• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.406.3-407
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• 2002

Analysis of interferon alpha (IFN) modified with high molecular weight branched PEG was performed by capillary electrophoresis (CE) and MALDI-TOF mass spectrometry (MALDI-TOF MS). IFN was modified by the reaction of amine residues with an active ester of monomethoxy polyethylene glycol at various molar ratios. As a CE method. capilary electrophoresis sodium dodecyl sulfate nongel sieving (CE-SDS NGS) was performed using an uncoated capilary filled with a hydrophilic replaceable polymer network matrix. (omitted)

• Food Science and Biotechnology
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• 제18권5호
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• pp.1288-1292
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• 2009

Volatile flavor compounds were isolated from the flowers of Chrysanthemum indicum L. (gamguk) produced in Korea and China by the hydro distillation, and were analyzed by gas chromatography-mass spectrometry (GC/MS). The yield of oils from Korean and Chinese gamguk were 2.0 and 0.5%(v/w), respectively. Sixty-three volatile compounds of Korean gamguk representing 89.28% of the total peak area were tentatively identified, including 35 hydrocarbons, 12 alcohols, 6 ketones, 3 esters, 5 aldehydes, 1 oxide, and 1 miscellaneous component. Thirty-six volatile components of Chinese gamguk constituted 58.15% of the total volatile composition were tentatively characterized, consisting of 19 hydrocarbons, 7 alcohols, 2 ketones, 2 esters, 4 aldehydes, 1 oxide, and 1 miscellaneous component. The predominant components of Korean oil were ${\alpha}$-pinene, 1,8-cineol, and chrysanthenone. Whereas, camphor, ${\alpha}$-curcumene, and ${\beta}$-sesquiphellandrene were the main aroma compounds of Chinese gamguk.

• 대한화학회지
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• 제21권3호
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• pp.193-203
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• 1977

오이, 수박, 수세미, 박, 하늘수박의 다섯가지 박과식물 종자유에서 세가지 스테롤 플랙숀을 박층 크로마토그래피로 나누고 각 플랙숀의 조성을 가스크로와 가스크로에 연결된 질량분석법으로 밝혔다. 이중 4-데스메틸스테롤 플랙숀에서 ${\alpha}$-Spinasterol, ${\Delta}^{7,22,25}$-stigmastatrienol 및 ${\Delta}^{7,25}$-stigmastadienol를 단리(單離)하여 IR, NMR 및 질량분석법으로 동정(同定)하였다.

• Bulletin of the Korean Chemical Society
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• 제23권11호
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• pp.1647-1650
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• 2002

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 2002년도 추계학술발표회요약집
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• pp.383.1-383
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• 2002

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2015년도 추계학술논문요약집
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• pp.473-474
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• 2015

• Mass Spectrometry Letters
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• 제2권3호
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• pp.69-72
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• 2011

Defective synthesis of the steroid hormones by the adrenal cortex has profound effects on human development and homeostasis. Due to the time-consuming chromatography procedure combined with mass spectrometry, the matrix-assisted laser desorption ionization method coupled to the linear ion-trap tandem mass spectrometry (MALDI-LTQ-MS/MS) was developed for quantitative analysis of 10 adrenal steroids in human serum. Although MALDI-MS can be introduced for its applicability as a high-throughput screening method, it has a limitation on reproducibility within and between samples, which renders poor reproducibility for quantification. For quantitative MALDI-MS/MS analysis, the stable-isotope labeled internal standards were used and the conditions of crystallization were tested. The precision and accuracy were 3.1~35.5% and 83.8~138.5%, respectively, when a mixture of 10 mg/mL ${\alpha}$-cyano-4-hydroxycinnamic acid in 0.2% TFA of 70% acetonitrile was used as the MALDI matrix. The limit of quantification ranged from 5 to 340 ng/mL, and the linearity as a correlation coefficient was higher than 0.988 for all analytes in the calibration range. Clinical applications include quantitative analyses of patients with congenital adrenal hyperplasia. The devised MALDI-MS/MS technique could be successfully applied to diagnosis of clinical samples.

• 분석과학
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• 제17권4호
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• pp.302-307
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• 2004

환경시료중의 극미량의 악티늄계 동위원소들을 분석하기는 무척 어렵다. 이들 원소들은 개별 분리하는 작업이 필요하며, 알파분광법으로 분석한 어떤 핵종들은 검출감도도 높은 편이다. 이런 극미량의 악티늄계 동위원소들을 분석하기 위해 용매추출법이 결합된 TRU-Spec 이온교환수지와 음이온 교환수지를 사용하여 악티늄계 원소들을 분리한 후 알파분광법으로 검출하였다. 그리고 U과 Th의 검출한계를 낮추기 위해 중성자방사화분석법을 적용하였다. 중성자방사화분석법을 적용하기 위한 바탕물질로 고순도 V foil을 사용하여 검출감도를 10배 향상시킬 수 있었으며, 이 분석법을 표준시료인 NIST-4354, IAEA-368 퇴적물 시료에 적용한 결과 표준값과 10% 이내에서 잘 일치하였다.

• Mass Spectrometry Letters
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• 제4권4호
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• pp.75-78
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• 2013

A gas chromatography/mass spectrometric (GC/MS) method was developed as a candidate reference method for the accurate determination of essential fatty acids (linoleic acid, ${\alpha}$- and ${\gamma}$-linolenic acids) in food supplemental oil products. Samples were spiked with three internal standards (stearic acid-$d_{35}$, $^{13}C_{18}$-linoleic acid, and $^{13}C_{18}$-${\alpha}$-linolenic acid). Samples were then subject to saponification, derivatization for methylation, and extraction by organic solvent. For GC/MS measurement, an Agilent HP-88 column, designed for the separation of fatty acid methyl esters, was selected after comparing with other columns as it provided better separation for target analytes. Target analytes and internal standards were detected by selected ion monitoring of molecular ions of their methyl ester forms. The GC/MS method was applied for the measurement of three botanical oils in NIST SRM 3274 (borage oil, evening primrose oil, and flax oil), and measurement results agreed with the certified values. Measurement results for target analytes which have corresponding isotope-labeled analogues as internal standard were calculated based on isotope dilution mass spectrometry (IDMS) approach, and compared with results calculated by using the other two internal standards. Results from the IDMS approach and the typical internal standard approach were in good agreement within their measurement uncertainties. It proves that the developed GC/MS method can provide similar metrological quality with IDMS methods for the measurement of fatty acids in natural oil samples if a proper fatty acid is used as an internal standard.

• 방사성폐기물학회지
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• 제4권2호
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• pp.95-102
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• 2006

알파분광법에 의한 $^{237}Np$ 의 정량방법을 검토하였다. 황산염 매질에서 전류세기, 전착시간 및 유기물 첨가제 둥의 변화에 따른 $^{237}Np$ 의 전착조건을 찾은 결과 $1.2{\sim}1.5$ A에서 유기첨가제 없이 $1{\sim}1.5$ 시간 동안 전착하는 것이 효율적이었다. $^{237}Np$ 을 4.16 Bq에서 0.0264 Bq(1ng) 까지 전착한 결과 농도가 낮을수록 전착율 및 재현성이 낮아졌으며 1 ng 까지 측정이 가능하였다. 사용후 핵연료 합성용액에서 $^{237}Np$을 분리한 후 알파분광법으로 측정하여 정량한 결과 $98.8{\pm}5.1%$(n=4)의 회수율을 나타내었다. 사용후 핵연료 시료용액 중 $^{237}Np$ 을 정량하였으며 계산 값과 비교한 결과 10% 이내에서 서로 일치하였다.