• YAKHAK HOEJI
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• v.37 no.2
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• pp.187-192
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• 1993

Approximately 1% of intestinal bacteria of human and rats was alkalotolerant. Among these bacteria of human, bacteria producing $\beta$-glucosidase, $\beta$-glucuronidase and sulfotransferase were 40%, 4% and 0%, respectively. Among alkalotolerant intestinal bacteria of rats, bacteria producing, these enzymes were 70%, 8% and 0%, respectively. $\beta$-Glucosidase and $\beta$-glucuronidase of alkalotolerant intestinal bacteria of human and rat were induced by the medium of high pH: these enzymes activities were increased by elevating pH of the medium, but the growths were not changed. The enzyme activities at the medium of pH 7 were about ten-fold higher than those at the medium of pH 6.

• Journal of Soil and Groundwater Environment
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• v.17 no.1
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• pp.47-54
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• 2012

When quicklime is added into soil for various purposes, abrupt changes in soil chemistry may affect essential ecological functions played by indigenous bacterial communities in soil. The magnitude of influence was estimated by observing changes in abundance and diversity of soil bacteria after quicklime treatment. When several soil samples were treated up to 20% (w/w) quicklime, plate count of viable cells ranged $10^2{\sim}10^3$ CFU $g^{-1}$, showing a reduction of more than $10^4$ times from viable counts of the untreated sample. Diversity of the bacterial isolates that survived after quicklime treatment was analyzed by conducting $GTG_5$ rep-PCR fingerprinting. There were only two types of fingerprints common to both 5% and 20% quicklime samples, implying that bacteria surviving at different strength of quicklime treatment differed depending on their tolerance to quicklime-treated condition. Isolates surviving the quicklime treatments were further characterized by Gram staining and endospore staining. All isolates were found to be Gram positive bacteria, and 85.4% of them displayed endospores state. In conclusion, most bacteria surviving quicklime treatment appear to be endospores. This finding suggests that most of ecological functions of bacteria in soil are lost with quicklime treatment.