• Title/Summary/Keyword: Agriculture and Forestry

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Biocontrol of Late Blight (Phytophthora capsici) Disease and Growth Promotion of Pepper by Burkholderia cepacia MPC-7

  • Sopheareth, Mao;Chan, Sarun;Naing, Kyaw Wai;Lee, Yong Seong;Hyun, Hae Nam;Kim, Young Cheol;Kim, Kil Yong
    • The Plant Pathology Journal
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    • v.29 no.1
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    • pp.67-76
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    • 2013
  • A chitinolytic bacterial strain having strong antifungal activity was isolated and identified as Burkholderia cepacia MPC-7 based on 16S rRNA gene analysis. MPC-7 solubilized insoluble phosphorous in hydroxyapatite agar media. It produced gluconic acid and 2-keto-gluconic acid related to the decrease in pH of broth culture. The antagonist produced benzoic acid (BA) and phenylacetic acid (PA). The authentic compounds, BA and PA, showed a broad spectrum of antimicrobial activity against yeast, several bacterial and fungal pathogens in vitro. To demonstrate the biocontrol efficiency of MPC-7 on late blight disease caused by Phyto-phthora capsici, pepper plants in pot trials were treated with modified medium only (M), M plus zoospore inoculation (MP), MPC-7 cultured broth (B) and B plus zoospore inoculation (BP). With the sudden increase in root mortality, plants in MP wilted as early as five days after pathogen inoculation. However, plant in BP did not show any symptom of wilting until five days. Root mortality in BP was markedly reduced for as much as 50%. Plants in B had higher dry weight, P concentration in root, and larger leaf area compared to those in M and MP. These results suggested that B. cepacia MPC-7 should be considered as a candidate for the biological fertilizer as well as antimicrobial agent for pepper plants.

Effect of Nutrient Solution Ratio on Growth in ICT-Based Ginseng Process Ginseng Plant (ICT 기반의 인삼 공정 육묘 시 양액비율이 생장에 미치는 영향)

  • Dong Hyun, Kim;Yeon Bok, Kim;Hyun Jung, Koo;Hyun Jin, Baek;Su Bin, Lee;Eui Gi, Hong;Sang gi, Kim;Kwang Jin, Chang
    • Journal of Practical Agriculture & Fisheries Research
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    • v.24 no.4
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    • pp.5-10
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    • 2022
  • After ginseng seeds were planted in sand, the first experiment was conducted by germinating seeds with a nutrient concentration of 0 to 2.5. The average germination rate was highest in EC 1.0 with a nutrient concentration of 87%, followed by the comparative group with EC 1.5, 82%, EC 2.0, 78%, EC 2.5, 72%, EC 0.5, 71%, and con, 68%. Ginseng seeds were sown in the sandy soil, grown for 60 days were transferred to the ginseng soil, and the second growth experiment was conducted 30 days later. As a result of the experiment, at the nutrient concentration of EC 1.5, it grew from 11.64cm to 15.54cm, the average total length(cm)increased the most from 3.90cm. At the EC 1.0, nutrient concentration, the average total weight(g)increased the most from 0.42g to 0.75g to 0.33g. At the EC 0.5, nutrient concentration, the average total root width(mm) increased the most from 4.06mm to 5.52mm to 1.46mm.

Toward Establishment of Japan-Korea Long-Term Forest Hydrological Research Network

  • Otsuki, Kyoichi;Ogawa, Shigeru;Kume, Atsushi;Kumagai, Tomo'omi
    • Proceedings of the Korea Water Resources Association Conference
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    • 2002.05a
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    • pp.51-58
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    • 2002
  • In this paper, the status of forest and forestry together with the trend of forest hydrology in Japan are firstly overviewed for the mutual understanding between the Japan Society of Hydrology and Water Resources (JSHWR) and the Korean Water Resources Association (KWRA). Then, Long-Term Ecological Research recently introduced in Asia is briefly explained, and the establishment of Japan-Korea Long-Term Forest Hydrological Research Network is proposed.

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Quality Improvement of Oil Palm Trunk Properties by Close System Compression Method

  • Hartono, Rudi;Wahyudi, Imam;Febrianto, Fauzi;Dwianto, Wahyu;Hidayat, Wahyu;Jang, Jae-Hyuk;Lee, Seung-Hwan;Park, Se-Hwi;Kim, Nam-Hun
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.2
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    • pp.172-183
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    • 2016
  • Densification of the inner part of oil palm trunk (OPT) by the close system compression (CSC) method was performed in this study. The effects of the compression temperature and time on the anatomical, physical and mechanical properties of OPT were evaluated. The inner part of OPT with an initial average density of $0.3g/cm^3$ was used as samples. Oven-dried samples were immersed in water and vacuumed until fully saturated and then compressed by CSC at 120, 140, 160 or $180^{\circ}C$ for 10, 20, 30 or 40 min. The anatomical characteristics of transverse and radial sections before and after compression were compared by optical microscopy. The physical and mechanical properties, including the density, recovery of set (RS), modulus of elasticity (MOE), modulus of rupture (MOR), and compression parallel to grain were examined. It was observed that the anatomical characteristic of the inner part of OPT (i.e., vascular bundles, vessels, and parenchyma tissue) became flattened, fractured, and collapsed after compression by CSC. The RS decreased with increasing compression temperature and time. The lower RS indicated high dimensional stability. The physical and mechanical properties (i.e., density, MOR, MOE, and compressive strength) of the inner part of OPT increased with increasing compression temperature and time. Compression by the CSC method at $160^{\circ}C$ for 40 min was the optimum treatment.

Induced monoterpene and lignin production in mechanically stressed and fungal elicited cultured Cupressus lusitanica cells

  • De Alwis, Ransika;Fujita, Koki;Ashitani, Tatsuya;Kuroda, Ken'ichi
    • Plant Biotechnology Reports
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    • v.3 no.1
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    • pp.57-65
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    • 2009
  • Cultured Cupressus lusitanica cells induced by various stresses are thought to produce different complexes of defense chemicals to optimize defense. To compare the induced products of two stimulations, we investigated the emission of monoterpenes, biosynthesis of ${\beta}-thujaplicin$, and accumulation of lignin in mechanically stressed and fungal elicited cultured C. lusitanica cells. Both mechanical stress and fungal elicitor caused emission of qualitatively similar monoterpene blends indicating de novo biosynthesis of these compounds after stimulation, while mechanical stress alone is sufficient to induce fungal elicitor-related monoterpene emission. Sabinene and limonene were the dominant compounds over the time course in both volatile blends. Although the emitted volatile blends were qualitatively similar, the time course and the relative ratios of the constituents of the volatile blends differed with the type of stimulation. While fungal elicited cells produced significant amounts of ${\beta}-thujaplicin$ over the 5-day time course, no ${\beta}-thujaplicin$ was observed in the mechanically stressed cells. The production of ${\beta}-thujaplicin$ was the main dissimilarity of the induced products of these two treatments, suggesting that synthesis of ${\beta}-thujaplicin$ is not a general response to all types of stresses, but is a specific response and serves as a strong toxic compound against already invaded fungus. Significantly higher amounts of lignin accumulations were observed in the fungal elicited and mechanically stressed cells on the 5th day after induction. Based on these results, we suggest the composition of induced products was dependent on the method of stimulation.

Identification of QTLs controlling somatic embryogenesis using RI population of cultivar ${\times}$ weedy soybean

  • Choi, Pilson;Mano, Yoshiro;Ishikawa, Atsuko;Odashima, Masashi;Umezawa, Taishi;Fujimura, Tatsuhito;Takahata, Yoshihito;Komatsuda, Takao
    • Plant Biotechnology Reports
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    • v.4 no.1
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    • pp.23-27
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    • 2010
  • Quantitative trait loci (QTLs) controlling ability of somatic embryogenesis were identified in soybean. A frame map with 204-point markers was developed using an RI population consisting of 117 $F_{11}$ lines derived from a cross between cultivar 'Keburi' and a weedy soybean 'Masshokutou Kou 502'. The parents differed greatly in their abilities of somatic embryogenesis using immature cotyledons as explants. The ability of somatic embryogenesis was evaluated in five different experiments: the $F_{11}$ (evaluated in 1998) and $F_{15}$ (2002) generations cultured on basal media supplemented with $40\;mg\;l^{-1}$ 2,4-D (2,4-D1998 and 2,4-D2002), $F_{14}$ (2001) generation on medium with $40\;mg\;l^{-1}$ 2,4-D and high sucrose concentration [2,4-D2001 ($30\;g\;l^{-1}$ sucrose)], and the $F_{11}$ (1998) and $F_{12}$ (1999) generations on medium with $10\;mg\;l^{-1}$ NAA (NAA1998 and NAA1999). The RILs showed wide and continuous variations in each of the five experiments. In the composite interval mapping analysis, 2 QTLs were found in group 8 (D1b + W, LOD = 5.42, $r^2$ = 37.5) in the experiment of 2,4-D1998 and in group 6 (C2, LOD = 6.03, $r^2$ = 26.0) in the experiment of 2,4-D2001 (high concentration sucrose). In both QTLs, alleles of 'Masshokutou Kou 502' with high ability of somatic embryogenesis contributed to the QTLs. For the other three experiments, no QTL was detected in the criteria of LOD >3.0, suggesting the presence of minor genes.