• 제목/요약/키워드: Agriculture technology

검색결과 7,123건 처리시간 0.032초

Sarsaponin Effects on Ruminal Fermentation and Microbes, Methane Production, Digestibility and Blood Metabolites in Steers

  • Lila, Zeenat Ara;Mohammed, Nazimuddin;Kanda, Shuhei;Kurihara, Mitsunori;Itabashi, Hisao
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제18권12호
    • /
    • pp.1746-1751
    • /
    • 2005
  • The objective of this study was to evaluate the effects of sarsaponin on methane production, ruminal fermentation, nutrient digestion and blood metabolites using three Holstein steers in a 3${\times}$3 Latin Square design. The steers were fed Sudangrass hay plus concentrate mixture at a ratio 1.5:1 twice daily, and sarsaponin (0, 0.5 and 1% of DM), which was given at 09:00 and 17:00 h daily by mixing with concentrate. Rumen samples were collected 0, 2, and 5 h after morning dosing. Ruminal pH was numerically decreased and numbers of protozoa were decreased linearly (p<0.01) by treatment. Ruminal ammonia-N was reduced (linear; p<0.05) and total VFA was increased (quadratic; p<0.05) at 2 and 5 h after sarsaponin dosing. The molar proportion of acetate was decreased (quadratic; p<0.05) and propionate was increased (linear; p<0.01) at all sampling times. Blood plasma glucose was increased and urea-N was decreased (linear; p<0.05) at 2 and 5 h after dosing. Methane was decreased by approximately 12.7% (linear; p<0.05). The apparent digestibility of DM and NDF were decreased (quadratic; p<0.05) and that of CP remained unchanged due to the sarsaponin. The numbers of cellulolytic bacteria were decreased (quadratic; p<0.05), while numbers of total viable bacteria remained unchanged due to the sarsaponin. These results show that sarsaponin can partially inhibit rumen methanogenesis in vivo and improve ruminal fermentation, which supports our previous in vitro results.

Laboratory and Field Evaluations of Entomopathogenic Lecanicillium attenuatum CNU-23 for Control of Green Peach Aphid (Myzus persicae)

  • Kim, Hyang-Yeon;Lee, Hyang-Burm;Kim, Young-Cheol;Kim, In-Seon
    • Journal of Microbiology and Biotechnology
    • /
    • 제18권12호
    • /
    • pp.1915-1918
    • /
    • 2008
  • An entomopathogenic fungus was isolated from an infected aphid. The isolate conformed most closely to Lecanicillium attenuatum CBS 402.78 (AJ292434) based on the internal transcribed spacer (ITS) region of its 18S rDNA, and thus was designated L. attenuatum CNU-23. Laboratory and field evaluations of CNU-23 blastospores were carried out for the control of green peach aphids. The laboratory evaluations of CNU-23 revealed an aphid mortality of about 80% with an estimated $LT_{50}$ of 3.72 days after the application of CNU-23 at $1{\times}10^6$ blastospores/ml. Meanwhile, the field evaluations of CNU-23 performed on greenhouse pepper plants during the rainy season showed an aphid mortality ranging from 72% to 97%. Significant sporulation was observed in the aphids treated with CNU-23. Therefore, the results suggest that L. attenuatum CNU-23 can be used as a biocontrol agent for green peach aphids on greenhouse pepper plants.

Immunohistochemical Localization of Endogenous IAA in Peach (Prunus persica L.) Fruit during Development

  • Zhang, Wei;Li, Yang;Shi, Mengya;Hu, Hao;Hua, Baoguang;Yang, Aizhen;Liu, Yueping
    • 원예과학기술지
    • /
    • 제33권3호
    • /
    • pp.317-325
    • /
    • 2015
  • Peach (Prunus persica L.) is a model species for stone fruit studies within the Rosaceae family. Auxin plays an important role in the development of peach fruit. To reveal the distribution of auxin in the tissues of peach fruit, immunohistochemical localization of IAA was carried out in the seed, mesocarp, and endocarp in developing peach fruit using an anti-indole-3-acetic acid (anti-IAA) monoclonal antibody. A strong IAA signal was observed throughout the outer and inner integument during peach fruit development, and the distribution was zonal. The IAA signal was mainly focused in mucilage layers in the outer integument. The outer integument may function to produce or store IAA in the seed; a strong IAA signal was detected in the cells around the vascular tissue, whereas a weak IAA signal was located in the vascular tissues. In the mesocarp, the cells around the vascular bundle tissue gave rise to an IAA signal that increased in the late phase of fruit growth, which coincided with a significant increase in fruit growth. The distribution of IAA, however, was changed when fruit was treated with auxin transport inhibitors NPA (1-N-naphthylphthalamic acid) or TIBA (2, 3, 5-triiodobenzoic acid); in mesocarp tissues, an IAA signal was detected mainly in vessels of the treated fruit. During the critical period of endocarp lignification, the vessel lignification process was negatively correlated with IAA signal. The present results confirmed that the distribution of IAA was different in various tissues of peach fruit according to the developmental stage. This research provides cytological data for further study of the regulatory mechanism of auxin in peach fruit.

곤충유래 키틴/키토산의 구조특성 (Structural Characteristics of Insect Chitin/Chistosan)

  • 이광길;이용우;권해용;여주홍;박인규;남진;설광열
    • 한국잠사곤충학회지
    • /
    • 제40권2호
    • /
    • pp.158-162
    • /
    • 1998
  • The chitin was isolated from various kinds of insects such as exuvia of Psacothea hilaris Pascoe, silkworm pupa, Agrius convolvuli or from cuticle of cockroach by treatment with dilute HCI and NaOH. The chemical and crystalline structure was characterized by FT-IR and X-ray diffractometer. All of the chitins extracted from insects showed characteristic ${\alpha}$-chitin peaks at the Bragg angle 2$\theta$=9.3$^{\circ}$, 19.4$^{\circ}$, and 23.5$^{\circ}$by X-ray diffraction analysis. The transition from chitin to chitosan was confirmed by IR spectra and the degree of deacetylation of the crab shell, silkworm pupa, cockroach, and Psacothea hilaris Pascoe was 70.9, 76.4, 75.5, and 74.1%, respectively. The double diffraction peaks of insect chitosan were observed at 2$\theta$=10$^{\circ}$and 20$^{\circ}$, indication the characteristic of hydrated crystalline structure of chitosan.

  • PDF

발수제를 적용한 농산물 재배용지의 제조 (Application of Agricultural Cultivation Paper Treated with Water Repellent)

  • 김강재;정진동;안은별;엄태진
    • 펄프종이기술
    • /
    • 제47권4호
    • /
    • pp.102-108
    • /
    • 2015
  • Agricultural cultivation papers treated with water repellent were investigated in order to analyse mechanical properties and durability. The water repellency was optimal when papers are treated with Wb and Sa water repellents. Also, WCPs with 5% water repellent were more effective than those of 3 and 10%. In addition, water repellency of WCPs with Wb and Sa water repellents were kept constant in correlation with aging time. Finally, WCP with Wb water repellent possesses efficiency in terms of mechanical properties and durability.