• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.5
• /
• pp.753-762
• /
• 2020

Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.

• Journal of architectural history
• /
• v.23 no.6
• /
• pp.7-20
• /
• 2014

The purpose of this paper is to study on the architecture and extension project for the Church of St. Lorenzo. This study is composed of a through analysis of the extension elements on the church building and extension type for extension project of the Church of St. Lorenzo. The results of study are as follows: 1. The extension project for the Church of St. Lorenzo was basically consisted of the concept of a horizontal extension plan through both the interior and exterior space of the church building. This project was the plan of concept of additional or affixing extension through existing spaces with necessary spaces to make up for the previous simple space and form as well as functional aspect. 2. The unit spaces of the nave, the aisle, the small chapels bilaterally adorning the aisles, the transept and the chapels around transepts, and the sacristies by the extension project for the Church of St. Lorenzo is composed of regulation, balance and harmony as geometric space composition according to simple proportion system on whole and parts as well as parts and parts, through the definite articulation by each space zoning. 3. The most important innovative aspect different to previous churches in the extension project for the Church of St. Lorenzo is the compositive system of each other symmetrical spaces through long centrical axis of central part, applying the module system for horizontal arrangement. In particular, the use of regular module on repetition and proportion of rhythm from architectural composition system was precisely composed with the alter that consists of visual focus to express more accurate perspective.

• Polymer(Korea)
• /
• v.38 no.3
• /
• pp.314-319
• /
• 2014

Three kinds of thermal stabilizers for nylon 4 were synthesized to incorporate both hindered amine groups and methylene units with various lengths. It is expected that the hindered amine groups play a role in the capture of degradation-triggering species. Considering sequence rules, hydrogen bonding formed between nylon 4 and the stabilizers is optimized to alter the lengths of the methylene units in the stabilizers. As a result, it was found that a tetramethylene unit in the stabilizer is an optimal length for hydrogen bonding in terms of isothermal thermogravimetric analysis (TGA). Considering the slight and often negligible improvement of thermal stability of nylon 4 containing commercially-available nylon 6 stabilizers, retardation of thermal degradation has been substantially improved upon.

• The KIPS Transactions:PartC
• /
• v.10C no.6
• /
• pp.675-682
• /
• 2003

Attackers collect vulnerabilities of a target computer system to intrude into it. And using several attack methods, they acquire root privilege. They steal and alter information in the computer system, or destroy the computer sysem. So far many intrusion detection systems and firewallshave been developed, but recently attackers go round these systems and intrude into a computer system . In this paper, we propose security kernel module to prevent attackers having acquired root privilege from doing illegal behaviors. It enhances administrator authentication with additional password, so prevents attackers from doing illegal behaviors such as modification of important files and installation of rootkits. It sends warning mail about sttacker's illegal behaviors to administrators by real time. So using information in the mail, they can estabilish new security policies.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.17 no.1
• /
• pp.64-80
• /
• 1991

The SKINTEX Method is based on a two-compartment physico-chemical model which includes a Biomembrane Barrier in compartment one and an organized macromolecular matrix in compartment two. Test samples absorb onto or permeate through the keratin/collagen Biomembrane Barrier and then can interact with the organized macromolecular matrix. Changes in the integrity of the barrier release a dye indicator: Changes in the matrix can alter its transparency. The sum of these two responses is read spectrophotometrically at 470nm. An early investigation of 950 chemicals and formulations in the SKINTEX System produced results which were 89% concordance to in vivo Draize dermal irritation results obtained with 24-hour occluded application of test samples with-out abrasion and standard scoring. Alkaline materials were analyzed in a specialized SKINTEX AMA Protocol. In this early study, the model did not distinguish nonirritant test materials and formulation with PDII(Primary Dermal Irritation Index)in the range from 0 to 1.2, A High Sensitivity Assay Protocol(HSA)was developed to amplify the changes in both compartments of this model and provide more accurate calibration of these changes. A study of 60 low irritation test samples including cosmetics, household products, chemicals and petro-chemicals distinguished nonirritants with PDII $\leq$ 0.7 for 26 of 30 nonirritants. A second protocol was developed to evaluate the SKINTEX model predictability with respect to human irritation. The Human Response Assay (HRA )has been optimized based on differences in penetration and irritation responses in humans and rabbits. An additional 32 test materials with different mechanisms and degrees of dermal toxicity were evaluated by the HRA. These in vitro results were 86% concordant to human patch test results. In order to further evaluate this model, a Standard Chemical Labelling (SCL) Protocol was developed to optimize this system to predict Draize dermal irritation results after a 4-hour application of the test material. In a study of 52 chemicals including acids, bases, solvents, salts, surfactants and preservatives, the SCL results demonstrated 85% concordance to Draize results for a 4-hour application of test samples on non-abraded rabbit skin. The SKINTEX System, including three specialized protocols, provided results which demonstrated good correlation to the endpoint of dermal irritation in man and rabbits at different application times.

• Toxicological Research
• /
• v.20 no.3
• /
• pp.251-261
• /
• 2004

A large number of chemical pollutants including phthalates, alkylphenolic compounds and organochlorine pesticides have the ability to disrupt endocrine function in animals, and alter cog-nitive function. Because hormone mediated events play an important role in central nervous system development and function, the changes in cognitive function seem to be mediated by the endocrine-like action of these chemicals. The present study therefore was designed to investigate effect of bisphenol A (BPA), an endocrine disrupting chemical on neuro-behavial patterns, and expression of estrogen receptors and tyrosine hydroxylase, a limiting enzyme of dopamine synthesis pathway. BPA was treated orally for 3 weeks into 3 week old mice, and then the neuro-behavial patterns (stereo-type behaviors such as jumping rearing and forepaw tremor, climbing behavior, tail flick, rotarod and locomotor activity), and the expression of estrogen receptors and tyrosine hydroxylase were deter-mined every 3 week for 9 weeks. During the treatment of BPA, the food uptake and body weight increase were not significantly changed. BPA resulted in the increased stereotype behaviors (jump-ing, rearing and forepaw tremor) 6 or 9 weeks after treatment. The time response to tail flick and locomotor activity were decreased by the treatment of BPA, whereas the time for rotarod was increased by the treatment of BPA. The expression of estrogen receptor alpha and beta was increased in the brain and pituitary gland. Maximum expression was found in the brain after 9 week of 100 mg/kg BPA treatment and in the pituitary gland after 6 week of 100 mg/kg BPA treatment. Tyrosine hydroxylase was increased in dose and time dependent manners in the brain but no change was found in the pituitary gland. The present data show that exposure of BPA in the young mice could alter expression of estrogen receptors and dopamine synthesis pathway, thereby modulate neuro-behavial patterns (increase of stereotype behaviors but decrease locomotor activity).

• Asian-Australasian Journal of Animal Sciences
• /
• v.10 no.2
• /
• pp.233-239
• /
• 1997

A mammary epithelial cell line (MAC-T) established as a model for lactation was utilized to identify and characterize effects of various hormones upon insulin-like growth factor binding protein secretion. Ligand and immunoblot analyses of conditioned media indicated that insulin-like growth factor binding protein-2 was secreted by MAC-T cells. Insulin-like growth factor-I stimulated insulin-like growth factor binding protein-2 secretion in a dose-dependent manner, but prolactin and bovine somatotropin did not alter insulin-like growth factor binding protein-2 secretion. Insulin increased and cortisol decreased insulin-like growth factor binding protein-2 secretion. Effects of insulin-like growth factor-I on insulin-like growth factor binding protein-2 secretion support previous studies using primary cultures of bovine mammary cells and bovine fibroblasts. Effects of cortisol and insulin on insulin-like growth factor binding protein-2 secretion may be explained by changes in protein synthesis. In addition, supraphysiological doses of insulin can cross-react with the insulin-like growth factor-I receptor and stimulate insulin-like growth factor binding protein-2 secretion. MAC-T cells provide a model system to study mechanisms that regulate local insulin-like growth factor-I bioactivity.

• Management & Information Systems Review
• /
• v.17
• /
• pp.289-313
• /
• 2005

Electronic commerce is giving rise to many new and innovative 'gest business practices,' such as telecommuting and the virtual workplace. Telecommuting and the virtual workplace go hand in hand. Today, more then 35 million people in the United States telecommute, and that expected to grow by 20 percent over the next several years. And more then 30 million people used internet shopping mall every years in Korea. Therefore, using the internet and information technology, electronic commerce has the potential to propel a company to 'break out' of existing strategic constraints and radically alter business processes, strengthen customer and supplier ties, and open up new markets. The object of this study is the investigation method for activity solution of electronic commerce of fashion cloth shopping mall in korea. The study has investigated 2 cases about 'halfclub.com' in Korea and 'bluefly.com' in America in the research. Therefore, the study founded the 3problems of electronic commerce at fashion cloth shopping mall in korea and suggested that the activation policies of electronic commerce. The result of suggested that the new electronic commerce style, a lot of contents affects positive effects on the performance of fashion cloth shopping mall in Korea and hope that will be expect new role of electronic commerce to be the herb in fashion cloth shopping mall in korea.

• Journal of the Korea Society of Computer and Information
• /
• v.4 no.1
• /
• pp.85-91
• /
• 1999

The first line school system is complexed. In result school affairs is subdividing and complicating more and more. It increased school affairs of teachers and they spend more soluting of their work than studying of teaching materials . The performance evaluation was introduced into the management of school record work as a part of educational reform. This is the evaluation of a studying course and a heavy burden of teachers was aggravated. So they have to depend on computer. In this study. I want to present the efficient method of management of school record work by using Hnagul 97 and Hangul approach 97. That will be able to make out a record easily. It is possible to design a individual pattern and to alter it.

• Biomolecules & Therapeutics
• /
• v.7 no.4
• /
• pp.390-396
• /
• 1999

CJ-50005 is a hepatitis A vaccine which is prepared by formalin inactivation of the HM175 virus cultured in human diploid MRC-5 cells. The general pharmacological properties of CJ-50005 were evaluated in various animals and in vitor system. CJ-50005 at the doses of 0.025, 0.25 and 0.75 $\mu\textrm{g}$/kg, i.m. had no effect on general behavior in mice, chemo- and electro-convulsions in mice, writhing syndrome induced by acetic acid in mice, the barbital sleeping time in mice, body temperature in rats, charcoal meal propulsion in mice and urine and electrolytes excretion in rats. In anesthetized dogs, CJ-50005(0.25 and 0.75 $\mu\textrm{g}$/kg, i.v) did not alter the respiratory rate, blood pressure, heart rate, femoral blood flow and ECG. In in vitro experiment, CJ-50005 at the concentration up to 0.02 $\mu\textrm{g}$/ml did not produce any changes in the contractions of the isolated ileum of guinea pigs caused by acetylcholine, histamine or $BaCl_2$. Since these pharmacological effects of CJ-50005 were observed at dose much greater than those in clinical use (approximately 0.025 $\mu\textrm{g}$/kg, i.m.), it is likely that this vaccine may be relatively free of undesirable effects in clinical practice.