• 식물분류학회지
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• 제53권3호
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• pp.230-236
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• 2023

Rhododendron caucasicum Pall. is a shrub distributed in the mountainous areas of the Caucasus from northeastern Türkiye towards the Caspian Sea. This study reports the first complete chloroplast genome sequence of R. caucasicum. The plastome is 199,487 base pairs (bp) long and exhibits a typical quadripartite structure comprising a large single-copy region of 107,645 bp, a small single-copy region of 2,598 bp, and a pair of identical inverted repeat regions of 44,622 bp each. It contains 143 genes, comprising 93 protein-coding genes, 42 tRNA genes, and eight rRNA genes. The large chloroplast genome size is likely due to the expansion of inverted repeats. A phylogenetic analysis of chloroplast genomes with other Rhododendron species supports previously recognized infrageneric relationship.

• 식물조직배양학회지
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• 제25권2호
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• pp.89-93
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• 1998

Tomato phenylalanine ammonia-lyase 5 (tPAL5) was identified that alternate initiation sites were utilized differentially in response to environmental stimuli (Lee et al, 1992b). In this study, we tried to look into tissue -or cell- specific expression pattern of tPAL5 gene by fusing with ${\beta}-glucuronidase$ (GUS) gene in transgenic tobacco plants. In transgenic plants, root and stem extracts contained 8~12 fold higher levels of GUS activity than petiole or leaf tissue while the highest levels of induction was observed from leaf tissue by mechanical wounding (5~11 fold). In trans-sections of stems and petioles, GUS activity was restricted to phloem cells(outer region) of developing vascular bundle and mainly at apical tip region in the root tissues. The levels of GUS activity was drastically reduced (10~12 fold reduction) when the 5'-upstream region of tPAL5 gene (-1151bp from ATG codon) was deleted up to -665. The levels of GUS expression, however, raised up by 6~8 fold when deleted up to -455. Therefore, we conclude that there are positive cis-elements at the region -1151 to -1008 and at -455 to -195 while the negative cis-element is at -1008 to -455.

• Journal of Ecology and Environment
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• 제42권1호
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• pp.20-29
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• 2018

Background: For various reasons such as agricultural and economical purposes, land-use changes are rapidly increasing not only in Korea but also in the world, leading to shifts in the characteristics of local carbon cycle. Therefore, in order to understand the large-scale ecosystem carbon cycle, it is necessary first to understand vegetation on this local scale. As a result, it is essential to comprehend change of the carbon balance attributed by the land-use changes. In this study, we attempt to understand accumulated soil carbon (ASC) and soil respiration (Rs) related to carbon cycle in two ecosystems, artificially turned forest into pastureland from forest and a native deciduous temperate forest, resulted from different land-use in the same area. Results: Rs were shown typical seasonal changes in the alpine pastureland (AP) and temperate deciduous forest (TDF). The annual average Rs was $160.5mg\;CO_2\;m^{-2}h^{-1}$ in the AP, but it was $405.1mg\;CO_2\;m^{-2}h^{-1}$ in the TDF, indicating that the Rs in the AP was lower about 54% than that in the TDF. Also, ASC in the AP was $124.49Mg\;C\;ha^{-1}$ from litter layer to 30-cm soil depth. The ASC was about $88.9Mg\;C\;ha^{-1}$, and it was 71.5% of that of the AP. The temperature factors in the AP was high about $4^{\circ}C$ on average compared to the TDF. In AP, it was observed high amount of sunlight entering near the soil surface which is related to high soil temperature is due to low canopy structure. This tendency is due to the smaller emission of organic carbon that is accumulated in the soil, which means a higher ASC in the AP compared to the TDF. Conclusions: The artificial transformation of natural ecosystems into different ecosystems is proceeding widely in the world as well as Korea. The change in land-use type is caused to make the different characteristics of carbon cycle and storage in same region. For evaluating and predicting the carbon cycle in the vegetation modified by the human activity, it is necessary to understand the carbon cycle and storage characteristics of natural ecosystems and converted ecosystems. In this study, we studied the characteristics of ecosystem carbon cycle using different forms in the same region. The land-use changes from a TDF to AP leads to changes in dominant vegetation. Removal of canopy increased light and temperature conditions and slightly decreased SMC during the growing season. Also, land-use change led to an increase of ASC and decrease of Rs in AP. In terms of ecosystem carbon sequestration, AP showed a greater amount of carbon stored in the soil due to sustained supply of above-ground liters and lower degradation rate (soil respiration) than TDF in the high mountains. This shows that TDF and AP do not have much difference in terms of storage and circulation of carbon because the amount of carbon in the forest biomass is stored in the soil in the AP.

• 농업과학연구
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• 제51권1호
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• pp.25-39
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• 2024

This study aimed to determine a method for restoring grasslands to forests in mountainous areas. The study was conducted in an area damaged by trampling due to construction activity and herding. Additionally, there were concerns of hindered tree growth due to the alpine region climate, characterized by the low temperatures, drying, and strong winds. Therefore, forest restoration treatments such as tillage, furrow, and shading were performed to improve growth conditions. From July 2021 to April 2023, soil temperature and moisture were monitored for each treatment plot, and changes in the initial growth and survival rate of Abies holophylla were investigated. Both soil temperature and soil moisture showed significantly higher values during the four seasons in the grassland-furrow treatment plot (p < 0.05). Compared to the grassland-control plot, the average soil temperature in winter was as high as 1.1℃, and the soil moisture in summer was as high as 20.3%. Additionally, the survival rate and relative growth rate were the largest in the grassland-furrow treatment plot. The survival rate was 87.5%, the relative growth rate in terms of height was 0.013, and the relative growth rate in terms of diameter at root collar was 0.023, indicating that furrow treatment had a positive effect on the initial survival and growth of seedlings. These results suggest that furrow treatment may be an appropriate restoration method when implementing forest restoration projects in areas where tree growth is challenged.

• 한국식물병리학회지
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• 제14권4호
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• pp.364-366
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• 1998

Bacterial soft rot was occurrenced on stems of cauliflower at a trial field of Alpine Agricultural Experiment Station, Kangwon-Do, Korea. the symptoms began as a small water-soaked lesion, which enlarged rapidly in diameter. The tissue within the affected region became slimy, disintegrating into a mushy mass of disorganized cells. The causal organism was isolated from the diseases lesions and was identified as Erwinia carotovora subsp. carotovora based on the morphological, physiological and chemical characteristics , and on the results of the Biolog program (Biolog Inc., U. S. A.). E. carotovora subsp. carotovora is the first described bacterium which causes bacterial soft rot on cauliflower in Korea.

• 식물분류학회지
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• 제53권1호
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• pp.65-68
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• 2023

The herb Pedicularis cheilanthifolia var. albida (Pennell) P. C. Tsoong is reported and collected for the first time from the Rudraprayag District of Uttarakhand. The species was seen growing along a glacial stream in an alpine region between 4,100 and 4,400 m asl. Morphological characteristics of the species were examined and processed for herbarium deposition. To simplify taxon identification, a brief taxonomic description and illustration of the taxon are provided.

• The Plant Pathology Journal
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• 제18권3호
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• pp.130-137
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• 2002

Potato virus Y (PVY) is one of the most important viruses in many field crops in Korea. In this study, 31 PVY isolates were isolated from infected potato (Solanum tuberosum), tobacco (Nicotiana tabacum), pea (Pisum sativum), and weeds (Veronica persica, Lamium amplexicause and Capsella bursa-pastoris) showing different mosaic symptoms in Jeonbuk, Chungnam, Gangwon, and Gyeongbuk areas in Korea. The 640 nucleotide region containing the C-terminal portion of coat protein (CP) gene and 3'non-translated region (NTR) was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using PVY-specific oligonucleotide primers. Sequence analyses of the amplified DNA fragments showed that the C-terminal portion of CP gene was not significantly different from that of previously reported PVY strains from potato (PVY-OK and -T) and tobacco (PVY-VN) in Korea. Homologies of the deduced CP amino acid sequences were 93.3-99.0% to corresponding regions of the other PVY strains including PV $Y^{N}$, PV $Y^{o}$ , PV $Y^{OK}$ , PV $Y^{T}$ , and PV $Y^{VN}$ . In contrast the sequences located at the 3'-NTR showed more diverse sequence homologies (76.4-99.7%). These results indicate that the C-terminal portion of the CP gene was relatively conserved while sequences at the 3'NTR were more diverse and variable over the host species and the regions where they were isolated.e isolated.

• Journal of Plant Biotechnology
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• 제37권1호
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• pp.89-95
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• 2010

Dihydroflavonol 4-reductase(DFR)는 flavonoid 생합성 경로의 가장 중심부에 작용하는 효소로 2R,3R-trans-dihydroflavonols로부터 leucoanthocyanidins 으로의 변환을 촉매한다. 본 연구에서는 색소유전자의 전이를 통하여 새로운 색소발현체계를 가진 품종을 육종하기 위한 기초연구로 안개초 (Gypsophila paniculata L.)의 꽃봉오리로부터 cDNAlibrary를 합성하였고 카네이션의 DFR 유전자를 probe로 사용하여 anthocyanin 합성경로의 중요 효소의 하나인 DFR 유전자를 분리하였다. 염기서열분석을 수행하여 분리유전자의 크기가 1279 bp이며 이 중 coding region은 1063 bp임을 확인하였다. 이미 밝혀진 다른 식물체의 DFR 유전자와 서로 염기서열의 일치성을 비교해 본 결과 Cheddar pink, 카네이션, 양배추, 개나리, 페튜니아, cup flower, 장미, 과꽃 및 거베라에서 각각 62% 이상을 나타내었다. 분리유전자의 발현을 확인하기 위하여 Northern blot 분석 및 인위적으로 기내에서의 transcription과 translation을 수행하였고, 분리한 유전자의 효소활성을 측정해 본 결과 leucopelargonidin의 작은 peak를 확인하였다. Southern blot 분석 결과 안개초의 DFR 유전자는 다른 대부분의 식물체와 유사하게 한 개가 존재함을 확인하였다.

• 한국환경생태학회지
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• 제18권2호
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• pp.107-123
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• 2004

백두대간 덕유산지역 일대에 분포하는 식물상을 조사한 결과 91과 243속 353종 53변종 5품종 총 411종류가 확인되었다. 개비자나무, 생강나무, 대팻집나무 및 노각나무 등이 나타나 남부아구로 볼 수 있으나 거제수나무, 사스래나무, 처녀치마 및 금강애기나리 등 중부아구 식물도 확인되었으며, 해발 1,000m 이상인 곳에는 고산식물이 많이 분포하였다. 산림청이 지정한 희귀 및 멸종위기종은 솔나리와 산작약, 그리고 구상나무, 금강애기나리, 말나리, 뻐꾹나리, 누른종덩굴, 등대시호, 흰참꽃 및 꽃창포 등으로 총 10종이 확인되었다. 환경부지정 보호 야생 동ㆍ식물에 포함되어 있는 종은 솔나리 1종이 확인되었다. 한국특산식물종은 개비자나무, 구상나무, 일월비비추, 죽대, 뻐꾹나리 등 12과 21속 20종 1품종 1변종으로 총 22종류가 확인되었다.

• 한국작물학회지
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• 제55권3호
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• pp.214-219
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• 2010

지구온난화에 따른 북한지역의 식량작물 생산을 주축으로 하는 이모작의 특성을 평가하기 위해서 온난화의 지역적 특성, 겨울작물의 월동온도, 여름작물의 냉해유발온도 및 이모작 작부양식별 소요적산온도 확보 등 온열지표의 변화를 농업기후지대별로 검토한 결과는 다음과 같다. 1. 온난화의 지역적 특성 북한의 온난화는 과거(1973-1994)보다 최근(2002-2006) 들어 연간 평균기온 $3,271^{\circ}C$로 $233^{\circ}C$나 상승되었고 일평균기온은 $8.96^{\circ}C$로 $0.64^{\circ}C$ 증가되었다. 일평균기온의 지역간 변이는 아한대인 북부내륙고산지대의 삼지연에서 $1.06^{\circ}C$, 온대인 동해안중부지대의 장전에서 $12.26^{\circ}C$로 큰 변이를 보였다. 2. 겨울작물의 월동가능지역 확대 겨울작물의 월동온도를 기준으로 가을보리+벼 이모작 ($-13^{\circ}C$ 이상 지역) 가능지역의 분포는 과거 서해안과 동해안 및 서부중간지대의 일부에서 최근 들어 서부중간지대의 전역으로 확대되었고, 가을밀+벼 이모작($-15^{\circ}C$) 가능지역은 과거 서해안과 동해안 및 서부중간지 전역에서 최근 중부산간지대의 전역과 북부산간 일부지역까지 확대되었다. 3. 여름작물의 냉해우려지역 분포 7월중 일평균기온 $17^{\circ}C$이하의 장해형냉해 유발온도의 경과일수를 기준으로 냉해우려지역의 분포는 북부내륙고산지대에서 21-29일, 동북해안북부지대에서 8-10일, 동북해안남부지대에서 2-5일로 나타났다. 4. 이모작 적산온도 확보의 지역적 분포 월동작물 가을밀과 결합된 여름작물(벼, 옥수수 및 콩) 이모작을 가능케 하는 적산온도 $3,150^{\circ}C$ 이상 확보지역의 분포는 북부내륙고산지대를 제외한 북한 전역으로 확대되었고, 여름작물 봄감자와 결합된 벼, 옥수수 및 콩 등 이모작을 가능케 하는 적산온도 $2,650^{\circ}C$ 이상 확보지역의 분포는 일부 북부내륙고산지대 혜산지역까지 가능하였다.