• Title/Summary/Keyword: Amino-

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Biochemical Characteristics of Cd-binding High Molecular Weight Proteins (카드뮴 결합 고분자량 단백질의 생화학적 특성)

  • 천기정;김봉희
    • YAKHAK HOEJI
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    • v.39 no.4
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    • pp.380-384
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    • 1995
  • The isoelelectric points of Cd-BP(l) and Cd-BP(II), cadmium-binding proteins, were 6.01 and 5.35, respectively. Both of them contained zinc. As for the amino acid composition, Cd-BP(I) contained a lot of glycine and lysine but none of such aromatic amino acids as tyrosine and phenylalanine.. On the other hand, Cd-BP(II) contained leucine, histidine, asparti cacid and alanine but no aromatic amino acids.

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Qualitative Analysis of the Free Amino Acids in Gallula tornieri Tissues (맹꽁이 조직내 유리 아미노산의 정성적 분석)

  • 하두봉;이양림
    • The Korean Journal of Zoology
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    • v.4 no.2
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    • pp.7-12
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    • 1961
  • The present work deals with the patterns of the free amino acids in several tissues of Gallula tornieri VOGT analysed by means of paper chromatography and ion-exchange resins. The tissues used were heart, stomach , muscle, brain , testis , skin , and liver. 14 amino acid was detected in heart, 11 in muscle , 15 in stomach , 9 , in brain , 10 in skin in liver , and 10 in testis. It was found that there were distinguishable differences in the patterns of the free amino acids among the different tissues.

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Analysis on the Amino Acid Distributions with Position in Transmembrane Proteins

  • Chi, Sang-Mun
    • Journal of the Korean Data and Information Science Society
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    • v.16 no.4
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    • pp.745-758
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    • 2005
  • This paper presents a statistical analysis on the position-specific distributions of amino acid residues in transmembrane proteins. A hidden Markov model segments membrane proteins to produce segmented regions of homogeneous statistical property from variable-length amino acids sequences. These segmented residues are analyzed by using chi-square statistic and relative-entropy in order to find position-specific amino acids. This analysis showed that isoleucine and valine concentrated on the center of membrane-spanning regions, tryptophan, tyrosine and positive residues were found frequently near both ends of membrane.

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Lipid and Protein Constituents of Crotalaria juncea L.

  • Javed, Muhammad Akhtar;Saleem, Muhammad;Yamin, Muhammad;Chaudri, Tanvir Ahmad
    • Natural Product Sciences
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    • v.5 no.3
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    • pp.148-150
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    • 1999
  • Seed lipids and proteins of Crotalaria juncea L were analyzed for fatty acids and amino acids respectively. Gas chromatographic analysis of the oil gave palmitic acid (16.01%), stearic acid (7.29%), oleic acid (14.41%), linoleic acid (54.44%) and linolenic acid (7.86%). The defatted seed cake contained all the essential amino acids except methionine and six non-essential amino acids.

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Thin Layer Chromatogram by an Extracellular ${\beta}$-Amylase of Bacillus sp. KYJ 963 and its Amino Acid Composition

  • Kim, Young-Jae
    • Journal of Life Science
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    • v.11 no.2
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    • pp.92-93
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    • 2001
  • Bacillus sp. KYJ 963, which was isolated from Korean salt-fermented anchovy (anchovy-jeot), produces an extracellular ${\beta}$-amylase. The analysis of the digestion products of substrates by thin layer chromatography from the purified protein revealed that the enzyme could not hydrolyze maltose or ${\alpha}$-cyclodextrin. In the amino acid composition analysis, the major characteristic of the ${\beta}$-amylase was the high proportion of amino acids that possess short side chain such as glycine and alanine.

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Enantioselective Synthesis of Cyclic Amino Alcohols: cis-1-Amino-2-indanol

  • Kim, Eun-Jin;An, Kyung-Mi;Ko, Soo Y.
    • Bulletin of the Korean Chemical Society
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    • v.27 no.12
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    • pp.2019-2022
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    • 2006
  • (1S,2R)-cis-1-Amino-2-indanol has been synthesized. A unique feature of the synthesis involves securing the functionalities and the configurations of the two stereocenters on an acyclic precursor before cyclizing it into the final ring-skeleton. The strategy allows both the stereocenters to be controlled in an absolute manner.

Syntheses and Central Nervous Depressant Activity of Piperine Derivatives(III) N-Piperoyl Amino Acids (Piperine유도체의 합성 및 중추억제작용에 관한 연구(III) N-Piperoylamino Acid 유도체)

  • 이한구;임중기;이은방;우원식
    • YAKHAK HOEJI
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    • v.27 no.4
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    • pp.289-293
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    • 1983
  • Twelve new N-piperoyl amino acids were synthesized by acylation of amino acids with piperoylchloride in order to develop the new central nervous depressants. Silylation of the carboxylic group of amino acids not only precluded side reactions but-also facilitated acylation at room temperature without catalysts. The acute toxicity of all the synthesized compounds were lower than that of piperine itself, whereas they showed strong protective effect against the strychnine-induced mortality.

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Determination of Active Site in PRD1 DNA Polymerase by Site-specific Mutagenesis (Site-specific Mutagenesis에 의한 PRD1 DNA Polymerase의 활성부위 결정)

  • 황정원;정구홍
    • Korean Journal of Microbiology
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    • v.29 no.4
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    • pp.209-214
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    • 1991
  • The PRD1 DNA polymerase is a small multi-functional enzyme containing conserved amino acid sequences shared by family B DNA polymerases. Thus the PRD1 DNA polymerase provides an useful model system with which to study structure-functional relationships of DNA polymerase molecules. In order to investigate the functional and structural roles of the highly conserved amino acid sequences, we have introduced three mutations into a conserved amino acid of the PRD1 DNA polymerase. Genetic complememtation study indicated that each mutation inactivated DNA polymerase catalytic activity.

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The Interaction of Chiral Amino Thiols with Organozinc Reagents and Aldehydes: A Mechanism of Amino Thiol-Catalyzed Addition of Organozinc Reagents to Aldehydes

  • 강자효;김진범;김지영;이덕환
    • Bulletin of the Korean Chemical Society
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    • v.19 no.4
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    • pp.475-481
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    • 1998
  • Details of various equilibria involved in the reactions of oxaza- and thiazazincolidine catalysts, generated from either β-amino alcohol or β-amino thiol, with aldehyde were studied by colligative measurements. The results indicated that the coordination of diethylzinc prior to the coordination of aldehyde is essential for high enantioselectivity of the thiol catalyzed reaction. The probable origin of asymmetric nonlinearity is also presented.