Browse > Article
http://dx.doi.org/10.9713/kcer.2011.49.5.617

Physical Property and Extraction of Squalene and Alkoxyglycerol from Shark Liver Oil  

Lee, Su Il (Department of Chemical Engineering, Inha University)
Heo, Hyo Jung (Department of Chemical Engineering, Inha University)
Row, Kyung Ho (Department of Chemical Engineering, Inha University)
Publication Information
Korean Chemical Engineering Research / v.49, no.5, 2011 , pp. 617-622 More about this Journal
Abstract
A simple method has been successfully applied to extract squalene and its byproducts alkoxyglycerol from deep-sea shark liver oil. GC-MS was used to determine the extraction amount of the squalene and alkoxyglycerol, and the content of them in rough product and refined product were compared. The physical property of squalene was identified by measuring the pH value, peroxide value and iodine value. Under optimum extraction conditions, the amount of squalene and alkoxyglycerol increased 35.0% and 21.9%, respectively, while the amount of fatty acid decreased from 61% to 4%, especially, the amount of palmitic acid and oleic acid remarkably decreased. Large amount of peroxide and acid were removed from shark liver oil after refining process. Because squalene contains lots of double bond, so the value of iodine is much higher than squalane.
Keywords
Shark Liver Oil; Squalene; Alkoxyglycerol; Separation; Physical Property;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim, J. S., Yoon, J. S., Choi, Y. B., Choi, K. P., Kim, J. S. and Chung, S. M., "Healing Effects of Squalene on the Epifermis in Burned Mouse," Kor. J. Electron Microscopy, 29(2), 163-175 (1999).
2 Kim, J. S. and Yoon, J. S., "Effect of Squalene on Mouse Liver Toxicity with Cadmium," Kor. J. Electron. Microscopy., 30(2), 141-152(2000).
3 Yamawaki, M., Azuma, I., Saiki, I., Uemiya, M., Aoki, O., Ennyu, K. and Yamamura, Y., "Antitumor Activity of Squalene Treated Cell Wall Skeleton of Nocardiarubra in Mice," Gann., 69(5), 619-626(1978).
4 Bonney, R. J., Becker, J. E., Walker, P. R. and Potter, V. R., "Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis," In Vitro., 9(6), 399-413(1974).   DOI   ScienceOn
5 Moldeus, P., Hogberg, J. and Orrenius, S. "Isolation and Use of Liver Cells," Meth. Enzymol., 52, 66-72(1978).
6 Hallgren, B. and Larsson, S., "The Glyceryl Ethers in Man and Cow," J. Lipid. Res., 3, 39-43(1962).
7 Hallgren, B. and Larsson, S., "The Glyceryl Ethers in the Liver Oils of Elasmobranch Fish," J. Lipid. Res. 3, 31-38(1962).
8 Blomstrand, R. and Ahrens, E. H. Jr., "Absorption of Chimyl Alcohol in Man," Proc. Soc. Exp. Biol. Med., 100, 802-805(1959).   DOI
9 Brohult, A., "Alkoxyglycerol-esters in Irradiation Treatment," Advances in Radiobiology(Proc. of the 5:th Int. Conf. on Radiobiology, Stockholm), 2, 241-248(August, 1956).
10 Maulik, N., Tosaki, A., Engelman, R. M., Cordis, G. A. and Das, D. K., "Myocardial Salvage by 1-o-hexadecyl-sn-glycerol: Possible Role of Peroxisomal Dysfunction in Ischemia Reperfusion Injury," J. Cardiovas. Pharmacol., 24, 486-492(1994).   DOI   ScienceOn
11 Liu, G. C. K., Ahrens, E. H. Jr., Schreibman, P. H. and Crouse, J. R., "Measurement of Squalene in Human Tissue and Plasma," J. Lipid. Res., 17, 38-45(1976).
12 Sabeena Farvin, K. H., Anandan, R., Kumar, S. H., Shiny, K. S., Sankar, T. V. and Thankappan, T. K., "Effect of Squalene on Tissue Defense System in Isoproterenol-induced Myocardial Infarction in Rats," Pharmacological Research, 50(3), 231-236(2004).   DOI   ScienceOn
13 Kohno, Y., Egawa, Y., Itoh, S. Nagoka, S., Takahashi, M. and Mukai, K. "Kinetic Study of Quenchin Reaction of Singlet Oxygen and Scavenging Reaction of Free Radical by Squalene in n- Butanol," Biochim. Biophys. Acta., 1256(1), 52-56(1995).   DOI   ScienceOn
14 Budiarso, I. T., "Fish Oil Versus Olive Oil," Lancet, 336, 1313-1314(1990).   DOI   ScienceOn