• Title/Summary/Keyword: Analytical precision

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Development of Jaspine B analysis using LC-MS/MS and its application: Dose-independent pharmacokinetics of Jaspine B in rats

  • Song, Im-Sook;Jeon, Ji-Hyeon;Lee, Jihoon;Lim, Dong Yu;Lee, Chul Haeng;Lee, Dongjoo;Choi, Min-Koo
    • Analytical Science and Technology
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    • v.34 no.2
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    • pp.37-45
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    • 2021
  • A rapid and simple LC-MS/MS analytical method in determining Jaspine B has been developed and validated in rat plasma. The standard curve value was 25 - 5000 ng/mL and the linearity, inter-day and intra-day accuracy and precision were within 15.0 % of relative standard deviation (RSD). The mean recoveries of Jaspine B ranged from 87.5 % to 91.2 % with less than 3.70 % RSD and the matrix effects ranged from 91.1 % to 108.2 % with less than 2.6 % RSD. The validated LC-MS/MS analytical method of Jaspine B was successfully applied to investigate the dose-escalated pharmacokinetic study of Jaspine B in rats following an intravenous injection of Jaspine B at a dose range of 1 - 10 mg/kg. The initial plasma concentrations and area under plasma concentration curves showed a good correlation with intravenous Jaspine B dose, indicating the dose independent pharmacokinetics of Jaspine B in rats. In conclusion, this analytical method for Jaspine B can be easily applied in the bioanalysis and pharmacokinetic studies of Jaspine B, including its administration at multiple therapeutic doses, or for making pharmacokinetic comparisons for the oral formulations of Jaspine B in small experimental animals as well as in vivo pharmacokinetic-pharmacodynamic correlation studies.

Qualitative and quantitative determination of oleanolic acid in a scalp tonic products by HPLC using response surface methodology for extraction optimization

  • Cai, Lin Xi;Cho, Chong Woon;Zhao, Yan;Kang, Jong Seong;Kim, Kyung Tae;Jung, Sang-Hun
    • Analytical Science and Technology
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    • v.32 no.2
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    • pp.48-55
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    • 2019
  • The simple and effective analytical method for the quality control of a novel scalp tonic formulation has been developed and optimized in terms of HPLC conditions and sample preparation method, meanwhile, the optimization of preparation condition was using response surface methodology (RSM) based on central composite design (CCD). Oleanolic acid was selected as marker compound because of its bioactivities for alopecia therapy. The developed analytical method and extraction condition were successfully qualified. Coefficient of determination ($r^2$) for the calibration was 0.9997 with a line passing through the origin point in the range of 0.1-100 mg/mL. The limit of detection (LOD) and the limit of quantitation (LOQ) were 17.5 ng/mL and 55.0 ng/mL, respectively. The intra-day and inter-day precision of the method were 0.5-1.4 % and 0.7-1.8 % in relative standard deviation, respectively, while those accuracy were 99.5-100.9 % and 100.0-102.2 %, respectively. The repeatability of oleanolic acid in samples ranged of 0.3-1.9 % based on peak area and 0.3-0.7 % for retention time. Recoveries from samples were 95.0-99.4 % with lower than 1.8 % in relative standard deviation. Overall, the developed analytical method will be used for quality control of this commercial scalp tonic products successfully.

A simple analytical model for free vibration and buckling analysis of orthotropic rectangular plates

  • Sellam, Souad;Draiche, Kada;Tlidji, Youcef;Addou, Farouk Yahia;Benachour, Abdelkader
    • Structural Engineering and Mechanics
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    • v.75 no.2
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    • pp.157-174
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    • 2020
  • In the present paper, a simple analytical model is developed based on a new refined parabolic shear deformation theory (RPSDT) for free vibration and buckling analysis of orthotropic rectangular plates with simply supported boundary conditions. The displacement field is simpler than those of other higher-order theories since it is modeled with only two unknowns and accounts for a parabolic distribution of the transverse shear stress through the plate thickness. The governing differential equations related to the present theory are obtained from the principle of virtual work, while the solution of the eigenvalue problem is achieved by assuming a Navier technique in the form of a double trigonometric series that satisfy the edge boundary conditions of the plate. Numerical results are presented and compared with previously published results for orthotropic rectangular plates in order to verify the precision of the proposed analytical model and to assess the impacts of several parameters such as the modulus ratio, the side-to-thickness ratio and the geometric ratio on natural frequencies and critical buckling loads. From these results, it can be concluded that the present computations are in excellent agreement with the other higher-order theories.

Evaluation of Analytical Method for Determination of Potassium in Tobacco Leaf (담배 엽 중 칼륨 분석법의 평가)

  • Cho Sung-Eel;Kim Mi-Ju;Kim Sang-Un;Kim Yong-Ha;Min Young-Keun
    • Journal of the Korean Society of Tobacco Science
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    • v.28 no.1
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    • pp.51-57
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    • 2006
  • This study was carried out to improve and evaluate the analytical method for determination of potassium in tobacco leaf by various pre-treatment techniques. The time requirment of various pre-treatments was about 10 hour for dry ashing and 6 hour for microwave digestion and 3 hour for sonication. The results of recover in both pre-treatment techniques, microwave digestion and sonication, is greater than 85 % stably with reproducibility(RSD %) on replicates of less than 3 %. However the mean values for microwave digestion were lower than certified standard value of NlST SRM. Compared to the other pre-treatment techniques, analytical results for sonication technique strictly improved the accuracy and precision. In conclusion, the use of the simple sonication technique seems to be efficient for the determination of potassium in tobacco leaf in consideration for both the accuracy and reproducibility.

Application of Methane Mixed Plasma for the Determination of Ge, As, and Se in Serum and Urine by ICP/MS

  • Park, Kyung-Su;Kim, Sun-Tae;Kim, Young-Man;Kim, Yun-je;Lee, Won
    • Bulletin of the Korean Chemical Society
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    • v.24 no.3
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    • pp.285-290
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    • 2003
  • An analytical method for the simultaneous determination of trace Ge, As and Se in biological samples by inductively coupled plasma/mass spectrometry has been investigated. The effects of added organic gas into the coolant argon gas on the analyte signal were studied to improve the detection limit, accuracy and precision. The addition of a small amount of methane (10 mL/min.) into the coolant gas channel improved the ionization of Ge, As and Se. The analytical sensitivity of the proposed Ar/CH₄system was superior by at least two-fold to that of the conventional Ar method. In the present method, the detection limits obtained for Ge, As and Se were 0.014, 0.012 and 0.064 ㎍/L, respectively. The analytical reliability of the proposed method was evaluated by analyzing the certified standard reference materials (SRM). Recoveries of 99.9% for Ge, 103% for As, 96.5% for Se were obtained for NIST SRM of freeze dried urine sample. The proposed method was also applied to the biological samples.

Trifluralin in aquatic products: QuEChERS and Gas chromatography-tandem mass spectrometry for trace amount detection

  • Le-Thi Anh-Dao;Do Minh-Huy;Vo Hong-Phong;Nguyen Cong-Hau
    • Analytical Science and Technology
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    • v.36 no.5
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    • pp.205-215
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    • 2023
  • In the present study, an analytical method was proposed for detecting trifluralin in aquatic products at trace concentrations. The method employed QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) and gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) as the sample preparation and measurement, respectively. The effect of the aqueous phase volume used in the QuEChERS was demonstrated, and the ratio of 10:10 (mL) between water and acetonitrile phase was used for 5 g of sample. Besides, dSPE using C18 and primary-secondary amine (PSA) was applied to remove the potential interferences from the food matrices, indicating no remarkable analyte loss. The linear range was built up from 0.50 ㎍ L-1 to 3.0 ㎍ L-1 (R2 = 0.9993). Other criteria, i.e., repeatability (RSDr = 0.86-1.96 %), reproducibility (RSDR = 1.09-2.01 %), and recovery (over 90 %), were in accordance with Appendix F of AOAC (2016) for method performance. Although no trifluralin was detected for the commercial samples (fish, shrimp, and breaded shrimp), the spiked samples performed favorable recoveries and precision.

A study on selenium quantification using ICP-MS with oxygen reactive gas in soil of Korea

  • Hyun-Young Kim;Young-Kyu Hong;Jin-Wook Kim;Sung-Chul Kim
    • Analytical Science and Technology
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    • v.37 no.4
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    • pp.230-238
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    • 2024
  • This study evaluates a method for quantifying selenium (Se) concentration in soil using inductively coupled plasma mass spectrometry (ICP-MS), with oxygen as a reaction gas. This approach addresses the challenge of detecting low levels of Se in complex soil matrices and aims to effectively minimize interference problems typically associated with argon plasma in traditional ICP-MS analyses. The analytical method utilizes conditions optimized for minimizing spectral interference and were validated by linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). The method demonstrated good linearity, high accuracy (90-97 %), and remarkable sensitivity, achieving detection and quantification limits of 0.15 ㎍/kg and 0.44 ㎍/kg, respectively. Developed analysis method for Se in soil was applied to field samples in the different regions of South Korea and Se concentration ranged from 0.11 to 0.52 mg/kg. Correlation analysis between Se concentration and soil properties showed that Se concentration was significantly correlated with cation exchange capacity (CEC) and available phosphorus among other soil properties.

DEVELOPMENT OF A HYBRID CFD FRAMEDWORK FOR MULTI-PHENOMENA FLOW ANALYSIS AND DESIGN (다중현상 유동 해석 및 설계를 위한 융복합 프레임웍 개발)

  • Hur, Nahm-Keon
    • 한국전산유체공학회:학술대회논문집
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    • 2010.05a
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    • pp.517-523
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    • 2010
  • Recently, the rapid evolution of computational fluid dynamics (CFD) has enabled its key role in industries and predictive sciences. From diverse research disciplines, however, are there strong needs for integrated analytical tools for multi-phenomena beyond simple flow simulation. Based on the concurrent simulation of multi-dynamics, multi-phenomena beyond simple flow simulation. Based on the concurrent simulation of multi-dynamics, multi-physics and multi-scale phenomena, the multi-phenomena CFD technology enables us to perform the flow simulation for integrated and complex systems. From the multi-phenomena CFD analysis, the high-precision analytical and predictive capacity can enhance the fast development of industrial technologies. It is also expected to further enhance the applicability of the simulation technique to medical and bio technology, new and renewable energy, nanotechnology, and scientific computing, among others.

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A Study on Analytical Method of Phthalate Esters in Water by SPE(Solid-Phase-Extraction) (고체상 추출법을 이용한 Phthalate Esters의 분석방법 연구)

  • 홍성희;한개희;이찬형;이순화
    • Journal of Environmental Science International
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    • v.12 no.1
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    • pp.81-86
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    • 2003
  • The study was carried out to evaluate the new analytical method of phthalate esters(diethylphthalate, di-n-butylphthalate, butylbenzylphthalate, bis(2-ethylhexyl)phthalate), one of the endocrine disruptors, which were performed by GC/MS-SIM(selected ion monitoring). The phthalate esters were extracted from water samples using solid-phase extraction on $C_{18}$ columns. It investigated that the extraction recovery rate of phthalate esters with different solvents and solvent volume. The optimal solvent was dichloromethane and proper volume of dichloromethane for recovery of phthalate esters was 4 mL. There were good linearities(above $R^2$=0.9975) in the range 0.01~0.50mg/L, and the detection limits were below 0.01~0.03$\mu\textrm{g}$/L. The recovery rates, RSD and MDLs for phthalate esters were 80~114%, 5.0~8.1% and 0.03~0.11$\mu\textrm{g}$/L, respectively. This method shows a good precision of phthalate esters.

Analysis of orthotropic plates by the two-dimensional generalized FIT method

  • Zhang, Jinghui;Ullah, Salamat;Gao, Yuanyuan;Avcar, Mehmet;Civalek, Omer
    • Computers and Concrete
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    • v.26 no.5
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    • pp.421-427
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    • 2020
  • In this study, the two-dimensional generalized finite integral transform(FIT) approach was extended for new accurate thermal buckling analysis of fully clamped orthotropic thin plates. Clamped-clamped beam functions, which can automatically satisfy boundary conditions of the plate and orthogonality as an integral kernel to construct generalized integral transform pairs, are adopted. Through performing the transformation, the governing thermal buckling equation can be directly changed into solving linear algebraic equations, which reduces the complexity of the encountered mathematical problems and provides a more efficient solution. The obtained analytical thermal buckling solutions, including critical temperatures and mode shapes, match well with the finite element method (FEM) results, which verifies the precision and validity of the employed approach.