• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
• /
• 2003.04a
• /
• pp.22-39
• /
• 2003

Biological activity of phenolic compounds in seeds and leaves of safflower (Carthamu tinctorius L.) were evaluated using several in vitro and in vivo assays. Six phenolic constituents were isolated from the seeds and identified as N-feruloylserotonia, N- (p-coumaroyl)serotonin, matairesinol, 8′-hydroxyarctigenin, acacetin 7-O-$\beta$-D-glucoside (tilianine) and acacetin. Six phenolic compounds exhibited considerable antioxidative activity, and especially two serotonins showed potent DPPH radical scavenging activity and antiperoxidative activity against rat liver microsomal lipid peroxidation induced by the hydroxyl radical generated via a Fenton-type reaction. Additionally, six phenolic compounds possessed comparable cytotoxicity against three cancer cells, Hela cell, MCF-7 and HepG2 cell, and particularly acacetin and its glycosides had the most potent cytotoxicity. Moreover, we found that feeding safflower seeds attenuated bone loss, and lowered levels of plasma and liver lipids in ovariectomized rats. Serotonins, lignans and flavones stimulated proliferation of the osteoblast-like cells in a dose-dependent manner (10$^{-15}$ ~10$^{-6}$ M), as potently as E$_2$ (17$\beta$-estradiol). Particularly, serotonins were mainly responsible for bone-protecting and lipid lowering effects in ovariectomized rats. Meanwhile, eight flavonoids, including a novel quercetin-7-O-(6"-O-acetyl)-$\beta$-D-glucopyranoside and seven kown flavonoids, luteolin quercetin, luteolin 7-O-$\beta$-D-glucopyranoside, luteolin-7-O-(6"-O-acetyl)-$\beta$-D-gluco-pyranoside, quercetin 7-O- -glucopyranoside, acacetin 7-O-$\beta$-D-glucuronide and apigenin-6-C-$\beta$-D-glucopyranosyl-8-C-$\beta$-D-glucopyranoside were first isolated and identified from safflower leaf. Among these flavonoids, luteolin-acetyl-glucoside and $\beta$quercetin- acetyl-glucoside showed potent antioxidative activities against 2-deoxyribose degradation and lipid peroxidation in rat liver microsomes. Luteolin, quercetin and their corresponding glycosides also exhibited strong antioxidative activity, while acacetin glucuronide and apigenin-6, 8-di-C-glucoside were relatively less active. Finally, changes in phenolic compositions were also determined by HPLC in the safflower seed and leaf during growth stages and roasting process to produce standardized supplement powerds. These results suggest that phenolic compounds in the roasted safflower seed and leaf may be useful as potential sources of therapeutic agents against several pathological disorders such as carcinogenesis, atherosclerosis and osteoporosis.

• Journal of the East Asian Society of Dietary Life
• /
• v.19 no.2
• /
• pp.187-194
• /
• 2009

The aim of this study was to investigate the physicochemical characteristics of native Korean Allium wakegi Araki samples, which were grown from seven local seed bulbs(Yesan, Muan, Anmyon-do, Deokjeok-do, Jeju-do, Yecheon, and China) to produce high quality native Korean Allium wakegi Araki. For the proximate composition of samples, moisture contents were in the range of 90.69~92.43%. The crude protein content of the Jeju-do sample was highest compared to the other samples. However, there were no significant differences in total sugar contents between samples. The seed bulb origin did not affect the hardness of the stem part, but was high for the leaves of the Yesan sample compared to the other samples. The results for anti-oxidative activity were as follows: Yesan(2.30 mg/mL) > China(2.51 mg/mL) > Muan (2.56 mg/mL) > Yecheon(2.74 mg/mL) > Jeju-do(2.85 mg/mL) > Anmyon-do(2.87 mg/mL) > Deokjeok-do(3.18 mg/mL). In terms of mineral and amino acid contents, the Yesan sample showed the highest levels, respectively, compared with the other samples. Food values such as contents of total phenolics and pyruvic acid were highest in the Jeju-do sample. These results show that the physicochemical characteristics of Allium wakegi Araki were significantly different according to different seed bulb origins.

• Korean Journal of Microbiology
• /
• v.53 no.3
• /
• pp.208-215
• /
• 2017

A cyclomaltodextrinase (SPCD) gene was cloned from Streptococcus pyogenes ATCC 700294. Its open reading frame consists of 567 amino acids (66.8 kDa), which shows less than 37% of amino acid sequence identity with the other CDase-family enzymes. The homo-dimeric SPCD with C-terminal six-histidines was expressed and purified from Escherichia coli. It showed the highest activity at pH 7.5 and $45^{\circ}C$, respectively. SPCD has the broad substrate specificities against ${\beta}$-cyclodextrin, starch, and maltotriose to produce mainly maltose, whereas it hydrolyzes pullulan to panose. It can also catalyze the hydrolysis of acarbose to glucose and acarviosine-glucose. Interestingly, it showed much higher activity on ${\beta}$-cyclodextrin and acarbose than that on starch, pullulan, or maltotriose, which makes SPCD distinguished from common CDase-family enzymes. Although SPCD has significantly high acarbose-hydrolyzing activity, it showed negligible transglycosylation activity.

• Journal of Bio-Environment Control
• /
• v.26 no.4
• /
• pp.297-300
• /
• 2017

Plant factory can control artificially the environments for crop cultivation, so they can produce high quality agricultural products all year round. This study was carried to select suitable kohlrabi cultivar for hydroponics in a closed-type plant factory system. We used three cultivars of red kohlrabi, 'Asac kohl', 'Kolibri', and 'Purple king' as plant materials. The artificial light source was LED light, light intensity and photoperiod were $249{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and 12/12 hours (day/night period), respectively. Hydroponic cultivation type was used circulating deep flow technique. At 43 days after transplanting, fresh weight of whole plant and tuber and leaf area were not significantly different among cultivars. Shoot dry weight and tuber dry weight were highest in 'Asac kohl' cultivar, and number of leaves was highest in 'Purple king' cultivar. Sugar content and yield were highest in 'Asac kohl' cultivar. Considering the growth and marketable yields, 'Asac kohl' was the optimal kohlrabi cultivar for hydroponic cultivation in a closed-type plant factory system.

• Journal of Mushroom
• /
• v.10 no.1
• /
• pp.3-8
• /
• 2012

In this study, to produce Flammulina velutipes mushroom liquid spawn efficiently and effectively the effects of explosive aeration (supplying air with tiny bubbles) of the liquid culture medium on carbon dioxide concentration and residual sugar content in the medium on carbon dioxide concentration and residual sugar contentin the medium were measured. Carbon dioxide concentrations were measured at the outlet of the incubator. On the third day the explosive aeration greatly increased mycelial growth of the liquid spawn, and carbon dioxide concentration also greatly increased but decreased after 5 days. Free sugar contents in the liquid culture consistantly decreased up to 7 days and thereafter was not detected. The weight of the mycelia were maintained similar levels after 3 days. Total nitrogen content in the liquid medium constantly decreased during the 11days of explosive aeration. The content of free sugars in 7 days of culture was the lowest level, thus the inoculum incubated for 6~7 days was thought to be the most effective. Carbon dioxide concentration measurement at the outlet of the container during the liquid spawn incubation required low cost but was efficient to estimate the degree of mycelial growth to be used as a simple indicator.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.12
• /
• pp.1763-1769
• /
• 2003

Two experiments were conducted to examine the production and quality of cassava hay and its utilization in diets for dairy cows. In experiment I, a $2{\times}2$ Factorial arrangement in a randomized complete block design with 4 replications was carried out to determine the effects of different initial (IC) and subsequent cutting (SC) on yield and composition of cassava plant. The results revealed that cassava could produce from 4 to 7 tonne of DM and 1.2 to 1.6 tonne of CP for the first six months after planting. CP content in cassava plant ranged from 20.8 to 28.5% and was affected by different SC regimes. Condensed tannin in cassava foliage ranged from 4.9 to 5.5%. Initial cutting at 2 months with subsequent cutting at 2 month intervals was the optimal to obtain high dry matter and protein yield. In the second experiment, five crossbred Holstein-Friesian cows in mid lactation with an initial live-weight of 505${\pm}6.1kg$ and average milk yield of 10.78${\pm}1.2kg/d$ were randomly assigned in a $5{\times}5$ Latin square design to study the effects of 2 levels of CH (1 and 2 kg/hd/d) and concentrate (1 to 2 kg of milk and 1 to 3 kg of milk) on milk yield and milk composition. The results showed that cassava hay increased rumen $NH_3-N$ and milk urea nitrogen (MUN) (p<0.05). Cassava hay tended to increase milk production and 4% FCM. Milk protein increased in cows fed cassava hay (p<0.05). Moreover, cassava hay could reduce concentrate levels in dairy rations thus resulting in increased economic returns. Cassava hay can be a good source of forage to reduce concentrate supplementation and improve milk quality.

• Journal of agricultural medicine and community health
• /
• v.16 no.1
• /
• pp.61-69
• /
• 1991

Serodiagnosis of Clonorchis sinensis infections will probably be a first choice tool for screening of clonorchiasis in a future because of increasing difficulties in collection and examination of stools. The sensitive test such as ELISA can he used effectively. However there are some limitations in serological diagnosis for the detection of serum antibody. One of the major problems is the non-specificity of the antigens which produce cross reaction with other helminthic infection sera. To solve this problem. many investigators have tried to purify the antigens used. In this study, we determined the antigenic profile of the crude saline extract antigen of C. sinensis at early developmental stage based on SDS-PAGE and immunoblotting techniques for the purpose of understanding the nature of C. sinensis worm antigen The following results were obtained : 1) The SDS-PAGE showed many protein hands ranging from 10Kd to 91Kd relative molecular weight. Among them, 66, 46, 40, 33, 27, 24, 16, 14 and 10Kd bands were observed as a principle bands. The protein components of C. sinensis changed chronologically during their early developmental period. 44Kd band was stained unclearly in antigen of 2 weeks worm, but changed to concentrated state in antigen of 5 weeks worm. 35Kd band was found in antigen of 2 weeks worm, however this band was disappeared in antigen of 5 weeks worm. 22Kd band also lost its staining property gradually. 2) In spite of differences in antigenic profile, there was no differences in the data obtained by microplate ELISA using each antigen preparation. Absorbance value began to rise in between 2 to 3 weeks after infection. 3) By EITB. serum antibody recognized major protein bands with molecular weight of 91, 85, 63, 46, 40, 33, 24, 14 and 10Kd hand respectively. Among them 66, 33, 17 and 14Kd bands were observed as non-specific band because they reacted even in normal control sera. Generally, gradual increase of positive reactions were observed as the infection period of C. sinensis was prolonged. In other hand, the reaction of 10Kd hand did not occurred when 26th week sera was tested. 4) The positive reactions using antigens of 2 weeks worm, especially on 40 and 24Kd bands, were most strong and sharply demarcated compared to those of 3~5 weeks worm antigen.

• Journal of Environmental Policy
• /
• v.7 no.3
• /
• pp.41-61
• /
• 2008

Although the size of the organic food market in Korea has increased significantly, its further development is hampered by the information asymmetry between the producers and consumers of organic food. It isn't just about revitalizing the market; it's also about Korean farmers surviving an era of trade liberalization. In order to produce more value-added products, the information asymmetry issue has to be resolved regarding the organic food market and other agricultural credence goods such as Han-woo(Korean beef). Therefore, measuring information asymmetry has become a central issue. One way to measure asymmetry is to use Game Theory. However, in practice, estimating payoffs at the industry level is hard to accomplish, and even when it is possible, the reliability of the estimated payoffs is not guaranteed. As an alternative, the concept of Information Entropy(disorder level of information), developed by Shannon(1948), was used in this study. It is proposed that this measure should be used when assessing the level of information asymmetry in the Korean organic food market. Using recent data, it was found that information entropy in the Korean organic food market has been decreasing constantly since 2003. Therefore, it was proposed that measures should be adopted by the government to improve the certification system of organic food.

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.5
• /
• pp.683-689
• /
• 2017

Objective: The experiments were carried out to measure the effect of multivitamin, acidifier and Azolla on growth performance, profitability and lipid profiles of blood of broiler chickens to produce safe and cost effective broilers. Methods: In experiment 1, 240 day-old Cobb-500 broiler chicks were fed diets; $D_1$ (control), $D_2$ ($D_1$ with 1 mL multivitamin/liter water), $D_3$ ($D_1$ with 1 mL acidifier/liter water), $D_4$ ($D_1$ with 1 mL multivitamin and 2 mL acidifier/liter water) having 3 replications in each, and 20 chicks/replication. In experiment 2, 150 day-old Cobb-500 broiler chicks were fed diets; $T_1$ (control), $T_2$ (5% Azolla in the diet), $T_3$ (7% Azolla in the diet) and $T_4$ ($T_1$ with 1 mL multivitamin and 1 mL acidifier/liter water) having 3 replications in each, and 20 chicks/replication in control, and 10 chicks/replication in the remaining dietary treatment groups for 35 days. Results: In experiment 1, the highest live weight was observed in $D_4$ (p<0.05), however, feed intake was statistically similar between diets (p>0.05). The lowest feed conversion ratio (FCR) (p<0.001) and mortality (p<0.05) were observed in $D_2$ followed by $D_4$, $D_1$, and $D_3$, respectively. There were no significant differences between diets for feed cost and net profit (p>0.05). However, evidently but not significantly, the highest net profit was obtained in $D_2$ followed by $D_4$, $D_1$, and $D_3$, respectively. In experiment 2, the highest live weight (p<0.05) and feed intake (p<0.001) were observed in $T_4$. Mortality (p<0.01), FCR (p<0.01), feed cost (p<0.05) and net profit (p<0.05) were significantly different among diets. Considering net profit, $T_2$ was the best performing dietary group followed by $T_3$, $T_1$, and $T_4$, respectively. The lowest lipid profiles were observed in $D_3$ followed by $D_1$, $D_4$, and $D_2$, respectively (p<0.05). In experiment 2, the lowest total cholesterol, TG, and the highest amount of high density lipoprotein were observed in $T_2$, followed by $T_3$, $T_1$, and $T_4$, respectively (p<0.05). Evidently but not significantly, low density lipoprotein was the highest in $T_2$ followed by $T_3$, $T_4$, and $T_1$, respectively (p>0.05). Conclusion: In conclusion, Azolla and acidifier reduced lipid profiles of broiler chickens. Considering net profit and lipid profiles, 5% Azolla may be the suitable dietary group for producing safe and profitable broilers. However, more studies are needed to confirm this study prior to suggesting using Azolla in the poultry industry.

• Nutritional Sciences
• /
• v.9 no.2
• /
• pp.92-96
• /
• 2006

Studies were carried out to determine the effect of feeding diet containing 5% canola oil on growth, feed efficiency, and fatty acid profile of bacon in finishing pigs and of longissimus muscle in horses fattening for meat production. In experiment 1, twenty cross-bred barrows and twenty cross-bred gilts (average weight, 80 kg) were blocked by sex and weight, and five barrows or five gilts were allotted to one of eight pens $(6.25m^2/pen)$, respectively. Four pens (two with barrows and two with gilts) randomly selected were assigned to a control diet containing 5% tallow and the remaining four pens to a diet containing 5% canola oil. The average daily weight gain, daily feed intake and feed efficiency over a 6-wk feeding period were not different (p>0.05) between the two diets, nor was backfat thickness. Fatty acid profile in bacon fat showed that the 0-3 fatty acid ($\alpha-linolenic$ acid) content in pigs fed diet containing 5% canola oil was approximately three times (P<0.01) as much as in pigs fed tallow. In experiment 2, thirty-two Jeju horses (average $weight{\pm}SE,\;244{\pm}5kg$) were blocked by sex and weight, and two horses of the same sex and similar body weight were allotted to one $(15m^2/pen)$ of eight pens. Eight pens (four with males and four with females) selected randomly were assigned to a control diet containing 5% tallow and the remaining eight pens to a diet containing 5% canola oil. The average daily weight gain, daily feed intake and feed efficiency for concentrates without roughages over a 5-month feeding period were not different (P>0.05) between the two diet groups. Fatty acid profile in the muscle fat showed that the 0-3 fatty acid (a-linolenic acid) content in horses fed diet containing 5% canola oil was approximately two times (P<0.01) that in horses fed tallow. The increased (P<0.01) 0-3 fatty acid content in pigs and horses fed canola oil decreased the ratio of n-6 to n-3 fatty acids compared to the control, indicating a significant improvement in pork and horsemeat fatty acid profile for health benefit. Our study demonstrated that feeding diet containing 5% canola oil may help produce pork and horsemeat with more health benefit, increasing their $\alpha-linolenic$ acid content without deleterious effects on growth of pigs and horses.