• Journal of Korea Technology Innovation Society
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• v.2 no.2
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• pp.290-308
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• 1999

In this study a system for the treatment or recyling of organic wastes from both urban and rural area was recommended. It was developed based on the resource recovery system regarding human being by four tectnologies; forage, methane production, high-grade composting and complete decomposition. High quality compost can be produced by combining several kind of wastes produced from urban and agricultural areas. High quality compost must possess not only general characteristics of ordinary compost, but also a superior ability to improve the soil properties and must contain more nutrients for plant. Cedar chips were recommended as the main bulking agent to adjust moisture contents and air permeability. Charcoal and zeolite can be used not only as the second bulking agent but also as fertilizer for improve the soil amendment. Complete decomposition of organic wastes is defined by organic matter being completely converted to $CO_2$ and water. All the input water was evaporated by the heat produced through the oxidation of organic matter, In the present study, the complete treatments were successfully achieved for Shochu wastewater, swine wastes, thickened excess sewage sludge, wastes produced by Chinese restaurant and anaerobic digested sludge. First of all, recycling center of organic wastes should be established for the protect the environments and effective recovery of organic resources. This may means the way to derive the recovery of human value.

• Microbiology and Biotechnology Letters
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• v.23 no.2
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• pp.236-242
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• 1995

Biological control of soilborne plant pathogens by the addition of antagonistic microorganisms to the soil may offer a practical supplement or alternative to existing disease management strategies that depend heavily on chemical pesticides. Soil amendment with antagonistic microbes was non-effective because of high cost, low efficacy, and inconvenient usage on the treatment course. Therefore, seed coating formulation for the application of biological seed treatments has been being to apply successful disease suppression for many important crops. The objectives of this study were to investigate the optimal condition for the spore production of biocontrol agent Bacillus subtilis YBL-7 and the liquid coating formulation that contained a suspension of a proper aqueous binder, as well as a ground fine solid particulate material. The maximum yield has been obtained from 60 hrs-old culture at 30$\circ$C in spore forming (SF) medium containing 0.8% nutrient broth, 0.05% yeast extract, 10$^{-1}$ M MgCl$^{2}$, 10$^{-4}$ M MnCl$^{2}$, 10$^{-5}$ M dipicolinic acid, and pH 6.5. The optimal condition of dried spore preparation was achieved when cells of B. subtilis YBL-7 was heat-dried with 50$\circ$C for 2 hrs.

• Journal of Soil and Groundwater Environment
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• v.24 no.2
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• pp.1-7
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• 2019

The feasibility of using hydrated lime ($Ca(OH)_2$) was assessed in reducing soil loss in sloped land under field condition. During 6-month monitoring from May to October, amendment of hydrated lime (3%, w/w) to a test plot decreased soil loss by 76% as compared to the unamended plot. However, the growth of natural vegetation was hampered by hydrated lime addition due to pH increase. Hydrated lime can be used as an effective agent to prevent soil loss in sloped land, but additional treatments are needed to preserve vegetation growth, especially in crop fields.

• Journal of Soil and Groundwater Environment
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• v.22 no.1
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• pp.18-26
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• 2017

In a previous study, we assessed the feasibility of ferric chloride ($FeCl_3$) as a washing agent in bench-scale in-situ soil washing to remove Pb from agricultural paddy soil. Herein is a subsequent study to evaluate variations in soil properties after $FeCl_3$ soil washing in terms of fractionation and bioavailability of Pb and chemical properties of the soil. After soil washing, the soil pH decreased from 4.8 to 2.6 and the exchangeable fractions of Pb in the soil increased from 12 mg/kg to 15 mg/kg. Variations in the Pb fractionation of the soil increased Pb bioavailability by almost three-fold; however,the base saturation decreased by 75%. The concentrations of total nitrogen and available phosphate were similar before and after soil washing. The available silicate concentration significantly increased after soil washing but was two times lower than that of soil washed with HCl, which is widely used as a washing agent. This indicates that $FeCl_3$ can be an acceptable washing agent that protects the soil clay structure. The results suggest that soil amendment, such as liming, is needed to recover soil pH, reduce mobility of Pb, and provide exchangeable bases of Ca, Mg, and K as essential elements for the healthy growth of rice plants in reused soil that has been washed.

• The Plant Pathology Journal
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• v.22 no.4
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• pp.364-368
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• 2006

Survival of biocontrol agents and their effective colonization of rhizhosphere are the essential components for successful disease suppression. The effects of hydrogel supplement on bacterial survival and disease control were evaluated in pot and in the field. Addition of 2% hydrogel material to potting soil resulted in significant enhancement of colonization of biocontrol agent Serratia plymuthica A21-4 both in soil and rhizosphere of pepper plants. Rhizosphere colonization of S. plymuthica A21-4 retrieved from 40 days old pepper seedlings indicated 100 times higher bacterial population in hydrogel treated soil than in ordinary pot soil. The pepper plants sown in hydrogelated potting soil showed higher seed germination rate and the better growth of pepper plant than those in ordinary commercial pot soil. Although the suppression of Phytophthora capsid density in the potting soil by treatment of biocontrol agent A21-4 was not significantly different between in hydrogelated soil and ordinary potting soil, the suppression of Phytophthora blight between two treatments was significantly different. A21-4 treatment in hydrogelated potting soil was completely disease-free while same treatment in ordinary potting soil revealed 36% disease incidence. Our field study under natural disease occurrence also showed significantly less disease incidence(12.3%) in the A21-4 treatment in the hydrogelated soil compared to other treatments. Yield promotion of pepper by the A21-4 treatment in the hydrogelated potting soil was also recognized. Our results indicated that hydrogel amendment with biocontrol agent in pot soil would be a good alternative to protect pepper seedlings and increase plant yield.

• Asian Journal of Atmospheric Environment
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• v.2 no.2
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• pp.102-108
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• 2008

In China, energy and environmental problems are becoming serious owing to rapid economic development. Coal is the most problematic energy source because it causes indoor and outdoor air pollution, acid rain, and global warming. One type of clean coal technology that has been developed is the coal-biomass briquette (or bio-briquette, BB) technique. BBs, which are produced from pulverized coal, biomass (typically, agricultural waste), and a sulfur fixation agent (slaked lime, $Ca(OH)_2$) under high pressure without any binder, have a high sulfur-fixation effect. In addition, BB combustion ash, that is, the waste material, can be used as a neutralization agent for acidic soil because of its high alkalinity, which originates from the added slaked lime. In this study, we evaluated the suitability of alternative biomass sources, namely, aquatic plants, as a BB constituent from the perspective of their use as a source of energy. We selected three types of aquatic plants for use in BB preparation and compared the fuel, handling, and environmental characteristics of the new BBs with those of conventional BBs. Our results showed that air-dried aquatic plants had a higher calorific value, which was in proportion to their carbon content, than agricultural waste biomass; the compressive strength of the new BBs, which depends on the lignin content of the biomass, was high enough to bear long-range intracontinental transport in China; and the new BBs had the same emission control capacity as the conventional BBs.

• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1537-1543
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• 2008

We tested Trichoderma harzianum as a biocontrol agent for Rhizoctonia solani AG2-1, using six natural antifungal materials to improve its efficacy. Among the six materials tested, peony (Paeonia suffruticosa) root bark (PRB) showed the strongest antifungal activity against R. solani AG2-1, and was not antagonistic to T. harzianum. Scanning electron microscopy showed that treatment with PRB extract resulted in shortened and deformed R. so/ani AG2-1 hyphal cells. The control of radish damping-off caused by R. so/ani AG2-1 was greatly increased by combined treatments of T. harzianum and PRB, as compared with either of the two treatments alone, with the control effect increased from 42.3-51.5% to 71.4-87.6%. The antifungal compound in PRB, which was isolated in chloroform and identified as paeonol by mass spectrometry, $^1H$ NMR, and $^{13}C$ NMR analyses, inhibited the growth of R. so/ani AG2-1 but not that of T. harzianum. Thus, PRB powder or extract may be used as a safe additive to T. harzianum to improve the control of the soil borne diseases caused by R. so/ani AG2-1.

• Research in Plant Disease
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• v.15 no.2
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• pp.101-105
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• 2009

The biocontrol agent Serratia plymuthica A21-4 was selected and proved as an excellent inhibitor of Phytophthora blight of pepper through in vitro and in vivo experiments in previous studies. To enhance the colonizing density of S. plymuthica A21-4 on plant root and rhizosphere soil, some soil conditions might effect on the colonization of the bacteria were examined. The results obtained from the study indicated that the soils containing more sand were favorable to root colonization of S. plymuthica A21-4. Organic amendment such as 3% maize straw(w/w) was helpful to colonize the bacteria in root and soil. The soil temperature about $20^{\circ}C$, water content around 40%, and soil pH near to neutral or slightly acidic, were optimum condition for the colonization of S. plymuthica A21-4 in the rhizosphere soil and roots of pepper. In addition, existence of indigenous biotic entities was beneficial to the colonization of S. plymuthica A21-4.

• Mycobiology
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• v.42 no.4
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• pp.346-352
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• 2014

Recently, the Q biotype of tobacco whitefly has been recognized as the most hazardous strain of Bemisia tabaci worldwide, because of its increased resistance to some insecticide groups. As an alternative control agent, we selected an Isaria javanica isolate as a candidate for the development of a mycopesticide against the Q biotype of sweet potato whitefly. To select optimal mass production media for solid-state fermentation, we compared the production yield and virulence of conidia between 2 substrates (barley and brown rice), and we also compared the effects of various additives on conidia production and virulence. Barley was a better substrate for conidia production, producing $3.43{\times}10^{10}$ conidia/g, compared with $3.05{\times}10^{10}$ conidia/g for brown rice. The addition of 2% $CaCO_3+2%$ $CaSO_4$ to barley significantly increased conidia production. Addition of yeast extract, casein, or gluten also improved conidia production on barley. Gluten addition (3% and 1.32%) to brown rice improved conidia production by 14 and 6 times, respectively, relative to brown rice without additives. Conidia cultivated on barley produced a mortality rate of 62% in the sweet potato whitefly after 4-day treatment, compared with 53% for conidia cultivated on brown rice. The amendment of solid substrate cultivation with additives changed the virulence of the conidia produced; the median lethal time ($LT_{50}$) was shorter for conidia produced on barley and brown rice with added yeast extract (1.32% and 3%, respectively), $KNO_3$ (0.6% and 1%), or gluten (1.32% and 3%) compared with conidia produced on substrates without additives.

• Journal of Microbiology and Biotechnology
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• v.22 no.7
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• pp.883-888
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• 2012

The efficacy of cowdung, Bangladesh Institute of Nuclear Agriculture (BINA)-biofertilizer, and Bangladesh Agricultural University (BAU)-biofungicide, alone or in combination, was evaluated for controlling foot rot disease of lentil. The results exhibited that BINA-biofertilizer and BAU-biofungicide (peat soil-based Rhizobium leguminosarum and black gram bran-based Trichoderma harzianum) are compatible and have combined effects in controlling the pathogenic fungi Fusarium oxysporum and Sclerotium rolfsii, which cause the root rot of lentil. Cowdung mixing with soil (at 5 t/ha) during final land preparation and seed coating with BINA-biofertilizer and BAU-biofungicide (at 2.5% of seed weight) before sowing recorded 81.50% field emergence of lentil, which showed up to 19.85% higher field emergence over the control. Post-emergence deaths of plants due to foot rot disease were significantly reduced after combined seed treatment with BINA-biofertilizer and BAU-biofungicide. Among the treatments used, only BAU-biofungicide as the seed treating agent resulted in higher plant stand (84.82%). Use of BINA-biofertilizer and BAU-biofungicide as seed treating biocontrol agents and application of cowdung in the soil as an organic source of nutrient resulted in higher shoot and root lengths, and dry shoot and root weights of lentil. BINA-biofertilizer significantly increased the number of nodules per plant and nodules weight of lentil. Seeds treating with BAU-biofungicide and BINA-biofertilizer and soil amendment with cowdung increased the biomass production of lentil up to 75.56% over the control.