Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Development of Near-Isogenic Lines (NILs) Conferring Xa4, xa5 and Xa21 Genes Resistant to Bacterial Blight (Xanthomonas oryzae pv. oryzae) in japonica rice Genetic Background

  • Kim, Ki-Young (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Shin, Mun-Sik (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Kim, Woo-Jae (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Park, Hyun-Su (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Ko, Jong-Cheol (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Nam, Jeong-Kwon (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Shin, Woon-Chul (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Mo, Young-Jun (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Jeung, Ji-Ung (Rice Research Division, National Institute of Crop Science, RDA) ;
  • Kim, Bo-Kyeong (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Ko, Jae-Kwon (Department of Rice and Winter Cereal Crop, NICS, RDA)
  • Received : 2011.10.02
  • Accepted : 2011.12.16
  • Published : 2011.12.30
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Abstract

Near-isogenic lines (NILs) carrying bacterial blight resistance genes (Xa4, xa5 and Xa21) were developed in japonica rice using Suweon345 as genetic background. NILs were selected by gene specific DNA markers and inoculation of K1 or K3a race. NILs conferring Xa4 were resistant to K1, K2, K3, and moderately resistant to K3a. NILs conferring xa5 were resistant to K1, K2, K3, and K3a. NILs having Xa21 were susceptible to K1, while resistant to K2, K3 and K3a. Target genes of NILs with the genetic background of Suweon345 were also confirmed by using eleven Philippines races and International Rice Bacterial Blight (IRBB) NILs carrying Xa4, xa5 and Xa21. All NILs had no significant difference from their recurrent parents in the major agronomic traits except for panicle length and brown rice 1,000 grain weight. Heading date of NILs ranged from Aug. 10 to Aug. 11, which was similar to that of recurrent parent, Suweon345. Culm length, number of grains per panicle and ratio of ripened grain of NILs were similar to those of Suweon345. Milled rice of NILs was ranged from 4.82 to 4.93MT/ha. These NILs will be useful for improving resistance to K3a race of bacterial blight pathogens in Korean japonica cultivars.

Keywords

References

  1. Cheema K, Grewal N, Vikal Y, Sharma R, Lore JS, Das A, Bhatia D, Mahajan R, Gupta V, Bharaj TS, Singh K. 2008. A novel bacterial blight resistance gene from Oryza nivara maped to 38kb region on chromosome 4L and transferred to Oryza sativa L. Genet. Res., Camb. 90:397- 407. https://doi.org/10.1017/S0016672308009786
  2. Gu K, Yang B, Tian D, Wu L, Wang D, Sreekala C, Yang F, Chu Z, Wang GL, White FF, Yin Z. 2005. R gene expression induced by a type-III effector triggers disease resistance in rice. Nature 435:1122-1125. https://doi.org/10.1038/nature03630
  3. Jena KK, Jeung JU, Noh TH, Heu S, Suh JP, Kim KY, Shin MS, Reveche MY, Pamplona A, Vera Cruz CM, Kim YG, Brar DS. 2007. Molecular breeding for bacterial blight resistance in japonica rice. The 2nd International Conference on Bacterial Blight of Rice. Nanjing, China. pp. 71-76.
  4. Jeung JU, Heu SG, Shin MS, VeraCruz CM, Jena KK. 2006. Dynamics of Xanthomonas oryzae. pv oryzae populations in Korea and their relationship to known bacterial blight resistance genes. Phytopathology 96:867-875. https://doi.org/10.1094/PHYTO-96-0867
  5. Kauffman HE, Reddy APK, Hsien SPY, Meraca SD. 1973. An improve technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis. Rep. 57: 537-541.
  6. Kim KY, Shin MS, Kim BK, Ko JK, Noh TH, Ha KY, Ko JC, Kim WJ, Nam JK, Baek MG, Noh KI, Park HS, Baek SH, Shin UC, Mo YJ, Choung JI, Kim YD, Kang HJ, Kim CK, Hwang HG, Kim JK. 2009a. A mid maturing rice cultivar with high-quality and bacterial blight resistance 'Jinbaek'. Korean J. Breed Sci. 41(3):314-318.
  7. Kim KY, Shin MS, Kim WJ, Ko JC, Baek MG, Ha KY, Kim BK, Ko JK, Nam JK, Noh GI, Park HS, Noh TH, Noh JH, Cheong JI, Kim YD, Mo YJ, Kim CK. 2008. A new medium-maturing, 'Gangbaek' with resistance to bacterial blight. Korean J. Breed Sci. 40(4):443-446.
  8. Kim KY, Shin MS, Kim WJ, Mo YJ, Nam JK, Noh TH, Kim BK, Ko JK. 2009b. Effective combination of resistance genes against rice bacterial blight pathogen. Korean J. Breed Sci. 41(3):244-251.
  9. Kim KY, Shin MS, Vera Cruz, CM, Shin SH, Ko JC, Kim BK, Ko JK, Kim CK. 2007. Resistant response of rice varieties to Korean and Philippines' bacterial blight, Xanthomonas oryzae. pv oryzae. Korean J. Intl. Agri. 19 (3):141-146.
  10. Korinsak S, Sriprakhon S, Sirithanya P, Jairin J, Korinsak S, Vanavichit A, Toojinda T. 2009. Identification of microsatellite markers (SSR) linked to a new bacterial blight resistance gene xa33 (t) in rice cultivar 'Ba7'. Maejo Int. J. Sci. Technol. 3:235-247.
  11. Lee KS, Rasabandith S, Angeles ER, Khush GS. 2003. Inheritance of resistance to bacterial blight in 21 cultivars of rice. Phytopathology 93:147-152. https://doi.org/10.1094/PHYTO.2003.93.2.147
  12. Liu DO, Ronald PC, Bogdanove AJ. 2006. Xanthomonas oryzae pathovars: model pathogens of a model crop. Mol. Plant Pathol. 7:303-324. https://doi.org/10.1111/j.1364-3703.2006.00344.x
  13. Mew TW, Alvarez AM, Leach JE, Swings J. 1993. Focus on bacterial blight of rice. Plant Dis. 77:5-12. https://doi.org/10.1094/PD-77-0005
  14. Murray MG, Thopson WF. 1980. Rapid isolation of high molecular-weight plant DNA. Nucleic Acids Res. 8:4321- 4325. https://doi.org/10.1093/nar/8.19.4321
  15. Noh TH, Lee DK, Kang MH, Shin MS, Na SY. 2003. Identification of new race of Xanthomonas oryzae pv. oryzae (Xoo) in Korea. (Abstr.) Phytopathology 96 (suppl.):S66.
  16. Ogawa T, Yamamoto T, Khush GS, Mew TW. 1991. Breeding of near-isogenic lines of rice with single genes for resistance to bacterial blight pathogen (Xanthomonas campestris pv. oryzae). Japan J. Breed. 41:523-529.
  17. Ou SH. 1985. Rice Disease. Commonwealth Mycological Institute, Aberystwyth, England. pp. 70-74.
  18. Ronald PC, Albano B, Tabien R, Abenes L, Wu KS, McCouch S, Tanksley D. 1992. Genetic and physical analysis of rice bacterial blight disease resistance locus, Xa21. Mol. Gen. Genet. 236:113-120.
  19. Shin MS, Noh TW, Lee JK, Shin HT, Lee YM. 2000. Breeding of japonica near-isogenic lines for resistance to bacterial blight in rice. Korean J. Breed. 32(3):291-295.
  20. Shin MS, Park SZ, Shin HT, Cho SY. 1998. Inheritance of resistance to bacterial blight in tongil type rice cultivar 'Changseongbyeo'. Korean J. Crop Sci. 43(2):64-65.
  21. Shin MS, Park SZ, Shin HT, Lee SY. 1994. Breeding of near-isogenic lines for resistance to bacterial blight in rice. Korean J. Breed. 30(2):185-191.
  22. Song WY, Pi LY, Wang GL, Gardner J, Holston T, Ronald PC. 1997. Evolution of the rice Xa21 disease resistance gene family. Plant Cell 9:1279-1287.
  23. Suh JP, Noh TH, Kim KY, Kim JJ, Kim YG, Jena KK. 2009. Expression levels of three bacterial blight resistance genes against K3a race of Korea by molecular and phenotype analysis in japonica rice (O. sativa L.). J. Crop Sci. Biotech. 12(3):103-108. https://doi.org/10.1007/s12892-009-0103-y
  24. Sun X, Cao Y, Yang Z, Xu C, Li X, Wang S, Zhang Q. 2004. Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like protein. Plant J. 37:517-527. https://doi.org/10.1046/j.1365-313X.2003.01976.x
  25. Sun X, Yang Z, Wang S, Zhang Q. 2003. Identification of a 47kb DNA fragment containing Xa4, a locus for bacterial blight resistance in rice. Theor. Appl. Genet. 106:683-687.
  26. Wang GL, Song WY, Ruan DL, Sideris S, Ronald PC. 1996. The cloned gene, Xa21, confers resistance to multiple Xanthomonas oryzae pv. oryzae strains in transgenic plants. Mol. Plant-Microbe Interact. 9:850-855. https://doi.org/10.1094/MPMI-9-0850
  27. Yang D, Sanchez, Khush GS, Zhu Y, Huang N. 1998. Construction of a BAC contig containing the xa5 gene locus in rice. Theor. Appl. Genet. 97:1120-1124. https://doi.org/10.1007/s001220050999