• Journal of Microbiology and Biotechnology
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• 제24권2호
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• pp.245-253
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• 2014

A fibrinolytic enzyme was produced by an edible mushroom of Pleurotus ostreatus using submerged culture fermentation. The enzyme was purified from the culture supernatant by applying a combination of freeze-thaw treatment, ammonium sulfate precipitation, hydrophobic interaction, and gel filtration chromatographies. The enzyme was purified by a 147-fold, with a yield of 7.54%. The molecular masses of the enzyme an determined by gel filtration and SDS-PAGE were 13.6 and 18.2 kDa, respectively. The isoelectric point of the enzyme was 8.52. It hydrolyzed fibrinogen by cleaving the ${\alpha}$ and ${\beta}$ chains of fibrinogen followed by the ${\gamma}$ chains, and also activated plasminogen into plasmin. The enzyme was optimally active at $45^{\circ}C$ and pH 7.4. The enzyme activity was completely inhibited by EDTA, whereas protease inhibitors of TPCK, SBTI, PMSF, aprotinin and pepstatin showed no inhibition on its activity. The partial amino acid sequences of the enzyme as determined by Q-TOF2 were ATFVGCSATR, GGTLIHESSHFTR, and YTTWFGTFVTSR. These sequences showed a high degree of homology with those of metallo-endopeptidases from P. ostreatus and Armillaria mellea. The purified enzyme can also be applied as a natural agent for oral fibrinolytic therapy or prevention of thrombosis.

• 한국포장학회지
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• 제20권1호
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• pp.7-15
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• 2014

Agar-based composite films were prepared with variety of food processing and agricultural processing waste materials in order to screen natural lingo-cellulosic resources for the value-added utilization of the under-utilized materials. The effect of these waste materials (10 wt% based on agar) on mechanical properties, moisture content (MC), water vapor permeability (WVP), water absorption behavior of biocomposite films were investigated. Biocomposite films prepared with various fibers resulted in significant increase or decrease in color and percent transmittance. The MC, WVP, and surface hydrophobicity of biocomposite films increased significantly by incorporation of fibers, while the water uptake ratio and solubility of the film decreased. SEM images of biocomposite film showed better adhesion between the fiber and agar polymer. Among the tested cellulosic waste materials, rice wine waste, onion and garlic fibers were promising for the value-added utilization as a reinforcing material for the preparation of biocomposite food packaging films.

• 식품과학과 산업
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• 제49권2호
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• pp.51-55
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• 2016

The needs to develop high value-added foods are expanding due to the trends of food market, such as importance of food security by enlarged international free trades and expanded market size for processed foods. However, our home country exhibits limit transport to development of new products and high-value added area compared to other advanced countries. For this reason Ministry of Agriculture, Food and Rural Affairs and Institute of Planning and Evaluation for Technology have launched "High Value-Added Food Technology Development Program" for improvement of value of food resources for practical use since 2013. The program contains research to increase the values of agricultural and animal resources and the construction of database. Main research topics are studies in pretreatment techniques to improve the values of agricultural and animal resources, improvement of acceptability, and processing qualities that would be engrafted on materializaton and commercialization technologies. In addition the construction of a consolidated database regarding the research achievements is included in the program. Currently, studies in processing qualities for about 30 food materials re under progress, being suitable for materialization, for examples drying, fermentation, grinding, heat-treatment, and so on. The research results are provided in public through the consolidated database website after reconstruction in the form of united database format. To date the database containing the about 300 of research contents for process qualities has been constructed.

• Animal Bioscience
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• 제34권9호
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• pp.1532-1543
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• 2021

Objective: This study was aimed to establish a quantitative detection method for meat contamination based on specific polypeptides. Methods: Thermally stable peptides with good responses were screened by high resolution liquid chromatography tandem mass spectrometry. Standard curves of specific polypeptide were established by triple quadrupole mass spectrometry. Finally, the adulteration of commercial samples was detected according to the standard curve. Results: Fifteen thermally stable peptides with good responses were screened. The selected specific peptides can be detected stably in raw meat and deep processed meat with the detection limit up to 1% and have a good linear relationship with the corresponding muscle composition. Conclusion: This method can be effectively used for quantitative analysis of commercial samples.

• 한국식품영양학회지
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• 제23권3호
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• pp.299-305
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• 2010

주박 추출물을 열수와 에탄올로 추출하여 항균활성, 항산화, DPPH 라디컬 소거능 그리고 SOD 유사활성을 실험하였다. 주박의 추출물은 B. subtilis, Staph. aureus, P. aeruginosa 그리고 E. coli 균주에 대해서 모든 항균활성을 가지고 있었다. 대두유 유화물을 이용한 주박의 항산화력은 control보다 추출물이 더 높았다. 그리고 주박 추출물의 농도가 200 ppm일때 효과가 있었다. 주박 추출물의 DPPH의 소거능은 80% 이상의 저해 효과 나타냈다. SOD 유사활성은 에탄올 추출물보다 열수 추출물이 더 높게 나타났다. 주박의 지속적인 연구를 함으로써 화장품 원료로서 가능성이 있고, 또한 주박을 다른 용매 추출법에 의해 식품의 기능성 원료로 기대할 수 있을 것으로 사료된다.

• 농업과학연구
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• 제40권1호
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• pp.61-78
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• 2013

The following four aspects should be examined in detail to revitalize North-East Asian agricultural cooperation specialized with Korea, Japan, and China. First, quality and safety standards of agricultural food should be unified. Secondly, it is necessary to build a food security cooperative system in North-East Asia. If three countries build the system, they can overcome a problem such as unstable supply and demand due to natural disaster, and also they can cope with food crisis. Thirdly, to establish North-East Asian Agro-valley is needed. After forming food production belt that connects Korea, Hokkaido in Japan, and the three north-eastern provinces in China, it is possible to realize high value-added agriculture with producing and processing of complementary food for partner country. Lastly, trilateral FTA negotiations should be managed carefully with operating the agricultural special zone and exclusion or prolong period of removal of tariff on sensitive items.

• Asian-Australasian Journal of Animal Sciences
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• 제30권6호
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• pp.857-864
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• 2017

Objective: The purpose of this study was to investigate the influence of AMP-activated protein kinase (AMPK) activation on protein acetylation and glycolysis in postmortem muscle to better understand the mechanism by which AMPK regulates postmortem glycolysis and meat quality. Methods: A total of 32 mice were randomly assigned to four groups and intraperitoneally injected with 5-Aminoimidazole-4-carboxamide1-${\beta}$-D-ribofuranoside (AICAR, a specific activator of AMPK), AICAR and histone acetyltransferase inhibitor II, or AICAR, Trichostatin A (TSA, an inhibitor of histone deacetylase I and II) and Nicotinamide (NAM, an inhibitor of the Sirt family deacetylases). After mice were euthanized, the Longissimus dorsi muscle was collected at 0 h, 45 min, and 24 h postmortem. AMPK activity, protein acetylation and glycolysis in postmortem muscle were measured. Results: Activation of AMPK by AICAR significantly increased glycolysis in postmortem muscle. At the same time, it increased the total acetylated proteins in muscle 45 min postmortem. Inhibition of protein acetylation by histone acetyltransferase inhibitors reduced AMPK activation induced increase in the total acetylated proteins and glycolytic rate in muscle early postmortem, while histone deacetylase inhibitors further promoted protein acetylation and glycolysis. Several bands of proteins were detected to be differentially acetylated in muscle with different glycolytic rates. Conclusion: Protein acetylation plays an important regulatory role in postmortem glycolysis. As AMPK mediates the effects of pre-slaughter stress on postmortem glycolysis, protein acetylation is likely a mechanism by which antemortem stress influenced postmortem metabolism and meat quality though the exact mechanism is to be elucidated.

• 한국축산식품학회지
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• 제40권1호
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• pp.1-10
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• 2020

Various processing methods have a great impact on the physiochemical and nutritional properties of meat that are of health concern. Hence, the postmortem processing of meat by different methods is likely to intensify the potential effects on protein oxidation. The influence of meat protein oxidation on the modulation of the systemic redox status and underlying mechanism is well known. However, the effects of processed meat proteins isolated from different sources on gut microbiota, oxidative stress biomarkers, and metabolomic markers associated with metabolic syndromes are of growing interest. The application of advanced methodological approaches based on OMICS, and mass spectrometric technologies has enabled to better understand the molecular basis of the effect of processed meat oxidation on human health and the aging process. Animal studies indicate the involvement of dietary proteins isolated from different sources on health disorders, which emphasizes the impact of processed meat protein on the richness of bacterial taxa such as (Mucispirillum, Oscillibacter), accompanied by increased expression of lipogenic genes. This review explores the most recent evidences on meat processing techniques, meat protein oxidation, underlying mechanisms, and their potential effects on nutritional value, gut microbiota composition and possible implications on human health.

• 동아시아식생활학회지
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• 제20권6호
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• pp.896-902
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• 2010

오미자는 유기산 함량이 5~7%로써 대단히 높기 때문에 기호성이 우수한 과일주를 제조하려면 희석이 불가피하다. 본 연구에서는 오미자를 희석하는데 향기와 총산 함량이 적고 당분이 풍부한 배를 이용하였는데, 오미자와 배를 1:9~1:15로 희석할 경우, 일반 과일주의 총산 농도인 0.6~0.7%와 비슷하게 되었다. 오미자 배 혼합 발효주의 경우 오미자의 붉은색이 엷어지는데, 이러한 적색을 보정하기 위하여 복분자와 개량머루 발효주를 적용한 결과, 복분자 발효주의 색상이 오미자 색상과 비슷한 것으로 판명되었다. 오미자 배혼합 발효주의 적색도 보정용으로 복분자 발효액을 첨가할 경우, 무게 비율로 3~5% 첨가시 오미자의 붉은색 이미지에 적합한 것으로 나타났다. 오미자 배 혼합 발효주의 쓴맛을 완화시키면서 단맛을 부여하며 효모에 의한 2차 발효를 방지하기 위한 방법으로 비발효성 당인 자일리톨을 첨가한 결과, 오미자 배 혼합 발효주에 대하여 자일리톨을 2~5% 첨가한 것이 기호성이 우수한 것으로 나타났다.

• Journal of Biosystems Engineering
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• 제39권2호
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• pp.142-149
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• 2014

Purpose: A multispectral algorithm for detection and differentiation of defective (defects on apple skin) and normal Red Delicious apples was developed from analysis of a series of hyperspectral line-scan images. Methods: A fast line-scan hyperspectral imaging system mounted on a conventional apple sorting machine was used to capture hyperspectral images of apples moving approximately 4 apples per second on a conveyor belt. The detection algorithm included an apple segmentation method and a threshold function, and was developed using three wavebands at 676 nm, 714 nm and 779 nm. The algorithm was executed on line-by-line image analysis, simulating online real-time line-scan imaging inspection during fruit processing. Results: The rapid multispectral algorithm detected over 95% of defective apples and 91% of normal apples investigated. Conclusions: The multispectral defect detection algorithm can potentially be used in commercial apple processing lines.