• Title/Summary/Keyword: Ambient Media

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Sericin-Fixed Silk Fiber as an Immobilization Support of Enzyme

  • Lee Ki Hoon;Kang Gyung Don;Shin Bong Seob;Park Young Hwan
    • Fibers and Polymers
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    • v.6 no.1
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    • pp.1-5
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    • 2005
  • In this study, we attempted to evaluate a novel use of sericin-fixed silk fiber (SFx) as an immobilization support of enzyme. Sericin was fixed on the silk fiber using glutaraldehyde as a fixation reagent. After 6 hours of fixation, the degree of fixation increases linearly with linear decrease of the amount of bound $\alpha$-chymotrypsin (CT). This suggests that the increase of the degree of fixation is due to the further crosslinking of free aldehyde groups on the surface of sericin-fixed silk fiber (SFx). Even though perfect fixation was not achieved, sericin did not dissolve seriously and could be removed by further washing. The specific activity did not differ significantly after 6 hours of fixation. The activity of immobilized CT on SFx decreased to its half after 6 hours of incubation at 50$^{\circ}C$. However, it retained $78\%$ of initial activity even after 1 hour of treat­ment with $100\%$ ethanol. As a result, the SFx could be used as an immobilization support of enzyme in non-aqueous media at ambient temperature.

Surface Properties of the High Porous Carbon Aerogels (고다공성 카본 에어로젤(C-Aerogel) 표면 특성)

  • Kim, Ji-Hye;Lee, Chang-Rae;Jeong, Young-Soo;Kim, Yang-Do;Kim, In-Bae
    • Journal of the Korean institute of surface engineering
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    • v.41 no.3
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    • pp.114-120
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    • 2008
  • The pyrolysized carbon xerogel and aerogels were prepared from the sol-gel polymerization of resorcinol-formaldehyde(RF) followed by the dry process under ambient pressure and supercritical carbon dioxide condition respectively. The thermal behaviour of RF polymer xerogel was investigated with TGA analyzer to correspond with the pyrolysis process. The surface properties such as particle size, morphology and the point of zero charge of the pyrolysized porous carbon aerogels were studied for the precious metal catalyst supported media. It was found that the volume of the polymer aerogel decreased because of the significant linear shrinkage and weight loss of polymer gel during the carbonization. The point of zero charge of the carbon aerogel pyrolysized at $1050^{\circ}C$ under inert gas flow was about 10.

Comparative Reliability Analysis of DC-link Capacitor of 3-Level NPC Inverter Considering Mission-Profiles of PV Systems (태양광 시스템의 미션 프로파일 고려한 3-레벨 NPC 인버터의 DC-link 커패시터 신뢰성 비교 분석)

  • Jae-Heon, Choi;Ui-Min, Choi
    • The Transactions of the Korean Institute of Power Electronics
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    • v.27 no.6
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    • pp.535-540
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    • 2022
  • DC-link capacitors are reliability-critical components in a photovoltaic (PV) inverter. Typically, the lifetime of a DC-link capacitor is evaluated by considering the voltage and hot-spot temperature of the capacitor under the specific operating condition of the PV inverter. However, the output of the PV inverter is determined by solar irradiation and ambient temperature, which vary with the seasons; accordingly, the hot-spot temperature of the capacitor also changes. Therefore, the mission profile of the PV system should be considered to effectively evaluate the reliability of the DC-link capacitor. In this study, the reliability of the DC-link capacitor of a three-level NPC inverter is comparatively analyzed with and without considering the mission profiles of the PV system, where two mission profiles recorded in Arizona and Iza are considered. The accumulated damage of the DC-link capacitor is calculated based on the lifetime model by analyzing its thermal loading. Afterward, a reliability evaluation of the DC-link capacitor is performed at the component level and then at the system level by considering all capacitors by means of Monte Carlo analysis. Results reveal the importance of performing a mission-profile-based reliability evaluation during the design of high-reliability PV inverters to achieve the target reliability performance.

Depositional Characteristics of Atmospheric PBDEs on Pine Needles, Bark and Soil (대기 중 폴리브롬화디페닐에테르의 소나무 잎, 소나무 껍질 및 토양으로의 침착 특성)

  • Chun, Man Young
    • Journal of Environmental Health Sciences
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    • v.40 no.3
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    • pp.215-224
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    • 2014
  • Objective: This study was carried out in order to determine the depositional characteristics of pine needles, pine bark, and soil used as a passive air sampler (PAS) for atmospheric polybrominated diphenyl ethers (PBDEs). Methods: All three media were sampled from the same site. The PBDE concentrations were analyzed by HRGC/HRMS, and the lipid contents were measured using the gravimetric method by n-hexane extraction. Results: The total PBDE concentration was the highest in soil (22,274.57 pg/g dry), followed by pine bark (20,266.39 pg/g dry), and then pine needles (7,380.22 pg/g dry). Pine needles contained the highest lipid contents (21.31 mg/g dry), whereas soil (10.01 mg/g dry), and pine bark (4.85 mg/g dry) contained less. There were poor correlations between lipid content and total PBDE concentrations in the media ($R^2$=0.8216, p=0.2814). Congeners BDE 47, 99, 183, 196, 197, 206, 207 and 209 showed peak concentrations. Among these, BDE 206, 207, and 209 are highly brominated PBDEs that exist as particulates in ambient air. They accounted for 81.2% [69.2 (pine needles) - 89.0% (tree bark)] of the concentration and therefore are noted as the main congener of the total PBDEs. Conclusions: It can therefore be concluded that for reducing error by improper sampling, the same species of media should be recommended for use as a PAS for atmospheric PBDEs due to the differences in depositional characteristics.

Color Image Enhancement Using Vector Rotation Based on Color Constancy (칼라 항상성에 기초한 벡터 회전을 이용한 칼라 영상 향상)

  • 김경만;이채수;박영식;하영호
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 1996.06a
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    • pp.181-185
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    • 1996
  • Color image is largely corrupted by various ambient illumination. However, human perceives always white color as white under any illumination because of a characteristic of human vision, called color constancy. In the conventional algorithm which applied the constancy effect, after the RGB color space is transformed to the IHS(Intensity, Hue, and Saturation) color space, then the hue is preserved and the intensity or the saturation is properly enhanced. Then the enhanced IHS color is reversely transformed to the RGB color space. In this process, the color distortion is included due to the color gamut error. But in the proposed algorithm, there is not transformation. In that, the RGB color is considered as 3 dimensional color vector and we assume that white color is the natural daylight. As the color vector of the illumination can be calculated as the average vector of R, G, and B image, we can achieve the constancy effect by simply rotating the illumination vector to the white color vector. The simulation results show the efficiency of the vector rotating process for color image enhancement.

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Monaural Ambient Sound Extraction for On-line Audio Upmixing System based on Nonnegative Matrix Factorization (실시간 오디오 업믹싱 시스템을 위한 비음수 행렬 분해 기반의 단일채널 배경 잡음 추출 기법)

  • Lee, Seokjin
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2014.06a
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    • pp.5-8
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    • 2014
  • 본 논문에서는 비음수 행렬 분해 (NMF) 기법을 이용하여 단일 채널에서 배경음 성분을 추출하는 알고리즘에 대해 서술한다. 이러한 배경음 성분 추출은 오디오 업믹싱 시스템을 고려하여 개발되었으며, 기존의 연구를 통하여 분리된 배경음 신호가 서라운드 채널 혹은 상방향 채널에 적용될 경우 청취자의 공간감을 향상시킬 수 있다는 사실이 이미 확인된 바 있다. 다만 기존의 기법은 음향 신호를 모두 축적하여 일괄적으로 처리해야 한다는 단점이 있어, 스트리밍 시스템이나 디지털 신호 프로세서 등을 이용한 시스템에서 사용될 수 없는 단점이 있다. 본 논문에서는 이를 해소하기 위하여 실시간 비음수 행렬 분해 기법을 이용한 배경음 추출 시스템을 고안하여 실험하였다. 실험 결과 실시간 배경음 추출 기법이 신호의 후반부에서는 원하는 대로 동작하나, 초중반에 기저가 과도하게 설정되는 문제점이 있음을 확인할 수 있었으며, 이에 대한 해결이 향후 연구 과제가 될 것이다.

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"Turn-on" type colorimetric/fluorimetric probe for selective detection of Cu2+ at neutral pH condition

  • Lee, Hyun Jung;Saleem, Muhammad;Lee, Ki Hwan
    • Rapid Communication in Photoscience
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    • v.4 no.4
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    • pp.88-90
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    • 2015
  • The design and development of fluorescent chemosensors have recently been intensively explored for sensitive and specific detection of environmentally and biologically relevant metal ions in aqueous solution and living cells. Herein, we report the photophysical results of rhodamine B based fluorogenic and chromogenic receptor for selective copper detection in the complete organic or mixed aqueous-organic media at neutral pH under ambient condition. The ligand exhibited the remarkable increment in the fluorescence emission and UV-visible absorption signal intensities at 587 and 547 nm, respectively, on induction of copper ion while the ligand solution remain completely silent on addition of varieties of other metal ions.

Studies on thermal and swelling properties of Poly (NIPAM-co-2-HEA) based hydrogels

  • Shekhar, Suman;Mukherjee, M.;Sen, Akhil Kumar
    • Advances in materials Research
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    • v.1 no.4
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    • pp.269-284
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    • 2012
  • Thermoresponsive hydrogels based on N-Isopropylacrylamide (NIPAM) and 2-Hydroxyethylacrylate (HEA) were prepared by free radical polymerization. The hydrogels were characterized by elemental (CHN) analysis, differential scanning calorimetry (DSC) and thermo gravimetric analysis (TGA). DSC thermogram showed two endothermic transitions which are due to hydration of water present in different environments. One near $0^{\circ}C$ called melting transition of ice and was used to calculate the quantitative determination of the amounts of freezing and non freezing water. The other transition above the ambient temperature was due to the combination of hydrophobic hydration and hydrophilic hydration which changes with the copolymer compositions. Swelling and deswelling studies of the hydrogels were carried out using the aqueous media, salt and urea solutions. The experimental results from swelling studies revealed that copolymers have lower rates of swelling and deswelling than the homopolymer.

Position Detection of a Scattering 3D Object by Use of the Axially Distributed Image Sensing Technique

  • Cho, Myungjin;Shin, Donghak;Lee, Joon-Jae
    • Journal of the Optical Society of Korea
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    • v.18 no.4
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    • pp.414-418
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    • 2014
  • In this paper, we present a method to detect the position of a 3D object in scattering media by using the axially distributed sensing (ADS) method. Due to the scattering noise of the elemental images recorded by the ADS method, we apply a statistical image processing algorithm where the scattering elemental images are converted into scatter-reduced ones. With the scatter-reduced elemental images, we reconstruct the 3D images using the digital reconstruction algorithm based on ray back-projection. The reconstructed images are used for the position detection of a 3D object in the scattering medium. We perform the preliminary experiments and present experimental results.

Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique

  • Kwon Eun-Young;Kim Young-Tae;Kim Dae-Eun
    • KSTLE International Journal
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    • v.7 no.1
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    • pp.10-13
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    • 2006
  • In this work, imaging and study of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young's modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed ($20{\mu}m/ s$) compared to that in the ambient environment. For measuring the Young's modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young's modulus could be derived using the Hertz model. The Young's modulus of living L-929 fibroblast cell was $1.29{\pm}0.2$ kPa.