• Journal of Aquaculture
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• v.15 no.2
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• pp.79-86
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• 2002

This study is the first attempt to understand the feeding physiology of a sea-urchin larva on a red-tide dinoflagellate. Fifteen day old larvae of S. intermedius capture C. polykrikoides cells by localized reversal of ciliary beats. No failure to transporte the algal cells from theciliated band to mouth and no rejection at the mouth suggest that C. polykrikoides has no feeding deterrence to S. intermedius larvae. The trend obtained for the clearance rate of S. intermedius larvae is similar to that of other sea urchin larvae. Thus, the clearance rate decreased as the algal concentration increased. Maximum clearance rate of S. intermedius on C. polykrikoides was 17.7 $\mu l$/larva/hr. Ingestion rate rapidly increased at lower algal concentrations and saturated at higher concentrations. There was no inhibition in ingestion rate at the highest prey concentration of ca. 3000 cells/ml. Maximum ingestion rate of S. intermedius on C. polykrikoides was 131 ngC/larva/d, which is higher than that reported for the larvae of the mussel Mytilus gal-lotrovincialis, but lower than that of the ciliate Strombidinopsis sp. The grazing rate, calculated by combining the field data on algal abundances with experimental data on ingestion rate, suggests that due to its low abundance, sea urchin Iarva has no significant grazing impact on C. polykrikoides population.

• ALGAE
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• v.35 no.3
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• pp.225-236
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• 2020

Gymnodinium smaydae is a newly described mixotrophic dinoflagellate that feeds on only Heterocapsa spp. and Scrippsiella acuminata among 19 tested algal prey. It is one of the fastest growing dinoflagellates when feeding, but does not grow well without prey. To investigate its spatial-temporal distributions in Korean waters, we quantified its abundance in water samples that were seasonally collected from 28 stations along the Korean Peninsula from April 2015 to October 2018, using quantitative real-time polymerase chain reactions. This dinoflagellate had a wide distribution, as reflected by the detection of G. smaydae cells at 23 of the sampling stations. However, this distribution had a strong seasonality; it was detected at 21 stations in the summer and only one station in winter. The abundance of G. smaydae was significantly and positively correlated with chlorophyll a concentration as well as with water temperature. However, there were no significant correlations between the abundance of G. smaydae and salinity, concentrations of nutrients, or dissolved oxygen concentration. During the study period, G. smaydae was present when water temperatures were 7.6-28.0℃, salinities were 9.6-34.1, concentrations of NO₃ were not detectable-106.0 μM, and concentrations of PO₄ were not detectable-3.4 μM. The highest abundance of G. smaydae was 18.5 cells mL^-1 in the coastal waters of Jinhae in July 2017 when the chlorophyll a concentration was 127 mg m^-3 and water temperature was 23.8℃. Therefore, the spatial-temporal distribution of G. smaydae in Korean coastal waters may be affected by chlorophyll a concentration and water temperature.

• Journal of Aquaculture
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• v.11 no.3
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• pp.401-419
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• 1998

The structure of benthic algal flora and feeding behavior of sea urchins (Anthocidaris crassispina and Hemicentrotus pulcherrimus), were investigated from the eight different locations along the coast of Cheju Island, Korea. Biomass distribution and dominance of the algal communities were studied in relation to the gut contents and population parameters of the sea urchins. As a result, although the largest algal biomass and biological characteristics of sea urchins, such as test diameter, test hight, body weight, gonad weight, etc. This inhdicated that sea urchins would not be dependednt sorely on the abundance of algal foods, and they might feed on other food items. In order to know such a trend with regard to food selectivity, the diets consumed by sea urchins were analyzed, and it was found that they were composed of benthic and drifted algae, various animal components (sponges, bryozoans, crustaceans, and various unidentified capsulated eggs, etc.) and biets turned out to be smaller than might be expected, and it is postulated that sea urchins were not likely to be the major grazing animals for the formation of algal bed and also canopy deterioration, so-called "white incrustation."

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.4
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• pp.387-394
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• 2009

Marine algal flora and community structure were examined seasonally at four study sites around Taean Power Plant, Korea from Jan. to Nov. 2006. A total of 73 algae species (12 green, 9 brown, 52 red) and 1 marine plant were identified. The number of species was maximal at the power plant Discharge (57 species) site followed by Hakampo (46 species), Intake (28 species) and Breakwater (15 species) sites during the study period. The average biomass in dry weight varied from 13.12g/m2 at Intake to 69.60g/m2 at Hakampo. Dominant and sub dominant species in terms of biomass were Gelidium divaricatum - Ulva pertusa at Intake, Chondria crassicaulis - Ulva pertusa at Discharge, Corallin a pilulifera - Chondrus ocellatus at Breakwater, and Corallina pilulifera - Sargassum thunbergii at Hakampo. Species richness of warm tolerant and green algae were greater at Discharge site than Hakampo, showing similar species richness. However, community indices were not distinguishable between Discharge and other study sites. In conclusion, species richness and biomass of seaweeds were greater at Discharge site compared to intake and breakwater sites, and the abundance of warm tolerant and green algal species were higher than Hakampo.

• KCID journal
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• v.18 no.1
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• pp.33-43
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• 2011

Jundae reservoir has basin area of 234ha, average depth of 3.77m and total storage of $619{\times}10^3m^3$, and is located in Dangin-gun, Chungcheongnam-do. The water quality of Jundae reservoir exceeded the IV grade of water quality standard as available for irrigation water in COD, TN, TP, Chl-a. COD and Chl-a were higher in spring season, because the algal bloom by phytoplankton increased. And the algal blooms in October by inflow non-point pollution during summer rainy season. The most dominant zooplankton was rotifers during study period at all stations. Dominant species were Keratella cochlearis, Polyarthra spp., and Trichocerca spp. We successfully established 2 isolated clone cultures as predator. One is Rotifer, Euchlanis sp. and another is cladocerans, Bosmina sp. To test the removal rate of 2 cultures against Microcystis aeruginosa, we inoculated Euchlanis sp. and Bosmina sp. separately when the abundance reached at $1.0{\times}10^6$cells/ml. Euchlanis sp. removed M. aeruginosa around 98.9% and Bosmina sp. removed it around 98.4%. They are useful grazers for controling algae blooms, Euchlanis sp. and Bosmina sp. feeding on M. aeruginosa highly.

• ALGAE
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• v.30 no.2
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• pp.139-146
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• 2015

Urchin barrens have been a major issue of rocky coastal ecosystems in temperate regions. In South Korea, the east coast and Jeju Island have especially been a focus because the area of barren ground increases in spite of continual efforts to install artificial reefs. This study approached the urchin barrens issue in South Korea, by focusing on a correlational analysis of urchin and macroalgal abundance. Urchin density and algal species coverage were obtained using a quadrat image analysis. Subtidal sites were then classified into three groups according to the average densities of urchins to evaluate the characterization of the macroalgal community: no urchin (NU) zone; transition (TR) zone, $4inds.\;m^{-2};$ and urchin (UR) zone, ${\geq}8inds.\;m^{-2}$. The average urchin density in the study site was $4.7inds.\;m^{-2}$ and 57 macroalgal species were found in the study site. From the NU zone to UR zone, total species number, species diversity index and evenness gradually decreased, whereas the dominance index increased. The algae species with negative correlations were Grateloupia divaricata, Polysiphonia morrowii, Chondracanthus intermedius, Delesseria violacea, Desmarestia viridis; and those with positive correlations were the crustose corallines, Sargassum horneri. Other species were not significantly correlated with urchin density. The significant correlations indicate that the abundance of some macroalgal species is proportionally regulated by sea urchin density. This study also shows how macroalgal vegetation changes in response to an urchin's density gradient in a natural condition; and there is a TR zone that existed with respect to an intermediate level of algal abundance.

• ALGAE
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• v.37 no.4
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• pp.281-291
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• 2022

Quantifying the abundance of Chattonella species is necessary to effectively manage the threats from ichthyotoxic raphidophytes, which can cause large-scale mortality of aquacultured fish in temperate waters. The identification and cell counting of Chattonella species have been conducted primarily on living cells without fixation by light microscopy because routine fixatives do not retain their morphological features. Species belonging to the Chattonella marina complex, including C. marina and C. marina var. ovata, had high genetic similarities and the lack of clear morphological delimitations between the species. To estimate the abundance of C. marina complex in marine plankton samples, we developed a protocol based on the droplet digital polymerase chain reaction (ddPCR) assay, with C. marina complex-specific primers targeting the internal transcribed spacer (ITS) region of the rDNA. Cell abundance of the C. marina complex can be determined using the ITS copy number per cell, ranging from 25 ± 1 for C. marina to 112 ± 7 for C. marina var. ovata. There were no significant differences in ITS copies estimated by the ddPCR assay between environmental DNA samples from various localities spiked with the same number of cells of culture strains. This approach can be employed to improve the monitoring efficiency of various marine protists and to support the implementation of management for harmful algal blooms, which are difficult to analyze using microscopy alone.

• Korean Journal of Environmental Biology
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• v.37 no.3
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• pp.433-445
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• 2019

This study was designed to develop simple tools to easily and efficiently predict the occurrence of algal bloom in agricultural lakes. Physicochemical water quality parameters were examined to reflect the phytoplankton productivity in 182 samples collected from 15 agricultural lakes from April to October 2018. Total phytoplankton abundance was significantly correlated with chlorophyll-a (Chl-a) (r=0.666) and Secchi depth (SD) (r= -0.351). The abundances of cyanobacteria and harmful cyanobacteria were also correlated with Chl-a (r=0.664, r=0.353) and SD (r= -0.340, r= -0.338), respectively, but not with total nitrogen (TN) and total phosphorus (TP). The Chl-a concentration was correlated with SD (r= -0.434), showing a higher similarity than phytoplankton abundance. Therefore, Chl-a and SD were selected as diagnostic factors for algal bloom prediction, instead of analyzing the standing crop of harmful cyanobacteria used in algae alarm systems. Specifically, accurate diagnoses were made using realtime SD measurements. The algal bloom diagnostic tool is an inverse cone-shaped container with an algal bloom diagnosis chart that modified SD and turbidity measurement methods. Lake water was collected to observe the number of rings visible in the container or the number indicated in each ring, depending on the degree of algal bloom,and to determine the final stage of algal blooming by comparison to the colorimetric level on the diagnosis chart. For an accurate diagnosis, we presented 4-step diagnostic criteria based on the concentration of Chl-a and the number of rings and a fan-shaped algal bloom diagnosis chart with Hexa code names. This tool eliminated the variables and errors of previous methods and the results were easily interpreted. This study is expected to facilitate the diagnosis of algal bloom in agricultural lakes and the establishment of an efficient algal bloom management plan.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.7
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• pp.1047-1053
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• 2006

The current study was designed to determine the effects of dietary docosahexaenoic acid (DHA) on fatty acid deposition in egg yolk and various tissues of laying Tsaiya ducks, and on the mRNA concentrations of hepatic lipogenesis-related transcription factors. Thirty laying ducks were randomly assigned to three treatments with diets based on corn-soybean meal (ME: 2803 kcal/kg; CP: 17.1%; Ca: 3.4%) supplemented with 0% (control diet), 0.5% or 2% algal DHA oil. The DHA content in egg yolks of the ducks was elevated significantly (p<0.01) with the supplementation of dietary DHA. The DHA percentage of the total fatty acids in the egg yolk of laying ducks was 0.5%, 1.3% and 3.4% for 0%, 0.5% and 2% algal DHA oil treatments, respectively, for the $1^{st}$ week, and 0.5%, 1.5% and 3.3% for the $2^{nd}$ week. Therefore, algal DHA oil can be utilized by laying Tsaiya ducks to enhance the egg-yolk DHA content. The concentrations of triacylglycerol (TG) and cholesterol in plasma of laying Tsaiya ducks were not affected by dietary DHA treatments (p>0.05). The DHA concentration in plasma, liver, and skeletal muscle was increased with the addition of dietary algal DHA oil (p<0.05). The mRNA abundance of sterol regulatory element binding protein 1 (SREBP1) and SREBP2 in the livers of laying Tsaiya ducks was not affected by dietary DHA, suggesting that the expression of these transcription factors is tightly controlled and not sensitive to DHA treatments.

• Korean Journal of Ecology and Environment
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• v.41 no.2
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• pp.137-143
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• 2008

The abundance and community structure of epilithic diatoms grown on different substrata were investigated in two first-order streams located in a limestone and granite area, north of the Suzuka Mountains in Central Japan. Experiments were conducted as follows: limestone and granite without algae were submerged in their own streambed or another stream station and incubated for seven weeks, while limestone and granite with algae were transferred to another stream station and incubated at the same time. The diatom biomass was consistently high in the lime-stone station experiments compared to those at the granite station. In addition, there was more diatom biomass on granite substrata than on the limestone substrata at both stations. The present results suggested that the difference in water chemistry including the major nutrient concentrations was the limiting factor for algal growth in these two streams; however, when the water chemistry was the same in each stream, the difference in substratum characteristics became the important factor affecting the diatom abundance. The diatom community grown on the transferred substrata with and without algae became similar to those grown on the original substrata in each stream during the incubation period. It was suggested that the effect of the substrata characteristics on the diatom community structure was rather small.