• 농업과학연구
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• 제39권1호
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• pp.1-8
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• 2012

Rapid loss of grape berry quality after harvest occurs due to poor postharvest management. Understanding physiological properties between cultivars is required to develop practical technologies to control fast quality deterioration of tables grapes. Physiological characteristics of whole cluster, rachis and berries were examined to find their effects on postharvest behaviour of table grapes. 'Tamnara' showed high respiration rate and browning of rachis compared to 'Campbell Early'. Weight loss of rachis of 'Cheongsoo' was highest of three cultivars. Berry decay in 'Cheongsoo' begins at the connection portion between peduncle and berry, and berry decay of 'Cheongsoo' was the most severe among three cultivars. Precooling at $0^{\circ}C$ showed better results than cooling at $10^{\circ}C$ regardless of cultivars but cooling effect decreased when simulated marketing period increased up to 10 days. Plastic film wrapping was effective on reducing decay in general but not at higher marketing temperature. For successful commercialization of newly developed grapes, the inhibition technology of rachis browning in 'Tamnara' is required and decay control in 'Cheongsoo' through establishment of cold chain system is recommended.

• 한국균학회지
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• 제25권4호통권83호
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• pp.362-368
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• 1997

재조합 DNA probe를 이용하여 국내 Fusarium oxysporum 분화형간의 분류 가능성을 탐색하고 그들의 유전적 변이를 분석하였다. F. oxysporum f. sp. lycopersici, cucumerinum, fragariae, garlic, sesami 등 5종의 분화형을 공시하여 RFLP 분석을 실시하였다. Repetitive copy clone인 세종의 재조합 클론 pFC46, pFC52, pFC57을 이용하여 HindIII로 처리한 F. oxysporum의 genomic DNA에 대해 southern hybridization한 결과 나타난 밴드의 양상은 분화형에 따라 차이가 명확히 밝혀져 이들을 이용한 분류가 가능하였다. 또한 RFLP 분석 결과 f. sp. sesami는 다른 분화형에 비해 다소 변이가 심했으나 다른 분화형들은 변이가 거의 없어 f. sp. sesami를 제외한 f. sp. lycopersici 등 4종의 Fusarium oxysporum 분화형의 균주들은 채집 지역에 관계없이 대체로 유전적으로 안정되어 있는 것으로 판단되었다. Hybridization밴드의 양상에 기초하여 유전적 유연관계를 집괴 분석한 결과 각 분화형별로 유사도가 매우 높게 별도의 유사군을 형성하였다.

• 한국잡초학회지
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• 제8권1호
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• pp.9-14
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• 1988

논 포장(圃場)(전북(全北) 완주군(完州郡) 소양면(所陽面) 죽절리(竹節里))에서 발생(發生)되는 피속(屬) 3변종(變種)과 이와 형태적(形態的)으로 구분(區分)이 되는 5종(種)의 피를 수집(蒐集)하여 이들의 생리생태적(生理生態的) 형질(形質)을 비교(比較) 검토(檢討)하였다. 8종(種)의 수집종(蒐集種)들은 종자(種子) 휴면성(休眠性), 상대생장율(相對生長率), 출수(出穗)에 요(要)하는 일수(日數), 수(穗) 형태(形態) 등에서 변이(變異)를 보였으며 이들 형질(形質)은 중심(中心)으로 한 cluster 분석(分析) 결과(結果) 피와 이의 생태형(生態型) 2종(種), 돌피와 이의 생태형(生態型) 1종(種) 및 물피와 이의 생태형(生態型) 2종(種)으로 구분(區分)지어졌다. 피속(屬) 3 변종(變種)과 이의 생태형(生態型)들은 butachlor에 대하여 발아(發芽) 및 유묘(幼苗) 생장(生長)에 있어서 서로 다른 내성(耐性) 차이(差異)를 보였다.

• 원예과학기술지
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• 제34권1호
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• pp.15-23
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• 2016

A study was conducted to evaluate the effects of irrigation (amount, interval) on tree growth, fruit quality, and cracking of organic 'Campbell Early' grapevine in 2012 and 2013. Three irrigation treatments were applied using a sprinkler system from mid-June to mid-August in 2012 and 2013, as follows: 10 mm was applied daily (10 mm-IR), 20 mm was applied every two days (20 mm-IR), and 30 mm was applied every five days (30 mm-IR). Soil electrical conductivity (EC) and temperature were found to be greatest in the 10 mm-IR treatment in both years. Soil moisture content ranged between 20-40% in the 10 mm-IR, between 20-60% in the 20 mm-IR, and between 20-70% in the 30 mm-IR treatment plots. The total number of leaves per shoot and shoot growth were found to be greatest in the 20 mm-IR and 30 mm-IR treatments, respectively. Cluster and berry weights, and cluster and berry sizes were not consistently affected by the treatments. The 10 mm-IR treatment resulted in an increase in fruit SSC, SSC/acidity ratio, and berry skin pigmentation ($b^*$; blue). Approximately 5% of fruit cracking was observed on average over both years in the 10 mm-IR-treated fruit, while the 30 mm-IR treatment resulted in nearly 18% of cracking in 2012. Average marketable fruit yield per year over two years was greatest for the 10 mm-IR treatment ($24.4t{\cdot}ha^{-1}$) followed by the 30 mm-IR treatment ($22.7t{\cdot}ha^{-1}$) and lastly the 20 mm-IR treatment ($22.2t{\cdot}ha^{-1}$). Thus, the 10 mm-IR treatment represents a suitable irrigation regimen for controlling leaf and shoot growth of vines grown under a rain-shelter system in sandy loam soils, while improving fruit sugar contents and skin color and limiting fruit cracking.

• Environmental Engineering Research
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• 제24권1호
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• pp.45-53
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• 2019

Water and soil properties in paddy fields subjected to organic and conventional farming were characterized over the rice cycle in South Korea. To achieve the goals of this study, we sampled and analyzed soil and water from 24 organic paddy plots and 11 conventional paddy plots in March, May, August, and October 2016. The results were analyzed using statistical analyses, including analysis of variance (ANOVA), cluster analysis, and principal component analysis. The ANOVA results showed that water content (WC), electrical conductivity (EC), organic matter (OM), and available phosphorus ($P_2O_5$) in soil varied significantly (p < 0.01) depending on the farming method. Higher OM, EC, and $P_2O_5$ of soil were observed in the conventional paddies than in the organic paddies. All soil properties, except pH and ammonium, depended on seasonal variation. Cluster analysis revealed that soil properties in May were distinctly separated from those in other seasons mainly due to basal fertilization. The principal component analysis distinguished the soil properties in different seasons, but such a distinction was not observed between the soil properties in organic and conventional paddies. Low contents of WC, OM, and total N were observed in March. High concentrations of nitrate and total P were observed in May, but these were low in August and October. The soils from October were also characterized by high concentrations of EC and $P_2O_5$. These results indicate that the sampling time for soil and water can significantly influence the evaluation of soil properties with different farming methods.

• Animal Bioscience
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• 제34권2호
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• pp.185-191
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• 2021

Objective: The objective of this study was to estimate the genetic parameters for worm resistance (WR) and associated characteristics, using the linear-threshold animal model via Bayesian inference in single- and multiple-trait analyses. Methods: Data were collected from a herd of Santa Inês breed sheep. All information was collected with animals submitted to natural contamination conditions. All data (number of eggs per gram of feces [FEC], Famacha score [FS], body condition score [BCS], and hematocrit [HCT]) were collected on the same day. The animals were weighed individually on the day after collection (after 12-h fasting). The WR trait was defined by the multivariate cluster analysis, using the FEC, HCT, BCS, and FS of material collected from naturally infected sheep of the Santa Inês breed. The variance components and genetic parameters for the WR, FEC, HCT, BCS, and FS traits were estimated using the Bayesian inference under the linear and threshold animal model. Results: A low magnitude was obtained for repeatability of worm-related traits. The mean values estimated for heritability were of low-to-high (0.05 to 0.88) magnitude. The FEC, HCT, BCS, FS, and body weight traits showed higher heritability (although low magnitude) in the multiple-trait model due to increased information about traits. All WR characters showed a significant genetic correlation, and heritability estimates ranged from low (0.44; single-trait model) to high (0.88; multiple-trait model). Conclusion: Therefore, we suggest that FS be included as a criterion of ovine genetic selection for endoparasite resistance using the trait defined by multivariate cluster analysis, as it will provide greater genetic gains when compared to any single trait. In addition, its measurement is easy and inexpensive, exhibiting greater heritability and repeatability and a high genetic correlation with the trait of resistance to worms.

• 현장농수산연구지
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• 제7권1호
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• pp.81-89
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• 2005

포도 '캠벨얼리'에서 석회비료를 과면살포한 후, 과피의 T-N, P, K, Ca 및 Mg 함량은 무처리과에 비하여 석회비료 전 처리 모두 큰 차이는 없었다. 과방중, 과립중, 과방장 및 산 함량은 처리 간 차이가 없었으나, 가용성고형물은 봉지가 씌워진 상태로 액상석회비료를 처리한 과실은 다른 처리에 비하여 감소하였다. 과피의 안토시아닌 함량은 무처리과에 비하여 소석회 및 봉지가 씌워진 상태로 액상석회비료를 처리한 과실이 현저히 감소하였다. 과피의 과분은 소석회 및 패화석 분말비료 처리과는 무처리과에 비하여 현저히 적게 발생되었다. 과립의 경도는 액상석회비료 및 소석회 처리과가 현저히 증가하였다. 그러나 과립의 탄력성은 패화석 분말비료 처리과는 현저히 감소하였다. 또한 상온 저장 중 감랑은 전 처리 모두 차이를 인정할 수 없었다.

• 농업과학연구
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• 제49권4호
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• pp.973-981
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• 2022

'Shine Muscat' (Vitis labruscana Bailey x Vitis vinifera L.) grapes recently have been greatly favored in Korea, and as of 2022, account for 38.6% of the total grape market in Korea. However, there is a lack of research on post-harvest quality control appropriate to the unique characteristics of 'Shine Muscat'. In order to continuously increase domestic demand and exports in the future, it is essential to establish proper postharvest techniques. Essential oils have remarkable potential as biologically active and environmentally friendly antiseptics for the fruit industry. The objective of this study is to evaluate the effects of essential oils and aroma constituent treatment on fruit quality attributes of 'Shine Muscat' grapes in order to extend fruit quality during a simulated export period. A cluster of 'Shine Muscat' grapes was placed in a newly developed paper box container, and treated with rosemary essential oil, thyme essential oil, and linalool, along with an untreated control. The results showed no significant effect on fruit quality indices, such as cluster weight loss, berry firmness, soluble solids content, titratable acidity, sugar acid ratio, and color change, but showed a positive effect on the alleviation of berry shatter compared to the untreated control. In addition, rosemary oil treatment reduced the proportion of unhealthy berries. Therefore, the results indicated that the newly developed packaging container along with certain essential oil treatment could be applied as a promising packaging material for table grape export.

• Asian-Australasian Journal of Animal Sciences
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• 제16권2호
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• pp.277-281
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• 2003

Amplified fragment length polymorphism (AFLPs) markers were used to investigate the genetic variation in six autochthonous goat populations distributed in the middle and lower Yangtze River valley. The goat populations were Chengdu Grey Goat (CGG), Chuandong White Goat (CWG), Banjiao Goat (BG), Matou Goat (MG), Hui Goat (HG) and Yangtze River Delta White Goat (YRDWG). A total of 180 individuals (30 per population) were analysed using ten selected AFLP primer combinations that produced 78 clear polymorphism loci. The variability at AFLP loci was largely maintained within populations, as indicated by the average genetic similarity, and they were ranged from 0.745 to 0.758 within populations and 0.951 to 0.970 between populations. No breed specific markers were identified. Cluster analysis based on Nei' genetic distance between populations indicated that Chengdu Grey Goat is the most distant population, while CWG and YROWG were the closest populations, followed by BG, HG and MG. Genetic diversity of the goat populations didn' confirm what was expected on the basis of their geographical location, which may reflect undocumented migrations and gene flows and identify an original genetic resource.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.94.2-95
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• 2003

E. intermedium 60-2G possessing a strong ability to solubilize insoluble phosphate, has plant growth-promoting activity, induced systemic resistance activity against scab pathogen in cucumber, and antifungal activity against various phytopathogenic fungi. The phosphate solubilizing activity of 60-2G may be mainly accomplished by production of gluconic acid through a direct extracellular oxidation of glucose by glucose dehydrogenase that required a PQQ cofactor for its activation. A pqq gene cluster conferred Phosphate-solubilizing activity in E. coli DH5${\alpha}$ was cloned and sequenced. The 6,783 bP pqq sequence had six open reading frames (from A to F) and showed 50-95％ homology to pqq genes from other bacteria. The E. coli strain expressing the pqq genes solubilized phosphate from hydroxyapatite after a pH drop to 4.0, which paralleled in time the secretion of gluconic acid. To study the role of PQQ in biocontrol traits of E. intermedium, PQQ mutants of 60-2G were constructed by marker exchangee mutagenesis. The PQQ mutants of E. intermedium were lost activities of solubilizing phosphate, growth inhibition of phytopathogenic fungi, and plant growth promotion. These findings suggest that PQQ plays an important role, possibly activation of certain enzymes, in several beneficial bacterial traits of E. intermedium by as yet an unknown mechanism.