• 한국동물위생학회지
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• 제26권1호
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• pp.73-80
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• 2003

In order to obtain information on murine model for foot and mouth disease virus(FMDV) type Asia 1, we studied whether guinea pig was a suitable model for studying FMDV. Apparently healthy 3 months old albino guinea pigs and unweaned 3 days old Swiss albino mice were used for this study. Total of 8 guinea pigs were divided into the infected(n=5) and control(n=3) groups. The incubation period of FMDV in the guinea pigs were roughly 2 days and the viremia persisted for 3 days in the guinea pigs. Mice inoculated with the plasma from control guinea pigs did not show any sign of viremia. The plasma were titrated by virus neutralization test using suckling mice as an indicator host. The mean virus neutralizing antibody titers of infected guinea pig at 3 DPI, 4 DPI and 5 DPI were log$\_$10/2.16, log$\_$10/ 3.39 and log$\_$10/ 3.44, respectively whereas there was no neutralizing antibody titer in control group. The difference between the mortality pattern and mean virus neutralizing antibody titer of infected and that of control group at day 3, 4, 5 were statistically significant(p<0.0l).

• 한국자원식물학회지
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• 제14권2호
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• pp.169-174
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• 2001

2000년 5월 중순부터 10월까지 채집하였다. 이 중 2속 4종을 확인하였고 6균주를 분리하여 모두를 자실체 유도실험에 이용하였다. 그 결과 동충하초 [Corcyceps miliraris(CHO-7410)] 1균주, 눈꽃동충하초[isaria japoica (CHO-6713, CHO-7413)] 2균주를 상업적이나 의약품개발에 있어서의 시료등 각 산업에있어서 전반적으로 이용가치가 충분히 있다고 사료되어 우량균주로 선발하였다.

• 대한치과의사협회지
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• 제11권6호
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• pp.383-386
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• 1973

The effects of isoproterenol, a sympathomimetic amine, on the mast cells in the oral mucosa were cytochemically studied in twenty four healthy male albino rats weighing approximately 100gm. seven groups of three each were injected intraperitoneally, as a single dose of 8mg/100gm. body wt., (dissolved in 0.9% saline 0.2㎖). Each groups were sacrificed following ether anesthesia at he time indicated, 1. 2. 3. 5. 7. 10. 14days after only a single injection. One normal group is not administered with any substances. The sections of oral mucosa were stained with 0.005% toluidine blue (Mowry's method) and alcian blue (pH 2.5). We found that isoproterenol made an initial decrease in the metachromasia and alcian blue affinity, and the number of mast cell, followed by an subsequent return to normal.

• 약학회지
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• 제20권1호
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• pp.92-96
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• 1976

A series of experimental groups has been studied in the state of hypolycemia caused by a single intraperitoneal injection of insulin after 24 hrs of fasting albino rats and then the variation of LDH activities and LDH isozyme patterns in the liver, muscle and serum had been reported. The total LDH activity has been elevated in the liver and the muscle above the average level for control group, but increased continusly during 20 min and decreased in the 20-45 min intervals and increased again 45 min in the serum. The change of LDH isozyme patterns had been shown that in the liver LDH$_{5}$ was increased, LDH$_{4}$ was decreased and in the muscle LDH$_{1}$ was diminished by 30 min ws restored again after 45 min and LDH$_{2}$ decreased about 94 percentage at 30 min, decreased by 45 min and increased greater again after 45 min and in the muscle LDH$_{3}$, LDH$_{4}$, and LDH$_{5}$ were increased to the greatest by 20 min, decreased in 20-45 min intervals and increased again after 45 min.

• Restorative Dentistry and Endodontics
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• 제6권1호
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• pp.133-139
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• 1980

In this experiment, etching was performed on the incisor enamel surfaces of 13 Albino rats, weighted about 130gm, with both 30% and 50% concentrations of the phosphoric, citric acids, and EDTA solution for 60 seconds. Immediately after extraction of the animal teeth, laboratory procedures for scanning electron microscopic observations were made and the author derived the following conclusions: 1. On the enamel surface treated with 30% and 50% phosphoric acids, all of the periphery, center of the enamel rods, and the enamel matrices were severely etched. 2. In the etched with 30% citric acid group, only the small part of the enamel rods were etched out. In case of 50% citric acid the periphery, center of enamel rods were partially or entirely etched, and the intermatrix spaces were widened. 3. In the 30% and 50% of the EDTA etched groups, the periphery of the enamel rods explicitely were etched, and the spaces between matrices were widened.

• 한국환경농학회지
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• 제31권3호
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• pp.264-270
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• 2012

본 연구에서는 포공영의 에탄올 추출물의 중금속 중독에 의한 손상 억제 효과를 측정하였다. 현대 사회에서 환경으로부터 오염이 우려되는 납과 수은을 실험동물에 노출시켰고, 납과 수은에 노출된 흰쥐에서 포공영이 이러한 중금속 노출로 영향을 받는 체중변화, 간 및 신장에 미치는 영향을 혈액세포분석 및 혈액 내의 생화학적 분자에 대한 조사하였다. 실험 결과, 포공영을 투여 받은 실험군은 중금속 투여로 인하여 발생하는 체중감소의 개선효과가 나타났고, 혈액세포의 변화에 있어서도 중금속 노출로 감소되었던 RBC의 수를 증가시켰고, 증가된 WBC 및 platelet의 수를 정상수준으로 회복시켰다. 간 및 신장의 손상 지표들로 측정한 Bilirubin, ALP, GOT, GPT, LDH, ceatinine 및 BUN에 있어서도 중금속 노출로 증가된 수치를 농도의존적으로 감소시켰다. 또한 혈액내에서의 중금속 잔존량을 측정한 결과, 포공영 추출물 투여로 인하여 농도의존적으로 그 잔존량이 감소하였다. 따라서, 포공영 에탄올 추출물은 체내 노출된 납과 수은에 대한 독성을 완화시켜주는 것으로 사료된다.

• Journal of Ginseng Research
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• 제30권3호
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• pp.106-111
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• 2006

Adverse changes in individual's biochemistry under heavy metal stress are directly linked with its metabolic activity and health status. The present investigation highlights the differences in protecting role of Panax ginseng extract against mercuric chloride induced alterations in serum proteins. The assessment was based on dividing fifty albino rats into two sets, one for acute and the other for sub-acute study. All the sets had five groups with five albino rats in each i.e. control group, mercuric chloride treated group, Panax ginseng extract treated group, mercuric chloride followed by Panax ginseng extract treated group and Panax ginseng extract followed by mercuric chloride treated group. Mercuric chloride was given orally 0.926 mg/kg body weight for acute set and 0.044 mg/kg body weight for sub-acute set after LD50 (9.26 mg/kg body weight) determination by probitt analysis. 10 mg/kg body weight Panax ginseng extract was given in both acute and sub-acute sets after incorporating safety trials. The control group received tween-20 and distilled water only. The result exhibited significantly reduction (P<0.01) in serum protein, albumin and globulin following mercuric chloride intoxication whereas significant (P<0.01) enhancement in other groups with Panax ginseng extract as an ingredient confirming its protective role. All serum samples were also electrophoresed in 10% SDS with standard marker using discontinuous buffering system. Gradual disappearance of alpha-2 and beta-1 globulin bands from electrophoretic pattern was observed, while a single sharp band was observed between beta-2 and gamma globulin in serum protein pattern of acutely mercuric chloride treated rats. However, this band could not be visualized in sub-acute studies. Panax ginseng extract exhibits a better protection after acute intoxication.

• 대한치과의사협회지
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• 제9권5호
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• pp.235-239
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• 1971

The effects of folic acid on the oral mucosa of albino rats were histochemically studied in the twenty four male rats, weighing abut 100gm. Seven groups of 3 each were injected with 0.2ml folic acid (folic acid 0.5mg. was dissolved in physiological saline 0.2ml) subcutaneously, during 1,2,3,5,7,10 and 14 days respectively. Oral mucosa of rats sere removed from upper molar region and fixed in 10% formalin, cold absolute alcohol, Carnoy's solution and acetone. The serial sections were histochemically stained by McManu's PAS reaction, Mowry's metachromasia, alloxan-Schiff reaction, and azo dye method for alkaline phosphatase. The comparative staining method was hematoxylin-eosin stain. The results were as follows: 1) Alkaline phosphatase reaction of stratum spinosum and stratum granulosum tended to increase after 7 and 10 days of folic acid administration. 2) PAS reactions of basement membrane and lamina propria increased after folic acid administration. 3) Metachromasia of stratum spinosum and stratum granulosum were slightly increased after 3,5 and 7days of folic acid administration and returned to the level of Control after 10 days. 4) In the oral mucosa, alloxan-Schiff reaction increased after 7 days of folic acid administration.

• Journal of Nutrition and Health
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• 제17권2호
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• pp.94-100
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• 1984

The effect of methionine supplementation to the isolated soyprotein(ISP) diet on the growth, body metabolism and composition of the Albino male rats was studied. Three levels(0.3,0.6 and 0.9%) of methionine were supplemented to the ISP diet with the constant levels of energy and protein of 3,600 kcal/kg and 20%, respectively. The body weight and weight gain of the growing rats were significantly increased by 0.3% methionine supplementation to the ISP diet compared to the ISP diet(P< 0.05).The effects of methionine supplementation to the ISP diet tended to be larger with increasing of the level of methionine supplementation, 0.6 and 0.9%, were statistically insignificant. Food and gross energy intake of growing rats fed the ISP diet or the ISP supplemented with methionine diet were lower than those fed the casein diet(P< 0.05). FER and PER of all the methionine supplemented diets were higher than those of the ISP or casein diet (P< 0.05) without significant differences among the supplementation levels of methionine to the diets. The weight gain of adult rats fed 0.9% methionine supplemented ISP diet were higher than those of the other treatments with significant difference. The effects of methionine supplementation to the ISP diet on the protein digestibility, BV, NPU, N-balance, N-retention, and body and liver compositions were not significant.

• Applied Microscopy
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• 제14권2호
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• pp.66-80
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• 1984

The organic phosphorus compounds have been widely used as an insecticide, since toxicity of these compounds is especially drastic to the insects than to men and other mammals. The organic phosphates are rapidly hydrolized and hence have little cumulative and ecologic effects. However, due to their acute toxic effects organophosphate have recorded rather high fatalities in men and domestic animals. The organic phosphorus compounds are powerful inhibitors to the carboxylic esterase enzymes such as acetylcholinesterase and pseudocholinesterase. As a result of firm binding characteristics of phosphate radicals to the active sites of enzyme, the activities of these enzymes are inhibited by the organophosphates. The organophosphates such as diazinon is easily observed from skin, gastrointestinal tract, conjunctivas and respiratory tract, and it is converted to more toxic form during metabolism in the liver The present study was carried out in order to investigate the hepatotoxicity of diazinon by observing the changes in the ultrastructure of cytoplasmic organelles of hepatic cells in albino mice. The animals were killed at 6, 12 and 24 hours after administration of 25mg/kg diazinon. The piece of hepatic tissue obtained from each animal was ultrathinly sectioned. The specimens stained by uranyl acetate and lead citrate double contrast methods were observed with JEM model 100B electron microscope. The results obtained were as follows: 1) A prominent dilatation and sacculation of the cisternae of rough endoplasmic reticulum associated with detachment of membrane bound-ribosomes, and disaggregation of the free ribosomes were recognized. 2) The hypertrophy of the smooth endoplasmic reticulum associated with depletion of the glycogen particles was observed. 3) The atrophy of cisternae of Golgi complex was observed. 4) A large number of secondary lysosomes (autophagic vacuoles and residual bodies) were formed. Consequently it is suggested that diazinon would induce disorganization of the cytoplasmic organelles of hepatocytes in albino mice.