• 한국보건간호학회지
• /
• 제34권2호
• /
• pp.165-181
• /
• 2020

Purpose: This study is an integrative review of previous research conducted on family-school nurse partnership in school health care, as perceived by school nurses, children, and families. Method: The study was conducted according to the five stages of integrative review suggested by Whittemore and Knafl (2005), which involved problem identification, literature search, data evaluation, data analysis, and presentation of results. Suitable literature was found using portals such as PubMed, CINAHL, Embase, and Web of Science. Key words such as school nurses, family, parents, children, and partnership were used to narrow the search results. Studies published in peer-review journals between 2006-2018 were selected. The quality of studies was appraised using critical appraisal tools provided by the Joanna Briggs Institute (2020). Result: Thirteen studies (5 quantitative and 8 qualitative) were finalized for the final review process. After an extensive review, four key themes of school nurse-family partnership in school health care were identified: partnership components, partnership barriers, partnership facilitators, and strategies to promote partnership. Conclusion: Partnership and its components, barriers, and facilitators, along with strategies for its promotion should be considered in school health care. Further studies are needed on the perceptions held by parents and children regarding school health care partnership.

• Journal of Microbiology and Biotechnology
• /
• 제5권3호
• /
• pp.132-137
• /
• 1995

The lambda integrase (lnt) is believed to bind to several arm and core sites of attP DNA in order to facilitate intasome formation. We have done systematic mutagenic analysis on all 5 arm sites and found that P1 is absolutely required for integration while P2 is not. We also found that all 3 P' arm sites(P'1, P'2, and P'3) are required for efficient integrative recombination. P'1, which is an important binding site for excision, also seems to be crucial for integration when preincubation of attP DNA with Int and IHF is performed before recombination. Preincubation assay revealed that preincubation with Int and IHF improved the efficiency of recombination of wild type attP DNA and demolished recombinations of P'1 mutant attP DNAs.

• 한국식물생명공학회:학술대회논문집
• /
• 한국식물생명공학회 2005년도 춘계학술대회 및 국제심포지움 초록집
• /
• pp.66-66
• /
• 2005

• 한국데이타베이스학회:학술대회논문집
• /
• 한국데이타베이스학회 2000년도 추계학술대회 E-Business와 정보보안
• /
• pp.27-37
• /
• 2000

o An Enriched conceptualization of BM o Needs further research on: - An Integrative framework for BM research - A dynamic. evolutionary account of BM - Empirical validation of the analysis models - Draw practical implications and guidelines for business (omitted)

• Journal of Ginseng Research
• /
• 제47권1호
• /
• pp.123-132
• /
• 2023

Background: Pseudotyped virus systems that incorporate viral proteins have been widely employed for the rapid determination of the effectiveness and neutralizing activity of drug and vaccine candidates in biosafety level 2 facilities. We report an efficient method for producing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus with dual luciferase and fluorescent protein reporters. Moreover, using the established method, we also aimed to investigate whether Korean Red Ginseng (KRG), a valuable Korean herbal medicine, can attenuate infectivity of the pseudotyped virus. Methods: A pseudovirus of SARS-CoV-2 (SARS-2pv) was constructed and efficiently produced using lentivirus vector systems available in the public domain by the introduction of critical mutations in the cytoplasmic tail of the spike protein. KRG extract was dose-dependently treated to Calu-3 cells during SARS2-pv treatment to evaluate the protective activity against SARS-CoV-2. Results: The use of Calu-3 cells or the expression of angiotensin-converting enzyme 2 (ACE2) in HEK293T cells enabled SARS-2pv infection of host cells. Coexpression of transmembrane protease serine subtype 2 (TMPRSS2), which is the activator of spike protein, with ACE2 dramatically elevated luciferase activity, confirming the importance of the TMPRSS2-mediated pathway during SARS-CoV-2 entry. Our pseudovirus assay also revealed that KRG elicited resistance to SARS-CoV-2 infection in lung cells, suggesting its beneficial health effect. Conclusion: The method demonstrated the production of SARS-2pv for the analysis of vaccine or drug candidates. When KRG was assessed by the method, it protected host cells from coronavirus infection. Further studies will be followed for demonstrating this potential benefit.

• Natural Product Sciences
• /
• 제24권3호
• /
• pp.199-205
• /
• 2018

To determine the optimum extraction conditions that give the highest yield of isoquercitrin and caffeic acid from Aster scaber, the effects of four extraction variables (solvent concentrations, extraction time, number of repeated extraction, and solvent volumes) on isoquercitrin and caffeic acid yield was examined via HPLC-UV. Our results showed that the highest extract and isoquercitrin yield were observed when A. scaber was extracted with 450 mL distilled water for 8 hr repeatedly for three times. In case of caffeic acid, the content was higher in the two repeated extracts. Also, content analysis of isoquercitrin in Aster species was performed in which A. fastigiatus, A. ageratoides, and A. scaber exhibited the highest isoquercitrin content at 6.39, 5.68, and 2.79 mg/g extract, respectively. In case of caffeic acid, the highest content of A. scaber and A. glehni was 0.64 and 0.56 mg/g extract, respectively. This study reports an optimized method for extraction of isoquercitrin and caffeic acid from A. scaber and evaluates potential sources of the compounds.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권8호
• /
• pp.4031-4036
• /
• 2012

Background: The negative signaling provided by interactions of the co-inhibitory molecule, programmed death-1 (PD-1), and its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2), is a critical mechanism contributing to tumor evasion; blockade of this pathway has been proven to enhance cytotoxic activity and mediate antitumor therapy. Here we evaluated the anti-tumor efficacy of AAV-mediated delivery of the extracellular domain of murine PD-1 (sPD-1) to a tumor site. Material and Methods: An rAAV vector was constructed in which the expression of sPD-1, a known negative regulator of TCR signals, is driven by human cytomegalovirus immediate early promoter (CMV-P), using a triple plasmid transfection system. Tumor-bearing mice were then treated with the AAV/sPD1 construct and expression of sPD-1 in tumor tissues was determined by semi quantitative RT-PCR, and tumor weights and cytotoxic activity of splenocytes were measured. Results: Analysis of tumor homogenates revealed sPD-1 mRNA to be significantly overexpressed in rAAV/sPD-1 treated mice as compared with control levels. Its use for local gene therapy at the inoculation site of H22 hepatoma cells could inhibit tumor growth, also enhancing lysis of tumor cells by lymphocytes stimulated specifically with an antigen. In addition, PD-1 was also found expressed on the surfaces of activated CD8+ T cells. Conclusion: This study confirmed that expression of the soluble extracellular domain of PD-1 molecule could reduce tumor microenvironment inhibitory effects on T cells and enhance cytotoxicity. This suggests that it might be a potential target for development of therapies to augment T-cell responses in patients with malignancies.

• 한국학교보건학회지
• /
• 제23권2호
• /
• pp.143-150
• /
• 2010

Purpose: This study aims to examine what can be seen in children's paintings, their common characteristics, and what role art therapy plays in diagnosing the post-traumatic stress disorder in children who experienced trauma, through an art therapy program among elementary school students that witnessed the death of parents from a fire drill accident. Methods: A program of three times of art therapy was progressed among 34 children who witnessed a fire accident. Revised Children's Manifest Anxiety Scale (RCMAS) was used for comparative analysis of art therapy results. Results and Conclusion: First, children who had the possibility to develop post-traumatic stress disorder were found through art therapy. Second, an opportunity to express themselves were given to children who refused psychological tests or treatment through art therapy.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2012년도 춘계학술대회 논문집
• /
• pp.439-440
• /
• 2012

New analysis methods complement classical approaches in the vehicle NVH development by reducing and accelerating iteration steps to obtain a target sound. Therefore, tools are required that allow an integrative approach of sound engineering and structural analysis and enable a precise simulation and modification based on measured data. The Response Modification Analysis (RMA) is such a hybrid solution, which provides indications of relevant transfer paths taking into account the sensitivity of response channels to modifications of reference channels.

• 정보처리학회논문지:소프트웨어 및 데이터공학
• /
• 제2권1호
• /
• pp.7-18
• /
• 2013

최근 정보통신기술의 발전으로 교육훈련 현장에는 전자 교수학습 시스템(e-teaching and learning system) 에 대한 사용이 급증하고 있다. 또한 무선 이동통신기술과 멀티미디어 처리 기술의 발전에 따라 언제 어디서나 양질의 교육 및 훈련 콘텐츠에 쉽게 접근할 수 있게 되었고, 개인 맞춤형의 교수학습 서비스 시스템도 등장하고 있다. 많은 전자 교수학습 시스템들이 개발됨에 따라 이의 보안성(security) 역시 중요한 이슈로 떠오르고 있다. 전자 교수학습 시스템들과 같은 정보 서비스 시스템의 보안성을 평가하기 위한 여러 가지 결함 분석 기법들이 있다. 그러나 융합IT 기술이 확대 발전함에 따라 정보 서비스 시스템의 종류와 특성이 다양해지면서 단지 하나의 시스템 보안성 평가 방법으로는 적절한 분석이 이루어 질 수 없다. 따라서 본 논문에서는 정보 서비스 시스템의 보안성을 평가하기 위해서 결함 트리 분석(Fault Tree Analysis; FTA)기법과 고장모드별 영향 분석(Fault Modes and Effect Analysis; FMEA)기법의 두 가지의 분석 기법을 통합하여 사용함으로서 전자 교수학습 시스템의 결함 분석을 수행하고 보안성을 개선하는 방법을 제안한다.