KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Identification of Key beta-1,3-glucan Synthesis Enzymes in Agrobacterium sp. ATCC31750

Agrobacterium sp. ATCC31750에 대한 beta-l,3-glucan 합성 대사경로의 주요 단백질 검출

  • Kim Ryo Hwa (Department of Chemical Engineering, Chosun University) ;
  • Lee Jung Heon (Department of Chemical Engineering, Chosun University)
  • 김려화 (조선대학교 공과대학 화학공학과) ;
  • 이중헌 (조선대학교 공과대학 화학공학과)
  • Published : 2004.10.01
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Abstract

Matrix Assisted Laser Desorption ionization Time of Flight (MALDI-TOF) was used for enzymes identification related to B -1,3-glucan synthesis. Agrobacterium sp. ATCC31750 was cultivated with two stage Continuous Stirrer Tank Reactor (CSTR) and the cells were harvested and their protein profiles were analysed by two dimensional electrophoresis. The specific enzyme spot was treated with trypsin and ana lysed by MALDI-TOF to get peptide molecular weight. The peptide molecular weights were matched with Agrobacterium tumefacience's Data Base from the matrix science site, then could identify the avaliable key enzymes. In this study, we identified key metabolite of synthesis of beta-1,3-glucan, such as glucose-6-phosphate isomerase, phosphoglucomutase, B-1,3-glucan synthase and glucokinase, and we also identified uracil phosphoribocyl transferase and Ribosome recycling factor also.

Keywords

References

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