• 제목/요약/키워드: Agriculture Technology

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Structure Analysis of the Full Length PDI Genomic DNA Isolated from Bombyx mori

  • Kim, Sung-Wan;Goo, Tae-Won;Yun, Eun-Young;Park, Kwang-Ho;Hwang, Jae-Sam;Kang, Seok-Woo;Kwon, O-Yu
    • 한국잠사학회:학술대회논문집
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    • 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
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    • pp.44-44
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    • 2003
  • Protein disulfide isomerase (PDI) is not only an isomerase catalyzing the formation of native disulfide bond(s) of nascent peptide, but also a molecular chaperone assisting chain folding. We have already reported the structure of a cDNA (bPDl) encoding PDI from Bombyx mori and the function of PDI as foldase in assisting protein folding. (omitted)

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Visualization of Artificially Deposited Submicron-sized Aerosol Particles on the Surfaces of Leaves and Needles in Trees

  • Yamane, Kenichi;Nakaba, Satoshi;Yamaguchi, Masahiro;Kuroda, Katsushi;Sano, Yuzou;Lenggoro, I. Wuled;Izuta, Takeshi;Funada, Ryo
    • Asian Journal of Atmospheric Environment
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    • 제6권4호
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    • pp.275-280
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    • 2012
  • To understand the effect of aerosols on the growth and physiological conditions of trees in forests, it is important to know the state of aerosols that are deposited on the surface of the leaves or needles. In this study, we developed methods of visualization of submicron-sized aerosols that were artificially deposited from the gas-phase or liquid phase onto tree leaves or needles in trees. Firstly, we used field-emission scanning electron microscopy (FE-SEM) to observe black carbon (BC) particles that were artificially sprayed onto the leaves or needles. The distribution of BC particles deposited on the leaves and needles were distinguished based on the size and morphological features of the particles. The distribution and agglomerates size of BC particles differed between two spraying methods of BC particles employed. Secondly, we tried to visualize gold (Au) particles that were artificially sprayed onto the leaves using energy dispersive X-ray spectrometry (EDX) coupled to FE-SEM. We detected the Au particles based on the characteristic X-ray spectrum, which was secondarily generated from the Au particles. In contrast to the case of BC particles, the Au particles did not form agglomerates and were uniformly distributed on the leaf surfaces. The present results show that our methods provide useful information of adsorption and/or behavior of fine particles at the submicron level on the surface of the leaves.

Effects of Long-term Exposure to Black Carbon Particles on Growth and Gas Exchange Rates of Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica Seedlings

  • Yamaguchi, Masahiro;Otani, Yoko;Takeda, Kenta;Lenggoro, I. Wuled;Ishida, Atsushi;Yazaki, Kenichi;Noguchi, Kyotaro;Sase, Hiroyuki;Murao, Naoto;Nakaba, Satoshi;Yamane, Kenichi;Kuroda, Katsushi;Sano, Yuzou;Funada, Ryo;Izuta, Takeshi
    • Asian Journal of Atmospheric Environment
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    • 제6권4호
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    • pp.259-267
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    • 2012
  • To clarify the effects of black carbon (BC) particles on growth and gas exchange rates of Asian forest tree species, the seedlings of Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica were exposed to BC particles with sub-micron size for two growing seasons from 1 June 2009 to 11 November 2010. The BC particles deposited after the exposure to BC were observed on the foliar surface of the 4 tree species. At the end of the experiment, the amount of BC accumulated on the foliar surface after the exposure to BC aerosols were 0.13, 0.69, 0.32 and 0.58 mg C $m^{-2}$ total leaf area in F. crenata, C. sieboldii, L. kaempferi and C. japonica seedlings, respectively. In August 2010, the exposure to BC particles did not significantly affect net photosynthetic rate under any light intensity, stomatal diffusive conductance to water vapour ($g_s$), stomatal limitation of photosynthesis, response of $g_s$ to increase in vapour pressure deficit and leaf temperature under light saturated condition in the leaves or needles of the seedlings. These results suggest that the BC particles deposited on the foliar surface did not reduce net photosynthesis by shading, did not increase leaf temperature by absorption of irradiation light, and did not induce plugging of stomata in the leaves or needles of the seedlings. There were no significant effects of BC particles on the increments of plant height and stem base diameter during the experimental period and the whole-plant dry mass at the end of the experiment. These results indicate that the exposure to BC particles with sub-micron size for two growing seasons did not significantly affect the growth and leaf or needle gas exchange rates of F. crenata, C. sieboldii, L. kaempferi and C. japonica seedlings.

Initiative of the Asian Network for Sustainable Organic Farming Technology

  • Lee, Y.;Choi, H.S.;Lee, S.M.;Lee, H.J.
    • 한국유기농업학회지
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    • 제19권spc호
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    • pp.111-114
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    • 2011
  • Construction of the Asian Network for Sustainable Organic Farming Technology (ANSOFT) will be cooperatively administered by the public researchers in 12 Asian member countries from 2010 to 2012. ANSOFT will bring forward multiple reports, which will be constantly renewed by the member countries, regarding environmental issues, plant and landscape protection techniques, regulations and policies of each country's government on an organic agriculture, and natural resources such as organic seeds and biological agents.

Assay of the Bombyx mori Recombinant Protein Disulfide Isomerase (bPDI) Acivity

  • Goo, Tae-Won;Yun, Eun-Young;Kim, Sung-Wan;Park, Kang-Ho;Hwang, Jae-Sam;Kang, Seok-Woo;Park, Soo-Jung;Kwon, O-Yu
    • 한국잠사학회:학술대회논문집
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    • 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
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    • pp.45-45
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    • 2003
  • Protein disulfide isomerase (PDO) is an essential protein which is localized to the endoplasmic reticulum (ER) of eukaryotic cells. It catalyses the formation and isomerization of disulfide bonds during the folding of secretory proteins. We have isolarted a cDNA encoding PDI from Bombyx mori (bPDI), in which an open reading frame of 494 mino acid (55.6kDa) is shown. (omitted)

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