• 한국작물학회지
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• 제51권6호
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• pp.554-564
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• 2006

It is important to understand pedigree of rice cultivars commonly used for breeding. In this paper, pedigree tables for tracking the pedigree of 17 representative rice cultivars recommended by Rural development Adminstration (RDA) were completed and analyzed using two kinds of web database system; 'IRIS' and 'RRDB'. Seven cultivars, namely, 'Sangmibyeo', 'Ilpumbyeo', 'Saegewhabyeo', 'Surabyeo', 'Shindongjinbyeo', 'Ilmibyeo' and 'Jungwhabyeo' had 'Koshihikari' on the pedigree of their ancestor. Besides 'Koshihikari', the most feguently used ancestral germplasms among the high quality rice cultivars were 'Fujisaka 5', 'Kameno o' and 'Asahi', 'Fujisaka 5' was used as ancestral parent in 12 out of 17 cultivars. Interestingly, 'Kameno o' was used in pedigree of 16 out of 17 high quality varieties and 'Asahi' was used in the ancestral pedigree of all 17 varieties. 'Hwayeongbyeo' was used as one of parent in the breeding of 'Dongjin 1', 'Hwabongbyeo', 'Saegewhabyeo' and 'Junambyeo'. 'Ilpumbyeo' was used in the breeding pathway of 'Junambyeo' and 'Saegewhabyeo', 'Mangeumbyeo' itself was not enlisted as one of high quality rice cultivars, but was used as a breeding parent of three high quality varieties, namely, 'Saegewhabyeo', 'Hwabongbyeo' and 'Nampyeongbyeo'. Incorporated with evaluation data, pedigree will provide a valuable chance to genealogical tracking of agronomic traits such as disease resistance, grain quality and etc.

• 한국작물학회지
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• 제55권1호
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• pp.47-57
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• 2010

More than 2,500 accessions of squash germplasm are preserved in the National Agrobidiversity Center of RDA, Korea. We investigated the ecological and morphological characteristics on 100 accessions of the squash germplasms with a low viability. Two typical types of oriental squash (Cucurbita moschata) and western squash (Cucurbita maxima) are possessed 89 and 11 accessions, respectively. The origin of 100 accessions was divided into three groups, group I (Korea landraces with 81 accessions), group II (Nepal with 15 accessions) and group III (Russia, Uzbekistan, Laos, and Mexico with each one accession). Seventy eight accessions germinated at $27^{\circ}C$ in growth chamber. Days to first flowering of the female and male flower ranged from 32 days to 67 days and 24 days to 55 days after planting, respectively. Vine length showed the largest variation ranging from 357.8 cm to 1,465 cm, also the average length of ten stem node range has a variable ranges from 92 cm to 217.5 cm. All regenerated squash accessions have an only collided tendril and have three to seven lateral shoots. The flattened globe-shaped squash is the most predominant and exhibited ordinarily round shaped, ball shaped, long oblong-shaped, elongated shape, and pear shape. The average weight of fruit ranged from 1.33 kg to 7.95 kg. Sugar contents showed 5.4~12.6 $Brix^{\circ}$ without ripening period.

• 한국작물학회지
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• 제65권1호
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• pp.1-21
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• 2020

Association mapping is widely used in rice and other crops to identify genes underlying important agronomic traits. Most association mapping studies use diversity panels comprising accessions with various geographical origins to exploit their wide genetic variation. While locally adapted breeding lines are rarely used in association mapping owing to limited genetic diversity, genes/alleles identified from elite germplasm are practically valuable as they can be directly utilized in breeding programs. In this study, we analyzed genetic diversity of 179 rice varieties (161 japonica and 18 Tongil-type) released in Korea from 1970 to 2006 using 192 microsatellite markers evenly distributed across the genome. The 161 japonica rice varieties were genetically very close to each other with limited diversity as they were developed mainly through elite-by-elite crosses to meet the specific local demands for high quality japonica rice in Korea. Despite the narrow genetic background, abundant phenotypic variation was observed in heading time, culm length, and amylose and protein content in the 161 japonica rice varieties. Using these varieties in association mapping, we identified six, seven, ten, and four loci significantly associated with heading time, culm length, and amylose and protein content, respectively. The sums of allelic effects of these loci showed highly significant positive correlation with the observed phenotypic values for each trait, indicating that the allelic variation at these loci can be useful when designing cross combinations and predicting progeny performance in local breeding programs.

• 생명과학회지
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• 제8권4호
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• pp.431-436
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• 1998

잎 들깨 품종의 선발을 위하여 들깨 91품종의 집단에서 8개 형질에 대한 유전력 및 유전 상관을 추정하여 잎 들깨 양적 형질의 효율적 선발에 대한 유용한 정보를 얻기 위하여 본 연구를 수행하였던 바 그결과를 요약하면 다음과 같다. 유전력은 엽중, 엽면적, 엽장, 엽폭, 초장, 경직경, 마디수가 0.7311~09112로서 높았고, 1주엽수가 0.3241로서 다소 낮았다. 유전 상관은 엽중과 엽면적, 엽중과 엽장, 엽중과 엽폭, 엽중과 초장, 엽중과 경직성, 초장과 경직경, 초장과 마디수, 경직경과 마디수 사이에서 각각 +로 유의하였다. 모든 형질간의 상관관게어세 유전상관의 값이 표현형 상관의 값보다 높았다.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 1997년도 Progress and Future Development of Sericultural Science and Technology 40th Anniversary Commemoration Symposium
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• pp.23-49
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• 1997

This is a progress report of rice biotechnology including development of gene transformation system, gene cloning and molecular mapping in rice. The scope of the research was focused on the connection between conventional breeding and biotech-researches. Plant transformation via Agrobacterium or particle bombardment was developed to introduce one or several genes to recommended rice cultivars. Two chimeric genes containing a maize ribosome inactivating protein gene (RIP) and a gerbicide resistant gene (bar) were introduced to Nipponbare, a Japonica cultivar, and transmitted to Korean cultivars. The homozygous progenies of herbicide resistant transgenic plant showed good fertility and agronomic characters. To explore the genetic resourses in rice, over 8,000 cDNA clones from immature rice seed have been isolated and sequenced. About 13% of clones were identified as enzymes related to metabolic pathway. Among them, twenty clones have high homology with genes encoding enzymes in the photorespiratory carbon cycle reaction. Up to now about 100 clones were fully sequenced and registered at EMBL and GenBank. For the mapping of quantitative tarits loci (QTL) and eternal recombinant inbred population with 164 F13 lines (MGRI) was developed from a cross between Milyang 23 and Gihobyeo, Korean rice cultivars. After construction of fully saturated RFLP and AFLP map, quantitative traits using MGRI population were analyzed and integrated into the molecular map. Eighty seven loci were determined with 27 QTL characters including yield and yield components on rice chromosomes. Map based cloning was also tried to isolate semi-dwarf (sd-1) gene in rice. A DNA probe, RG 109, the most tightly linked to sd-1 gene was used to screen from bacterial artifical chromosome (BAC) libraries and five over lapping clones presumably containing sd-1 gene were isolated. Rice genetic database including results of biotech reasearch and classical genetics is provided at Korea Rice Genome Server which is accessible with world wide web (www) browser. The server provides rice cDNA sequences and map informations linked with phenotypic images.

• Plant Breeding and Biotechnology
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• 제5권3호
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• pp.237-242
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• 2017

AT-hook proteins of plant have shown to be involved in growth and development through the modification of chromatin architecture to co-regulate transcription of genes. Recently, many genes encoding AT-hook protein have been identified and their involvement in senescence delay is investigated. In this study, soybean transgenic plants overexpressing chromatin architecture-controlling ATPG7 gene was produced by Agrobacterium-mediated transformation and investigated for the positive effect on the important agronomic traits mainly focusing on yield-related components. A total of 27 transgenic soybean plants were produced from about 400 explants. $T_1$ seeds were harvested from all transgenic plants. In the analysis of genomic DNAs from soybean transformants, ATPG7 and Bar fragments were amplified as expected, 975 bp and 408 bp in size, respectively. And also exact gene expression was confirmed by reverse transcriptase-PCR (RT-PCR) from transgenic line #6, #7 and #8. In a field evaluation of yield components of ATPG7 transgenic plants ($T_3$), higher plant height, more of pod number and greater average total seed weight were observed with statistical significance. The results of this study indicate that the introduction of ATPG7 gene in soybean may have the positive effect on yield components.

• 한국육종학회지
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• 제43권5호
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• pp.383-390
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• 2011

Near-isogenic lines (NILs) carrying bacterial blight resistance genes (Xa4, xa5 and Xa21) were developed in japonica rice using Suweon345 as genetic background. NILs were selected by gene specific DNA markers and inoculation of K1 or K3a race. NILs conferring Xa4 were resistant to K1, K2, K3, and moderately resistant to K3a. NILs conferring xa5 were resistant to K1, K2, K3, and K3a. NILs having Xa21 were susceptible to K1, while resistant to K2, K3 and K3a. Target genes of NILs with the genetic background of Suweon345 were also confirmed by using eleven Philippines races and International Rice Bacterial Blight (IRBB) NILs carrying Xa4, xa5 and Xa21. All NILs had no significant difference from their recurrent parents in the major agronomic traits except for panicle length and brown rice 1,000 grain weight. Heading date of NILs ranged from Aug. 10 to Aug. 11, which was similar to that of recurrent parent, Suweon345. Culm length, number of grains per panicle and ratio of ripened grain of NILs were similar to those of Suweon345. Milled rice of NILs was ranged from 4.82 to 4.93MT/ha. These NILs will be useful for improving resistance to K3a race of bacterial blight pathogens in Korean japonica cultivars.

• 한국자원식물학회지
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• 제31권6호
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• pp.714-718
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• 2018

A new Gomchwi cultivar 'Sammany' was developed by a cross between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). Gomchwi is a common Korean name referring wild edible plant species within Ligularia genus. 'Sammany' has purple colored petiole ears and petiole trichome is absent. It has 2nd degree leaf vein density. Plant height, leaf length, leaf width and petiole length were 46.2, 19.1, 19.5 and 32.1 cm, respectively. Plant height was higher than 'Gondalbi'. Bolting occurred in mid. July and it flowered from late August to early September. 'Gondalbi' bolted and flowered 26 days earlier than 'Sammany', and consequently has earlier flowering time more than 26 day. Leaf number of 'Sammany' was 156 per plant but 'Gondalbi' had 130. 'Sammany' had thicker leaves (0.61 mm) compared to 'Gondalbi' (0.46 mm). As a result, yield was higher in 'Sammany (1,077 g/plant)' than 'Gondalbi (798 g/plant)' and leaf hardness was lower in 'Sammany ($20.8kg/cm^2$)' compared to 'Gondalbi ($23.0kg/cm^2$)'. In addition, 'Sammany' was found to be moderately resistant to powdery mildew. With enhanced agronomic and pathology traits, 'Sammany' was newly registered as a new Gomchwi cultivar (variety protection no. 131 on May 2017).

• 한국작물학회지
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• 제25권2호
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• pp.31-37
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• 1980

1. 간장에서 소맥의 Ephrat, Combine, Rousalka 대맥의 하가네무기, 무사시노무기, 영월륙각 과맥의 장기조생간, Wasehadaka, CI 09942, Liusanchia 01 등이 비교적 단간이고 일장변화에도 둔감한 품종이 있다. 2. 출수일수는 품종간 차이는 있으나 모두 단일에 의하여 출수기가 늦어졌고 소맥의 Sava 만이 12시간 일장에 비해 8시간에서 빨라졌다. 3. 성숙일수는 수맥의 중국8001 대맥의 올보리, 하가네무기, 경003, 수원006 과맥의 Kochihadaka, Wasehadaka, 장기한생과, 예산29호 등이 숙기도 빠르고 일장변화에도 안정된 품종이었다. 4. 일수입수는 품종별로 일정한 경향은 없으나 소맥의 원광, 남광, Blueboy, 수원 169호 대맥의 제천005, 시요기무기, Reno 과맥의 백동, Mochihadaka, CI 09867, CI 0968, Tawungmia 02는 수입수도 많고 일장변화에도 안정된 품종이었다. 5..주당수수는 소맥의 신광, Benhur, 관취기6006, 육성003는 단일에 의하여 수수가 증가하였고그 외에는 12시간 일장일 때에 가장 높고 24시간과 8시간에서 낮았으며 대맥은 제천005, 늘보리의 주당수수가 높았으며 Sava, Moldova는 8시간 일장일 때의 수수가 많았다. 과맥중 재래육각은 단일에 의한 증가폭이 컸으나 그 외의 품종은 단일에 둔감하였다. 6. 주당수량은 품종별로 일정한 경향은 없으나 소맥의 Littotio는 12시간에서 수량이 높았고 대맥의 제천005, 하가네무기, 삼척3006, CI 07296, 시요기무기 등이 안정된 높은 수량을 보였으며 과맥은 재래육각, Tawungmia 02, CI 09867은 수량이 높았다.

• 한국작물학회지
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• 제30권3호
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• pp.294-300
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• 1985

본 실험은 강보리·올보리·수원 18호 3개 정종을 공시하여 24시간 장일의 온실조건과 자연조건에서 지상부와 지하부의 형태변화를 비교·조사함으로써 맥류 육종에서의 세대단축재배에 활용할 수 있는 기초자료를 얻기 위하여 실시하였으며 그 결과는 다음과 같다. 1. 생육초기의 지상부 건물중은 자연조건에 비하여 온실조건에서 급격히 증가하고, 출수후에는 반대로 온실조건에서 낮았다. 2. 주당 엽면적은 자연조건에서 컸고, 근의 산화력은 온실조건에서 높았다. 3. 지하부의 건물중은 자연조건에 비하여 온실조건에서 현저히 작았고 지하부 건물중이 최대에 이르는 시기는 자연조건에서는 출수 4주후, 온실조건에서는 출수 7 주후였다. 4. 지하부에 대한 지상부의 비율(T/R ratio) 은 온실조건에서는 생육이 진전됨에 따라 계속 증가하는 경향이었고, 자연조건에서는 분얼개시기 까지는 감소하다가 그 이후 성숙기 까지는 증가하는 경향이었다.