• Plant Breeding and Biotechnology
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• 제7권3호
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• pp.186-199
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• 2019

We selected 68 Chinese maize inbred lines to understand the genetic diversity, population structure, and marker-trait associations for eight agronomic traits and 50 simple sequence repeats (SSRs) markers. In this study, effective traits, such as days of anthesis (DA), days of silking (DS), ear height (EH), plant to ear height ratio (ER), plant height (PH), and leaf width (LW) were divided into PC1 and PC2 by PCA analysis for maize inbred lines. Genetic diversity analysis revealed a total of 506 alleles at 50 SSR loci. The mean number of alleles per locus was 10.12. The averages of genetic diversity (GD) and polymorphic information content (PIC) values were 0.771 and 0.743, respectively. Based on a membership probability threshold of 0.80, the population structure revealed that the total inbred lines were divided into three major groups with one admixed group. A marker-trait association using Q + K MLM showed that nine SSR markers (bnlg1017, umc2041, umc2400, bnlg105, umc1229, umc1250, umc1066, umc2092, and umc1426) were related with seven agronomic traits. Among these SSR markers, eight SSR markers were associated with only one agronomic trait (DA, DS, ER, LL, LW, PH, and ST), whereas one SSR marker (umc1229) was associated with two agronomic traits (DA and ST). These results will help in optimizing the choice of inbred lines for cross combinations, as well as in selecting markers for further maize breeding programs.

• 한국자원식물학회지
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• 제17권3호
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• pp.304-313
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• 2004

본 연구는 교잡종 옥수수의 품종개발 및 옥수수의 생산능력 방법을 개선하기 위한 기초연구로 미국에서 도입된 유망자식계통들을 이용한 5개의 교배조합들 그리고 이들의 F$_1$ 및 F$_2$ 세대에서 6개의 농업형 질들에 대한 유전양상을 구명하였다. 공시된 5개의 교배조합들과 조사된 6개의 농업형질들에서의 잡종강세효과는 교배조합 및 농업형질들에 따라 다소 차이는 있었지만 교배조합들 중에서는 Mo17/B14A, Va85/B73조합들이 조사된 대부분의 형질들에서 가장 뚜렷한 잡종강세 효과를 나타내었으며 , 그리고 조사된 6개의 농업형질들 중에서는 종자생산량이 교배조합들 사이에서 가장 큰 잡종강세 효과를 나타내었다. 조사된 6개의 농업형질들 중에서 종자생산량은 5개의 교배 조합들 중에서 C103/ND203과 FR35/Oh43 조합을 제외한 나머지 조합들에서 자식약세 현상이 다른 형질들보다 가장 크게 나타났고, 그리고 이들 수량관련 형질들은 일부 교배조합들에서의 예외적 경우를 제외하면 모두 정의 방향으로 자식 약세 현상이 나타나는 것으로 관찰되었다. 공시된 5개의 교배조합들 중에서 Mo17/B14A, C103/ND203 조합들은 조사된 6개의 농업형질들 모두에서 50%이상의 비교적 높은 유전성을 나타내었고, 반면에 Va85/B73 조합은 100립 중을 제외한 모든 형질들에서, 그리고 FR35/Oh43 조합은 간장과 착수고를 제외한 모든 형질들에서 50% 이하의 낮은 유전력을 나타내었다. 그리고 조사된 6개의 농업형질들 중에서 이삭길이를 제외한 모든 형질들은 5개의 교배조합들 모두에서 평균 유전력이 50% 이상을 나타내었으므로 이러한 형질들은 옥수수의 교잡육종을 위한 중요한 선발형질들임을 나타내었다 이상의 결과에 의하면, 본 연구에 이용된 5개의 교배 조합들 중에서 대비 조합으로 쓰인 Mo17/B14A(수원19호)조합은 조사된 대부분의 형질들에서 비교적 높은 생육 및 수량특성을 나타내었으므로 옥수수의 계통선발에 의한 품종육성에 유용한 품종 및 자원인 것으로 확인되었다.

• Journal of Crop Science and Biotechnology
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• 제11권3호
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• pp.171-176
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• 2008

The agronomic traits, such as days to flowering and maturity, plant height, 100-seed weight and seed filling period, are quantitatively inherited and important characters in soybean(Glycine max L. Merr.). A total of 126 $F_5$ recombinant inbred lines(RILs) developed from the cross of PI 171451$\times$Hwaeomputkong were used to identify quantitative trait loci(QTLs) for days to flowering(FD), days to maturity(MD), plant height(PH), 100-seed weight(SW), number of branches(NB) and seed filling period(FP). A total of 136 simple sequence repeat(SSR) markers segregated in a RIL population were distributed over 20 linkage groups(LGs), covering 1073.9 cM of the soybean genome with the average distance between adjacent markers of 7.9 cM. Five independent QTLs were identified for FD, three for MD, two for PH, three for SW, one for NB and one for FP. Of these, three QTLs were related to more than two traits of FD, MD, PH, NB and FP and mapped near the same positions on LGs H and O. Thus, these traits could be correlated with biologically controlled major QTLs in this soybean RIL population.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2021년도 춘계학술대회
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• pp.99-99
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• 2021

• 한국잡초학회지
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• 제32권1호
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• pp.10-16
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• 2012

이 연구는 비타민 A 강화벼(GM 벼)와 모품종인 낙동 및 일반품종을 대조로 농업적인 생육특성과 잡초를 대상으로 유전자 전이 정도를 조사하였다. GM 벼의 농업적인 특성에서 모품종인 낙동과 차이를 보이지 않았으며, 우점 잡초군과 건물중에서 유의성이 보이지 않았다. GM 벼와 모품종인 낙동 재배구의 우점 잡초는 물달개비, 올방개, 좀개구리밥, 물피 등의 10여 종이었다. 잡초의 유전자 전이 정도를 PCR 분석 결과, GM 벼와 낙동 그리고 우점잡초 8종에서 유전자 전이가 나타나지 않았다. 그러므로 비타민 A 강화벼의 화분이 비래하여 비표적 다른 품종의 벼 또는 주변 잡초에 유전자 이동이 일어나 외래유전자가 함유된 잡초가 출현하는 경우는 거의 없을 것으로 사료된다.

• 생명과학회지
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• 제26권6호
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• pp.727-736
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• 2016

쌀은 인류가 가장 많이 먹고 있는 주식이다. 최근 백색미, 적색미, 갈색미, 녹색미 그리고 흑색미 등 다양한 품종들이 소비되고 있다. 고농도의 안토시아닌이 함유되어 있는 흑미는 영양가뿐만 아니라 암 발생 예방 효과가 있어 세계적으로 그 소비가 증가되고 있다. 유전학적으로 흑미는 cyanidin-3-O-glucoside (C3G)의 합성경로를 조절하는 두 개의 유전자 Pb와 Pp가 관여하는 Purple Pericarp (Prp) 유전형질에 의하여 종자과피의 색이 결정된다. 1921년 Nagai가 흑미의 유전현상을 일으키는 Prp 형질을 보고한 이 후 Prp 형질에 관여하는 유전자들과 그 기능 분석에 관한 많은 연구가 보고 되었으나 아직도 많은 부분이 밝혀지지 않았다. 그러나 안토시아닌의 함량이 높은 기능성 흑미의 소비는 증가하고 있는 반면 흑미는 일반 재배 벼에 비하여 상대적인 수확량이 매우 적어 농경제적인 문제가 있다. 본 논문에서는 흑미 종자가 성숙하는 과정에서 Prp 유전자의 유전현상의 특성과 안토시아닌 합성 경로 그리고 수확량에 관한 유전학적 관점에서 내용을 정리하였다.

• 한국약용작물학회지
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• 제23권2호
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• pp.132-137
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• 2015

This study was conducted to find out the variation in agronomic trait and chemical composition in the collected Perilla frutescens from China and Japan. From the results of growth investigation, the maximum number if branches was 26.7ea in Japan 134 line, followed by 25 nodes number in China 119 line. Among the different lines investigated, maximum number of panicle number (108.8) were observed in China 114 line. 1000 seed weight was maximum (4.12 g) in China 118 line. Flowering time of different collected lines varied significantly with average value of 175.5 days and the average line required for maturation of seedlings was 205.1 days. Plant height was the highest (248.9 cm) in China 107 line. Highest number of total picking leaves was 965ea, and the average picked period was 54 days. The major phenol compounds contained in Perilla frutescens showed wide variation for Syringic acid, Benzoic acid, Naringin, o-Coumaric acid, Myricetin, Naringenin and Hesperetin. Japan 139 line showed the highest level of total phenol contents ($8254.0{\mu}g/g$, dry weight).

• Molecules and Cells
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• 제37권2호
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• pp.149-160
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• 2014

Plant breeders have focused on improving plant architecture as an effective means to increase crop yield. Here, we identify the main-effect quantitative trait loci (QTLs) for plant shape-related traits in rice (Oryza sativa) and find candidate genes by applying whole genome re-sequencing of two parental cultivars using next-generation sequencing. To identify QTLs influencing plant shape, we analyzed six traits: plant height, tiller number, panicle diameter, panicle length, flag leaf length, and flag leaf width. We performed QTL analysis with 178 $F_7$ recombinant inbred lines (RILs) from a cross of japonica rice line 'SNU-SG1' and indica rice line 'Milyang23'. Using 131 molecular markers, including 28 insertion/deletion markers, we identified 11 main- and 16 minor-effect QTLs for the six traits with a threshold LOD value > 2.8. Our sequence analysis identified fifty-four candidate genes for the main-effect QTLs. By further comparison of coding sequences and meta-expression profiles between japonica and indica rice varieties, we finally chose 15 strong candidate genes for the 11 main-effect QTLs. Our study shows that the whole-genome sequence data substantially enhanced the efficiency of polymorphic marker development for QTL fine-mapping and the identification of possible candidate genes. This yields useful genetic resources for breeding high-yielding rice cultivars with improved plant architecture.

• Plant Biotechnology Reports
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• 제5권2호
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• pp.135-146
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• 2011

The objective of the present research was to map QTLs associated with agronomic traits such as days from sowing to flowering, plant height, yield and leaf-related traits in a population of recombinant inbred lines (RILs) of sunflower (Helianthus annuus). Two field experiments were conducted with well-irrigated and partially irrigated conditions in randomized complete block design with three replications. A map with 304 AFLP and 191 SSR markers with a mean density of 1 marker per 3.7 cM was used to identify QTLs related to the studied traits. The difference among RILs was significant for all studied traits in both conditions. Three to seven QTLs were found for each studied trait in both conditions. The percentage of phenotypic variance ($R^2$) explained by QTLs ranged from 4 to 49%. Three to six QTLs were found for each yield-related trait in both conditions. The most important QTL for grain yield per plant on linkage group 13 (GYP-P-13-1) under partial-irrigated condition controls 49% of phenotypic variance ($R^2$). The most important QTL for 1,000-grain weight (TGW-P-11-1) was identified on linkage group 11. Favorable alleles for this QTL come from RHA266. The major QTL for days from sowing to flowering (DSF-P-14-1) were observed on linkage group 14 and explained 38% of the phenotypic variance. The positive alleles for this QTL come from RHA266. The major QTL for HD (HD-P-13-1) was also identified on linkage group 13 and explained 37% of the phenotypic variance. Both parents (PAC2 and RHA266) contributed to QTLs controlling leaf-related traits in both conditions. Common QTL for leaf area at flowering (LAF-P-12-1, LAF-W-12-1) was detected in linkage group 12. The results emphasise the importance of the role of linkage groups 2, 10 and 13 for studied traits. Genomic regions on the linkage groups 9 and 12 are specific for QTLs of leaf-related traits in sunflower.

• 한국작물학회지
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• 제44권1호
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• pp.86-94
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• 1999

A set of rice recombinant inbred lines was developed from a cross between a Tongil type variety, Nonganbyeo, and an indica variety, BG276, by the single seed descent method. The number of the lines in the population was 272. All the agronomic characters studied except ADV (alkali-digestion value) showed continuous variation among the RILs, implying that their inheritance mode should be quantitative. The patterns of the variation in the RILs were either normal or skewed distribution. ADVs of RILs were segregated into two groups with 1:1 ratio, indicating that ADVs in this KIL population might be controlled by one major gene. Transgressive variations were also observed in all characters. Heritability values of the characters varied from 0.488 in brown/rough rice ratio to 0.895 in alkali-digestion value. In the analysis of genotypic and phenotypic correlations, the character of yield was positively correlated with 8 different agronomic characters. The number of panicles per hill was negatively correlated with culm length, panicle length, and number of spikelets per panicle. Grain length was positively correlated with grain width, grain thickness, grain length/width ratio, white belly, ADV, and amylose. However, grain length/width ratio was negatively correlated with grain width. White core was also negatively correlated with white belly and ADV.