• 한국조리학회지
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• 제22권8호
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• pp.149-156
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• 2016

본 연구의 목적은 조리과정에서 발생한 총휘발성 유기화합물질, 미세먼지, 악취, 총부유세균 등의 오염물질들이 조리실내로 분산되어 실내공간이나 조리업 종사자에게는 나쁜 냄새나 작업환경 및 건강피해를 감소하고자 하는 것이다. 이를 위해 기존 공기청정기의 단점을 보완하고, 반영구적이며, 각종 오염물질을 효율적으로 제거하기 위하여 활성탄과 황토를 결합한 바이오세라믹 필터를 bar type으로 제작한 실내용 소형 공기청정기 개발에 중점을 두었다. 측정항목은 총휘발성 유기화합물, 미세먼지, 복합악취, 총부유세균 등 모두 4가지 항목으로 개발된 공기청정기 가동 전과 일정시간 가동 후를 비교하여 측정하였다. 측정결과, 총휘발성 유기화합물의 농도는 평균 $2,500{\mu}g/m^3$에서 $223{\mu}g/m^3$으로 약 91.02%의 높은 제거효율을 보였으며, 미세먼지의 제거율은 평균 농도가 $26.68{\mu}g/m^3$로서 97.51%의 우수한 제거효과가 나타났으며, 복합악취의 희석배수를 측정한 결과 평균 144로 평균 95.20% 감소하였다. 또 총부유세균은 초기 농도가 $787{\sim}814CFU/m^3$에서 $47{\sim}40CFU/m^3$로 약 94% 이상 제거되는 것을 확인하였다. 결론적으로 개발되어진 공기청정기의 바이오세라믹 필터는 오염물질을 제거하는데 우수한 제거효과를 나타냈다. 따라서 공기청정기를 조리실내에서 환기장치와 렌지후드를 병행하여 사용하면 기존의 열악한 조리실 내의 환경이 크게 개선되어질 것으로 사료된다.

• 미생물학회지
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• 제39권4호
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• pp.253-259
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• 2003

미생물학적 실내공기질을 평가하기 위하여, 울산에 위치한 3곳의 유치원을 대상으로, 2002년 4월에서 2003년 1월에 걸쳐 계절별로 공기 중의 미생물 농도를 결정하였다. 미생물의 포집에는 충돌식 공기 채취기를 사용하였으며, 세균수는 Staphylococcus medium과 plate count agar, 진균수는 dichloran rose bengal chloramphenicol agar를 사용하여 측정하였다. Staphylococcus medium에서 생장한 세균의 평균 농도는 827.0 MPN/㎥ 로 83.5~4,149.1 MPN/㎥의 범위였으며, plate count agar의 경우는 평균이 580.3 MPN/㎥로 50.0~2,636.0 MPN/㎥의 범위였다. 계절별 평균값은 여름이 가장 높았고, 가을, 봄, 겨울의 순이었으며, 세균의 집락수와 실내온도는 양의 상관관계가 있었다. 계절에 따라 그람양성의 구균은 전체 세균 집락의 45.6~61.0%, 그람음성의 막대균이 8.5~20.6%를 차지하였으며, Micrococcus spp.가 가장 우점하였다. 진균의 농도는 평균이 660.8 MPN/㎥로 0~1,887.5 MPN/㎥의 범위였으며, 계절별 평균값은 여름이 가장 높았고, 겨울이 가장 낮았다. 실내외 공기의 진균 집락으로부터 Penicillium spp.와 Aspergillus spp.를 확인할 수 있었다. 연구의 결과는 계절에 따른 미생물 농도의 변이와 학교의 실내환경에서 bioaerosol의 허용수준을 결정함에 있어 이를 고려해야 할 필요가 있음을 보여주었다.

• 한국미생물·생명공학회지
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• 제50권2호
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• pp.165-192
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• 2022

본 논문에서는 실외 대기 환경의 바이오에어로졸 혹은 입자상물질의 미생물 메타게놈 특성과 이에 영향을 미치는 기후 및 환경 인자의 영향을 고찰하였다. 시료 채취 지역 및 환경 조건 특성별 대기 중 세균과 곰팡이 농도를 요약 하고, 에어로졸과 PM 시료의 세균과 곰팡이의 메타게놈 특성을 조사하기 위한 비배양법 기반 분석방법과 메타게놈 특성을 정리하였다. 또한, 세균과 곰팡이의 메타게놈 특성과 다양성 및 특성에 미치는 기상 인자와 환경 인자의 영향을 고찰하였다. 대기 중 미생물의 생존, 생장과 분산은 지역 기상 조건 및 대기 오염 물질에 의해 크게 영향을 받았다. 일반적으로 기온이 상승함에 따라 AM 농도는 증가하지만, 여름에는 고온과 강한 자외선의 영향으로 AM 농도가 감소하였다. 습도와 미생물 농도는 양의 상관성을 보이나, 습도가 너무 높으면 AM의 분산이 지연되었다. 이러한 종합적인 고찰 결과는 대기권에서 미생물의 역할과 기능을 이해하고, 이들 미생물에 의해 야기되는 환경 및 공중보건 문제를 해결하기 위한 전략 수립 및 저감 기술 개발에 활용될 수 있다.

• 한국산업보건학회지
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• 제23권2호
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• pp.84-94
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• 2013

Objectives: The objective of this study is to identify the composition of dominant microorganisms in waste handling industries. Methods: We collected airborne bacteria and fungi by agar plate impaction method in recyclable waste sorting industry, food recycling industry, landfill and incineration. Isolated dominant microorganisms were identified by VITEK system or morphological analysis. Results: We isolated totally 330 microorganisms in the process and outdoor. Bacillus was the most dominant genus in the all industries, and Sphingomonas, Acinetobacter, Staphylococcus, and Proteus was dominant bacterial genus. The dominant genus of fungi was Penicillium, Aspergillus, and Cladosporium in each industries. Enterobacter, Pseudomonas, Klebsiella, and Proteus was identified as the dominant gram negative bacteria. The ratio of bacteria being biosafety levels(class 1 or 2) was 58.3~77.8%. Conclusions: This study has investigated the dominant microorganisms in the waste handling industries. The genus of dominant microorganisms was similar among the industries but the composition was different. We used biosafety levels as qualitative method, but further studies are needed about specific process of qualitative evaluation methods.

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 2001년도 추계학술대회 논문집
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• pp.381-382
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• 2001

Bioaerosol은 인공적으로 생성되거나, 혹은 자연적으로 발생하는 생물학적 기원(biological origin)을 가진 물질로 정의한다. 실내공기 중에서 이러한 Bioaerosol 발생의 중요한 원인으로는 실외에서 유입되는 경우와 건물의 HVAC 시스템에 의한 경우, 실내에 거주하고 있는 사람이나 동물에 의한 경우가 대표적이다. 특히 이 세 가지 오염원 중에서 실내 거주자와 동물이 가장 중요한 역할을 하는 것으로 보고되고 있다(Pastuszka 등, 2000). (중략)

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2005년도 국제학술대회
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• pp.254-258
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• 2005

The purpose of this study is to characterize the indoor-outdoor relationship of airborne pollutants and recognize probable sources in inside and outside individual apartments in Seoul metropolitan. Simultaneous air monitoring in inside and outside of the 16 Korean Apartments classified into 2groups: less than 1 year old and more than 4 years old from October, 2004 to February, 2005were sampled f3r airborne pollutants(volatile organic compounds, formaldehyde, respiratory particles, carbon dioxide and bacteria) using the Korean Indoor Air Quality Official Method. The concentrations of $CO_2$, TVOCs, HCHO, bacteria and PM10 in the less than 1 year old apartments were determined to be $773.6{\pm}422.3ppm$, $4,393.8{\pm}2,758.2{\mu}g/m^3$, $98.0{\pm}56.4{\mu}g/m^3$, $254.0{\pm}186.3CFU/m^3$ and $31.7{\pm}14.8{\mu}g/m^3$, respectively, Also, the concentrations of those in the more than 4 years old apartments were determined to be $798.9{\pm}266.5ppm$, $792.7{\pm}398.3{\mu}g/m^3$, $70.0{\pm}30.7{\mu}g/m^3$, $245.6{\pm}122.0CFU/m^3$, $49.7f28.7{\pm}g/m^3$, respectively. The average ratios of the indoor and outdoor concentrations of $CO_2$, TVOCs, HCHO, bacteria and PM10 were 2.2, 3.6, 3.1, 3.9 and 1.4, respectively. These results of this analysis is suggested that $CO_2$, TVOCs, HCHO, bacteria and PM10 in indoor air are both emitted from source within the apartment environment and partly come from outdoor air. With the above considerations in mind, it is suggested that the research for source contribution of indoor air pollutants should be expanded and the detailed interpretation of the results on these needed further study(using principal component analysis(PCA).

• 한국유기농업학회지
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• 제5권1호
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• pp.87-99
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• 1996

Cultural conditions for mass production of the antagonistic bacteria, Bacillus subtills CAP134 against pathogens causing major airborne diseases to apple tree, effect of temperature, pH, carbon and nitrogen source in the culture broth were investigated. The bacterial growth was most vigorous when the temperature and pH of the culture broth was 30~$35^{\circ}$C, and 7, respectively. As for carbon source, dextrose was best followed in order by dextrose(monosaccharides)>sucrose(disaccharides)$\geq$saccharose(di-saccharides)>starch (polysaccharides). Among different sugars, bacterial growth was favored by in the order of brown, black and white sugars, indicating that the bacterial growth might be promoted by the minor elements presented as impurities in the less purified sugars. As for nitrogen source, organic forms were better to bacterial growth than inorganic forms, that is polypeptone was best followed in order by soy sauce, soybeen milk and inoganic nitrogens. Differences in bacterial growth among different forms of inorganic nitrogen were negligible.

• 설비공학논문집
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• 제19권4호
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• pp.291-298
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• 2007

The purpose of this study is to evaluate the efficacy of multizone simulation that enables to grasp of details about microbial contaminant problem in an multistoried apartment. We used actual indoor test data to figure up microbial contaminant level as initial value for the multizone simulation and estimated the various effects of indoor occupant infected with germs such as bacteria and fungus and the performance of air sterilization by using multizone simulation in substitute for infeasible experimental approach. The results show that natural ventilation make ourselves generally useful for removing indoor microbial contaminants. The results also show that the performance of air sterilization reach the maximum in the case of using mechanical ventilation and UVGI air sterilizer. The conclusion is that this multizone simulation is useful tool for actual design method for immune building systems.

• 한국유기농업학회지
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• 제6권1호
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• pp.85-98
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• 1997

Cultural conditions for mass production of the antagonistic bacteria, Bacillus subtills CAP141 against pathogens causing major airborne diseases to apple trees, effects of temperature, pH, carbon and nitrogen source in the culture broth were investigated. The bacterial growth was most vigorous when the temperature and pH of the culture broth was 30~35$^{\circ}$C and 7, respectively. As for carbon source, dextrose was best followed in order by dextrose(monosaccharide) > sucrose(disaccharide) $\geq$ saccharose (market disaccharides) > starch (polysaccharieds). Among different sugars, bacterial growth was favored by in the order of brown, black and white supars, indicating that the bacterial growth might be promoted by the minor elements presented as impurities in the less purified sugars. As for nitrogen source, organic forms were better to bacterial growth than inorganic forms, that is polypeptone was best followed in order by soy sauce, soybeen milk and inorganic nitrogens. Differences in bacterial growth among different forms of inorganic nitrogen were negligible.

• Safety and Health at Work
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• 제6권1호
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• pp.62-70
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• 2015

Background: Animal skin provides an ideal medium for the propagation of microorganisms and it is used like raw material in the tannery and footware industry. The aim of this study was to evaluate and identify the microbial load in oropharyngeal mucosa of tannery employees. Methods: The health risk was estimated based on the identification of microorganisms found in the oropharyngeal mucosa samples. The study was conducted in a tanners group and a control group. Samples were taken from oropharyngeal mucosa and inoculated on plates with selective medium. In the samples, bacteria were identified by 16S ribosomal DNA analysis and the yeasts through a presumptive method. In addition, the sensitivity of these microorganisms to antibiotics/antifungals was evaluated. Results: The identified bacteria belonged to the families Enterobacteriaceae, Pseudomonadaceae, Neisseriaceae, Alcaligenaceae, Moraxellaceae, and Xanthomonadaceae, of which some species are considered as pathogenic or opportunistic microorganisms; these bacteria were not present in the control group. Forty-two percent of bacteria identified in the tanners group are correlated with respiratory diseases. Yeasts were also identified, including the following species: Candida glabrata, Candida tropicalis, Candida albicans, and Candida krusei. Regarding the sensitivity test of bacteria identified in the tanners group, 90% showed sensitivity to piperacillin/tazobactam, 87% showed sensitivity to ticarcillin/clavulanic acid, 74% showed sensitivity to ampicillin/sulbactam, and 58% showed sensitivity to amoxicillin/clavulanic acid. Conclusion: Several of the bacteria and yeast identified in the oropharyngeal mucosa of tanners have been correlated with infections in humans and have already been reported as airborne microorganisms in this working environment, representing a health risk for workers.