• Journal of Life Science
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• v.29 no.2
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• pp.272-278
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• 2019

The silkworm performs sexual reproduction for the production of its healthy offsprings from generations to generations. Parthenogenesis in the silkworm, Bombyx mori acquires immense use in the development of outstanding homozygouse lines with higher viability, hybrid vigour, combining ability and less phenotypic variability, and it can serve as a powerful tool in controlling sex of the offsprings as well as a useful tool in selection of breeding schemes. However, naturally occuring parthenogenesis in silkworm could not be found so far. Fortunately, artificial induction of parthenogenesis is possible in silkworm. So, it is very important to find out novel methods for induction of parthenogenesis. We investigated to attempt to get a novel parthenogenetic method. Accordingly, parthenogenetic studies on between unfertilized in vivo ovarian eggs of live silkworm moth(novel) and unfertilized in vitro ovarian eggs(conventional) taken out from live silkworm moth were investigated by hot water ($46^{\circ}C$), hot air ($46^{\circ}C$) and low temperature ($0^{\circ}C$ and $-20^{\circ}C$) treatments. The best ratio of parthenogenetic eggs was obtained with in vivo ovarian eggs of live silkworm moth rather than with in vitro ovarian eggs taken out from live silkworm moth in all the treatments. The optimum exposure time absolutely depended upon the temperatures of treatments and the forms of in vivo or in vitro ovarian eggs. From these results, we expect that in vivo artificial parthenogenetic treatments on live silkworm moth will be useful for the higher induction of parthenogenesis in the silkworm, B. mori.

• Food Engineering Progress
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• v.21 no.4
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• pp.326-331
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• 2017

A cultivar (Malus domestica cv. Fuji) of apple was selected to make apple peel (AP) powder by three different powdering methods. Frozen AP was thawed and subsequently was dried or ground without drying. After AP was dried by hot-air drying at $60^{\circ}C$ or freeze-drying, the dried AP was ground using a conventional blender. Separately, the thawed AP was powered by using a cryogenic micro grinding technology (CMGT). The ground AP and three types of AP powder were extracted using deionized water, 20, 40, 60, 80, or 100% methanol, followed by vacuum evaporation. The total phenolics contents (TPC), total flavonoids contents (TFC), DPPH, and ABTS radical scavenging capacities of each extract were compared to determine an efficient powdering method. Lyophilized AP powder extract using 60% methanol showed the highest TPC and DPPH radical scavenging capacity. In contrast, 60% methanol extract of the powder by CMGT, resulting in the smallest particle, exhibited the highest TFC and ABTS radical scavenging capacity. This study suggests that the extraction yield of bioactive compounds from AP may be varied according to different powdering methods and that a new powdering process such as CMGT may be applicable to develop functional foods efficiently.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.1
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• pp.59-66
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• 2021

This study aimed to a basic research for the development of dye-sensitized solar cells that the wavelength band required for crop growth is passed, and the wavelength band that is not necessary for crop growth can be used for the generation of electricity. The transmissivity according to the illuminance was about 10% higher in the Blue filter and the Green filter than in the Red filter, but the transmissivity according to the PPFD was about 10% higher in the Red filter and the Blue filter than in the Green filter. In addition, the greenhouse attached with 30% infrared blocking filter was predicted to have a lower air temperature than other greenhouses, but it was investigated that there was no significant difference. Therefore, it was investigated that the application of the infrared cut filter would not be appropriate in a greenhouse that controls the temperature by opening a window. As a result of investigating, it was found that the Green and Blue filter greenhouses had the severe overgrowth and the stems grew weaker. The fresh weight of paprika in the infrared blocking filter greenhouse was the highest at 678.9g, and the growth of Red filter and the control greenhouses was relatively poor. Photosynthetic rate, amount of transpiration, and stomatal conductivity were the infrared blocking filter and control greenhouse higher than others. On the other hand, the water use efficiency did not show a big difference.

• Journal of Korean Tunnelling and Underground Space Association
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• v.24 no.2
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• pp.153-170
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• 2022

Waterjet has been comprehensively used in urban areas owing to a suitable technique for cutting concrete and rock, and low noise and vibration. Recently, the abrasive waterjet technique has been adopted and applied by the Korea Atomic Energy Research Institute to demolish concrete plugging without disturbing and damaging In-situ Demonstration of Engineered Barrier System in the disposal research tunnel. In this study, the use of abrasive waterjet in the tunnel was evaluated for practical applicability and the existing cutting model was compared with the experimental results. As a variable for waterjet cutting, multi-cutting, water flow rate, abrasive flow rate, and standoff distance were selected for the diversity of analysis. As regarding the practical application, the waterjet facilitated path selection for cutting the concrete plugging and prevented additional disturbances in the periphery. The pump's noise at idling was 64.9 dB which is satisfied with the noise regulatory standard, but it exceeded the standard at ejection to air and target concrete because the experiment was performed in the tunnel space. The experimental result showed that the error between the predicted and measured cutting volume was 12~13% for the first cut and 16% for second cut. The standoff distance had a significant influence on the cutting depth and width, and the error tended to decrease with decrement of standoff distance.

• The Korean Journal of Mycology
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• v.25 no.4 s.83
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• pp.257-267
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• 1997

Fruit bodies similar to the Phellinus sp. residing on the mulberry were collected at Yang-yang in Kang-won-do province and one strain of Phellinus sp. was isolated from the fruit bodies. For mass production of the isolated mycelia in a submerged culture, the culture conditions, medium composition, and the effect of various culture systems on the mycelial growth, were investigated. The morphological characteristics of the fruit body were as follows: covered with blackish to black and rough, lower surface with yellowish-brown to dull-brown and smooth, 5-7 cm thick and hard woody. Also, the pure cultured mycelia showed yellowish-brown color, capability of purplish-brown pigment production on the PDA plate media, no-formation of clamp-connection, much binding branch, and enzyme activities such as laccase, tyrosinase and peroxidase. Therefore, pure cultured strain was identified to be Phellinus sp. In the flask culture, the optimum culture conditions for the mycelial production were obtained after cultivation of 8 days at inoculum level of 5%(v/v), media volume of 70 mL, 150 rpm, initial pH 6, and temperature of $30^{\circ}C$. Optimum medium composition from the response surface analysis were determined to be glucose 12.12 g/L, sucrose 12.12 g/L, yeast extract 11.15 g/L, malt extract 11.15 g/L, $KH_2PO_4$ 0.855 g/L and $CaCl_2$ 0.855 g/L. The production of the mycelia after 4 and 8 days of cultivation was 1.95 and 9.89 g/L, respectively. The maximum specific growth rate and productivity were $0.020\;hr^{-1}$ and 1.25 g/L/day, respectively. Among the three different culture systems for the growth of mycelia, the maximum mycelial dry weight of 7.5 g/L was obtained after cultivation of 4 days in the air-lift fermentor under aeration rate of 2.5 vvm. The maximum specific growth rate and productivity were $0.033\;hr^{-1}$ and 1.9 g/L/day, respectively, which were about 1.7 and 4.2 times higher than those of flask culture.

• Korean Journal of Weed Science
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• v.16 no.1
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• pp.14-20
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• 1996

Growth, nutrient uptake and photosynthesis as affected by submersion of shoot in pickerel weed (Monochoria vaginalis Presl.) were determined. The shoots of pickerel weeds in hydroponic culture were subjected to the submerged or emerged condition at 3- or 5-leaf stage for 8 or 10 days. Under submerged condition, growth in plant height was enhanced, but leaf number, leaf area, fresh and dry weight were reduced compared to those under the emerged condition. Similar responses in growth to submergence were obtained with the pickerel weeds rooted in the soil. Under submergence, chlorophyll content increased during the first 2 days, but thereafter remarkably decreased at 3-leaf stage and after the first 4 days at 5-leaf stage. Compared to the emerged condition, uptakes of $NH_4\;^+$-N, $NO_3\;^-$-N, $P_2O_5$ and $K^+$ were reduced, but uptakes of $Ca^{++}$ and $Mg^{++}$ increased under the submerged condition. Photosynthetic rate of shoot under water, measured by $CO_2$electrode, showed the maximum by 210 ${\mu}$moles $HCO_3\;^-$/g F.W. at the 8th day after submergence(DAS) at 3-leaf stage and 320 ${\mu}$moles $HCO_3\;^-$/g F. W. at 6 DAS at 5-leaf stage. These results indicate that pickerel weeds grow much better when the shoot is air-exposed and are less tolerable to submergence at 3 leaf-stage than at 5-leaf stage.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.2
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• pp.140-145
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• 2002

The performances of a parallel current air driven type foam separator were evaluated in the pilot-scale recirculating aquaculture system. The system was stocked with Nile tilapia (Oreochromis niloticus) at an initial rearing densities of $2\%$, $5\%$ and $7\%$ of water volume of rearing tank and reared for 15, 30, 35 days, respectively. The condensated volume of effluent foam was increasing with increased rearing density. As increasing rearing density from $2\%$ to $7\%$, the protein concentration in rearing tank was increased from 16.6 g/$m^3$ to 21,9 g/$m^3$ and the removal amount of protein through foam separator as increased from 0.99 g/day to 2.5 g/day. But protein concentration ratio in the foam was decreased from 3.2 to 1.9. Changes of the removal amount and the concentration ratios of total suspended solid (TSS) and chemical oxygen demand (COD_cr.) were similar to proteins. The highest concentration ratios of TSS and COD_cr. were 10.2 and 8.4 at 2$2\%$ of rearing density.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.244-248
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• 2013

This study was carried out to investigate the residual characteristics and calculate processing factor of the environment friendly material matrine in fresh chilli pepper by drying. Spray solution of matrine was prepared by dilution of the commercial product (2% active ingredient) with water at 1 : 1000 (v/v) ratio and sprayed onto chilli pepper plants at seven day intervals. Samples were collected at 0, 1, 3, 5 and 7 days after last application and then dried using a hot air dry oven at $60^{\circ}C$ for 36 hours until the water content was reduced to 14%. Recoveries and storage period stabilities of matrine in the samples ranged from 106.6 to 119.1% and 106.6 to 113.1%, respectively. The residual concentrations of matrine in fresh chilli pepper and dried chilli peppers treated only once were found to be from less than 0.01 to 0.11 and from 0.03 to 0.25 mg/kg, respectively. In case of plants sprayed twice with matrine, the residual concentrations ranged from 0.02 to 0.12 and from 0.04 to 0.4 mg/kg, respectively. Processing factor of matrine in the fresh chilli pepper by drying was found to be from 1.5 to 3.3, indicating that the residual concentration of matrine in dried chilli pepper increased about two or three times by drying.

• Horticultural Science & Technology
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• v.32 no.4
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• pp.499-506
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• 2014

This research was conducted to develop root media containing ground and aged pine bark (GAPB) and ground and raw pine bark (GRPB). After analysis of physico chemical properties, the pine barks were blended with peat moss (PM) or coir dust (CD) in various ratios to formulate 12 root media. Then, two out of 12 root media were chosen based on the physical properties for further experiments. The pre-planting nutrient charge fertilizers (PNCF) were incorporated into two root media and chemical properties were analysed again. The total porosity (TP), container capacity (CC), and air-filled porosity (AFP) of GAPB were 78.7%. 39.4%, and 38.3%, respectively, while those of GRPB were 74.7%, 41.2%, and 33.4%, respectively. The percentage of easily available water (EAW, from CC to 4.90 kPa tension) and buffering water (BW, 4.91-9.81 kPa tension) in GAPB were 12.7% and 8.5%, respectively, which were a little lower than the 13.5% and 8.8% in GRPB. The pH and EC were not different significantly, but cation exchange capacity was different between the two pine barks (GAPB: pH 5.26, EC $0.61dS{\cdot}m^{-1}$, CEC $15.7meq{\cdot}100g^{-1}$; GRPB: pH 5.19, EC $0.32dS{\cdot}m^{-1}$, CEC $9.32meq{\cdot}100g^{-1}$). The concentrations of exchangeable cations in GAPB were Ca 0.32, K 0.05, Mg 0.27 and $0.12cmol+{\cdot}kg^{-1}$, whereas those in GRPB were Ca 0.28, K 0.08, Mg 0.25 and $0.09cmol+{\cdot}kg^{-1}$. The concentrations of $PO_4$-P, $NH_4$-N and $NO_3$-N were 485.8, 0.62 and $0.91mg{\cdot}L^{-1}$ in GAPB and 578, 1.00 and $0.82mg{\cdot}L^{-1}$ in GRPB, respectively, when those were analyzed in the solution of the saturated paste. The TP, CC and AFP in the two selected media were 89.3 and 76.3, and 13.0% in PM+GAPB (8:2, v/v) and 88.2, 68.2 and 20.0% in CD+GRPB (8:2), respectively. The pHs and ECs were 3.8 and $0.24dS{\cdot}m^{-1}$ in PM+GAPB which were a little lower than 5.8 and $0.65dS{\cdot}m^{-1}$ in CD+GRPB. However, the pHs analysed before and after incorporation of PNCF in the two root media did not show large differences. This is because the solubility of dolomitic lime is very low, and the pH it is expected to rise gradually when crops are cultivated int he root media. The information obtained in this study should facilitate effective formulation of root media containing pine bark.

• The Journal of Engineering Geology
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• v.21 no.2
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• pp.133-145
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• 2011

Unsaturated soil column tests were performed for weathered gneiss soil and weathered granite soil to assess the relationship between infiltration velocity and rainfall condition for different rainfall durations and for multiple rainfall events separated by dry periods of various lengths (herein, 'rainfall break duration'). The volumetric water content was measured using TDR (Time Domain Reflectometry) sensors at regular time intervals. For the column tests, rainfall intensity was 20 mm/h and we varied the rainfall duration and rainfall break duration. The unit weight of weathered gneiss soil was designed 1.21 $g/cm^3$, which is lower than the in situ unit weight without overflow in the column. The in situ unit weight for weathered granite soil was designed 1.35 $g/cm^3$. The initial infiltration velocity of precipitation for the two weathered soils under total amount of rainfall as much as 200 mm conditions was $2.090{\times}10^{-3}$ to $2.854{\times}10^{-3}$ cm/s and $1.692{\times}10^{-3}$ to $2.012{\times}10^{-3}$ cm/s, respectively. These rates are higher than the repeated-infiltration velocities of precipitation under total amount of rainfall as much as 100 mm conditions ($1.309{\times}10^{-3}$ to $1.871{\times}10^{-3}$ cm/s and $1.175{\times}10^{-3}$ to $1.581{\times}10^{-3}$ cm/s, respectively), because the amount of precipitation under 200 mm conditions is more than that under 100 mm conditions. The repeated-infiltration velocities of weathered gneiss soil and weathered granite soil were $1.309{\times}10^{-3}$ to $2.854{\times}10^{-3}$ cm/s and $1.175{\times}10^{-3}$ to $2.012{\times}10^{-3}$ cm/s, respectively, being higher than the first-infiltration velocities ($1.307{\times}10^{-2}$ to $1.718{\times}10^{-2}$ cm/s and $1.789{\times}10^{-2}$ to $2.070{\times}10^{-2}$ cm/s, respectively). The results reflect the effect of reduced matric suction due to a reduction in the amount of air in the soil.