• Korean Journal of Medicinal Crop Science
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• v.12 no.5
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• pp.428-435
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• 2004

Alkaloid producing species of plants have long been a major component of the medicinal social and magico-religious aspects of human culture. A diverse array of biological activities has been attributed to different alkaloids including numerous members of benzylisoquinoline family of alkaloids. For biotechnological approaches of alkaloid biosynthesis in poppy family, plant regeneration protocol through somatic embryogenesis or shoot organogenesis is a first step. This paper describes the methods and applications of plant regeneration of poppy family.

• Archives of Pharmacal Research
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• v.12 no.4
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• pp.306-309
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• 1989

Five new sesquiterpene alkaloids, euojaponine-D, -F, -G, -J, and -K were isolated from the root bark of Euonymus japonica Thunb, and their structures were elucidated by various spectroscopic methods.

• Journal of Plant Biotechnology
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• v.6 no.2
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• pp.125-130
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• 2004

Callus and cell suspension cultures of Eurycoma longifolia Jack could be an alternative supply of 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one. The callus tissues were initiated from leaves of different trees. The friable calli were used for the preparation of the cell suspension cultures of E. longifolia. The leaf explant of tree Eu-9 produced the most callus and also induced high cell biomass in the cell suspension culture, but it produced low quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. The leaf explant from tree Eu-8 produced low quantity of callus and cell biomass, but produced the highest quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. Optimum production of cell biomass was obtained on cell culture medium with pH 5.75 prior to autoclaving, but high alkaloids content could be induced in culture medium in acidic condition with pH 4.75 and 5.25 prior to autoclaving.

• Journal of the Korean Society of Tobacco Science
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• v.25 no.2
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• pp.87-94
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• 2003

Recently, many researches for plant alkaloids, one of the largest groups of natural products, are reported because of their various pharmacological activity. This study was carried out to clone putrescine N-methyltransferase (PMT) gene which is a key enzyme in diverting polyamine metabolism towards the biosynthesis of nicotine and related alkaloids from Burley tobacco. To induce expression of PMT gene in tobacco plant, the floral meristem was removed and then mRNA was purified from root. cDNA encoding PMT gene was isolated by RT PCR and cloned. Three different groups of clones were screened by PCR and restriction enzyme digestion analysis and were characterized. The data of these screening revealed that three types of PMT are present in Burley tobacco. Comparison of the nucleotide sequence of this three genes encoding putative PMT with those of other tobaccos revealed that two types of PMT are newly discovered from Nicotiana tabacum cv. Br21 tobacco and they were same as PMT2, PMT3 of N. tabacum cv. Xanthi.

• Korean Journal of Pharmacognosy
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• v.20 no.2
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• pp.83-87
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• 1989

The alkaloids of thirty five Korean plants which belong to Rhamnaceae, Sterculiaceae, Celastracea, Urticaceae and Rubiaceae were screened. Plant was extracted and solvent fractionated to yield ether soluble alkaloidal fraction and butanol soluble fraction. Each fraction was subjected to alkaloidal test by Dragendorff's reaction. The result showed eighteen plants strong positive, fourteen weak and six negative to alkaloidal test.

• Korean Journal of Pharmacognosy
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• v.49 no.2
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• pp.108-112
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• 2018

Four quinolone alkaloids, 2-heptyl-4-quinolone (1), 2-nonyl-4-quinolone (2), 2-undecyl-4-quinolone (3), and 2-undecen-1'-yl-4-quinolone (4), together with two nitrogen derived benzoic acid derivatives, N-acetylanthranilic acid (5) and o-acetamidobenzamide (6) have been isolated from the Arctic bacterial strain, Pseudomonas aeruginosa. The structures of the compounds were determined by 1D and 2D NMR, and MS experiments, as well as by comparison of their data with published values. To the best of our knowledge, compounds 3-6 were isolated for the first time from P. aeruginosa.

• Journal of the Korean Society of Tobacco Science
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• v.5 no.1
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• pp.35-42
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• 1983

This study was conducted to know changes in chemical components and some enzyme activity on tobacco leaves of NC2326 and Hyangchio (Nicotiana tabacum L. ) by different curing methods. The activity of $\alpha$, $\beta$-amylase and invertase of flue-cured tobacco variety NC2326 were higher than that of sun-cured variety Hyangchio. And the peak of all of enzyme activity in both varieties showed at about 60 hours (at color fixing stage) and 6 days (yellowing stage) after start of flue-curing and sun-curing, respectively. The peak time of reducing sugar content were same with the maximum enzyme activity during curing process in both varieties. During both curing process, the content of starch was greatly reduced and there was a corresponding increase in reducing sugars. Increase in total alkaloids content continued during flue-curing process in both varieties, but during sun-curing process total alkaloids content of NC 2326 were Increased whereas those of Hyangchio were decreased markedly.

• Journal of the Korean Chemical Society
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• v.47 no.6
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• pp.597-600
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• 2003

Cinchona alkaloids (1-4) were used as chiral ligands for the enantioselective Reformatsky reactions. The order of addition of the Reformatsky reagent to ligands was the important factors for the chiral induction. The ratio of Reformatsky reagent to ligand and aldehyde was also effected on the reaction yields.

• Journal of Plant Biology
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• v.36 no.3
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• pp.225-231
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• 1993

Transformed hairy roots of Scopolia parviflora, producing tropane alkaloids and native to Korea, were obtained following infection of rhizome segments with Agrobacterium rhizogenes A4. Each root tip induced from inoculum sites excised and cultured in MS agar or liquid medium. About seventy of hairy root clones were established. Among them, several fast growing hairy root clones were examined for alkaliod content. Two dimensional TLC analysis showed that the tropane alkaloid pattern of hairy root was more complicated than that in the rhizome of mother plant. On the other hand, some hairy root clones did not produce scopolamine and hyoscyamine. In HPLC analysis, some hairy root clones yield higher levels of scopolamine and hyoscyamine than those of mother plant rhizome which used for infection. Scopolamine and hyoscyamine were identified by comparison of their retention times and of their spectra data with those of authentic compounds.

• Journal of Ginseng Research
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• v.1 no.1
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• pp.29-32
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• 1976

Succinate dehydrogenase was activated by ethyl acetate extract from the methanol extract of white ginseng previously treated with petroleum ether and ethyl ether to remove all highly nonpolar components, and the residual aqueous solution from the ethyl acetate extraction. Also, all of the extracted fractions of red ginseng except the ether extract gave positive results. On the contrary to some suggestions by other workers that alkaloids of ginseng may enhance the succinate degydrogenase activity, our results show that the alkaloids may have exhibited some inhibitory eject on this enzyme.