'(limelayered tomb of Chosun Dynasty Period) is able to explain through the relationship between burial conditions and bone survival form the burial method and ceremony. Ancient DNA analysis of excavated human bone form ancient tomb requires to remove contaminants such as microorganism's DNA and soil components that affect authentic results. Particularly, contamination control of contemporary human DNA is major serious problem and should verified by criteria of authenticity. In order to understand migration and culture of ancient population, when possible, ancient DNA studies needs to go abreast both radiocarbon and stable isotope studies because the dietary inferences will suggest ancient subsistence and settlement patterns. Also when the paleogenetic research supported with the arts and humanities research such as physical anthropology and archaeology, more valuable ancient genetic information is providing a unique results about evolutionary and population genetics studies to reconstruct the past.

• 보존과학연구
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• s.22
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• pp.27-40
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• 2001

DNA typing is often used to determine identity from human remains. Recently, the molecular biological analysis of ancient deposits has become possible since methods for the recovery of DNA conserved in bones or teeth from archaeological remains have been developed. In the field of archaeology, one of the most promising approaches is to identify the individuals present in a mass burial site. We performed nuclear DNA typing and mitochondrial DNA sequencing analysis based on PCR from a Korea ancient human remain excavated from Sa-chon Nuk-island and civilian access controlline(CACL). A femur bone were collected and successfully subjected to DNA extraction, quantification, PCR amplification, and subsequently typed for several shot tandem repeat(STR)loci. 4 types of STR systems used in this study were CTT multiplex(CSF1PO, TPOX, TH01), FFv multiplex(F13A01, FESFPS, vWA), Silver STRⅢ multiplex(D16S539, D7S820, D13S317), and amelogenin for sex determination. This studies are primarily concerned with the extraction, amplification, and DNA typing of ancient human bone DNA samples. Also, it is suggestive of importance about closely relationship between both fields of archaeology and molecular biology.

• Journal of architectural history
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• v.26 no.2
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• pp.67-74
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• 2017

A very unique case is found in the architectural remains related to the north and south square-platform at the lecture hall site of Hwangnyongsa, that of Anyangsa, and the estimated lecture hall site of Beopsusa as they are not observed in the other lecture halls. The platform has been discovered at only those three Buddhist temples from Goryeo, and its case has not been found in ancient Buddhist temples of China and Japan. This study thus set out to investigate the north and south square-platform at lecture hall sites by examining in details its architectural remains at the three Buddhist temples and reviewing the Buddhist literature about the lecture halls of ancient Buddhist temples. Based on the findings, it was estimated that the architectural remains of north and south square-platform at the lecture hall sites of Buddhist temples were those of platform for Buddhist sermons or those of high chair platform. While the north square-platform involved the remains of north high chair for the Lecturer that gave lectures on the Buddhist scriptures, the south one did those of south high chair for the Reader who recited the Buddhist scriptures.

• 보존과학연구
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• s.29
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• pp.137-148
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• 2008

Analyses of ancient DNA (aDNA) from archaeological and historical skeletal material are characterized by low quality. Many soil contaminants such as humic acid, fulvic acid, and bone collagen are often co-extracted with aDNA and inhibit amplification by polymerase chain reaction (PCR). In this study, we compared with two methods of DNA extraction by phenolchloroform extraction and silica-bead extraction. In addition, we applied new protocol, ultra sonication based silica-bead extraction method to extract aDNA from some ancient human skeletal remains. This method was more effective by both mitochondrial DNA (mtDNA) and amelogenin gene amplification.

• Anatomy and Cell Biology
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• v.56 no.1
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• pp.54-60
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• 2023

Lactase non-persistence (LNP), one of the causes of lactose intolerance, is related to lactase gene associated single nucleotide polymorphisms (SNPs). Since the frequency of LNP varies by ethnic group and country, the research to reveal the presence or absence of LNP for specific people has been conducted worldwide. However, in East Asia, the study of lactase gene associated SNPs have not been sufficiently examined so far using ancient human specimens from archaeological sites. In our study of Joseon period human remains (n=14), we successfully revealed genetic information of lactase gene associated SNPs (rs1679771596, rs41525747, rs4988236, rs4988235, rs41380347, rs869051967, rs145946881 and rs182549), further confirming that as for eight SNPs, the pre-modern Korean people had a lactase non-persistent genotype. Our report contributes to the establishment of LNP associated SNP analysis technique that can be useful in forthcoming studies on human bones and mummy samples from East Asian archaeological sites.

• Journal of architectural history
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• v.26 no.5
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• pp.19-26
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• 2017

This paper aimed to identify the architectural characteristics of the chimi excavated from ancient ruins, especially middle gate remains of Bunhwangsa Temple in the Unified Silla period. Middle gate had planned $3{\times}2$ with 295mm measuring unit and gabled building. Detailed study of the shape of chimi of the middle gate restored by related field experts reveals as follows. 1) Height of chimi is 4.5(130.6cm) of Tang's system of measurement classified as large one. 2) The front and back side square hole is made for transverse timber placement. 3) Position of chimi is not the end of the ridge of roof, it moved toward the center. 4) Construction method of chimi is structured with smaller beam and center column. 5) Width and height of the ridge is less than 38cm and 54cm to be approximately, Width and height of the gabled ridge is less than 38cm and 50cm. 6) This chimi is considered to be very unique when compared to existing ancient restored chimis, it is designed to be advantageous to the chimi construction.

• Journal of the Korean Institute of Rural Architecture
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• v.4 no.1
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• pp.45-60
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• 2002

This research is the second attempt to illustrate the phases of ancient Korean architecture through a comparative study of historical materials such as historical ruins, relics, stone remains and art products. Among these the stone stupa (budo), contains many architectural aspects of old times, which give clues to trace the characteristics of the antient Korean structures. The study brings about following results: 1. The pillar employs an entasis technique, and the bracket system (gongpo) consists of cross beams (changbang) and small supporting blocks (soro) thereupon. A type of the bracket arm (chomcha) is also found on top of the pillar. 2. Windows and doors are assumed from the carved features on the stone stupa to be pair-doors which could be folded twice sideways and upwards. 3. There are two types of eaves; the single eaves and the double eaves consisting of a rafter and a flying rafter. 4. The roof has furrows formed by the concave tiles and the convex ones and also the tiles at the edge of eaves. And there are evidences that a variety of small images of animal and human figures were decorated along on top of the eaves.