• 수산해양교육연구
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• 제23권4호
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• pp.607-614
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• 2011

This study was conducted to understand the quality characteristics of 20 kinds of commercial salt-fermented anchovy & sand lance sauces by measuring their the chemical compositions and 9 biogenic amines (tryptamine, 2-phenylethylamine, putrescin, cadaverine, histamine, tyramine, serotonin, noradrenaline, spermine) contents. The commercial salt-fermented anchovy sauces contained $65.84{\pm}0.11{\sim}70.60{\pm}0.21%$ of moisture, $20.50{\pm}0.41{\sim}25.60{\pm}0.42%$ of salinity, $0.98{\pm}0.01{\sim}2.05{\pm}0.05%$ total nitrogen, and $1,011.77{\pm}0.00{\sim}1,724.56{\pm}9.72mg/100mL$ of amino nitrogen. Histamine was the major amine detected in salt-fermented fish sauces and it was varied from 421.27 to 1,507.18 mg/kg in salt-fermented anchovy sauces, whereas commercial salt-fermented sand lance sauces contained $67.87{\pm}0.28{\sim}69.63{\pm}0.17%$ moisture, $22.46{\pm}0.28{\sim}26.11{\pm}0.00%$ salinity, $0.92{\pm}0.01{\sim}1.71{\pm}0.05%$ total nitrogen, $878.20{\pm}0.00{\sim}1430.09{\pm}9.77mg/100mL$ amino nitrogen and 419.10~1,025.50 mg/kg histamine, respectively. These findings suggest that the products of salt-fermented fish sauces have pretty much the same in ingredient composition and meet domestic criteria but most those had high biogenic amine contents. Therefore, ingredient composition and biogenic amine content of commercial salt-fermented fish sauce products were practicable evaluation of the quality characteristics.

• 한국수산과학회지
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• 제52권1호
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• pp.19-26
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• 2019

The objective of this study was to analyze bacteriological and chemical hazards in minimally processed commercial fish products, including Hwangtae (freeze-dried pollock), dried anchovy, fermented anchovy sauce, and salted and dried yellow croaker. Escherichia coli counts from all samples were below the regulation limits of the Korean Ministry of Food and Drug Safety Standards on Quality of Seafood and Seafood Products (Food Code). However, the food poisoning bacterium Staphylococcus aureus was detected at levels above $1.0{\times}10^2$ colony forming units (CFU)/g in Hwangtae, dried anchovy, and salted and dried yellow croaker, which are commonly ingested without heating and pose bacteriological hazards. The detection of S. aureus, an organism indicative of poor personal hygiene, which can be introduced by employees and multiply during distribution, indicates the necessity of improving the sanitary control of minimally processed commercial fish products. Histamine was not detected from dried anchovy or salted and dried yellow croaker, but was detected at some of the highest levels in fermented anchovy sauces. This result suggests that efforts to reduce the amount of histamine in fermented anchovy sauces are required.

• 한국식품영양과학회지
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• 제35권1호
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• pp.87-95
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• 2006

새우가공부산물의 효율적 이용과 멸치 액젓의 영양성 및 기호성의 강화 목적으로 새우가공부산물 첨가 멸치액젓 (새우가공부산물 무첨가 및 $10\%$ 첨가 멸치액젓의 경우 270일 숙성, $20\%$ 및 $30\%$ 첨가 멸치액젓의 경우 180일 숙성)을 제조하여 엑스분 질소, 맛성분, 영양성분 및 관능검사에 대하여 분석 검토하였다. 멸치액젓의 엑스분 질소함량은 새우가 공부산물의 첨가량이 증가할수록 증가하는 경향을 나타내었으며, 맛의 주요성분을 살펴보기 위하여 omission test를 실시한 결과, 새우가공부산물의 첨가 유무에 관계없이 맛의 주성분은 유리아미노산이었고, ATP 관련물질 및 유기산 등의 성분은 맛에 보조적으로 관여하리라 판단되었다. 새우가 공부산물 첨가 멸치액젓의 유리아미노산 및 taste value의 결과, 멸치 액젓의 맛은 모두 glutamic acid와 aspartic acid에 의하여 좌우됨을 알 수 있었고, 첨가비율이 증가할수록 proline, valine 및 histidine의 함량과 조성비가 감소하는 경향인 반면에, methionine, isoleucine, leucine, phenylalanine 및 lysine은 증가하는 경향이었다. 총 아미노산은 새우가공부산물의 첨가량에 관계없이 $9,840\~10,325$ mg/100 g 범위였으며, 무기질 함량은 새우가공부산물의 첨가량이 증가할수록 칼슘, 인, 철 및 칼륨 모두가 증가하는 경향을 나타내어 새우가공부산물의 첨가에 기인함이 확인되었다. 또한 새우가공부산물 첨가 멸치액젓의 향, 맛 및 냄새에 대한 관능검사 결과, 쓴맛이 느껴지지 않으면서 새우 특유의 향을 느낄 수 있는 $20\%$ 첨가 제품이 가장 적절하였다. 멸치액젓의 제조시 새우가공부산물을 마쇄한 다음 첨가하여 숙성시키는 경우 맛, 향 및 영양 개선은 물론이고, 수산가공자원의 효율적인 이용 측면에서 의미 있는 결과를 얻었다.

• 생명과학회지
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• 제5권4호
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• pp.203-210
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• 1995

Fibrinolytic activity of fermented kimchi and ingredients used for the preparation were assayed by measuring the lysis area on plasminogen-rich fibrin plate. Fermented kimchi and picked fish sauces from changlan, prwan, and anchovy showed the activity, and the activity of pickled fish sauces were high in the order of pickled changlan, picked anchovy(pickled mulchi), and pickled prawn. However, the activity of kimchi may not be attributed to pickled fish sauce because kimchi containing fish sauce did not possess activities were determined from the samples heated for 30min at 100$\circ$C. There was no changes in activities before and after heat treatment indicating the agents may be components other than protein. Since major changes occur during kimchi fermentation were increased sour taste due to production of organic acids such as lactic, citric, oxalic, and succinic acids, the authentic organic acids present were examined for fibrinolytic activities. The results indicates that the major component posses the activity is lactic acid.

• 한국식품영양과학회지
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• 제33권7호
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• pp.1192-1200
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• 2004

감마선 조사(10 kGy)된 멸치 액젓의 유전독성 학적 안전성 시험을 수행하기 위해 Salmonella Typhimurium TA98, TA100, TA1535, TA1537과 E. coli WP2 uvrA 균주를 사용한 복귀돌연변이시험과 Escherichia coli PQ37을 이용한 SOS chromotest 및 CHL 세포를 이용한 염색체 이상시험을 활성대사효소계 미적용 및 적용하에 실시하였고, ICR마우스의 골수세포를 이용한 in vivo 소핵세포실험을 수행하였다. 감마선 조사(10kGy)된 멸치액젓은 위의 3가지 in vitro실험에서 비조사 된 멸치액젓과 마찬가지로 음성으로 나타났다. 또, 감마선 조사 및 비조사된 멸치 액젓의 in vivo소핵세포실험에서도 소핵이 발견되지 않았다. 따라서 10kGy까지 감마선 조사된 멸치 액젓은 위 수행된 in vitro 및 in vivo 유전독성 시험을 실시한 결과, 음성을 나타낸 것으로 보아 유전독성학적으로 돌연변이원성이 없는 것으로 확인되었다.

• 한국식품위생안전성학회지
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• 제12권4호
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• pp.333-339
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• 1997

The effects of kind of vegetables and of the kind and amounts of fermented fish sauce on the formation of nitrosamine (NA) during kimchi fermentation were investigated. Kimchies made of Chinese cabbage, cucumber, and radish with fermented shrimp, anchovy and liquid sauces were fermented at 4$^{\circ}C$ for 6 weeks and the changes in the content of nitrate, nitrite, trimethylamine (TMA), dimethylamine (DMA) and NA were studied. Nitrate content in kimchies made of Chinese cabbage, cucumber, and radish increased at the initial period of fermentation, but it decreased at the later period. Nitrite was not detected at the later period of kimchi fermentation. Overall, there have not been great changes in the contents of nitrite and nitrate. However, there have been considrable changes in the contents of TMA and DMA as fermentation progressed. Only nitrosodimethylamine (NDMA) at the level of 0.5~10.3 $\mu\textrm{g}$/kg was formed in three kinds of kimchies. More NDMA was formed in radish and cucumber kimchies than in Chinese cabbage kimchi. The pH was lowered faster in radish and cucumber kimchies than in Chinese cabbage kimchi. More NDMA was formed in Chinese cabbage kimchi made with fermented shrimp sauce than those with anchovy or liquid sauces. Shrimp sauce contained higher amount of DAM than anchovy and liquid sauces. The contents of NDMA tended to increase as the amount of shrimp sauce increased. The kind and amount of fermented fish sauce used for kimchi preparation may be an important factor affecting the formation of NDMA.

• 한국수산과학회지
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• 제33권5호
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• pp.383-387
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• 2000

액젓을 분광광도계로 최대 흡수파장을 scanning 하고, 12개월 숙성된 멸치액젓에 glucose, MSG, IMP, 카라멜색소 및 타르색소를 첨가하여 저장기간에 따라 색도의 변화를 측정하여 액젓의 품질 판정을 위한 지표로서의 사용가능성을 조사한 결과는 다음과 같다. 액젓의 종류별, 희석비별, 첨가색소별, 첨가물별에 따라서 scanning한 결과, 453nm에서 최대 흡광도를 나타내었고, 분광광도계로 453 nm에서 측정한 시판 멸치액젓의 색도는 직시색차계로 측정한 ${\delta}E$값 (색차)보다 감도가 더 좋았으므로, 액젓의 색도 측정시 직시색차계보다는 분광광도계로 453 nm에서 측정하는 것이 더 적합할 것으로 판단되었다. 숙성기간 중에는 색도값이 일정하게 증가하였으며, 까나리액젓이 멸치액젓보다 그 값이 높았다. 첨가물의 영향은, 당 (glucose)과 질소화합물 (MSG, IMP)을 첨가한 시료는 저장기간 동안 색도값이 일정하게 증가 하였으며, 농도별에 따른 큰 차이는 없었다. 색소의 영향은, 카라멜색소를 첨가한 액젓의 색도값은 첨가농도가 높을수록 높았으며, 저장기간중에 일정하게 증가하였다. 한편, 타르색소를 첨가한 액젓은 첨가농도 $0.003{\%}$ 및 $0.005{\%}$에서 저장 15일 후에 색도값이 급격히 증가하였으며, 저장기간 중에도 일정하게 증가하였다.

• 한국수산과학회지
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• 제31권3호
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• pp.386-392
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• 1998

액젓의 객관적인 품질평가 기준을 설정하기 위하여, 전통적인 방법에 따라 발효 숙성시킨 멸치 액젓과 시판 멸치 액젓의 일반성분, 총질소량, 아미노산 함량, SDS-PAG전기영동을 통한 특정 펩티드의 면적을 측정하였고, 아울러 액젓 원료어의 판정을 위해 등전점 전기영동을 시도하였다. 멸치 액젓의 수분 함량은 대조구가 가장 낮은 $60.5\%$였으며, 시판 제품은 $65.5\%\~69.2\%$로 큰 차이가 없었으나, 총질소 함량은 시판 제품간에 비교적 큰 차이를 보이고 있었다. 액젓에 잔존하는 단백질의 함량은 Y사의 제품이 비교적 높은 $0.353mg/m{\ell}$ 였으며, 나머지 시판 제품은 $0.093mg/m{\ell}\~0.293mg/m{\ell}$의 범위였다 한편 SDS-PAG 전기영동상에서는 J사를 제외한 모든 액젓 제품에서 분자량 55,600 및 46,900 dalton의 band가 검출되었으며, 전기영동상에 나타난 단백질 band는 제조회사에 관계없이 거의 비슷한 형태를 나타내고 있었다. 그리고 등전점 전기영동에서 멸치 액젓은 등전점 5.6에 해당하는 펩티드가 공통적으로 존재하며, 특징적인 band임을 확인하였다. 따라서 아미노산 및 저분자 질소 화합물의 첨가 여부가 정량적인 값에 영향을 미치지 않는 전기영동 상의 특정 band의 면적비를 측정함으로서 인위적인 질소화합물의 첨가, 액젓 희석 여부 판정이 가능 할 것으로 판단되었다. 그리고 전 질소 함량, 아미노산 함량 및 SDS-PAG 전기영동 상에 있는 특징적인 55,600 dalton에 해당하는 펩티드 면적비의 상관관계는 멸치 액젓의 품질판정을 위한 기준으로 활용 가능함을 제시하고 있다.

• 한국식품영양과학회지
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• 제29권5호
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• pp.837-842
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• 2000

Commercially distributed salted and fermented anchovy sauces in Korea were purchased from six different companies. Microbiological, chemical, rheological and sensory analyses were carried out to evaluate the quality and safety and to provide basic information of the products. The salinity of every product was lower than of regulatory standard. All samples tested had acceptable in moisture content and pH. However, wide range of amino nitrogen (AN), volatile basic nitrogen(AN), volatile basic nitrogen (VBN), and trimethylamine (TMA) contents were observed. One product among tested had lower level of AN than regulatory standard. Pathogenic microorganisms were also detected from the samples, therefore sanitation procedure will be needed to ensure safety. From the results, AN, VBN, and viscosity were highly correlated to sensory scores, suggesting that these values can utilized as a standard method to evaluate quality of the salted and fermented anchovy sauce.

• 한국식품영양학회지
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• 제28권2호
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• pp.189-195
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• 2015

Failure of the functions involved in ingestion leads to, not only loss of enjoyment of eating, but also protein-energy malnutrition. Dysmasesis and difficulty of swallowing occurs in various diseases, but aging is a major cause. In the aging society, the proportion of elderly people with dysmasesis and difficulty of swallowing is expected to increase rapidly. Developing foods for the elderly that are easy to chew and swallow is thus required. This study was conducted to develop easily chewable and swallowable foods for the elderly who can crush foods and ingest with their own tongues, but occasionally have difficulty in drinking fluids such as water and tea. Various foods for the elderly with chewing and swallowing difficulties were used for sensory assessment. The sensory panel consisted of 10 dietitians (10 women) in nursing care facilities. The sensory optimal composite recipes were determined by central composite design (CCD). The sensory measurements were significantly different in saltiness (p<0.05), sweetness (p<0.05), and overall quality (p<0.05). The optimum formulation of stir fried anchovy calculated by numerical and graphical methods was 3.74 g of soy sauces and 30.17 g of oligo-saccharides. Stir fried anchovy had a moisture content, hardness and adhesiveness of 76.52%, 2.10, and -1.57, respectively.