• Archives of Pharmacal Research
• /
• v.28 no.10
• /
• pp.1147-1151
• /
• 2005

A new naturally occurring sterol, compound 5, and six known stigmasterols were isolated from fruits of Ailanthus altissima Swingle by repeated column chromatography and RP-HPLC. Their structures were identified as, 5${\alpha}$-stigmastane-3,6-dione (1), 3${\beta}$-hydroxystigmast-5-en-7-one (2), stigmast-5-ene-3${\beta}$, 7${\alpha}$-diol (3), 6${\alpha}$-hydroxystigmast-4-en-3-one (4), 5${\alpha}$-stigmastane-3${\beta}$, 6${\beta}$-diol (5), stigmast-4-ene-3${\beta}$, 6${\alpha}$-diol (6), stigmast-5-ene-3${\beta}$, 7${\alpha}$, 20$\xi$-triol (7) by spectral analysis and comparison with the published data. These compounds have not been reported from genus Ailanthus, whereas compound 7 was identified by NMR for the first time. In addition, the $95\%$ ethanol extract and compounds from the fruits of Ailanthus altissima SWINGLE were assayed for in vitro antimicrobial activity. The extract was potent active against the assayed bacteria while compounds 3 and 7 exhibited moderate activity.

• Korean Journal of Pharmacognosy
• /
• v.34 no.1 s.132
• /
• pp.28-32
• /
• 2003

In order to search for the anti-HIV agents from natural products, eighty MeOH extracts of medicinal plants were applied to a syncytia formation inhibition assay which is based on the interaction between the HIV-1 envelope glycoprotein gp120/gp41 and the cellular membrane protein CD4 of T lymphocytes. Among them, Ailanthus altissima showed a potent virus-cell fusion inhibitory activity. Repeated column chromatoghaphy of the methylene chloride fraction of A. altissima afforded compounds 1$({\beta}-sitosterol-3-O-{\beta}-D-glucoside)$, 2(tetramethoxycoumarin), and 3(ocotillone). Virus-cell fusion inhibitory activity of compound 3(ocotillone) was $70.76{\pm}4.09%$ at the concentration of $100\;{\mu}g/ml$.

• Proceedings of the PSK Conference
• /
• 2003.04a
• /
• pp.264.1-264.1
• /
• 2003

In order to search for the anti-HIV agents from natural products, Eighty MeOH extracts of medicinal plants were applied to a syncytia formation inhibition assay which is based on the interaction between the HIV-1 envelope glycoprotein gp120/gp41 and the cellular membrane protein CD4 of T lymphocytes. Among them, Ailanthus altissima showed a potent virus-cell fusion inhibitory activity. (omitted)

• Animal cells and systems
• /
• v.10 no.1
• /
• pp.27-40
• /
• 2006

Tree of heaven (Ailanthus altissima Swingle) as an invasive alien plant, appeared usually in the disturbed locations such as road-side, incised slope, and trampling path-side. They appeared abundantly in the trampling pathside but they did not appear or were rare in the interior of forest. Density and importance value of tree of heaven were proportionate to the relative light intensity measured according to distance from the trampling path toward forest interior and closely related to the breadth of trampling path as well. They were associated with annual, other exotic species or ruderal plants well. Distributional pattern of mature trees of them in the study area and its surrounding environments implied that they were introduced intentionally. Size class distribution of them showed that they are in expansion and artificial interferences such as, installing physical training space and developing hiking course functioned as trigger factors in their invasion and expansion. The results support the facts known generally in relation to invasion and expansion of the invasive alien plants. In this viewpoint, we suggest a management plan that applies ecological restoration principles to address ecosystems infected with tree of heaven by restoring the integral feature of the degraded nature and conserving the remained nature more thoroughly.

• Current Research on Agriculture and Life Sciences
• /
• v.10
• /
• pp.137-145
• /
• 1992

An efficient transformation system was established for Ailanthus altissima utilizing the binary system of A. tumefaciens strain LBA4404. Callus was initiated from small portions of cambium tissue of A. altissima in vitro. Optimum regeneration was achieved with Murashige and Skoog(MS) medium containing 0.01mg/${\ell}$ 2, 4-D, 0.5mg/${\ell}$ BAP, 3%(w/v) sucrose and 0.75% agar. The multiplication of explants remarkably showed up on medium containing 1.0mg/${\ell}$ BAP. Leaf discs or internodal stem segments were inoculated with A. tumefaciens strain LBA 4404 containing the binary vector pPMB 101, which has both ${\beta}$-glucuronidase (GUS) marker gene and neomycin phosphotransferase II (NPT II) gene. Shoots had been regenerated from 24 lines out of inoculative 50 lines. Transformants were selected by their ability to grow on medium containing kanamycin sulphate (100mg/${\ell}$). Putative transformation was confirmed by GUS assays. Five GUS-positive plantlets were obtained which confirmed that this marker gene has been transferred into A. altissima.

• Journal of Applied Biological Chemistry
• /
• v.56 no.4
• /
• pp.213-217
• /
• 2013

Phytochemical studies on the leaves of Ailanthus altissima (Simaroubaceae) have not been reported previously. Thus, the authors isolated and identified secondary metabolites from A. altissima. Dried and powdered leaves were extracted with 80% aqueous methanol, and the concentrated extract was successively partitioned with ethyl acetate, n-butanol, and water. Four flavonoids were isolated from the ethyl acetate fraction through repeated silica gel and octadecyl silica gel column chromatography. Spectroscopic data including NMR, MS, and IR allowed for identification of the chemical structures as quercetin (1), afzelin (2), quercitrin (3), and isoquercitrin (4). This is the first report of the isolation of these compounds from A. altissima. The four isolated flavonoids 1-4 as well as solvent fractions (ethyl acetate, n-butanol, and water), were evaluated for DPPH radical scavenging activity.

• Journal of Korean Society of Forest Science
• /
• v.78 no.4
• /
• pp.412-418
• /
• 1989

The stem segments of Ailanthus altissima were cultured on the Murashige & Skoog's medium(1962) supplemented with 0.1 mg/l BAP and 1.0 mg/l 2, 4-D for callus induction and proliferation, Shoot primordia were observed as greenish regions on the surface of yellow-brown calli about 8 weeks after culture. Shoot primordia were selected and transferred to the MS media containing various combination of BAP and 2, 4-D. Among these combinations the shoot primordia cell clusters on the medium added to 0.5mg/l BAP and 0.01mg/l 2, 4-D exhibited the highest number of shoot formation. These shoots were successfully transferred on the solid MS medium with no growth regulators for the rootings.

• Proceedings of the Zoological Society Korea Conference
• /
• 2003.08a
• /
• pp.174.4-174
• /
• 2003

No Abstract, See Full Text

• Journal of the Korean Wood Science and Technology
• /
• v.17 no.4
• /
• pp.1-25
• /
• 1989

가죽나무 (Alianthus altissima Swingle) 수간(樹幹)의 지항고(地上高) 180cm 부위(部位)에 19mm 너비의 플라스틱 밴드를 결체(結締)하여 1년간(年間) 방치(放置)한 후 이 밴드를 결체(結締)하여 주므로써 수가(樹幹)을 둘러싸는 원반형태(圓盤形態)의 과대(過大)한 비대생장(肥大生長) 조직(組織)인 종양상조직(腫瘍狀組織)(tumorous tissue)이 형성(形成)되고 이러한 종양상(腫瘍狀) 조직(組織)의 원반(圓盤)과 상하(上下)에 위치(位置)한 정상형태(正常形態)의 원반(圓盤)을 채취(採取)하여 해부학적(解剖學的) 차이(差異)를 조직학적(組織學的) 특성(特性)면에서 조사(調査) 비교(比較)하였다. 종양상(腫瘍狀) 부위(部位)에서는 이상적(異常的)으로 넓은 연륜폭(年輪幅)을 지니는 중양재(腫瘍材)(tumor wood)가 밴드 해체(解締) 다음 해인 제(第)3연륜(年輪)에서 형성(形成)되었으며 밴드 결체기간(結締期間)중에 생장(生長)한 제(第)2연륜내(年輪內)에서는 내부(內部)로 발달(發達)된 변색재(變色材)(discolored wood)와 외부(外部)로 발달(發達)한 정상재색(正常材色)을 구분하는 방어대(防禦帶)(barrier zone)가 형성(形成)되어 있었으며 이 종양상(腫瘍狀) 부위(部位)의 상하(上下)에 위치(位置)한 정상형태(正常形態)의 부위(部位)에서는 정상재색(正常材色)을 나타내는 제(第)2연륜내(年輪內)에 방어대(防禦帶)에 해당하는 위연륜상대(僞年輪狀帶)(false ring-like zones)가 형성(形成)되어 있었는데 이러한 방어대(防禦帶)와 위연륜상대(僞年輪狀帶)는 비정상적(非正常的)으로 과다한 이상형태(異常形態)의 유세포(柔細胞)와 상해(傷害)검구(溝) 및 검강(腔)으로 구성되어 있는 특징(特徵)을 나타내고 있다. 종양상(腫瘍狀) 부위(部位)에 있어서 종양재((腫瘍材)인 제(第)3연륜(年輪)과 방어대(防禦帶)후에 발달(發達)한 제(第)2연륜부분(年輪部分)은 불규칙(不規則)한 연륜계(年輪界), 매우 불규칙적(不規則的)인 구조(構造)를 지니는 이상조직(異常組織), 비정상적(非正常的)인 형태(形態)를 나타내는 목섬유(木纖維) 및 도관요소(導管要素), 비정상적(非正常的)인 형태(形態)를 지니는 방사조직(放射組織)을 가지며 추재부(秋材部)에 고립관공(孤立管孔) 및 복합관공(複台管孔)이 발달(發達)하며 스트랜드유세포(柔細胞)와 방사유세포(放射柔細胞)에 결정(結晶)이 존재(存在)한다는 면에서 서로 유사(類似)하였다. 그리고 제(第)3연륜(年輪)인 종양재(腫瘍材)의 춘재부(春材部) 관공(管孔)의 수(數)는 정상재(正常材)보다 작은 것으로 나타났다.

• Journal of Korean Society of Forest Science
• /
• v.78 no.3
• /
• pp.287-301
• /
• 1989

A tree of Ailanthus altissima Swingle was fastened with a plastic band, 19mm wide, around the stem 180cm above ground level and was left to grow under this condition for one year, By removal of this band the tumorous tissue gradually developed and the tree bearing distinct tumorous tissue, an overgrowth surrounding the stem, was harvested two years after the band removal. For the investigation of this tumorous part and its comparison with adjacent normal parts in the anatomical features of individual elements, the tumorous part and parts directly and 40cm above and below the tumorous part were obtained from the tree. The tumor wood having remarkably wider growth increment occurred in the 3rd growth ring the first year after removal of the fastened band, and the barrier zone which delimited the discolored wood from the normal-colored wood inwards appeared u1 the intra-2nd growth ring produced during the fastened period in the tumorous part and the false ring-like zones equivalent to barrier Zone were shown in the normal-colored 2nd growth rings of the parts directly and 40cm above and below the tumorous part, as well. The tumor wood, the 3rd growth ring, and proportion of the 2nd growth ring formed after barrier zone in the tumorous part shared common characteristics in the irregular growth ring boundary, misshapen and shorter individual fibers and vessel elements, and large ray widths and heights. The springwood pores were smaller in diameter in the tumor wood, and the larger radial and smaller tangential diameters of summerwood solitary pores and individual pores consisting of pore multiples in proportion of the 2nd growth ring formed after the barrier zone were transformed into near-isodiametric in the tumor wood, the 3rd growth ring, in the tumorous part. Only in proportion of the 2nd growth ring formed after the barrier zone were transformed into near-isodiametric in the tumor wood, the 3rd growth ring, in the tumorous part, ray densities greatly increased. And the massive tumor wood was caused not by cell size but by cell number because the radial and tangential diameters of fibers in the tumor wood, the 3rd growth ring, in the tumorous part were not sufficiently different from those in the same aged growth rings of the directly and 40cm above and below the tumorous part.