• Title/Summary/Keyword: Agricultural activities

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In vitro and In vivo Activities of a Biocontrol Agent, Serratia plymuthica A2l-4, Against Phytophthora capsici

  • Shen, Shun-Shan;Park, Ok-Hee;Lee, Sun-Mi;Park, Chang-Seuk
    • The Plant Pathology Journal
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    • v.18 no.4
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    • pp.221-224
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    • 2002
  • In vitro and in vivo activities of a biocontrol agent, Serratia plymuthica strain A2l-4, was evaluated for the control of Phytophthora blight of pepper, Strain A2l-4 inhibited mycelial growth, germination of zoosporangia and cystospores, and formation of zoospore and zoosporangia of Phytophthora capsici in vitro. In the pot experiment, incidence of Phytophthora blight of pepper in non-treated control was 100% at 14 days after inoculation, while no disease was observed in the plot treated with S. plymuthica A2l-4. In the greenhouse test, infection rate of pepper in the non-treated plots was 74.5%, while it was only 12.6% in the plots treated with A2l-4. Results indicate that S. plymuthica A2l-4 is a potential biocontrol agent for Phytophthora blight of pepper.

Chemical Characteristics and Physiological Activities of Plums (Oishiwase and Formosa) (자두의 화학적 특성 및 생리활성)

  • Jung, Gi-Tai;Ju, In-Ok;Choi, Dong-Geun;Jeong, Jong-Sung;Ryu, Jeong;Ko, Bok-Rai;Choi, Joung-Sik
    • Korean Journal of Food Science and Technology
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    • v.37 no.5
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    • pp.816-821
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    • 2005
  • Chemical characteristics and physiological activities of plums (Oishiwase and Formosa) were evaluated. Proximate composition of plums consisted of (w/w) 1.9-6.2% protein, 2.3-7.1% fat, 3.5-4.1% ash, and 84,1-88,7% carbohydrate. Organic acids, such as oxalic, malic, succinic, and acetic acids were detected, except in Oishiwse acetic acid was not detected. Free sugars consisted of sorbitol, glucose, fructose and sucrose. Total fiber and total phenolics compounds of plum rind were higher than those of flesh. Electron-donating abilities of rind ethanol extracts were higher than those of BHA and tocopherol, and were twofold higher than those of flesh extracts. Nitrite-scavenging abilities of rind and flesh extracts were significantly higher (over 97%) at pH 1.2 and 3.0. Tyrosinase-inhibitory activities ranged 88.5 to 100%. SOD-like activities of all extracts were weak at 22.7 to 27.2%.

Heterologous Expression of Rhizopus Oryzae CYP509C12 Gene in Rhizopus Nigricans Enhances Reactive Oxygen Species Production and 11α-Hydroxylation Rate of 16α, 17-Epoxyprogesterone

  • Shen, Chaohui;Gao, Xiyang;Li, Tao;Zhang, Jun;Gao, Yuqian;Qiu, Liyou;Zhang, Guang
    • Mycobiology
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    • v.47 no.3
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    • pp.301-307
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    • 2019
  • The $11{\alpha}$-hydroxylation of $16{\alpha}$, 17-epoxyprogesterone (EP) catalyzed by Rhizopus nigricans is crucial for the steroid industry. However, lower conversion rate of the biohydroxylation restricts its potential industrial application. The $11{\alpha}$-steroid hydroxylase CYP509C12 from R. oryzae were reported to play a crucial role in the $11{\alpha}$-hydroxylation in recombinant fission yeast. In the present study, the CYP509C12 of R. oryzae (RoCYP) was introduced into R. nigricans using the liposome-mediated mycelial transformation. Heterologous expression of RoCYP resulted in increased fungal growth and improved intracellular reactive oxygen species content in R. nigricans. The $H_2O_2$ levels in RoCYP transformants were approximately 2-folder that of the R. nigricans wild type (RnWT) strain, with the superoxide dismutase activities increased approximately 45% and catalase activities decreased approximately 68%. Furthermore, the $11{\alpha}$-hydroxylation rates of EP in RoCYP transformants (C4, C6 and C9) were 39.7%, 38.3% and 38.7%, which were 12.1%, 8.2% and 9.4% higher than the rate of the RnWT strain, respectively. This paper investigated the effect of heterologous expression of RoCYP in R. nigricans, providing an effective genetic method to construct the engineered strains for steroid industry.

A comparative study on the phytochemical and anti-oxidant activity differences in HongJam prepared with various silkworm varieties

  • Nguyen, Phuong;Kim, Seong-Wan;Jo, You-Young;Beteta, Sofia Pallais;Kang, Sang Kook;Kim, Soo Bae;Kim, NamSook;Kweon, Hae Yong;Kim, Kee-Young;Koh, Young Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.41 no.2
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    • pp.19-27
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    • 2020
  • The aim of this study was to elucidate the difference in the amount of phytochemicals and anti-oxidant activities of the freeze-dried 5th instar 3rd day silkworm powder (FDSP) and 7 HongJams (HJs). Three primary colors of light of 8 silkworm powders revealed why each sample had unique color. The visual and absorption spectrum analyses of 80% methanol (MeOH) extracts of 8 silkworm powders revealed that white jade (WJ)-FDSP extracts showing strong forest-green colors had different absorption spectrum from 7 HJs. Among 7 HJs, the absorption spectrum of light green silkworm (LGS)-HJ extract was different from those of other HJ extracts. The amounts of total flavonoids and phenolic compounds of WJ-FDSP were 423.0±9.21% and 252.3±12.86%, respectively, more than those of WJ-HJ. Among 7 HJs, the amounts of total flavonoids and phenolic compounds in LGS-and red silkorm (RS)-HJ extracts were significantly more than those of WJ-HJ extract. Consistent with the amounts of phytochemicals, radical scavenging activity and reducing ability of WJ-FDSP extract were significantly higher that other HJ extracts. Among 7 silkworm powder extracts, LGS-HJ extract showed significantly higher radical scavenging activity and reducing ability than those of WJ-HJ extract. But, RS-HJ extract only showed significantly higher radical scavenging activity than that of WJ-HJ extract. Taken together, the amounts of phytochemicals in 8 silkworm powders contributed on their colors and anti-oxidant activities.

Enhancing Activities of the Korean Rural Leaders' Association for Fostering the Voluntary Leadership in Rural Korea (농촌의 자원지도력 육성을 위한 농업인 조직의 활력화 방안 연구: 농촌지도자회 사례)

  • Kim, Jin-Goon;Kang, Jung-Hyun;Kim, In-Kyu
    • Journal of Agricultural Extension & Community Development
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    • v.15 no.2
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    • pp.325-338
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    • 2008
  • The objective of this study was to develop the strategies for enhancing activities of the "Korean Rural Leaders' Association" to foster the voluntary leadership in rural area. Literature review and questionnaire survey were carried out. The results of the study were as follows: Firstly, for enhancing the organizational competency, it was regarded as the important components that developing an organizational vision, improvement of structure and functions, promotion of the organizational management efficiency including human resource, securing new members and establishment of organizational culture. And secondly, for accelerating the organizational activities, it was expected to give more concern to such activities as public services, spokesman's role for the farmers, organizational / international cooperations and research at central level of the association.

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Analysis and Classification on CSR activities of global companies in rural area (농촌으로 확대되는 글로벌 기업의 CSR 활동 유형 분석)

  • Shin, Hyeon Am;Lee, Won Jun
    • Journal of Korean Society for Quality Management
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    • v.43 no.3
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    • pp.439-452
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    • 2015
  • Purpose: The purpose of this study is to propose useful suggestions by analyzing cases as to how global companies have implemented a rural outreach as a part of their CSR activities. Methods: Case studies were conducted. The cases were selected through the literature review and, based on two-by-two matrices, four types of cases were classified. Results: The results of this study are as follows; CSR activities of global companies in the rural area were categorized into two parts - "where to support(agricultural business or rural area)" and "how to support(whether to connect with business or not)." Conclusion: Domestic companies need to refer to the result of this study to increase the diversity and the effectiveness of their rural outreaches of CSR activities.