• 한국동물위생학회지
• /
• 제40권1호
• /
• pp.27-33
• /
• 2017

Salmonella enterica subsp. enterica is a common microbial flora in pet turtles, which could opportunistically become pathogenic to human. Their possession of aminoglycoside resistance genes has important significance both in humans and animal medicine. In this study, twenty-one Salmonella enterica subsp. enterica were isolated from thirty-five individual turtles purchased from pet shops and online markets in Korea. In order to characterize the aminoglycoside susceptibility patterns, antimicrobial susceptibility tests were performed against gentamicin, amikacin and kanamycin of aminoglycoside antimicrobial group. Each of the isolates showed susceptibility to all tested aminoglycosides in disk diffusion and minimum inhibitory concentration (MIC) tests. PCR assay was carried out to determine aminoglycoside resistance genes, integron and integron mediated aminoglycoside genes. None of the isolates showed aac(3)-IIa, aac-(6')-Ib, armA, aphAI-IAB aminoglycoside resistance genes. Only, five isolates (24%) harbored class 1 integron related IntI1 integrase gene. The results suggest that Salmonella enterica subsp. enterica strains isolated from pet turtles are less resistance to aminoglycosides and don't harbor any aminoglycosides resistance genes.

• Archives of Plastic Surgery
• /
• 제33권3호
• /
• pp.330-334
• /
• 2006

Polymicrobial nature of diabetic foot infection has been well documented in the literature. Initial antibiotic therapy of diabetic foot infection is usually empiric until reliable culture data is shown. This study was carried out to determine the common bacteriological flora of diabetic foot infection and antimicrobial sensitivity pattern in order to enhance possible empiric treatment. The specimens were obtained from wounds of 207 cases of diabetic foot ulcer, and the bacteriological isolation, and antimicrobial susceptibility tests of the isolates were carried out by standard microbiological methods. Staphylococcus aureus was the most common isolate, with 46.2% of recover rate among total bacterial isolated cases. Among gram-negative organisms, Pseudomonas aeruginosa was most common. Gram-positive organisms showed significant susceptibility to clindamycin, trimethoprim/sulfamethoxazole, and levofloxacin, besides vancomycin. Cefoperazone, piperacillin/tazobactam, and amikacin in addition to imipenem were most effective agents compared to gram-negative organisms. Diabetic foot infection requires use of combined antimicrobial therapy for initial management. Our results indicate that the most effective antibiotic combination for diabetic foot infection of Korean patients is clindamycin plus cefoperazone.

• 한국동물위생학회지
• /
• 제30권1호
• /
• pp.1-12
• /
• 2007

A liquid chromatographic method has been developed for the analysis of aminoglycoside antibiotics (AMGs) using Heptafluorobutyric acid (HFBA) as a ion-pairing reagent. AMGs (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygrosin B, kanamycin, neomycin, spectinomycin and tobramycin) were formed by reaction with HFBA as ion-pairing reagent. HFBA was attached to corresponding amino group of AMGs. These AMGs compounds were separated and detected by electrospray ionization mass spectrometry (ESI-MS). The experimental conditions for separation of AMGs were optimized and validated. A simple liquid chromatographic method for the determination of AMGs was demonstrated.

• 대한수의학회지
• /
• 제31권4호
• /
• pp.449-456
• /
• 1991

The present study was conducted to investigate biochemical properties and antimicrobial drug susceptibilities of 57 strains of Pasteurella haemolytica (P haemolytica) isolated from pneumonic calves and goats in Youngnam province during the period from September 1989 to February 1991. P haemolytica was isolated from 95 of 142(31.7%) pneumonic calves of 1 to 6 months of age and from 9 of 24(37.5%) pneumonic goats of 2 to 10 months of age. Seasonal isolation frequency of P haemolytica in calves and goats varied from 16.6 to 35.7%, and it was higher in winter. The majority of biochemical and cultural properties of P haemolytica isolated from calves and goats were identical to those of the reference strains employed. All isolates were susceptible to ceftiofur, erythromycin, kanamycin, gentamicin, and chloramphenicol (MIC: <$25{\mu}g/ml$), some of them were resistant to amikacin, sulfadimethoxin, and streptomycin (MIC: >200IU or ${\mu}g/ml$).

• 대한수의학회지
• /
• 제29권4호
• /
• pp.487-492
• /
• 1989

The capsular serogroupes and drug susceptibility of 111 isolates of Pasteurella multocida from pigs with atrophic rhinitis and pneumonic lesions were investigated. Of the 111 P multocida isolates, 42 were from lung lesions, 47 from nasal turbinate lesions and the remaining 22 from the nasal swabs. P multocida isolates were typed for capsular serogroupes A by hyaluronidase inhibition of capsule and D by acriflavine auto-agglutination. Most isolates(64.9%) were type A, 23.4% were type D and the remaining 11.7% were untypable. Resistance to triple sulfa(97.3%) was most frequent, followed by resistance to tiamulin(71.2%), tylosin(56.8%), streptomycin(36.9%), and neomycin(36.0%). The majority of the organisms were susceptible in order of prevalence to baytril(100%), ampicillin(98.2%), linsmycin(97.3%), colistin(97.3%), cephalothin(94.6%), gentamicin(93.7%), amikacin(92.3%), tetracycline(91.9%), trimethoprim/sulfamethoxazole(91.0%), and kanamycin(90.1%). No differences in drug resistance in relation to capsular serogroupes of P multocida and the origin of lesions were noted. A high prevalence of multiple drug resistance was observed and the most common resistant patterns were Sss, Tm, Ty(12.6%) and Sm, Sss, Tm, Ty(8.1%) patterns.

• 대한수의학회지
• /
• 제50권3호
• /
• pp.231-237
• /
• 2010

This study was conducted to investigate characteristics and antimicrobial susceptibility of Escherichia (E.) coli isolates isolated from vaginal mucosa and clitorial fossa of 105 Thoroughbred mares suspicious of the genital disease in Korea during the period from March 2006 to July 2007. Ninety six E. coli isolates were identified as standard biochemical properties and using BIOLOG system. Fifty three isolates (55.2%) could be classified into a total of 21 O serotypes and forty three isolates (44.8%) were non-typeable with 51 O antisera used in this study. The verotoxin 1 (VT 1) and verotoxin 2 genes were analyzed by multiplex PCR. Among them, one isolate was detected VT 1 gene (130 bp). Most of isolates showed a high susceptibility in ciprofloxacin (100%), enrofloxacin (100%), norfloxacin (100%), cefoxitin (96.9%), gentamicin (96.9%), sulphamethoxazole (96.9%), nitrofurantoin (94.8%), amikacin (93.8%), nalidixic acid (92.7%) and tetracycline (90.6%). These results may provide the basic information to establish strategies for the treatment and prevention of reproductive disease in Thoroughbred mares in Korea.

• 대한미생물학회지
• /
• 제16권1호
• /
• pp.29-34
• /
• 1981

Species and antimicrobial susceptibility of nonfermenting gram-negative bacilli(NFB) isolated from clinical materials at Yonsei Medical Center during the period of September 1980 to August 1981 were analyzed and the following results were obtained. 1. NFB were isolated from 17.1% of sputum, 14.8% of pus, 5.0% of urine, 3.3% of throat and 1.4% of blood specimens. 2. Among the isolates 57.6% were Pseudomonas aeruginosa and 32.7% were Acinetobacter calcoaceticus. P. maltophilia and P. cepacia were 3.2% and 2.8% respectively. Other bacteria were rarely isolated. 3. The monthly isolation rates were high during the June to November period(8.8-12.0%), and low during the December to May period(4.2-8.4%). 4. Many of the isolates showed resistance to various antimicrobials. Although there were variations depending on the bacterial species, amikacin, colistin, gentamicin, tobramycin and co-trimoxazole showed more antibacterial activities than other antimicrobials.

• 한국식품영양학회지
• /
• 제10권2호
• /
• pp.166-173
• /
• 1997

1993년부터 1995년까지 약 3개년 동안 우리 나라에서 사육하고 있는 가축의 유병율을 조사한 결과, 닭 16,200수 중에서 54수(0.3%)가 괴사성 장염에 이환 되었으며 새끼돼지 620두 중에서 9두(13.8%)가 장독혈증에 이환 되었다. C. perfringens의 분리율을 조사한 결과, 괴사성 장염에 이환 되어있는 닭 54수 중 7수(13.0%)에서 본균이 분리되었으며 장독혈증에 이환 되어있는 새끼돼지 66두 중 14두(21.2%)에서 본균이 분리되었고 장독혈증에 이환 되어있는 소 9두 중 3두(33.3%)에서 본균이 분리되었다. Acriflavine을 이용하여 C. perfringens의 S-R변이를 조사한 결과, S-R변이는 mercuric chloride, nicotine, caffeine, cysteine, glucose 등의 순으로 나타났는데, 이중 mercuric chloride가 가장 감수성이 높았다. C. perfringens의 항균요법제에 대한 감수성은 cepalothin, penicillin, erythromycin, amikacin 등이 높은 감수성을 나타내었는데, S-R변이 후에는 감수성이 일반적으로 저하되는 경향이었다. 중화시험법을 이용하여 C. perfringens의 독소형을 분류한 결과, cd 24균주 중 22균주(91.7%)는 A형이었으며 2균주(8.3%)는 C형이었다.

• Journal of Periodontal and Implant Science
• /
• 제26권1호
• /
• pp.202-215
• /
• 1996

The purpose of this study was to an in estigate the antibiotic susceptibiliity of Actinoobacillus actinomycetemcomitans isolated from the subgingival plaque to adult periodontitis. Seven bacterial strains were tested for their susceptibility to 10 antimicrobial agents under disk diffusion method and broth dilution methold, Seven patients with deep pocket(6mm) were selected for this study. They had not taken antibiotics for 6 months and no history of dental treatment for 6 months and no history of dental treatment for 6 months before this study. The results were as follows : 1. For the antibiotic disk diffusion method, seven A. actinimycetemcomitans were tested with 10 antimicrobial agents which comprised penicillin, gentamycin, clindamycin, lincomycin, ampicillin, erythromycin, tetracycline, amikacin, chloramphenicol, and vancomycin. The sensitive antibiotics were tetracycline, vancomycin, and chloramphenicol and the resistant antibiotics were clindamycin, and lincomycin. The other antimicrobial agents were less active. 2. From the study of determination on the minimal inhibitory concentration(MIC) by broth dilution method, the MIC of tetracycline to seven strains of the A. actinomycetemcomitans was $0.5-1.0{\mu}g/ml$, that of clindamycin was $32{\mu}g/ml$. These data suggest that tetracycline may be valuable drugs in the elimination of A. actinimycetemcomitans from the patients with adult periodontitis

• Clinical and Experimental Pediatrics
• /
• 제56권9호
• /
• pp.407-410
• /
• 2013

Klebsiella pneumoniae has emerged as a leading pathogen that causes pyogenic liver abscesses (PLAs) in Korea. K. pneumoniae liver abscess (KLA) is potentially life threatening, and the diagnosis is difficult. In developed countries, PLA is rarely observed in children and is frequently associated with disorders of granulocyte function and previous abdominal infection. We observed a case of KLA in a healthy 12-year-old boy. To our knowledge, this is the first reported case of KLA in an immunocompetent child without an underlying disease in Korea. The patient was treated with percutaneous catheter drainage and antibiotics. The catheter was placed in the intrahepatic abscess for 3 weeks and parenteral antibiotics (ceftriaxone and amikacin) were administered for 4 weeks, followed by oral antibiotics (cefixime) for 2 weeks. We reported this case to raise awareness of KLA in immunocompetent children among physicians, and to review the diagnosis, risk factors, potential complications, and appropriate treatment of KLA.