• 한국축산식품학회지
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• 제30권4호
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• pp.568-574
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• 2010

This study introduced a chick embryos’ infection model to elucidate the pathogenesis mechanism of Pseudomonas aeruginosa, which causes serious diseases in human after ingestion of P. aeruginosa-contaminated animal originated foods. The embryonic chick model is able to give a rapid and relatively inexpensive method to assess bacterial pathogenicity compared to embryos of other vertebrates. Embryos were infected with P. aeruginosa and elastase-deficient P. aeruginosa. After infection with P. aeruginosa cells, total bacterial cell numbers and gelatinase activities in the embryos were compared. Thereafter, precartilage condensation and chondrogenesis were assessed by peanut agglutinin (PNA) binding on day 3 and by Alcian blue staining for sulfated proteoglycans on day 5, respectively. P. aeruginosa significantly increased in embryos, resulting in abnormal limb development, whereas P. aeruginosa defective in elastase activity partly impaired proliferation. In addition, P. aeruginosa-infected chick embryos significantly stimulated the production of matrix metalloproteinases. Several analyses showed that elevated proteases suppressed the proliferation and survival of chondrogenic cells. The results show that this infection model was a useful assay to determine the virulence mechanism of P. aeruginosa in human after intake of microbiologically contaminated foods.

• 한국한의학연구원논문집
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• 제2권1호
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• pp.226-235
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• 1996

This experiment was performed in order to study the effects of Gyumsuyukgunjun on the injured tracheal tissue induced $SO_2$ in rats. Healthy adult male rats weighting about 250g were divided into 4 groups - the Normal group, the Control group, the group of Gyumsuyukgunjun administration for 5 days after $SO_2$ gas exposure (Sample I), and the group of Gyurnsuyukgunjun administration for 10 days before and for 5 days after $SO_2$ gas exposure (Sample II). The results were obtained as follows ; 1. In the trachea Control group, the lesion of the ciliated epithelium was severe and the mucus secretion of the respiratory tract was increased significantly. 2. In the trachea of Sample I group, the lesion of the ciliated epithelium and the mucus secretion of the respiratory tract were decreased compared with Control group. 3. In the trachea of Sample II group, the lesion of the ciliated epithelium and the mucus secretion of the respiratory tract were decreased compared with Control and Sample I group. According to the above results, Gyumsuyukgnjun has significant effects on the injuried tracheal tissue caused by $SO_2$ in rats.

• Applied Microscopy
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• 제27권2호
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• pp.155-163
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• 1997

Ultrastructural and histochemical studies were carried out on the prostate gland of a Korean slug (Incilaria fruhstorferi) with light and electron microscopes. The results are as follows. The endothelial tissue of the prostate gland is constituted with tall and narrow ciliated columnar cells, irregularly shaped cells and gland cells in which are included the elongated oval or ellipsoid nucleus those are surrounded by curved membrane. The endothelial tissue of the prostate gland is composed by type-A, B, C and type-D gland cells, and the glanules of type-A, B, and D gland cells are certified to neutral mucopolysaccharide because are related by PAS-alcian blue (pH 2.5) , but the other hand type-C gland cell is only weakly reacted to PAS, but strongly reacted by Million reaction. The glanules of type-A gland cell are small size (about $0.4{\mu}m$) and are seen high electron density, but the glanules of type-B gland cell are large size (about $0.7{\mu}m$) and glanule density is same type-A glanules. Long ellipsoid type-C gland cell contained round nucleus which is well developed beterochromatin in, and that small oval glanules (size, about $0.9{\mu}m$) of moderate high electron density which are formed a group of large glanule together with $4\sim5ea$, but type-D gland cell possessed round small nucleus are seen high electron dense glanules (size, $0.8{\mu}m$).

• 한국패류학회지
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• 제11권2호
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• pp.135-145
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• 1995

A morphological andk histochimical study on the amterior tintacular antenna of Korean sulg, Incilaria fruhstorferi was conducted under the light microscopic observations. The histological sturctures of the antenna were apparently divided into three parts such as the epithelium, the connective tissues and the muscular layers. The cells forming the antenna were classified into several types on the basis of their morphological and histochemical characteristics. The simple columnar epithelium cotering the whole antenna was composed of supporting cells, sensory neurons and type-a clear cells. The connective tissue was consisted of dispersed large cells, type-b clear cells and 7 types of secretory cills such as type-A, type-B, type-F, thpe-G, type-H, type-J and type-K. The large cells found in the form of group situated only in the stalk of the antenna. The large cells possessed relatively small nuclei as compared with their cytoplasm. The cytoplasm positively reacted upon alcian blue, and the nucleus was PASpositive. The type-a and type-b clear cells which were irregular in shape showed no evident reaction against various stains employed in the present study. The secrtory cells were observed mainly in the connective tissues and in the muscular layers. Histochemical components of the type-A, type-B and K were identified as acid mucopolysaccharides and those of type-F and H were neutral mucopolysaccharides. The muscular layders supporting the epithelium possessed the type-B and F secretory cells which were also observed in the connective tissues.

• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1993년도 제27차 학술대회 초록집
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• pp.67-67
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• 1993

기관 및 후두의 점막표면은 중이나 이관과 마찬가지로 점막섬모 정화작용, 면역글로린, 항세균 효소 등의 다양한 점막 방어기전에 의해 보호되고 있는데 기관 및 후두의 분비선과 분비세포의 형태에 관한 보고는 많으나 분비활성도와 연관된 분비조직의 발생에 대한 연구는 충분치 못하다. 저자들은 백서 기관 및 후두의 분비조직의 발생을 알아보고 향후 기관 및 후두의 발생형태학적 연구의 기초자료로 삼기 위해서 임신 16일부터 생후 21사이의 백서를 이용하여 H & E 염색, AB-PAS 염색과 lysozyme의 면역조직화학적 방법을 통하여 백서 기관 및 후두분비조직의 발달을 연구하였다. 그 결과 백서 기관 및 후두의 분비기능은 출생후 폐의 통기와 함께 활성화되는 것으로 해석되었다.

• 대한수의학회지
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• 제60권2호
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• pp.97-100
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• 2020

A 7-year-old Maltese was presented to a veterinary clinic for a history of anorexia, soft feces, and anemia. During abdominal sonography and computed tomography imaging, splenic mass was suspected. Laparotomy revealed the bloody ascites, and the enlarged spleen containing a large spherical mass. Microscopically, the splenic parenchyma was replaced by neoplastic cells with cytoplasmic vacuolation and abundant myxoid matrix. Cellular vacuolation and myxoid matrix were positive to Oil-Red-O and Alcian-blue stains. The neoplastic cells were positive to vimentin on immunohistochemistry. The case was diagnosed as myxoid liposarcoma. Based on a poor prognosis, the dog was euthanized three weeks after splenectomy.

• 한방안이비인후피부과학회지
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• 제14권2호
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• pp.231-241
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• 2001

Mahwang-Shin-Gung-San has been found inhibiting the mast cell-mediated anaphylactic reaction. This report describes an inhibitory effect of Mahwang-Shin-Gung-San (MSGS) on the immediate-type cutaneous allergic reactions. MSGS has concentration -dependently inhibited the ear swelling response induced by compound 48/80 in mouse by intradermal injection. The mast cells in mouse ear tissue undergone ear-swelling response by compound 48/80 were stained by alcian blue/nuclear fast red. MSGS significantly inhibited the compound 48/80-induced degranulation from mast cells in ear tissue. MSGS concentration-dependently inhibited the histamine release from the rat peritoneal mast cells (RPMC) by compound 48/80. We also studied the effect of MSGS on mast cell-dependent passive cutaneous anaphylaxis (PCA) activated by dinitrophenyl IgE antibody. MSGS showed potent inhibition of PCA by oral administration. These results indicate that MSGS inhibits immediate-type allergic reactions by inhibition of mast cell degranulation in vivo and in vitro.

• 한국동물학회지
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• 제30권1호
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• pp.53-70
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• 1987

실험에 사용한 플라나리아는 Dugesia japonica Ichikawa et Kawakatsu로서 핵형분석에 의하여 종을 확인하였다, 이 종의 유조직을 구성하는 세포들의 세포화학적 및 미세구조적 특징을 밝히고자 본 실험을 행하였다. 세포화학적으로 방법으로는 hematoxyline-edsin, periodie, acid-schiff(PAS),PAS-alcian blue 반응 그리고 methylene blue-basic fuchisn 두으로 염색으 시행하였고, 유조직의 미세구조를 투과전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 유조직에는 두 조의 유조직 세포 이외에 봉상체형성세포와 종의 호염기성세포, 1종의 호산성세포 그리고 투명과립세포 7종의 과립세포가 관찰되었다. 1.유리유조직세포(Free parenchymal cell): 핵막 바로 주위에 세포질에는 다수의 chromatoid body가 존재하였고 세포질에 비하여 핵은 매우 컸다. 핵질에서는 뚜렷한 인을 관찰할 수 있었고 큰 염색질은 핵질에 균일하게 많이 분포되었다. 2.고정유세포( Fixed parenchymal cell): 이 세포는 불규칙한 모습으로 많은 세포돌기를 내고 있었고, 세포질에는 전자밀도가 매우 낮은 투명질에 과립성소포체, mitochondria 그리고 Golgi체등이 발달되어 었었다. 3.봉상체 형성세포(Rhabdite-forming cell): 세포질에는 전자밀도가 매우 높은 봉상체과립(0.3 x 0.9 $\mu$m)들을 포함하였으며 과립성소포체,mitochondria 그리고 Golgi체 등의 세포소기관도 매우 발달되어 있었다. 4. A형 호염기성과립세포(Basophilic granule cell type A): 세포질내에는 alcian blue에 강한 양성반응을 나타내고 전자밀도가 매우 높은 불규칙한 형태의 과립 (1.4 x 0.7 $\mu$m)들이 밀접되어 있었다. 5. C형 호기성과립세포(Basophilic granule cell type C): 세포질에는 PAS반응에 양성을 나타내는 작은 과립(0.2$\mu$m)들이 분산되어 있었다. 이 유형의 과립들은 근육층에서도 관찰되었다. 6. D형 호기성과립세포(Basophilic granule cell type D): 생긱기관 주위의 유조직에서만 관찰되었으며 세포질에는 PAS반응에 강한 양성을 보이고 또한 약한 eosinophilic도 나타내는 원형의 과립들이 대부분 집단을 이루웠다. 7. E형 호기성과립세포(Basophilic granule cell type E): 생식기관 주위의 유조직내에서만 관찰되었으며, 세포질내에는 PAS양성과립(약0.2$\mu$m)이 극소수 분산되어 있었다. 8. 호산성 과립세포(Eosinophilic granule cell): 세포질에는 eosinophilia를 나타내며, 전자밀도가 매우 높은 구형 또는 불규칙형과립(0.3$\times$0.2-8$\times$0.4$\mu$m)들이 존재하였으며, cisternae가 넓은 과립성소포체도 매우 발달되어 있었다. 9. 투명과립세포(Transparent granule cell): 본 실험에서 실시된 세포화학적 염색반응에서 음성을 나타내는 투명과립을 포함하고 있는 이같은 세포들은 D형 호염기성과립세포 주위에서 주로 관찰되었다.

• 대한치과교정학회지
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• 제26권6호
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• pp.667-676
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• 1996

We have examined the in vitro stage-related chondrogenic potential of human mandibular and limb bud mesenchyme cells using micromass culture. Our results indicate that limb bud mesenchyme cells as early as stage 16 by Carnegie system (37 days), well before the initiation of in vivo chondrogenesis, have chondrogenic potential which is expressed in micromass culture. These results are correlated with stage-related chondrogenic potential of human limb bud in vivo as a result of Alcian blue staining. The proliferation of chondrogenic cells increased in the first 3 days after culture and then decreased. These results were correlated with the cell cycle analysis of which the number of $G_0^1/G_1$ phase increased markedly after 3 days of culture, while the percentage of cells in S phase was decreased. On the other hand, it was rarely differentiated in the mandible. We examined the effects of two PKC modulators such as phorbol 12-myristate 13-acetate (PMA), a potent activator of PKC, and staurosporine (STSN), an inhibitor of PKC. PMA inhibited the chondrogenesis, whereas STSN promoted the chondrogenesis in a dose dependent manner. In addition, PMA exerted no inhibitory effect when the cells were pretreated for 24 h with STSN, implying that the chondrogenic events might be settled at an early step in vitro and FKC may act as a negative modulator. Collectively, these results demonstrate, for the first time, the stage-related chondrogenic potential of human mandibular and limb bud mesenchyme cells and the role of PKC during chondrogenesis in vitro & in vivo.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제15권1호
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• pp.49-61
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• 1993

The transplantation of cartilage, especially auricular cartilage, has assumed a role of importance in the field of plastic and reconstructive surgery. From long years ago, many reports have appeared in the literature describing the experimental and clinical results of the use of cartilage. At present, the evidence for survival of autograft of cartilage is admitted, But, the results for interrelationship between the bone and cartilage grafts with or without perichondrium is not so conclusive. The purpose of this study were observed as to whether autogenous cartilage grafts were fixed by means of tie with 4-0 vicryl and fibrin adhesive on the femur or microscopic findings of union state in 16 rabbits. We sacrified the experimental animals after 1, 2, 4, 6 weeks postoperatively and made the specimens as a routine laboratory procedures and stained with Hematoxylin-Eosin stain, Verhoeff-van Gieson elastic fiber stain, and alcian blue periodic acid-Schiff(AB-PAS) for mucopolysaccharide. Histologic evaluation was performed under microscope. The obtaind results were as follows : 1. Fibrous union was formed between the grafting cartilage and the femur, nor any findings of calcification and formation of new bone. 2. Partial fibrous adhesion was observed in fibrin adhesive groups on 6 weeks postoperatively. 3. Appositional growth has performed more in fibrin adhesive groups than tie groups. 4. There are little difference in both for new copillary proliferation and fibroblast activations. 5. Degenerative changes have apperared more in tie groups than adhesive groups, but not related to the healing periods.