• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.122-122
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• 2003

This study examined the viability of canine oocytes following storage at 4 or $38{\circ}C$ for 5 hrs. The ovaries were collected from domestic dog following ovariohysterectomy at a local veterinary clinics and transported to laboratory In two different transport temperature at 4 or $38{\circ}C$ within 5 hrs. The cumulus oocyte complexes (COCs) were recovered after slicing with blade. In Exp. 1, the oocytes collected were matured in DMEM supplemented with l0% FBS, 0.6 mM/mlcysteine, 0.2 mM Pyruvic acid, 20 ng/ml $E_2$ and 1 $\mu g/ml$ rbST at humidified atmosphere containing 5% $CO_2 38{\circ}C$ for 24 or 48 hrs to analysis of survivability. In Exp 2, to assess nuclear development at 38{\circ}C$ group, the oocytes were matured in maturation medium for 24, 48 or 96 hrs. Survivability was judged by a morphological appearance and PI staining. Survivability rates were analyzed by General Linear Models procedure in SAS. The survival rates at 4{\circ}C$ ovary transport group showed significantly lower than at 38{\circ}C$ group (0 vs 72.9% in 48 hrs and 13.2 vs 77 8% in 24 hrs; P<0.05). The nuclear development of oocytes to MI to MII stages at 24, 48 and 96 hrs was 8.3% (6/72), 8.9% (9/101), and 9.5% (8/84). These results showed that the canine oocytes were remarkably sensitive to a low temperature and did not increase nuclear development rate depend on maturation time to 96 hrs.

• Asian-Australasian Journal of Animal Sciences
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• 제25권8호
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• pp.1089-1095
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• 2012

Neonatal Fc receptor (FcRn) gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G (IgG) and is responsible for IgG transport and stabilization. In this report, the 8,900 bp porcine FcRn genomic DNA structure was identified and putative FcRn protein included 356 amino acids. Alignment and phylogenetic analysis of the porcine FcRn amino acid sequences with their homologies of other species showed high identity. Tissues expression of FcRn mRNA was detected by real time quantitative polymerase chain reaction (Q-PCR), the results revealed FcRn expressed widely in ten analyzed tissues. One single nucleotide polymorphism (SNP) (HQ026019:g.8526 C>T) in exon6 region of porcine FcRn gene was demonstrated by DNA sequencing analysis. A further analysis of SNP genotypes associated with serum Classical Swine Fever Virus antibody (anti-CSFV) concentration was performed in three pig populations including Large White, Landrace and Songliao Black pig (a Chinese indigenous breed). Our results of statistical analysis showed that the SNP had a highly significant association with the level of anti-CSFV antibody (At d 20; At d 35) in serum (p = 0.008; p = 0.0001). Investigation of expression and polymorphisms of the porcine FcRn gene will help us in further understanding the molecular basis of the antibody regulation pathway in the porcine immune response. All these results indicate that FcRn gene might be regarded as a molecular marker for genetic selection of anti-CSFV antibody level in pig disease resistance breeding programmes.

• Asian-Australasian Journal of Animal Sciences
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• 제26권4호
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• pp.463-469
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• 2013

Cluster of differentiation 4 (CD4) is mainly expressed on $CD4^+$ T cells, which plays an important role in immune response. The aim of this study was to detect the association between polymorphisms of the CD4 gene and T lymphocyte subpopulations in pigs, and to investigate the effects of genetic variation on the CD4 gene expression level in immune tissues. Five missense mutations in the CD4 gene were identified using DNA pooling sequencing assays, and two main haplotypes (CCTCC and AGCTG) in strong linkage disequilibrium (with frequencies of 50.26% and 46.34%, respectively) were detected in the population of Large White pigs. Our results indicated that the five SNPs and the two haplotypes were significantly associated with the proportions of $CD4^-CD8^-$, $CD4^+CD8^+$, $CD4^+CD8^-$, $CD4^+$ and $CD4^+/CD8^+$ in peripheral blood (p<0.05). Gene expression analysis showed the mRNA level of the CD4 gene in thymus was significantly higher than that in lymph node and spleen (p<0.05). However, no significant difference was observed between animals with CCTCC/CCTCC genotype and animals with AGCTG/AGCTG genotype in the three immune tissues (p>0.05). These results indicate that the CD4 gene may influence T lymphocyte subpopulations and can be considered as a candidate gene affecting immunity in pigs.

• 한국유기농업학회지
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• 제11권2호
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• pp.1-21
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• 2003

Customary agriculture seeks to increase production and supply people with safe foods. Thus. the promotion and establishment of organic agriculture are required to reduce water and soil pollution caused by customary agriculture. Although organic agriculture is an agricultural technology system whose basic principle is organic water circulation in agronomic agriculture and livestock industry. the livestock raising sector has not been developed in Korean organic agriculture: hence the limited development of agronomic agriculture. This study therefore sought to develop a standardized model connected with organic livestock raising and organic agronomic agriculture to secure symmetric and continued development. Specifically, this study reviewed the technological and economic problems related to the development of a naturally circular standard model where organic agronomic agriculture and organic livestock raising are connected. Likewise, a model for calculating the appropriate quantity of fertilizers to be applied and appropriate number of livestock to be bred was proposed as important factors in the development of a regionally circular agriculture model, and an alternative to a system connecting the two factors suggested.

• Journal of the Korean Wood Science and Technology
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• 제39권5호
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• pp.398-405
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• 2011

Nondestructive evaluation (NDE) technique method using a resonance frequency mode was carried out for woodceramics made by different phenol resin impregnation ratios (40, 50, 60, 70%) for Broussonetia Kazinoki Sieb. Dynamic modulus of elasticity increased with increasing resin impregnation ratios. There was a close relationship between dynamic modulus of elasticity and static bending modulus of elasticity and between dynamic modulus of elasticity and MOR and between static bending modulus of elasticity and MOR. Therefore, the dynamic modulus of elasticity using resonance frequency mode is useful as a nondestructive evaluation method for predicting the MOR of woodceramics made by different impregnation ratios.

• Agribusiness and Information Management
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• 제4권1호
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• pp.32-40
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• 2012

In recent times, NFC technology adaptations for smartphones have been increasing. This study proposes the adaptation of agri-food business models based on NFC technology and presents the basic technological characteristics of NFC. An NFC tag can store more information than prior tagging technology methods, such as QR codes, and provides a better user experience. Based on the unique features of NFC, this study suggests an NFC business model application for the agri-food business.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2016년도 추계학술대회
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• pp.479-481
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• 2016

이 논문은 농업 비즈니스에서 발생하는 농산물 이벤트를 GS1 표준에서 정의하는 이벤트 구조로 정의하고, 표준 인터페이스로 저장/공유하는 정보 서비스를 제안한다. 이를 통해 농업 비즈니스 종사자들은 상이한 데이터 구조를 상호 운용하는 비용을 줄이고 최소한의 상호 이해만으로 정보를 주고받을 수 있을 것으로 예상한다.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.17-22
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• 2008

Aromatic rice has become popular owing to its aroma. Growing demand for aromatic rice has spurred interest in the development of domestic cultivars that offer similar combinations of grain attributes such as texture, cooking characteristics, aroma, and taste. In this study, the most important agronomic attributes and aroma of 26 cultivars from Afghanistan, Iran, and Uzbekistan, and controls from Japan, Thailand, and India were characterized. Also $F_2$ populations derived from the cross between(Jasmine 85 aromatic$\times$Nipponbare non-aromatic) and(Jasmine 85$\times$Basmati 370 aromatic) were obtained. Tasting individual grains, cooking test, 1.7% KOH sensory test, and molecular marker analysis have been applied to distinguish between aromatic and non-aromatic rice. Diversity for some traits of agronomic importance, such as plant height was detected among countries, e.g. Afghan cultivars classified as tall, and Iranian and Uzbek intermediate and short, respectively. Differentiations of panicle, grain, leaf, basal internode, and culm dimension among rice cultivars, indicating the source of rice diversity in Central Asia. According to the results, 6 of 10, 2 of 7, and 0 of 6 of Afghan, Iranian, and Uzbek rice cultivars were scored as aromatic, respectively. Therefore, Afghan cultivars are a good source of aromatic rice germplasm for Central Asia. The expression between aromatic and non-aromatic, and aromatic and aromatic combinations has been evaluated. The observed segregation ratio of these crosses in the $F_2$ populations was tested by $x^2$ analysis against the expected ratio for a single gene. A segregation ratio of 3:1 between non-aromatic and aromatic combination has been detected, while segregation has not been detected between the aromatic and aromatic combinations. Also, parallel results were obtained from the tested aromatic rice cultivars. Thus, our results suggest that a single recessive gene controls aroma in all aromatic rice cultivars.

• 산업경영시스템학회지
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• 제46권3호
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• pp.41-58
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• 2023

This study identifies the commercialization success factors that can be an important indicator for the transfer and commercialization of national R&D results in the agricultural sector. Unlike other industries, the agricultural sector has a non-systematically scaled and processed industrial structure, and R&D is led by government rather than the private sector. Although the quantitative performance of national agricultural R&D, especially the number of patents and publications, has increased rapidly with the quantitative expansion of the government R&D budget, the technology commercialization of the results of agricultural R&D has been accompanied by difficulties for SMEs. Therefore, this study summarized the success factors for commercialization of state-owned technologies presented in previous studies, and based on them, analysed the success factors for commercialization specific to the agricultural sector. It also conducted a questionnaire survey using Delphi and focus group interviews (FGI) with experts from academia, research and industry, and a survey of agricultural companies to derive success factors for commercialization in the agricultural sector using logistic regression analysis. As a result, five indicators with positive correlation and three indicators with negative correlation within technology characteristics, suppliers, adopters, policy and market factors were finally derived as key factors for agricultural commercialization. In the future, it is expected that independent factor analysis of the food and seed sectors, which have independent industry characteristics from the agricultural sector, will be needed.

• Animal Bioscience
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• 제37권6호
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• pp.1041-1052
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• 2024

Objective: Bacillus subtilis, a kind of probiotic with broad-spectrum antibacterial function, was commonly used in livestock and poultry production. Recent research suggested that Bacillus subtilis may have antioxidant properties and improve immune response. This study aimed to verify the probiotic function of Bacillus subtilis in the production of broiler chickens. Methods: A total of 324 (1-day-old) Arbor Acres broilers were selected and randomly divided into three groups: basal diet group (Ctr Group), basal diet + antibiotic growth promoter group (Ctr + AGP) and basal diet + 0.5% Bacillus subtilis preparation group (Ctr + Bac). The experiment lasted for 42 days. Muscle, serum and liver samples were collected at 42 days for determination. Results: The results showed that Bacillus subtilis could decrease malondialdehyde content in the serum and liver (p<0.05) and increase superoxide dismutase 1 mRNA expression (p<0.01) and total superoxide dismutase (p<0.05) in the liver. In addition, compared with AGP supplementation, Bacillus subtilis supplementation increased interleukin-10 (IL-10) and decreased tumor necrosis factor-α and IL-1β level in the serum (p<0.05). At 45 minutes after slaughter Ctr + Bac presented a higher a^* value of breast muscle than Ctr Group (p<0.05), while significant change in leg muscle was not identified. Moreover, there was no difference in weight, shear force, cooking loss and drip loss of breast and leg muscle between treatments. Conclusion: Our results demonstrate that Bacillus subtilis in diet can enhance antioxidant capacity and optimize immune response of broilers.