• Analytical Science and Technology
• /
• v.16 no.6
• /
• pp.483-487
• /
• 2003

Amitrole is well-known as a non-selective herbicide and it is able to cause contamination of driking water as well as pollution of ground water and surface water. However, it is difficult to extract from water because it has a high solubility for water whereas a low solubility for general organic solvents. This method is described for the determination of amitrole in water samples by GC/MS. After evaporation of 10 mL water sample by a vacuum evaporator, amitrole was derivatized with isobutyl chloroformate (iso-BCF) on room temperature for 15~20 min. As a result, the sensitivity for GCfMS was improved as N-isobutoxycarbonyl amitrole derivative was formed. The linearity of the calibration curve showed good as 0.997. The recoveries were obtained more than 94.9% and relative standard deviations were less than 2.8% at $1.0{\mu}g/L$, $10.0{\mu}g/L$ and $100.0{\mu}g/L$. The limit of detection showed $0.1{\mu}g/L$ with a signal-to-noise ratio (S/N) of 3.

• Analytical Science and Technology
• /
• v.17 no.2
• /
• pp.117-129
• /
• 2004

Amitrole in environment, difficult to be analyzed by GC or GC/MS due to high polarity and low volatility, was analyzed by LC/ESI/MS in the study. Maximum peak intensity of amitrole in LC/MS/ESI mass spectrum is m/z 85 of protonated molecular ion $(M+H)^+$ with 30V of cone voltage at SIR mode. It was confirmed that ratios between main ion of amitrole, 85 of protonated molecular ion, and m/z 58 fragmented ion of amitrole, had increased corresponding with the increment of cone voltage from 20V to 70V. The isotope molecular weight of amitrole was $86([M+H])^+$ at LC/MS analysis and the mass spectrum ratio between 85 mass and 86 mass was not different by the change of concentration but similar to theoretical ratio(less than 10% standard deviation).The linearity of standard calibration curve under same condition with sample treatment method had $y=1.09354e^6X+26947.2$ and $r^2=0.99$. Recovery rates in water and soil samples were 77.64~83.44% and 71.11~79.44%, respectively. Reliability of the analysis was performed with 5 repeated measurements at each level of standard concentration and the result showed that relative standard deviation was less than 10%; therefore, the extraction and analysis method in the study suggested that it would be reliable to measure amitrole in water and soil media.

• Analytical Science and Technology
• /
• v.15 no.2
• /
• pp.149-156
• /
• 2002

In this study, the levels of DEHA, carbaryl, and amitrole contained in the fresh water fishes and amphibians were determined. The 31 sampling sites were selected including 29 sites along the several rivers and 2 sites from the well-known wet lands. The rivers consists of the Han, Nakdong, Kum, Youngsan, and other small scaled rivers. DEHA was detected from 10 sites, but carbaryl and amitrole were not detected at any sites. The highest average concentration of DEHA was determined from the Kum River by $52.7{\mu}g/kg$. In the detected 10 sites, the average concentration of DEHA from fishes was $40.0{\mu}g/kg$, and that from amphibians was $8.90{\mu}g/kg$ which indicates that 5 times more DEHA was observed from fishes than amphibians. The detection frequency from fishes was 10/62 while that from amphibians was 2/62, and the highest concentration from fishes was $95.5{\mu}g/kg$ (crucians from Kongjoo) and that from amphibians was $12.1{\mu}g/kg$ (bullfrog from Najoo). Among the 10 detected sites, 6 sites were agricultural area and the other 4 sites were semi-urban area.

• Korean Journal of Weed Science
• /
• v.16 no.4
• /
• pp.309-316
• /
• 1996

In this study, germination characteristics and herbicidal response of green kyllinga(Kyllinga brevifolia var. leiolepsis H.) were investigated. The storage method desirable for a rapid dormancy release was to keep the seed under low temp. and wetting condition for one to two months, or high temp($40^{\circ}C$) and drying condition for three months. The dormancy of rhizome was hardly observed. The optimum temperature for germination of seed and rhizome was around $30^{\circ}C$ and 16-$20^{\circ}C$, repectively. The germination of dormancy-breaked seed was completely dependent on light. Shoot emergence ratio(%) was decreased with increase of planting depth ; for example, only 18% of rhizome segments planted in the depth of 4cm under soil surface emerged above soil surface. Flooding at earlier growth stage resulted in significant decrease in shoot emergence as well as in dry weight. The germinablity of rhizome was almost lost as a decreased in fresh weight reached to 50%. Usually, green kyllinga was sensitive to herbicides such as bentazone, bensulfuron and benfuresate etc. which were known to be effective in Cyperaceae weeds, indicating that green kyllinga can be used as a representative plant in the screening of herbicides for Cyperus weeds.