• Biomolecules & Therapeutics
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• 제3권3호
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• pp.223-228
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• 1995

In an attempt to define the effects of biphenyldimethyl dicarboxylate (DDB) on the lipid peroxidation and oxygen free radical scavenging enzymes activities in mercuric chloride-induced hepatotoxic rats, we studied malondialdehyde (MDA) level and the activities of catalase and superoxide dismutase (SOD) in liver of the rats at 24 hr after the injection of mercuric chloride. Sprague-Dalwey albino rats were injected subcutaneously with mercuric chloride (5 mg/kg) only and mercuric chloride (5 mg/kg) plus. DDB (200 mg/kg/day, p.o) is administered for 4 days prior to 3 days from the injection of mercuric chloride. The group treated with mercuric chloride showed significantly higher MDA level and lower catalase and SOD activities as compared with that of control group. The group treated with mercuric chloride plus DDB showed significantly lower MDA level and catalase activity and higher SOD activity as compared with that of mercuric chloride-treated group. These results suggest that the excessive oxygen free radicals resulting from the depression of superoxide dismutase activity is an important determinant in the pathogenesis of mercuric chloride-induced hepatotoxicity and DDB has antioxidant effects.

• 한국식품영양학회지
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• 제5권2호
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• pp.90-98
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• 1992

This study was divised to observe the effects of defatted(by petroleum ether) Korean ginseng on the cholesterol level of Sprague-Dowley Albino male rats by alternating the diet. 130 heads of weaned rats having 83$\pm$4g of body weight were fed with each stock diets supplemented with 0.5, 1.0 and 3.0% defatted ginseng powder for 4 or 8 weeks, and after that fed with stock diet only for 8 or 4 weeks. Total cholesterol and free form cholesterol contents in serum, liver and aorta of them were determined, and compared with the each corresponding group which was fed with defatted ginseng control diet for 12 weeks. Total cholesterol and free form cholesterol contents in the serum were increased caused by the alternating diets which was fed on 0.5, 1.0 and 3.0% defatted ginseng diet for 4 and 8 weeks after being fed on stock diet for 8 and 4 weeks, conversely total cholesterol contents in the aorta were decreased by the same alternating diets. Free form cholesterol contents in the serum, liver and aorta of the group which was fed on 1.0% defatted ginseng diet 8 weeks after being fed on stock diet for 4 weeks were increased, but that of the group which was fed alternately on 3.0% defatted ginseng diet were decreased than the corresponding defatted ginseng control diet group.

• Parasites, Hosts and Diseases
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• 제22권2호
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• pp.181-189
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• 1984

우리나라산(전남 강진 및 경남 금해시)미꾸리에서 이전고무극구흡충(Echinostoma cinetorchis)의 피낭유충을 처음으로 검출하고 횐쥐에 감염시킨 후 성충을 얻어 종을 확인하였다. 또, 미꾸리의 피낭유충 감염상황과 횐쥐에서의 간단한 발육과정을 관찰하였다. 결과를 요약하면 다음과 같다. 1. 미꾸리 46마리중 5마리 (10.9%)에서 담낭유충이 검출되었고 감염된 미꾸리당 감염대은 평균 34.4개이었다. 피낭유충은 미꾸리의 후복벽에서만 발견되었다. 2. 실험감염 횐쥐에서 감염 6∼28일까지 총 충체회수률은 24.6%이었으며 감염기간이 길어짐에 따라 회수률은 감소되었다. 3. 충체는 두증과 두련(37∼38개)이 뚜렷하고 고환이 이전하거나 소실되는 점으로 보아 E. sinetorchis Ando et Ozaki, 1923로 동정되었다. 4. 충체의 발육은 감염6일부터 10일 사이에 가장현저하였으며 이 기간중 특히 생식기관의 발육이 비생식기관에 비해 빠르고 현저하였다. 이상의 결과로 볼 때 우리나라에서 미꾸리가 이전고구극구흡충의 제이중간숙주 역할을 하고 있음이 입증되었고 실험적으로 얻은 성충의 형태에서 고구의 이전 또는 소실이 종의 특성임이 재확인되었다.

• 한국동물학회지
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• 제12권3호
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• pp.94-102
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• 1969

盛熱한 albino 雄性 흰쥐를 對照群과 實驗群으로 나누어 對照群에는 0.9% 生理食鹽水를, 實驗群에는 methylene blue(38mg/kg, 食鹽水에 溶解 pH 7.4)를 各各 腹腔注射處理하였다. 約 30 分後 兩群 共히 總線量 360 rads 인 $^60 Co$의 r 線을 一時 全身照射하였다. 照射後 212時間에 걸쳐 血淸總乳酸脫水素酵素의 含量(Cabaud and Droblewski 1958)과 血淸을 비롯하여 肝, 心臟, 精巢組織의 乳酸脫水素酵素의 同位酵素vnfortys(Preston et al., 1965)을 各各 測定하여 methylene blue 의 放射線에 對한 防禦　果를 硏究調査하였다. 一般的으로 血淸總乳酸脫水素酵素의 含量은 兩群共히 r 線照射後 初期에 增加하나 特히 methylene blue 實驗群인 處理群은 對照群인 生理食鹽水群에 比하여 15 및 64 時間區에서 增加의 遲滯性을 나타내었다. 血淸과 肝, 心臟 및 精巢組織의 乳酸脫水素酵素의 同位酵素는 兩群共히 線照射後 40과 116 時間區에서 陽極쪽의 同位酵素 푸랙숀 含量은 減少한다. 照射群 methylene blue 處理群의 血淸과 肝 및 精巢組織 乳酸脫水素酵素의 同位酵素패턴에서는 對照群과 別差異가 없었으나 特히 methylene blue 處理群의 心臟組織은 對照群에 比하여 若干의 增減의 遲滯性을 나타내었다. 兩群共히 照射後 血淸乳酸脫水素酵素含量의 增加는 同位酵素푸랙숀 1의 增加에 基因된다고 思料되며 이상의 結果로 미루어 보아 總乳酸脫水素酵素 活性과 心臟組織의 乳酸脫水素酵素의 同位酵素패턴에 있어서 methylene blue가 放射線防禦　果가 있는 것으로 思料된다.

• Parasites, Hosts and Diseases
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• 제24권1호
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• pp.63-70
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• 1986

The tegumental ultrastructures oi Echinostoma hortense adults were observed by scanning electron microscopy. The worms of 4 weeks of age were harvested from albino rats experimentally infected with the metacercariae obtained from the loach. The results were as follows: 1. The worms were leaf-like and their anterior end portion, including oral sucker and head crown, ventrally curved to face posteriorly. The tegument of whole body was wrinkled transversely and covered with cobblestone-like cytoplasmic processes. 2. The oral sucker had roundly swollen (type I ) sensory papillae on the ventral half of its lip and mini-ciliated knob-like (type I ) sensory papillae, arranged in 2∼3 rows, on the dorsal outer surface. 3. Aspinous ventral sucker had many of type I papillae arranged in a circular band on its outer surface. The tegument around the genital opening was of similar feature to the ventral sucker, but sensory papillae were hardly found around the former. 4. Scale-like spines with broad base and round tip were distributed densely on the tegument anterior to the ventral sucker but they became sparse in posterior half of the ventral surface, finally to disappear at posterior extremity. A few number of type I papillae were observed on the ventral surface. The results suggest that the tegument of 5. hortense is similar to that of other echinostomes etapecially 5. revolutum. But the number and arrangement of collar spines, and/or the type and distribution of sensory papillae seem characteristic features of E. hortense differed from other echinostomes.

• Biomolecules & Therapeutics
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• 제6권1호
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• pp.9-13
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• 1998

ln an attempt to define the effects of hyperbaric oxygen treatment on the lipid peroxidation and oxygen See radical reactions in rats exposed to carbon monoxide, we studied malondialdehyde(MDA) level and activities of catalase and superoxide dismutase in the heart of the rats exposed to carbon monoxide. Male Sprague-Dawley albino rats weighing 240 to 260gm were used. Experimental groups consist of Control group (=breathing with air), HBO group(=exposed to hyperbaric oxygen(HBO, 3ATA, 100%) after air breath), CO group(=exposed to CO(3,970 ppm) after air breath), CO-Air group(=exposed to CO after air breath followed by air breath) and CO-HBO group(=exposed to CO after ai. breath followed HBO treatment). The CO group showed significantly higher MDA level, catalase activity and SOD activity as compared to that of control group. The CO-HBO group showed significantly lower MDA level as compared to that of CO group, and did not show significantly lower catalase activity and SOD activity as compared to that of CO group. These results suggest that the excessive oxygen free radicals is an important determinant in pathogenesis of Co-induced cardiotoxicity and HBO inhibits the lipid peroxidation caused by excessive oxygen free radicals in the heart of the rats exposed to carbon monoxide.

• 한국식품과학회지
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• 제10권2호
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• pp.105-108
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• 1978

This experiment was designed to observe some influence on the serum components in rats by feeding basal diet (Protein contents: 20.0%; lipid contents: 3.5%) with soft water (Water, Carbonated cider, Cola and Fanta). Fourty male Albino rats were used as the experimental animals and the subjects were devided into four feeding group and each group was fed on the corresponding diet for six weeks. The contents of protein, lipid and cholesterol were determined in their serum. The obtained results are summarized as follows. 1) The protein contents in the serum was decreased by the feeding of the Carbonated cider, Cola and Fanta compared with Water experimental group. 2) The lipid contents in the serum was similar to each feeding experimental group. 3) The total cholesterol and ester form cholesterol in the serum were decreased by the feeding of the Carbonated cider compared with Water, but were similar to the feeding of the Cola and Fanta.

• Mycobiology
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• 제33권3호
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• pp.125-130
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• 2005

Pigmentation of ascospore-derived isolates from seven different natural specimens of Cordyceps militaris EFCC C-5888, EFCC C-7159, EFCC C-7833, EFCC C-7991, EFCC C-8021, EFCC C-8023 and EFCC C-8179 was observed on the plates of Sabouraud Dextrose agar plus Yeast Extract at $25^{\circ}C$ under continuous illumination (500 lux). Pigmentation of the wild-type isolates of C. militaris was diverse ranging from yellowish white to orange, while white color was believed as a mutant. Inheritance of pigmentation was found to be controlled by both parental isolates when F1 progeny were analyzed. Pigmentation and mating type were shown to be either independent or distantly linked each other due to the high percentage of non-parental phenotypes among F1 progeny. Crosses between white mutant isolates of C. militaris yielded progeny with wild type pigmentations, indicating that the albino mutations in the parents were unlinked to each other.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제17권4호
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• pp.317-330
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• 1995

The purpose of this study is to observe the stimulant effect of the isolated nerve segment on the peripheral nerve regeneration using silicone tube in rats. Sprague-Dawley female albino rats were used as experimental animals and nerve defects were made by resection of 14 mm-long segment of right sciatic nerves. In control group, the defects were bridged with the silicone tubes. In experimental group, the defects were bridged with the same manner and additionally, 2mm-long nerve segments were inserted at the center of the silicone tubes. 3 months later, the regenerated nerve tissue within the silicone tubes were examined by histologic study. Axonal diameters and numbers of axons were measured and all data were analyzed by using SAS package program. The results obtained were as follows : 1. The experimental group was increased than the control group in general diameters of the regenerated nerve fiber.(P<0.05) 2. The diameters of axons were increased in the experimental group compare to the control group.(P<0.05) 3. The numbers of axons were increased in the experimental group compare to the control group.(P<0.05)

• BMB Reports
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• 제40권3호
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• pp.295-301
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• 2007

Swiss Albino (Rat rattus norvegicus) rats were intraperitoneally injected with a 100 mg $kg^{-1}$ dosage of benzene, a toxic and carcinogenic agent widely used for industrial purposes. Changes in the adenosine deaminase (ADA) activity in the liver, kidney and serum of rats were investigated at 0, 2, 4, 8, 16, 32 and 64 h following injection. Serum physiological was administered to each control group. Enzyme activities were measured spectrophotometrically. Our purpose was to further investigations of some diseases caused by benzene, and present evidence of variations in the activity of ADA enzyme effected by benzene. While benzene caused significant inhibitions in ADA activity in the liver at 16 and 32 h and at 0.05 probability level, no significant inhibition or activation occurred at other test periods (hours). ADA activity did not present any significant variation in the kidneys. It was observed that ADA activity displayed similar patterns in the control groups. Comparisons of ADA activities in the two groups showed a statistically significant decrease between $4^{th}$ and $64^{th}$ hours (p< 0.05), demonstrating a direct correlation between benzene and its effects on ADA enzymes.