• Korean Chemical Engineering Research
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• 제49권5호
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• pp.617-622
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• 2011

심해상어 간유에서 추출한 천연유지는 스쿠알렌(Squalene)과 알콕시글리세롤(Alkoxyglycerol)을 함유하고 있다. 본 연구에서는 심해상어 간유에서 피부 보호능력이 있는 스쿠알렌과 면역작용이 우수한 알콕시글리세롤을 추출하고 GC-MS 분석을 통하여 정제과정을 거친 스쿠알렌과 알콕시글리세롤의 함량 변화를 비교하고 스쿠알란과 함께 물성을 평가하였다. 스쿠알렌의 함량은 정제과정을 거친 뒤에 35% 더 증가하였으며 알콕시글리세롤의 함량은 21.9% 더 증가한 것을 볼 수 있었다. 상어간유를 알콕시글리세롤 정제과정을 통해 지방산은 61%에서 4%로 57%의 지방산이 제거된 것을 확인할 수 있었고, 지방산 중 Palmitic acid와 Oleic acid의 함량이 크게 감소된 것을 확인할 수 있었다. 상어간유에 과산화물가와 산가는 스쿠알렌 정제과정을 거친 후 크게 낮아진 것을 확인하였고 불포화 지방산이 이중결합이 많은 스쿠알렌이 스쿠알란(Squalane)보다 요도드가가 월등히 큰 것을 확인하였다.

• Natural Product Sciences
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• 제15권2호
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• pp.71-75
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• 2009

The great economic and social damage caused by unusual explosion of jellyfish population has attracted the attention of researchers. A chemical study on the bioactive components of the giant jellyfish Nemopilema nomurai led to the isolation of two new (1 and 2) and three known alkoxyglycerols (3 - 5), along with known monoglycerides (6 - 7) and fatty acids. Based on NMR and MS data, the structures of compounds 1 and 2 were elucidated as 1-O-[(Z)-tetradec-3-enyl]-sn-glycerol and 1-O-[(Z)-octadec-10-enyl]-sn-glycerol, respectively. The absolute configurations of compounds 1 - 7 were determined by comparison of specific optical rotation values with those reported. The isolated compounds were evaluated for suppressive effect on the proinflammatory mediators (NO, IL-6, and TNF-${\alpha}$) in murine macrophage cells. However, they were found inactive upto the concentration of 100 ${\mu}M$.

• 생약학회지
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• 제28권4호
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• pp.286-292
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• 1997

We studied the screening condition for immune regulatory responsor. We focused on the T-lymphocytes leer this purpose. Mouse fetal thymic organ culture (FTOC) system and flow cytometric analysis were mainly used in this experiment. Even if FTOC is carried out in vivo condition, the pattern of thymic development in the condition of FTOC is similar to that of in vivo condition. In this regard, FTOC system might be very powerful tool to screen the immune regulator, especially concerning on T cells. To establish the optimum condition of FTOC to screen the Immune regulator, we focused on the optimum amount of dose and culture period. The cell number and surface antigens on T cells were also analysed by using hemacytometer and flow cytometer. To monitor the differentiation event, anti-CD3, anti-CD4 and anti-CD8 antibodies were used. Alkoxyglycerol and Phellodendri Cortex were used fur positive and negative control, respectively. Astragalus membranceus was used as test sample. From our analysis, we reached to conclusions that the best dose of extract is $50\;{\mu}g/ml$ of culture medium, the best culture period is for 9 days, and ethanol used as solvent has no toxicity to FTOC.