• The Korean Journal of Physiology and Pharmacology
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• 제23권3호
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• pp.171-179
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• 2019

Pituitary tumors are usually benign but can occasionally exhibit hormonal and proliferative behaviors. Dysregulation of the G1/S restriction point largely contributes to the over-proliferation of pituitary tumor cells. F-box protein S-phase kinase-interacting protein-2 (SKP2) reportedly targets and inhibits the expression of $p27^{Kip1}$, a well-known negative regulator of G1 cell cycle progression. In this study, SKP2 expression was found to be upregulated while $p27^{Kip1}$ expression was determined to be downregulated in rat and human pituitary tumor cells. Furthermore, SKP2 knockdown induced upregulation of $p27^{Kip1}$ and cell growth inhibition in rat and human pituitary tumor cells, while SKP2overexpression elicited opposite effects on $p27^{Kip1}$ expression and cell growth. The expression of microRNA-186 (miR-186) was reported to be reduced in pituitary tumors. Online tools predicted SKP2 to be a direct downstream target of miR-186, which was further confirmed by luciferase reporter gene assays. Moreover, miR-186 could modulate the cell proliferation and $p27^{Kip1}$-mediated cell cycle alternation of rat and human pituitary tumor cells through SKP2. As further confirmation of these findings, miR-186 and $p27^{Kip1}$ expression were downregulated, while SKP2 expression was upregulated in human pituitary tumor tissue samples; thus, SKP2 expression negatively correlated with miR-186 and $p27^{Kip1}$ expression. In contrast, miR-186 expression positively associated with $p27^{Kip1}$ expression. Taken together, we discovered a novel mechanism by which miR-186/SKP2 axis modulates pituitary tumor cell proliferation through $p27^{Kip1}$-mediated cell cycle alternation.

• Journal of the korean society of oceanography
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• 제33권3호
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• pp.71-79
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• 1998

Silty tidal rhythmites were found from the upper Pleistocene sequence unconformably overlain by the Holocene tidal deposits within the macrotidal coastal zone of Youngjong Island, western coast of Korea. The rhythmites occur as vertically accreted, parallel and planar laminae that are 0.1-2.5 mm in thickness. Each lamina grades from coarse silt (mean grain size: 5-6.5 ${\phi}$) at the lower part into fine silt to mud (mean grain size: 6-7.5 ${\phi}$) at the upper part. The rhythmites can be classified into two types based on the patterns in laminar thickness variation. Type I is a bundle of 12-20 laminae in which laminar thickness varies sinusoidally. Type ll is an alternation of thick and thin laminae as a couplet. Type I is inferred as a product of varying tidal energy during a semimonthly (neap-spring) tidal cycle, in which thicker laminae were deposited during spring tides and thinner laminae were formed during neap tides. Type ll is interpreted to have been formed by asymmetric semidiurnal tidal currents in association with diurnal inequality, whereby thick lamina of each couplet represents dominant tidal current and the thin lamina reflects subordinate tidal current.

• 한국실내디자인학회논문집
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• 제40호
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• pp.42-51
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• 2003

This study aimed at understanding the spatial characteristics in the Tugendhat house. For this study, it is need to analyze the early plans of the Tugendhat house design process. To analyze this, the study leads to how to effect on the real practice with the process. Therefore, this research investigates the conceptional design process of the house. There are 2 main characteristics of the interior space alternation. One is the closed spatial character on the 2nd floor, the other is the open spatial character on the 1st floor. It clearly shows the severance between interior and exterior on the 2nd floor. Each 2nd floor's space volume uses different materials so that each the space can show the individuality. On the 1st floor, however, it seems the interior is not as much open to the exterior, because the materiality and reflection of the glass, the objet of furniture and wall. In case of 1st floor, it has a complex concept as open and close character, because it is using the proper gardening elements and the relationship between the house and site. The exterior characteristic is separated the house from the ground with the podium and the slope, and this leads to have the discontinuity. The house clearly shows the spatial characteristics of the continuity and discontinuity, it proves that Mies tried to move onto modem architecture as a turning point.

• 복식문화연구
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• 제17권5호
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• pp.807-818
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• 2009

This study is to present a female high school summer blouse with high movement functionality and satisfying appearance, surveyed the students for their understanding of summer blouse. Experimental study's research method was questionnaire survey. By using questionnaires, the satisfaction and comfort of each region of the current worn summer blouse was analyzed. The results of this research are as follows. After surveying 371 high school students in the Daejeon region on the currently worn summer blouse comfort levels, 48.4% showed dissatisfaction about the width of the sleeves and the armpits and over 93% wanted the use of elastic materials as an alternative. After investigating the summer blouses' mending state, 41% of students that mending their clothes chose the alternation of reducing the overall silhouette of the blouse in all areas. This reflects the female high school students' mentality that thin body shape is the ideal.

• International Journal of Naval Architecture and Ocean Engineering
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• 제6권2호
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• pp.406-417
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• 2014

This paper presents a numerical optimization method to improve the performance of the propeller with Turbo-Ring using real-coded genetic algorithm. In the presented method, Unimodal Normal Distribution Crossover (UNDX) and Minimal Generation Gap (MGG) model are used as crossover operator and generation-alternation model, respectively. Propeller characteristics are evaluated by a simple surface panel method "SQCM" in the optimization process. Blade sections of the original Turbo-Ring and propeller are replaced by the NACA66 a = 0.8 section. However, original chord, skew, rake and maximum blade thickness distributions in the radial direction are unchanged. Pitch and maximum camber distributions in the radial direction are selected as the design variables. Optimization is conducted to maximize the efficiency of the propeller with Turbo-Ring. The experimental result shows that the efficiency of the optimized propeller with Turbo-Ring is higher than that of the original propeller with Turbo-Ring.

• 자원환경지질
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• 제9권3호
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• pp.157-163
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• 1976

In this study, the oil bearing rock-sequence, U-hand-ri Formation (D.S. Lee et al., 1976), was subdivided into three members; the lower, the intermediate and the upper. The lower consists mainly of reddish purple tuff and sandy calcareous shales, the intermediate of an alternation of tuffs, sandstons, calcareous black shales, cherts and limestone and the upper of coarse grained variegated tuff and agglomerate. Oily matter was found from the black shales of the intermediate. Ten samples of black shales from drilled cores, 8 samples of black shales from different outcrops of the member, and 1 sample of grease-like seeping oil from black shales at U-hang-ri coast were chemically analyized. Among them, 9 samples contain remarkable amount of organic carbon (0.96~1.60%) and E.O.M. extract (0.176~0.718%), and mostly the bituminous material is saturated hydrocarbons as well as shown in infared spectroscopic analyses. The elemental analyses of MAE extracts and asphaltenes of some of thoese samples indicate that the transformation of organic material to crude oil is highly progressed. The authors suggest that the seeping oil and oily tinges in black shale layers are the products of natural cracking related with the igneous activities in the area nearby.

• 한방재활의학과학회지
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• 제19권1호
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• pp.187-199
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• 2009

Objectives : The electroacupuncture was generally has been used in oriental medicine doctors. In recent years, there have been a few studies about safety and stability of acupuncture needle in itself, but then again research of acupuncture needle safety and stability on applying electroacupuncture have been insignificant. Therefore we investigated the safety and change in mechanical characteristic of acupuncture needle in electroacupuncture therapy. Methods : We observed mechanical characteristic change by SEM(Scanning Electron Microscope) and EDX(Energy Dispersive X-ray Spectroscopy), evaluated the hardness by vickers hardness tester. We used MTT assay and cell stain to study about biocompatibility of electroacupuncture. Results : In this study, any corrosion of material, alternation of elements, and change of hardness were not observed in surface analysis using SEM and EDX. In cytotoxity evaluation using MTT assay and cell stain, cell survival rate was low when practicing the electroacupuncture for more than 3 hours. Conclusions : Change of mechanical property was not observed based on the test results using surface analysis and hardness estimation by the electroacupuncture. And considering the biocompatibility, electroacupuncture was thought to be safe in an hour based on cytotoxity evaluation using MTT assay and cell stain.

• 한국응급구조학회지
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• 제17권1호
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• pp.9-19
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• 2013

Purpose : The purpose of this study was to investigate the variation of elapsed time in the cardiopulmonary resuscitation (CPR) quality and the fatigue in continuous CPR by single rescuer. This study will provide basic data about the time for the alternation of the CPR providers. Methods : The volunteer students having healthcare provider certification were recruited from the department of emergency medical service. The students performed 30:2 CPR for 20 minutes, and the data were recorded and analyzed. Metrics were based on the 2010 American Heart Association (AHA) Guidelines, and the CPR continued without any feedback. Results : Among the indicators of CPR, the accuracy and the depth of chest compressions decreased after about 5.3 minutes, and the rate increased approximately after 6.8 minutes. Changes in clinical indicators appeared, and fatigue increased after about 3 minutes. According to the increase in fatigue level, the changes in the CPR indicators and clinical indicators showed up, and these results proved to be statistically significant. However, there were no associations among the time, fatigue, and gender. Conclusion : Even though the times of changes in the indicators appeared differently, the times of changes in fatigue and CPR quality were able to be confirmed.

• Asian-Australasian Journal of Animal Sciences
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• 제27권1호
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• pp.147-154
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• 2014

This review focuses on modulation of growth hormone (GH) and its downstream actions on periparturient dairy cows undergoing physiological and metabolic adaptations. During the periparturient period, cows experience a negative energy balance implicating that the feed intake does not meet the total energy demand for the onset of lactation. To regulate this metabolic condition, key hormones of somatotropic axis such as GH, IGF-I and insulin must coordinate adaptations required for the preservation of metabolic homeostasis. The hepatic GHR1A transcript and GHR protein are reduced at parturition, but recovers on postpartum. However, plasma IGF-I concentration remains low even though hepatic abundance of the GHR and IGF-I mRNA return to pre-calving value. This might be caused by alternation in IGFBPs and ALS genes, which consequently affect the plasma IGF-I stability. Plasma insulin level declines in a parallel manner with the decrease in plasma IGF-I after parturition. Increased GH stimulates the lipolytic effects and hepatic glucose synthesis to meet the energy requirement for mammary lactose synthesis, suggesting that GH antagonizes insulin-dependent glucose uptake and attenuates insulin action to decrease gluconeogenesis.

• International journal of advanced smart convergence
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• 제6권4호
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• pp.41-49
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• 2017

In this paper I applies Borer (1993)'s way of classifying English intransitive action verbs such as 'run', walk, among many others, to the corresponding Korean intransitive action verbs such as 'tali-ta' and 'keət-ta', and show how they are different from - or similar with - each other in terms of syntactic structures and verb classification. Unlike the English verb 'run' which can be classified into an unaccusative verb as well as an unergative verb in Borer's theory, the corresponding Korean verbs 'tali-ta' or 't'wi-ta' can behave not only as an unergative and unauucsative verb, but also it can behave as a transitive verb. Though Borer's perspective on classification of verb types may be thought of as somewhat radical mostly due to its heavy dependency on aspectual representation of a whole sentence which a verb is just part of, it is clearly suggesting a new and great insight into the controversial topic of classification of verb types. So it is worth adopting this insightful perspective for the analysis of corresponding Korean verbs and seeing if it also works for the Korean ones.