• 한국식품위생안전성학회지
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• 제38권6호
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• pp.464-475
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• 2023

본 연구에서는 온라인 마켓에서 판매되는 50개 복어제품의 종판별 및 표시사항 일치여부 모니터링을 수행했다. 복어의 종판별을 위해 cytochrome c oxidase subunit I 및 cytochrome b 유전자의 염기서열을 분석하여 NCBI GenBank 데이터베이스에 등록되어있는 생물종의 염기서열과 비교 후 계통 분석을 수행했다. 참복, 흰점참복, 까치복, 복섬, 검복, 국매리복, 흑밀복 총 7종이 동정되었으며, 35개 제품(70%)에서 표시사항과의 불일치를 나타냈다. 12개의 제품(24%)에서는 식품공전에서 제시한 식용 가능한 복어 21종의 국명 대신 일반명(복어)을 사용하였다. 가공 정도별 불일치율은 다중가공 제품(n=9, 81.8%)이 단순가공 제품(n=26, 66.7%)보다 높은 비율을 보였으며, 원산지별 불일치율의 경우 중국산 제품(n=8, 80%)이 국산 제품(n=26, 66.7%)보다 높은 비율을 보였다. 시장명, 방언 등의 이름이 혼용되어 다수의 복어종을 밀복, 졸복으로 표시하였다. 이러한 분류체계의 어려움으로 인해 흰점참복, 국매리복과 같은 식용불가 복어가 식용 가능한 복어인 졸복으로 혼용되어 판매되는 것이 확인되었다. 따라서 식용 가능한 복어를 정확히 분류할 수 있는 방법의 개발이 필요하며, 수입 및 국내 유통 복어 제품의 주기적인 모니터링이 필요하다.

• 분석과학
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• 제6권4호
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• pp.411-415
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• 1993

지방산을 UV 검출기를 사용하여 HPLC로 분석하기 위하여 2-bromoacetyltriphenylene을 유도체화제로 응용하였다. 18-crown-6-ether를 촉매로 한 유도체화반응은 매우 신속, 간편하고 정량적으로 진행되었으며, HPLC상에서 지방산의 검출한계가 1ng 수준으로 그 감도가 양호하였다. 또한 $C_{12}$부터 $C_{22}$까지 9종류의 포화지방산이 역상컬럼상에서 acetonitrile-물 gradient 조건으로 동시에 분리가 가능하였다. 이를 응용하면 각종 생체 시료로부터 미량의 지방산들에 대한 동시분석이 가능하리라 기대된다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1988년도 한국자동제어학술회의논문집(국제학술편); 한국전력공사연수원, 서울; 21-22 Oct. 1988
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• pp.822-825
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• 1988

In this paper, we present a system which enables a real-time measurement of the number and also the body length of the fry (baby fish) using a line image sensor. Here, we consider a situation that fry are transported from a pond to another, pond through a pipe. At one position of the pipe a transparent rectanglar channel is mounted. The images of the fry, which run through this rectanglar channel, are detected by a line image sensor. The image signals are digitized to binary ones and the contour of the fry are detected. After that, a real-time image analysis is executed with a digital signal processor. Labeling program analyses the connection of every pixel. The results are transfered to a personal computer and displayed on the online monitor graphically.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권3호
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• pp.191-195
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• 2000

Quantitation of N7-methyldeoxyguanosine (N7-MedG) produced in the in vitro N-methly-N-nitrosuourea (NMU) action on thymus DNA has been achieved by enzymatic degradation, liquid chromatoraphic separaphic separation and desorption chemical ionization tandem mass spectrometry. In conjunction with the resolving power HPLC in the separation of isomers, desorprion chemical ionization tandem mass spectrometry has utilized in determining modified nucleosides at low levels using a stable-isotope labled compound as an internal reforence. The quantitative estimation of N7-methyldeoxyguanosine was previously established by an independent HPLC analysis of methylated calf thymus DNA. A sensitive and specific methodogy for the quantitation of N7-MedG at the picomole level using HPLC combined with tandem mass spectrometry without radioisotope labeling process is presented. The potential of the liquid chromatoraphic tandem mass exposure to methlation agents in vitro.

• 한국정보처리학회논문지
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• 제7권3호
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• pp.971-976
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• 2000

In this paper a new method for head gesture recognition is proposed. A the first stage, face image data are transformed into low dimensional vectors by principal component analysis (PCA), which utilizes the high correlation between face pose images. The a self organization map(SM) is trained by the transformed face vectors, in such a that the nodes at similar locations respond to similar poses. A sequence of poses which comprises each model gesture goes through PCA and SOM, and the result is stored in the database. At the recognition stage any sequence of frames goes through the PCA and SOM, and the result is compared with the model gesture stored in the database. To improve robustness of classification, probabilistic relaxation labeling(PRL) is used, which utilizes the contextural information imbedded in the adjacent poses.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1996년도 추계학술대회
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• pp.357-362
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• 1996

A way of quantitatively describing the tissue architecture we have investigated when developing a computer program for malignancy grading of transitional cell bladder carcinoma. The minimum spanning trees, MST was created by connecting the center points of the nuclei in the tissue section image. These nuclei were found by thresholding the image at an automatically determined threshold followed by a connected component labeling and a watershed algorithm for separation of overlapping nuclei. Clusters were defined in the MST by thresholding the edge lengths. For these clusters geometric and densitometric features were measures. These features were compared by multivariate statistical methods to the subjective grading by the pathologists and the resulting correspondence was 85% on a material of 40 samples.

• 반도체디스플레이기술학회지
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• 제9권2호
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• pp.79-85
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• 2010

In this paper, we propose a web inspection system (WIS) for real-time detection of paper defects which can cause critical fractures during papermaking process. Our system incorporates high speed line-scan camera, lighting system, and detection algorithm to provide robust and precise detection of paper defects in real-time. Since edge defects are very crucial to the paper fractures, our system focuses on the edge region of the paper instead of inspecting the whole paper area. In our algorithm, image projection and sub-pixel operation are utilized to detect the edge defects precisely and connected component labeling and shape analysis techniques are adopted to extract various kinds of the region defects. Experimental results revealed that our web inspection system is very efficient for detecting paper defects during papermaking processes.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.263-263
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• 2017

Rice (Oryza sativa L.) is the world's third largest food crop after wheat and corn. Geographic authentication of rice has recently emerged as an important issue for enhancing human health via food safety and quality assurance. Here, we aimed to discriminate rice from six Asian countries through geographic authentication using combinations of elemental/isotopic composition analysis and chemometric techniques. Principal components analysis could distinguish samples cultivated from most countries, except for those cultivated in the Philippines and Japan. Furthermore, orthogonal projection to latent structure-discriminant analysis provided clear discrimination between rice cultivated in Korea and other countries. The major common variables responsible for differentiation in these models were ${\delta}^{34}S$, Mn, and Mg. Our findings contribute to understanding the variations in elemental and isotopic compositions in rice depending on geographic origins, and offer valuable insight into the control of fraudulent labeling regarding the geographic origins of rice traded among Asian countries.

• Journal of Microbiology and Biotechnology
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• 제27권11호
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• pp.2028-2036
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• 2017

The Escherichia coli K-12 and B strains are among the most frequently used bacterial hosts for scientific research and biotechnological applications. However, omics analyses have revealed that E. coli K-12 and B exhibit notably different genotypic and phenotypic attributes, even though they were derived from the same ancestor. In a previous study, we identified a limited number of proteins from the two strains using two-dimensional gel electrophoresis and tandem mass spectrometry (MS/MS). In this study, an in-depth analysis of the physiological behavior of the E. coli K-12 and B strains at the proteomic level was performed using six-plex isobaric tandem mass tag-based quantitative MS. Additionally, the best lysis buffer for increasing the efficiency of protein extraction was selected from three tested buffers prior to the quantitative proteomic analysis. This study identifies the largest number of proteins in the two E. coli strains reported to date and is the first to show the dynamics of these proteins. Notable differences in proteins associated with key cellular properties, including some metabolic pathways, the biosynthesis and degradation of amino acids, membrane integrity, cellular tolerance, and motility, were found between the two representative strains. Compared with previous studies, these proteomic results provide a more holistic view of the overall state of E. coli cells based on a single proteomic study and reveal significant insights into why the two strains show distinct phenotypes. Additionally, the resulting data provide in-depth information that will help fine-tune processes in the future.

• 한국미생물·생명공학회지
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• 제24권4호
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• pp.472-477
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• 1996

Natural human epidermal growth factor (nhEGF) was purified from pregnant human urine by benzoic acid adsorption, DEAE-Sepharose ion exchange, and immunoaffinity chromatography. The purified nhEGF was further separated into four fractions using Bondapak C$_{18}$ HPLC system. Following characterization by Western blot analysis and double immu- nodiffusion, we found that each fraction corresponds to four derivatives of the nhEGF. For biological analysis of nhEGF, we optimized the labeling time and serum concentration for the incorporatioin of 5-bromo-2'-deoxy uridine (BrdU), a non-radioactive alternative for [$^{3}$H]-thymidine uptake, into NIH 3T3 cells. The DNA synthesis of NIH 3T3 cells was gradually increased at the nhEGF concentrations between 0.1 - 10 ng/ml in the Dulbecco's Modified Eagles Medium (DMEM) containing 0.2% Fetal calf serum (FCS). When we assayed the biological activity of four fractions, the activity of the second fraction was superior to that of the others. Based on the results from the HPLC analysis spiked with recombinant human epidermal growth factor (rhEGF) and amino acid sequencing, we concluded that the second fraction was nhEGF and the other three fractions were the derivatives of nhEGF. In addition, the proportion of nhEGF was approximately 46% is compared with that of the other three derivatives.