• KSBB Journal
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• 제19권5호
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• pp.406-409
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• 2004

Matrix Assisted Laser Desorption ionization Time of Flight (MALDI-TOF) was used for enzymes identification related to B -1,3-glucan synthesis. Agrobacterium sp. ATCC31750 was cultivated with two stage Continuous Stirrer Tank Reactor (CSTR) and the cells were harvested and their protein profiles were analysed by two dimensional electrophoresis. The specific enzyme spot was treated with trypsin and ana lysed by MALDI-TOF to get peptide molecular weight. The peptide molecular weights were matched with Agrobacterium tumefacience's Data Base from the matrix science site, then could identify the avaliable key enzymes. In this study, we identified key metabolite of synthesis of beta-1,3-glucan, such as glucose-6-phosphate isomerase, phosphoglucomutase, B-1,3-glucan synthase and glucokinase, and we also identified uracil phosphoribocyl transferase and Ribosome recycling factor also.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.245-246
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• 2000

Agrobacterium sp.에 의해 생산되는 커들란은 2차 부산물이기 때문에 이의 생산을 증대시키기 위해서는 고농도 세포배양이 필요하다. 본 연구에서는 고농도 세포배양을 실현하기 위하여 탄소원 과 질소원을 제어 공급하는 방법을 이용하였다. 초기 탄소원의 농도가 20g/L일 때 24시간 내에 모두 소모되었으며 생산된 세포의 농도는 6g/L였다. 고농도의 탄소원을 이용하여 배양조내의 설탕의 농도가 10g/L 이상이 되도록 제어하는 방법을 사용하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.668-671
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• 2005

In this study, the full gene of the putative ${\beta}-1,3-glucan$ synthase catalytic subunit(gi:40556679) in Agrobacteriujm sp. ATCC31750 was cloned into E. coli BL21(DE). We found that putative ${\beta}-1,3-glucan$ synthase catalytic subunit full gene mutant(E. coli mutant FC) produced soluble glucan.instead of curdlan(insoluble glucan).

• 생명과학회지
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• 제16권6호
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• pp.938-941
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• 2006

Continuous fermentation process for the production of soluble glucan using mutant Agrobacterium sp. ATCC31750 has been developed in this research. When the concentration of soluble glucan was higher than 6 g/l, the viscosity of the fermented broth was too high and it needs complex separation process to separate from culture broth. Mathematical models which describe the cell growth and glucan production was developed and they kinetic parameters were estimated with experimental data. They are used for the optimization of continuous fermentation process and calculate optimal dilution rate for easy separation of glucan 4 g/l. With continuous fermentation, glucan production rate was increased 1.8 times more than that with batch fermentation.