• 한국식품위생안전성학회지
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• 제36권3호
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• pp.257-263
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• 2021

식품별 알레르기 유발물질 표시사항을 모니터링하기 위해 돼지고기, 우유 등 14개의 종 특이 프라이머를 이용한 유전자 검출법(PCR)을 실시하였다. 초등학교 근처 문구점과 수입과자판매점을 대상으로 알레르기 유발물질에 대한 표시사항이 없는 과자, 캔디류, 초콜릿류 등 60건에 대한 검사를 진행하였으며 그 중 30건에서 우유, 밀, 달걀, 토마토, 아몬드, 땅콩이 검출되었다. 특히 수입제품에서는 알레르기 유발물질을 표시하지 않았거나 한글 표시가 없는 경우도 확인되었으며, 표시 항목 이외의 물질이 검출되는 등 표시 사항이 미흡한 제품들이 다수 확인되었다. 소비자들이 안심하고 제품을 구매할 수 있도록 알레르기 유발물질 표시 사항에 대해 국내 제조가공업체와 수입관련 업체의 계도 및 감시 등 관련 기관 간의 긴밀한 협조 체계가 유지되어야 할 것이다.

• 대한미생물학회지
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• 제3권1호
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• pp.51-65
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• 1968

The site of the synthesis of Japanese encephalitis virus(JEV) in the actinomycin-treated and infecter PS Y15 cells(a porcine kidney stable cell line) was observed by the immunofluorescent antibody technique, acridine orange staining, and the autoradiographic analysis. In the parallel studies by immunofluorescent technique and acridine orange staining it the infected cells, Viral protein(as an antigen) and viral RNA were detected at the same site of cytoplasm. In the autoradiographic analysis, the cytoplasmic labeling of $^3H$-uridine was due to the synthesis of JEV-RNA, while the nucleolus and nucleus were not involved. In the autoradiographic studies on the secton of infected cells, the $^3H$-uridine was frequently incorporated around the cytoplasmic vacuoles. This localization of labeling agreed with the site of acridine orange positive granules. The results suggest that the syntheses of the viral RNA and viral protein occurred in the similar site of cytoplasm of the infected cells, and also the virus particles seem to be assembled in the sites of the viral RNA and protein syntheses.

• 한국가축번식학회지
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• 제19권2호
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• pp.95-104
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• 1995

Mouse mammary epithelial cells(NMuMG) were maintained onto 6-well plates (3$\times$105 cells/well) or chambered slide (1$\times$104 cells/well), in DMEM supplemented with 10% fetal calf serum. After serum starvation for 24 hours, DMNB (1$\mu$M) was added and exposed to UV light (300nm, 3 second pulse) after 2 hours from DMNB addition in order to activate DMNB which induces a rapid transient increase in intracellular cAMP upon UV irradiation. EGF (100ng/ml) and/or IGF-I (10ng/ml) were treated at the time of UV irradiation. Nuclear labeling index was estimated as percent of nuclear labeled cells(percent of S phase of cells) by incorporation of 3H-thymidine into DNA(1 hour pulse with 1$\mu$Ci/ml). DMNB(1$\mu$M), EGF (100ng/ml) and/or IGF-I (10ng/ml) signifciantly increased nuclear labeling index than those of control (P<0.05). Addition of DMNB＋EGF or DMNB＋EGF＋IGF-I showed the interaction effect in nuclear labeling index (P<0.05). Protein kinase A activities by addition of EGF, IGF-I or EGF＋IGF-I were 10.5, 9.8 or 9.4 unit/mg protein, respectively, and no statistical difference was found in comparison with control (P>0.05). Additon of DMNB＋EGF showed the moderate interaction effect on tyrosyl kinase activity (P<0.1). In the fluorography analysis, there were no specific protein phosphorylation patterns were found at 1 or 15 minute by addition of DMNB. EGF and/or IGF-I. These results suggest that the interaction effect in nuclear labeling index by addition DMNB and EGF could be mediated through the modulation of tyrosyl kinase activity by cAMP.

• 한국정보통신학회논문지
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• 제20권4호
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• pp.826-832
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• 2016

본 논문에서는 고가의 디지털 설진 장비와 특별한 장치 없이 누구나 손쉽게 사용할 수 있는 디지털 설진 시스템의 첫 단계로 미각 영역별 균열 유무를 판별하는 시스템을 제안한다. 훈련 DB는 한방 병원에서 수집한 사진 261장을 바탕으로 Haar-like feature, Adaboost 학습을 하였다. 학습된 결과를 통하여 입력영상으로부터 혀 후보영역을 검출하고, 검출된 혀 후보영역으로부터 혀 영역만을 분리하기 위하여 261장의 훈련 DB의 HSV 컬러모델의 Hue 성분 평균 값을 산출하였다. 검출된 혀 윤곽으로부터 Connected Component Labeling을 통하여 혀 영역을 분리 하였다. 분리된 혀 영역의 상대적 너비와 높이를 이용하여 미각 영역별 로 분할하였다. 분할된 미각 영역별 영상은 Gray영상으로 변환하고, 각각의 영역별 평균 밝기를 산출하여 이진화하였다. 이진화 영상에 Connected Component Labeling을 통하여 균열 유무를 판별하였다.

• ETRI Journal
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• 제36권3호
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• pp.429-438
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• 2014

Semantic role labeling (SRL) is a task in natural-language processing with the aim of detecting predicates in the text, choosing their correct senses, identifying their associated arguments, and predicting the semantic roles of the arguments. Developing a high-performance SRL system for a domain requires manually annotated training data of large size in the same domain. However, such SRL training data of sufficient size is available only for a few domains. Constructing SRL training data for a new domain is very expensive. Therefore, domain adaptation in SRL can be regarded as an important problem. In this paper, we show that domain adaptation for SRL systems can achieve state-of-the-art performance when based on structural learning and exploiting a prior model approach. We provide experimental results with three different target domains showing that our method is effective even if training data of small size is available for the target domains. According to experimentations, our proposed method outperforms those of other research works by about 2% to 5% in F-score.

• 한국조리학회지
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• 제23권5호
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• pp.129-139
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• 2017

The necessity of selecting food based on correct information through nutrition ingredients included in the food is being emphasized due to the recent increase in interest of restaurant users in healthy and safe food. This study verified the structural causal relationship among consumer attitude, use reason and health-consciousness regarding nutrition information labeling and their satisfaction and revisit intent. A total of 266 surveys conducted over restaurant users residing in the Busan area was used for the final analysis of the study to attain this study's objective. The suitability of the Structural Equation Modeling and organic causation relationship was verified through the statistics programs SPSS (V 23.0) and AMOS (V 21.0) in order to attain this study's objective, and the control effect of age has been verified. The results showed that a meaningful causal relationship was confirmed in all elements excluding consumer attitude. A control effect was also confirmed in the influential relationship between use reason and satisfaction level. The results of the study suggested that the nutrition information labeling system of restaurants provides practical implications and marketing strategies for restaurant managers and government policy makers.

• Molecular & Cellular Toxicology
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• 제1권2호
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• pp.92-98
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• 2005

DNA microarray analysis of gene expression in toxicogenomics typically requires relatively large amounts of total RNA. This limits the use of DNA microarray when the sample available is small. To confront this limitation, different methods of linear RNA amplification that generate antisense RNA (aRNA) have been optimized for microarray use. The target preparation strategy using amplified RNA in DNA microarray protocol can be divided into direct-incorporation labeling which resulted in cDNA targets (Cy-dye labeled cDNA from aRNA) and indirect-labeling which resulted in cRNA targets (i.e. Cy-dye labeled aRNA), respectively. However, despite the common use of amplified targets (cDNA or cRNA) from aRNAs, no systemic assessment for the use of amplified targets and bias in terms of hybridization performance has been reported. In this investigation, we have compared the hybridization performance of cRNA targets with cDNA targets from aRNA on a 10 K cDNA microarrays. Under optimized hybridization conditions, we found that 43% of outliers from cDNA technique and 86% from the outlier genes were reproducibly detected by both targets hybridization onto cDNA microarray. This suggests that the cRNA labeling method may have a reduced capacity for detecting the differential gene expression when compared to the cDNA target preparation. However, further validation of this discordant result should be pursued to determine which techniques possesses better accuracy in identifying truly differential genes.

• 산업식품공학
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• 제22권4호
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• pp.283-294
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• 2018

Food allergy is a chronic disease that is increasing all over the world, and it can even lead to a loss of life. To prevent any incidents resulting from food allergies, most countries keep strengthening their food allergen labeling requirements domestically and internationally, with a constant monitoring system against undeclared allergens and recall of offending products. In order to avoid economic losses to industry and damages to international relations from undeclared allergens, it is necessary to confirm each country's regulatory policy on food allergen labeling prior to exportation. Another required action is to try for a reduction of the cross-contamination risk of the allergens during manufacturing and storage, which should be verified by using an accurate and reliable analysis of food allergens. This paper is intended to provide an introduction to the regulation of food allergen labeling by country, allergen management methods to avoid cross-contamination, and allergen detection methods using ELISA, PCR, and LC/MS. Changes of allergenicity during thermal or nonthermal processing also will be investigated in our review. This review will be helpful for the food industry to better understand patients suffering from food allergies and to manage food allergens in food manufacturing.

• 한국지역사회생활과학회지
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• 제25권2호
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• pp.175-192
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• 2014

This study explores the effects of various factors on the use of nutrition information on food away from home (FAFH). Consumer groups were classified into groups according to their perception of being overweight and obese (correctly perceiving, underestimating and overestimating weight). For this, frequency analysis, a chi-square test, and an ANOVA were conducted to determine any differences between factors, and a logit analysis (SPSS 18.0) was conducted to identify those factors influencing the use of information. Information recognition, intentions to use information and the use on FAFH showed significant differences across the groups. In addition, health inspection, the perception of the intrinsic quality of restaurants, labeling, FAFH expenditure, and some individual characteristics showed significant differences between groups. The information recognition of FAFH had a significant positive effect on information use in all groups. In all groups, labeling had a significant positive effect on information use, and family health concerns had a significant positive effect on information use in the group correctly perceiving weight. The price of domestic food items, household head and household income had significant positive effects on information use in the group overestimating weight.

• 한국콘텐츠학회논문지
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• 제18권3호
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• pp.648-657
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• 2018

본 연구는 한국인에게 적합한 단백질 바를 개발하기 위하여 한국과 미국의 시판되고 있는 단백질 바의 라벨 분석을 하였다. 또한 조사한 단백질 바의 단백질 함량과 일일단백질섭취량, 단백질 권장섭취량(DRI), 그리고 단백질 에너지섭취분율(AMDR)과 비교 분석하였다. 단백질 바는 두 나라의 오프라인과 온라인 마켓을 통해 한국의 17개 제품과 미국의 113개 제품을 수집하였고, 영양소 함량과 원재료를 조사하였다. 그 결과, 미국의 단백질 바가 한국의 것에 비해 1회 제공량이 더 많기 때문에 전체적인 영양소의 함량도 높았는데, 특히 100g으로 환산하였을 때 단백질과 나트륨의 함량은 큰 차이로 높았다. 원재료를 통한 단백질 소재를 분석하였을 때, 한국은 분리대두단백(SPI)이, 미국은 분리유청단백(WPI)이 가장 많이 이용되었다. 이는 나라별 식품의 선호와 친숙함에 영향을 받은 것으로 보인다. 결론적으로, 두 나라의 식습관과 단백질 급원에 대한 섭취 및 선호가 다르기 때문에 한국인에게 적합한 단백질 바의 개발이 필요할 것으로 사료되며, 본 연구의 결과는 소비자들이 단백질 바를 선택하는 데 도움을 줄 것이다.