• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.31 no.5
• /
• pp.767-773
• /
• 1998

In this study, we have investigated the distributions and killing effects of marine bacteria that tend to kill the red tide microalgae, C. polykikoides in the area of Masan bay from June to October, 1996. To summarize, C. polykikoides killing bacteria were detected at $10^2$ to $10^3$ cells/ml of seawater samples during the survey period, and the bloom was observed in September by containing $4.8\times10^3$cells/ml. It appears however that the number of these bacteria is decreased ($2.0\times10^2$cells/ml) in October, A total of 110 strains were isolated from seawater samples and seawater filtrate (pore size, 0.8 $\mu$m)-containing mixed culture of C. polykikoides in which the mixed culture was grown in f/2 medium. As results we have successfully isolated Micrococcus sp. LG-1 which decreased to less than 10cells/ml within 6days and 5days sfter inoculation of Micrococcus sp. LG-1 into the la9 and logarithmic growth phases of C. polykrikoides respectively. Therefore, it appears that inoculation of Micrococcus sp. LG-1 against the logarithmic C. polykrikoides is more effective than the lag growth phase, (n addition, the killing effects were increased in accordance with bacterial cell densities inoculated in a dose dependent manner. Especially, the filtrate of kitling bacterium culture (nore size, 0.2 $\mu$m) revealed a dramatic effect in which C. polykrikoides were decreased to less than 10 cells/mf of culture within 1 hr, 1,5 hrs, 1,5 hrs, 3.5 hrs. and 5,5 hrs after inoculations of the culture filtrate with concentration of $30\%,\;20\%,\;10\%,\;5\%$ and $2.5\%$, respectively. Moreover Micrococcus sp. LG-1 showed a selective specificity against C. polykrikoides and any other killing effects of Micrococcus sp. LG-1 were not observed against Alexandrium tamarense, Prorocentrum micans, Scrippsiella trochoidea. ana Gymnodinium sanguineum.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.31 no.6
• /
• pp.852-858
• /
• 1998

We have isolated a bacterial strain that tends to kill P. micans from the mixed culture of p. minns plus seawater filtrate (poresize, 0.8 $\mu$m) collected at Masan bay in July 1996, in which the mixed culture grown in the f/2 medium. According to the experimental results of the isolated bacterium such as fatty acids analysis, morphological and biochemical characteristic tests, the strain was supposed to be a Pseudomonas and then it was named as Pseudomonas sp. LG-2. The killing effect of Pseudomonas sp. LG-2 against P. micans was proportionally increased with the concentrations of culture filtrate (pore size, 0.8 $\mu$m) is well as with the number of bacterium inoculated. In the mixed culture inoculated with $1.3\times10^6$ cells/ml of Pseudomonas sp. LG-2, the number of P. micans (2,000 cells/ml) was gradually decreased and then killed below 100 cells/ml within 7 days. In addition, the culture filtrate with $30\%$ of final concentration revealed a significant killing effect against P. micans around 3 days after culture. In the relationship between killing effects and growth stage of Pseudomonas sp. LG-2, the culture filtrate at lag phase has little effects on P. micans. In constant, the culture filtrate at mid-log phase showed the killing effect by decreasing P. micans to 112 in number within 5 days. In particular, the culture filtrate at stationary phase showed a significant killing effect against P. micans in which the majority of it was killed after 3 day culture. The species specificity of killing effects of Pseudomonas sp. LG-2 against 5 species of dinoflagellate was only found in P. micans and Scrippsiella trochoidea.

• Journal of Life Science
• /
• v.14 no.4
• /
• pp.589-592
• /
• 2004

The growth response of the fish-killing dinoflagellate Cochlodinium polykrikoides was studied in cultures, using the treatment of Ceratium extracts by a methanol, a water-soluble, and a cell-free medium. The cell-free medium had the most increasing on the growth of C. polykrikoides cultures, enriched with $\geq$ 25% Ceratium, whereas the methanol and water-souble fractions did not affect the growth of C. polykrikoides exposed to even higher concentration. In particular, the cell-free medium also increased the growth of Gyrodinium impudicum and Chaetoceros sp., similar species to C. polykrikoides. In contrast to C. polykrikoides, G. impudicum and Chaetoceros sp., the growth of Alexandrium tamarense was inhibited significantly, and there was no great effect on the growth of Prorocentrum minimum. These results imply that Ceratium extracts may play an important role in the stimulatory effect of C. polykrikoides, and they have to affect the interaction between C. polykrikoides and Ceratium when co-existing.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.10 no.1
• /
• pp.79-91
• /
• 2005

In order to understand growth characteristics of ten major species of microalgae responsible for frequent harmful algal blooms in Korean coastal waters, the growth rates of the isolates were examined in relation with the impacts of water temperature, salinity and irradiance. In addition, their bloom events since 1990 as well as monthly abundance of vegetative cells were analyzed. Heterocapsa triquetra, Eutreptiella gymnastica and Alexandrium tamarense were considered as relatively mid temperature adapted species in that growth rates were comparatively high at low water temperatures of $10{\sim}16^{\circ}C$ and drastically decreased at above $22^{\circ}C$. Prorocentrum micans and Pyramimonas sp. were categorized as relatively high temperature adapted species by showing comparatively better growths at high water temperatures above $25^{\circ}C$. Akashiwo sanguinea, Heterosigma akashiwo, Prorocentrum minimum and Scrippsiella trochoidea were eurythermal species with relative high growth rates in a broad ranges of water temperature, $16{\sim}25^{\circ}C$ were slightly halophobic, showing better growths at low salinities of $10{\sim}30$ psu than at above 35 psu. H. akashiwo, P. minimum and H. triquetra were euryhaline species with remarkable growths in a broad ranges of salinity, 15-40 psu. Frequent algal blooms by these three species at extremely low salinities below 25 psu after rainfall were attributed to their euryhaline and slightly halophobic physiological characteristics. Growth rates of H. akashiwo, P. minimum and Pyraminonas sp. increased with the increase of irradiance within the experimental ranges of $2{\sim}150\;{\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$. However, A. sanguinea, A. tamarense and H. triquetra showed better growths at comparatively low irradiance of $50{\sim}100\;{\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$ and drastic decreases in growth rates above $150\;{\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$ of irradiance. Overall, relatively high temperature adapted species make blooms frequently in high water temperature season with strong natural irradiance, and relatively low temperature adapted species grow better at low water temperature with relatively weak natural irradiance.

• Journal of Life Science
• /
• v.11 no.2
• /
• pp.74-80
• /
• 2001

The nucleotide sequences of the internal transcribed spacer regions (ITS1 and ITS2) of ribosomal DNA (rDNA), and the 5.85 rRNA gene, have been determined for 13 strains of dinoflagellates in order to analyze the phylo-genetic relationship. The DNA sequences contained considerable variation in the ITS regions, but little in the 5.85 rDNA. In addition, the ITS1 was more variable than the ITS2 in all species examined. The nucleotide length of this region varied from 519 bp to 596 bp depending on the taxa. The investigated taxa were divided into three large groups based on the ITS length, i. e., a group with short ITS region (A. fraterculus and Alexandrium sp.), a with ITS region group (P. micans, P. minimum and P. triestinum) and a with ITS region group (G. impudicum, C. polykrikoides, G. sanguineum, G. catenatum and H. triquetra). The relationship between nucleotide length of ITS1 and that of ITS2 was negative, whereas G+C content and nucleotide length showed positive correlation. In phylogenetic analyses producing NJ trees, the topology was similar cluster and clearly divided the taxa into three groups based on 5.8S rDNA that were similar to those based on morphological characteristics. In particular, G. impudicum was more closely related to G. catenatum than to C. polykrikoides using phylogenetic analysis. From this study, we chew that the length of ITS region contributes to discriminate Korean harmful algal species and ITS analysis is a useful method for resolving the systematic relationships of dinoflagellates.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.26 no.5
• /
• pp.523-530
• /
• 2020

Based on the results of harmful algal blooms (HABs) monitoring by the National Institute of Fisheries Science and local governments, the effects of changes in the marine environment on HABs are described. Since the beginning of HABs monitoring in 1972, they continued to increase from the 1980s to the 1990s. After the largest number of HAB incidents (109) in 1998; the trend declined until the 2010s. Most HABs in the 1970s were caused by diatoms. In the 1980s, coastal dinoflagellates caused HABs; Cochlodinium polykrikoides blooms have been occurring continuously since 1993. There are three HAB species that cause damage to fisheries in Korea. The high-density bloom of Karenia mikimotoi caused mass mortality in shellfish in Jinhae Bay in 1981. Karenia sp. blooms occurring around Tongyeong in 1992 killed aquaculture fish. Since the occurrence of the largest fisheries damage of KRW 76.6 billion in 1995 caused by C. polykrikoides blooms, they have been occurring continuously. The concentration of nutrients in coastal waters was the highest in the 1980s and has declined since the mid-1990s. This reduction in nutrient concentration is a good explanation for the decreasing number of HABs. Since 2016, a summer high water temperature of 30℃ or more has appeared, and the range and scale of C. polykrikoides blooms have been greatly reduced. In 2016, K. mikimotoi blooms occurred around Wando, Jangheung and Goheung and small scale blooms of C. polykrikoides occurred around Yeosu. There were no C. polykrikoides blooms in 2017; however, Alexandrium affine blooms occurred from Yeosu to Tongyeong. There was a small-scale blooms of C. polykrikoides in 2018 compared to those in the previous years. Our results show that reduction in nutrients and the high water temperature owing to climate change are a good explanation for variation in HABs in Korean coastal waters.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.8 no.2
• /
• pp.111-120
• /
• 2003

To describe dinoflagellate cysts from Gwangyang Bay, surface sediment samples were collected at 20 sites by the TFO core sampler on 24 August 2001, in coupled with a phytoplankton investigation by surface seawater sampling. More than 17 genera, 36 species of dinoflagellate cysts were Identified from the sediment samples of Gwangyang Bay, consisting of 14 species of gonyaulacoid, 14 species of protoperidinioid, 3 species of diplopsalid, 2 species of gymnodinioid, 1 species of tuberculodinioid and calciodinellid, respectively. Cyst concentrations in Gwangyang Bay varied from 115 to 2,188 cysts/g, and generally increased toward a western part of the study area. The highest cyst concentration was observed at St. 11 located in the northwestern region with 11 genera and 19 species(2,188 cysts/g), while the lowest value with 6 genera and 9 species(115 cysts/g) was observed at St. 3 located in the center of the study area. The predominant dinoflagellate cyst was Spiniferites bulloideus, followed by Alexandrium sp., Brigantedinium simplex and S. delicatus. The motile forms of eight dinoflagellate cysts recorded in the sediment samples were also observed in the seawater: Polykrikos swartzii/kofoidii complex, Scripssiella trochoidea, Protoperidinium claudicans(cyst name: Votadinium spinosum), P. pentagonum(: Trinovantedinium capitatum capitatum), P. conicum(: Selenopemphix quanta), P. leonis(: Quinquecuspis concretum), P. conicoides(: Brigantedinium simplex), Gonyaulax spp.(: Spiniferites spp.). In this study, heterotrophic dinoflagellate cysts show the highest concentration at St. 6 where the highest density of diatoms simultaneously observed from surface water sample. This result suggests that the grazing of heterotrophic dinoflagellates on the diatoms in high concentration caused the higher concentration of heterotrophic dinoflagellate cysts.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.7 no.3
• /
• pp.129-139
• /
• 2002

We investigated the outbreak of red tides dominated by harmful dinoflagellates from August to November 1999 in the coastal waters off the southern Saemankeum areas where a huge red tide dominated by Cochlodinium polykrikoides had been first observed in 1998. We took water samples from 2~5 depths of 4 stations (with 3-4 additional stations during red tides) in this study period and then measured the abundance of phytoplankton, water temperature, salinity, and the concentrations of nutrients. In the study period harmful dinoflagellates Alexandrium tamarense, C. polykrikoides, Gymnodnium catenatum, Gyrodinium aureolum, Gymnodnium impudicum were present, and of these G. aureolum and C. polykrikoides formed red tide patches on September 16 and October 18, respectively. The date of the outbreak of red tide dominated by C. polykrikoides in the study area was approximately 50 days later than that off the Kohung areas in 1997 and the surface water temperature when the red tides outbroke in the former area was 6$^{\circ}C$ lower than that fur the latter area. The maximum abundance of C. polykrikoides on September 16, October 7 and 18 were 5, 14, and 463 cells $m\ell$$^{-1}$ , respectively. The growth rate of C. polykrikoides, isolated from the study area, was 0.3~0.4 d$^{-1}$ at 20~$25^{\circ}C$, which enable this species to reach the maximum concentration without being transported from the adjacent waters containing already made red tide patches. The outbreaks of red tides dominated by C. polykrikoides in the study area and off Kohung have occurred when and/or where the concentrations of diatoms were low. This evidence suggests that the outbreak of red tides dominated by C. polykrikoides is adversely affected by the high diatom concentrations or the conditions favorable for the growth of diatoms.