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EXPRESSION OF MATRIX METALLOPROTEINASE-1 AND -2 MRNA IN RETRODISCAL TISSUE OF THE TEMPOROMANDIBULAR JOINT (측두하악 관절원판 후조직의 MMP(matrix metalloproteinase)-1과 MMP-2 mRNA의 발현)

  • Huh, Jong-Ki;Park, Kwang-Kyun;Choi, Min-Ah;Kim, Hyung-Gon
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.29 no.4
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    • pp.212-218
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    • 2003
  • Matrix metalloproteinases (MMPs) play an important role in the normal morphogenesis, maintenance, and repair of matrix and also have important functions in pathologic conditions characterized by excessive degradation of extracellular matrix, such as rheumatoid arthritis, osteoarthritis, periodontitis and in tumor invasion and metastasis. In this study, expression of MMP-1 and -2 mRNA in retrodiscal tissue of the temporomandibular joint (TMJ) was examined and compared with magnetic resonance imaging (MRI) and surgical findings. MMP mRNAs in the retrodiscal tissue samples were detected by reverse transcription - polymerase chain reaction. TMJ internal derangement (ID) was categorized as normal disc position, disc displacement with reduction, early stage of disc displacement without reduction (DDsR) and late stage of DDsR. TMJ osteoarthrosis (OA) was classified with normal, mild and advanced OA. The amount of synovial fluid collection was divided into not detected, small, large and extremely large amount on MR T2-weighted imaging. Perforation and adhesion were examined during open surgery of the TMJ. Six out of 37 samples were excluded because of little amount of extracted total mRNA. MMP-2 mRNA was detected whole joints, and so the MMP-2 mRNA seems to be expressed normally in retrodiscal tissue. However, MMP-1 mRNA was expressed in 8 of 31 joints. Frequencies of MMP-1 mRNA expression according to the TMJ IDs, amount of synovial fluid and surgical findings made no significant difference. MMP-1 mRNA was detected more frequently in OA groups (7/16 joints, 43.8%) than in normal bony structure group (1/15 joints, 6.7%). Expression of MMP-1 mRNA in retrodiscal tissue might be related with OA of the TMJ.

Demonstration of constant nitrogen and energy amounts in pig urine under acidic conditions at room temperature and determination of the minimum amount of hydrochloric acid required for nitrogen preservation in pig urine

  • Jongkeon Kim;Bokyung Hong;Myung Ja Lee;Beob Gyun Kim
    • Animal Bioscience
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    • v.36 no.3
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    • pp.492-497
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    • 2023
  • Objective: The objectives were to demonstrate that the nitrogen and energy in pig urine supplemented with hydrochloric acid (HCl) are not volatilized and to determine the minimum amount of HCl required for nitrogen preservation from pig urine. Methods: In Exp. 1, urine samples of 3.0 L each with 5 different nitrogen concentrations were divided into 2 groups: 1.5 L of urine added with i) 100 mL of distilled water or ii) 100 mL of 6 N HCl. The urine in open plastic containers was placed on a laboratory table at room temperature for 10 d. The weight, nitrogen concentration, and gross energy concentration of the urine samples were determined every 2 d. In Exp. 2, three urine samples with different nitrogen concentrations were added with different amounts of 6 N HCl to obtain varying pH values. All urine samples were placed on a laboratory table for 5 d followed by nitrogen analysis. Results: Nitrogen amounts in urine supplemented with distilled water decreased linearly with time, whereas those supplemented with 6 N HCl remained constant. Based on the linear broken-line analysis, nitrogen was not volatilized at a pH below 5.12 (standard error = 0.71 and p<0.01). In Exp. 3, an equation for determining the amount of 6 N HCl to preserve nitrogen in pig urine was developed: additional 6 N HCl (mL) to 100 mL of urine = 3.83×nitrogen in urine (g/100 mL)+0.71 with R2 = 0.96 and p<0.01. If 62.7 g/d of nitrogen is excreted, at least 240 mL of 6 N HCl should be added to the urine collection container. Conclusion: Nitrogen in pig urine is not volatilized at a pH below 5.12 at room temperature and the amount of 6 N HCl required for nitrogen preservation may be up to 240 mL per day for a 110-kg pig depending on urinary nitrogen excretion.

An Experimental Study to Predict the Concentration of Moving Tire and Road Wear Particles from Road to Ocean Environment (도로에서 해양 환경까지 이동하는 타이어 마모입자의 농도를 예측하기 위한 실험적 연구)

  • Tae-Woo Kang;Won-Hyun Ji
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.12 no.2
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    • pp.196-205
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    • 2024
  • In this study, sample collection and quantification analysis of Tire and Road Wear Particles (TRWP) from the road surface were conducted to predict the amount of TRWP generated on the road surface moving by environmental compartment depending on rainfall intensity. Samples were collected from TRWP remaining on the road surface two days after the 3 days average rainfall (0-60 mm/day) occurred and the road surface was completely dry. Only TRWP were separated from the collected samples through size and density separation, and the difference in the content of TRWP remaining on the road surface after rainfall was based on the value of 60.2 g o f TRWP o n a day witho ut rain (0 mm/day). By calculating, it was co nfirmed that 0-49.4 g o f TRWP can mo ve to the environmental compartment depending on the intensity of rainfall. In addition, it was confirmed that when the rainfall intensity was 60 mm/day, the amount of TRWP moving to each environmental section was 3.75 times higher compared to 5 mm/day, and using the results of previous research, the total amount of TRWP that can be transported to the environmental compartment by rainfall from the domestic road environment annually is 9,592 tons, and 288 tons of this can be affected by marine microplastics. However, this study has limitations in terms of limited space and predicted results, but it would like to mention the need to improve the domestic road environment and sewage treatment system to reduce TRWP. In the future, we plan to conduct sample collection and concentration analysis studies of TRWP in real environmental compartments to verify the results of this study.

The Case Study on the Application of the Standard Method for the Estimation of Range and Degree of Fisheries Damages Caused by Warm Water Drained by Generating Plants (발전소 온배수에 의한 어업피해범위와 정도 및 어업처분을 결정하는 표준화 방안의 적용사례분석)

  • Kang Yong-Joo;Kim Ki-Soo
    • The Journal of Fisheries Business Administration
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    • v.37 no.1 s.70
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    • pp.73-93
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    • 2006
  • This paper tries to apply the standard method for the estimation of range and degree of fisheries damages caused by warm water drained by generating plants to a real situation. The theoretic foundation of the study has been shown in the article published on the journal of fisheries business administration of last year. The paper tries to examine the degree of external appicability of the theoretic model through empirical study. The results of the study are as follows. The model has been found a successful device for the estimation of fisheries damages caused by warm water drained by generating plants. It also shows a possibility to work in any other fisheries damage cases caused by several kinds of public undertaking on coastal areas. The key of the model is the successful derivation of the quantity amount of the critical variation of environmental factors for example, in this case, water thermal variation. Therefore the countinous and appropriate collection of law data on the environmental factors under a viewpoint of statistics is essential to the usefulness of the model.

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Investigation the effect of dynamic loading on the deformation of ancient man-made underground spaces

  • Rezaee, Hooman;Noorian-Bidgoli, Majid
    • Earthquakes and Structures
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    • v.22 no.3
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    • pp.277-287
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    • 2022
  • The ancient underground cities are a collection of self-supporting spaces that have been manually excavated in the soil or rock in the past. Because these structures have a very high cultural value due to their age, the study of their stability under the influence of natural hazards, such as earthquakes, is very important. In this research, while introducing the underground city of Ouyi Nushabad located in the center of Iran as one of the largest man-made underground cities of the old world, the analysis of dynamic stability is performed. For this purpose, the dynamic stress-displacement analysis has been performed through numerical modeling using the finite element software PLAXIS. At this stage, by simulating the Khorgo earthquake as one of the large-scale earthquakes that occurred in Iran, with a magnitude of 6.9 on the Richter scale, dynamic analysis by time history method has been performed on three selected sections of underground spaces. This study shows that the maximum amount of horizontal and vertical dynamic displacement is 12.9 cm and 17.7 cm, respectively, which was obtained in section 2. The comparison of the results shows that by increasing the cross-sectional area of the excavation, especially the distance between the roof and the floor, in addition to increasing the amount of horizontal and vertical dynamic displacement, the obtained maximum acceleration is intensified compared to the mapping acceleration applied to the model floor. Therefore, preventive actions should be taken to stabilize the excavations in order to prevent damage caused by a possible earthquake.

METHODS TO IMPROVE UTILIZATION OF RICE STRAW II. EFFECTS OF DIFFERENT LEVELS OF FEEDING ON INTAKE AND DIGESTIBILITY OF UNTREATED AND UREA AMMONIA TREATED RICE STRAW

  • Badurdeen, A.L.;Ibrahim, M.N.M.;Schiere, J.B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.7 no.2
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    • pp.165-169
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    • 1994
  • Forty cross bred ($168\;{\pm}\;28\;kg$) bull calves were offered either untreated (US) or urea ammonia treated (TS) rice straw at five levels of feeding, namely; 60, 80, 100, 120 140% of ad libitum. Ad libitum level for each animal was estimated over a period of one month, which was followed by a preliminary period of 21 days and a collection period of 15 days. The maximum organic matter intake (OMI-kg/100 kg BW) were 2.08 and 3.35 for US and TS, respectively and urea treatment increased the maximum OMI by 61% than for US. In order to reach maximum intake the amount feed refusal should be 25% for US, but with TS maximum intake was not reached even when the amount of feed refused was 39% of that consumed. The OMD of US significantly decreased with increase in OMI, whereas with TS the decrease was not significant. The inability of animals to select between stems and leaves particularly in TS might be one of the reasons for its constant digestibility. Considering the substantial increases in intake and the negative effect on digestibility of US, further trials are warranted, as well as studies to determine the practical implications.

Differences in the expression rate and genotype of Porphyromonas gingivalis according to smoking status (흡연행태에 따른 Porphyromonas gingivalis의 발현율과 유전형 차이)

  • Kim, Jin-Kyoung
    • Journal of Korean Clinical Health Science
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    • v.8 no.2
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    • pp.1436-1443
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    • 2020
  • Purpose: The purpose of this study was to differences in the expression rate of Porphyromonas gingivalis according to smoking status, smoking amount and period of smoking. Methods: At the time of investigation, 30 smokers and non-smokers were recruited among patients with periodontitis with a probing pocket depth(PPD) of 4 mm or more. General information was collected using a self-questionnaire, and the average value was used by a dentist to measure the probing pocket depth of three times each for the first or second molar. Plaque collection and analysis were performed by collecting only subgingival plaque using a conventional method, and the expression rate of P. gingivalis was confirmed using polymerase chain reaction (PCR). For statistical analysis, the SPSS Ver 25.0 program was used. Results: Smoking did not have a significant effect on the expression of P. gingivalis, but it did affect the expression of more type II genotypes (p<0.05). In addition, smokers had more slight periodontal pocket, and the amount and duration of smoking did not affect the expression of P. gingivalis. Conclusions: In the future, it is necessary to reinforce the group of smokers and non-smokers with healthy oral conditions, and to investigate the quantitative difference in the expression rate and genotype of P. gingivalis over time of harmful substances in smoking.

Efficient K-Anonymization Implementation with Apache Spark

  • Kim, Tae-Su;Kim, Jong Wook
    • Journal of the Korea Society of Computer and Information
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    • v.23 no.11
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    • pp.17-24
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    • 2018
  • Today, we are living in the era of data and information. With the advent of Internet of Things (IoT), the popularity of social networking sites, and the development of mobile devices, a large amount of data is being produced in diverse areas. The collection of such data generated in various area is called big data. As the importance of big data grows, there has been a growing need to share big data containing information regarding an individual entity. As big data contains sensitive information about individuals, directly releasing it for public use may violate existing privacy requirements. Thus, privacy-preserving data publishing (PPDP) has been actively studied to share big data containing personal information for public use, while preserving the privacy of the individual. K-anonymity, which is the most popular method in the area of PPDP, transforms each record in a table such that at least k records have the same values for the given quasi-identifier attributes, and thus each record is indistinguishable from other records in the same class. As the size of big data continuously getting larger, there is a growing demand for the method which can efficiently anonymize vast amount of dta. Thus, in this paper, we develop an efficient k-anonymity method by using Spark distributed framework. Experimental results show that, through the developed method, significant gains in processing time can be achieved.

Behavior-induced Disposable Cup Automatic Separation X-Bin Study

  • LEE, Su-Min;KIM, Seo-Hyun;KIM, Ye-Na;BYUN, Hyo-Jeong;KWON, Lee-Seung;JUNG, Min-Jae;KWON, Woo-Taeg
    • Journal of Wellbeing Management and Applied Psychology
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    • v.4 no.1
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    • pp.19-25
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    • 2021
  • Purpose: This study seeks to develop behavior-induced automatic separation X-Bin for disposable cup speculators. Some students throw away the contents. Research design, data and methodology: Therefore, environmental beautification workers spend a lot of time sorting disposable cups at school garbage collection sites. Disposable cups that have been thrown away like this are bad in terms of appearance and smell. In addition, cleaning inside garbage affects recycling rates and is closely related to environmental problems. Results: Clean cleaning reduces the amount of garbage incinerated without being recycled and reduces the cost of recycling. According to Korean Social Trends 2020, COVID-19 began to spread, and recycling of disposable items such as disposable cups increased by about 15 percent from the same period last year. Conclusions: These are classified for recycling, and the amount of garbage thrown on the streets is estimated to be larger. In order to prevent COVID-19s, the use of disposable goods is inevitable, but facilities are needed to dispose of them properly.

A Study of the Presence of Carbonic Acid and Other Potentially Hazardous Substances in Cheongsong Mineral Water (청송약수의 탄산과 유해 가능성 물질 존재에 관한 연구)

  • Lee, Sung-Ho
    • The Korean Journal of Food And Nutrition
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    • v.34 no.1
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    • pp.132-136
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    • 2021
  • The purpose of this study is to measure the levels of eluted and dissolved CO2, and CO, volatile organic substances and radiation composition of Cheongsong mineral water which were collected from November 2019 to July 2020 during the autumn, spring, and summer seasons at collection points located in the upper, middle and lower spring waters. Data of the upper, middle and lower spring waters include the following: the amount of eluted water (average value±standard deviation, mL/min) was 30.07±0.52, 15.03±0.16, 23.73±0.42, and the amount of CO2 gas was 1,000 ppm or more. In addition, there was no detection of CO or total volatile organic substances (TVOC) and the radiation dose was 0.08 to 0.13. μSv/h. A blank test value of 0.08 to 0.10 μSv/h, when compared with the median value, showed a high value of 0.02 μSv/h, and the uranium test results provided by the Cheongsong-gun Office were 0.0118 mg/L (date 2019.06.18) and 0.0091 mg/L (date 2020.06.04.) respectively, which was less than the permission limit of 0.03 mg/L. However, it is believed that further research using more precise devices is needed in order to guarantee the safety and health of the water.