• The Korean Journal of Mycology
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• v.25 no.4 s.83
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• pp.362-368
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• 1997

Five formae speciales of Fusarium oxysporum in Korea were examined using RFLP analysis to find the possibility for classification and analyze genetic variations. DNAs from F. oxysporum f. sp. lycopersici, cucumerinum, fragariae, garlic and sesami were used with three recombinant probes such as pFC46, pFC52 and pFC57. Distinct differences among five formae speciales of this fungus were detected in RFLP band patterns based on southern hybridization of genomic DNA using each recombinant clone, which was a repetitive copy probe. Strains belong to four formae speciales could be very stable in genetic variation except f. sp. sesami which has more variation than the others based on the RFLP analysis. They formed their own cluster which has high similarity within the same formae specialis resulted from the UPGMA analysis for genetic relationship analysis and each cluster represented its own formae specialis. The method using three recombinant DNA probes could be a good tool for classification of formae speciales in F. oxysporum.

• Korean Journal of Weed Science
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• v.8 no.1
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• pp.9-14
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• 1988

Three varieties of Echinochloa crus-galli (L.) Beauv. and five collections different in gross morphology from the E. crus-galli species were obtained in a lowland rice field located in Jukjeol-Ri, Soyang-Myeon, Wanju-Kun, Jeonbug to compare the ecological and physiological characteristics. There were great variations in seed dormancy, relative growth rates, days required to panicle emergence and panicle morphology among the collections. On the basis of the cluster analysis using the different characteristics, the collections were divided into three groups; E. crus-galli var. frumentaceae and its two ecotypcs, E. crus-galli var. echinata and its two ecotypes, and E crus-galli var. crus-galli and its one ecotype. There were differential responses in percent germination and post-germination growth of the collections to butachlor (2-chloro-2', 6'-diethyl-N-butoxymethyl acetanilide).

• Horticultural Science & Technology
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• v.34 no.1
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• pp.15-23
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• 2016

A study was conducted to evaluate the effects of irrigation (amount, interval) on tree growth, fruit quality, and cracking of organic 'Campbell Early' grapevine in 2012 and 2013. Three irrigation treatments were applied using a sprinkler system from mid-June to mid-August in 2012 and 2013, as follows: 10 mm was applied daily (10 mm-IR), 20 mm was applied every two days (20 mm-IR), and 30 mm was applied every five days (30 mm-IR). Soil electrical conductivity (EC) and temperature were found to be greatest in the 10 mm-IR treatment in both years. Soil moisture content ranged between 20-40% in the 10 mm-IR, between 20-60% in the 20 mm-IR, and between 20-70% in the 30 mm-IR treatment plots. The total number of leaves per shoot and shoot growth were found to be greatest in the 20 mm-IR and 30 mm-IR treatments, respectively. Cluster and berry weights, and cluster and berry sizes were not consistently affected by the treatments. The 10 mm-IR treatment resulted in an increase in fruit SSC, SSC/acidity ratio, and berry skin pigmentation ($b^*$; blue). Approximately 5% of fruit cracking was observed on average over both years in the 10 mm-IR-treated fruit, while the 30 mm-IR treatment resulted in nearly 18% of cracking in 2012. Average marketable fruit yield per year over two years was greatest for the 10 mm-IR treatment ($24.4t{\cdot}ha^{-1}$) followed by the 30 mm-IR treatment ($22.7t{\cdot}ha^{-1}$) and lastly the 20 mm-IR treatment ($22.2t{\cdot}ha^{-1}$). Thus, the 10 mm-IR treatment represents a suitable irrigation regimen for controlling leaf and shoot growth of vines grown under a rain-shelter system in sandy loam soils, while improving fruit sugar contents and skin color and limiting fruit cracking.

• Environmental Engineering Research
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• v.24 no.1
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• pp.45-53
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• 2019

Water and soil properties in paddy fields subjected to organic and conventional farming were characterized over the rice cycle in South Korea. To achieve the goals of this study, we sampled and analyzed soil and water from 24 organic paddy plots and 11 conventional paddy plots in March, May, August, and October 2016. The results were analyzed using statistical analyses, including analysis of variance (ANOVA), cluster analysis, and principal component analysis. The ANOVA results showed that water content (WC), electrical conductivity (EC), organic matter (OM), and available phosphorus ($P_2O_5$) in soil varied significantly (p < 0.01) depending on the farming method. Higher OM, EC, and $P_2O_5$ of soil were observed in the conventional paddies than in the organic paddies. All soil properties, except pH and ammonium, depended on seasonal variation. Cluster analysis revealed that soil properties in May were distinctly separated from those in other seasons mainly due to basal fertilization. The principal component analysis distinguished the soil properties in different seasons, but such a distinction was not observed between the soil properties in organic and conventional paddies. Low contents of WC, OM, and total N were observed in March. High concentrations of nitrate and total P were observed in May, but these were low in August and October. The soils from October were also characterized by high concentrations of EC and $P_2O_5$. These results indicate that the sampling time for soil and water can significantly influence the evaluation of soil properties with different farming methods.

• Animal Bioscience
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• v.34 no.2
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• pp.185-191
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• 2021

Objective: The objective of this study was to estimate the genetic parameters for worm resistance (WR) and associated characteristics, using the linear-threshold animal model via Bayesian inference in single- and multiple-trait analyses. Methods: Data were collected from a herd of Santa Inês breed sheep. All information was collected with animals submitted to natural contamination conditions. All data (number of eggs per gram of feces [FEC], Famacha score [FS], body condition score [BCS], and hematocrit [HCT]) were collected on the same day. The animals were weighed individually on the day after collection (after 12-h fasting). The WR trait was defined by the multivariate cluster analysis, using the FEC, HCT, BCS, and FS of material collected from naturally infected sheep of the Santa Inês breed. The variance components and genetic parameters for the WR, FEC, HCT, BCS, and FS traits were estimated using the Bayesian inference under the linear and threshold animal model. Results: A low magnitude was obtained for repeatability of worm-related traits. The mean values estimated for heritability were of low-to-high (0.05 to 0.88) magnitude. The FEC, HCT, BCS, FS, and body weight traits showed higher heritability (although low magnitude) in the multiple-trait model due to increased information about traits. All WR characters showed a significant genetic correlation, and heritability estimates ranged from low (0.44; single-trait model) to high (0.88; multiple-trait model). Conclusion: Therefore, we suggest that FS be included as a criterion of ovine genetic selection for endoparasite resistance using the trait defined by multivariate cluster analysis, as it will provide greater genetic gains when compared to any single trait. In addition, its measurement is easy and inexpensive, exhibiting greater heritability and repeatability and a high genetic correlation with the trait of resistance to worms.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.973-981
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• 2022

'Shine Muscat' (Vitis labruscana Bailey x Vitis vinifera L.) grapes recently have been greatly favored in Korea, and as of 2022, account for 38.6% of the total grape market in Korea. However, there is a lack of research on post-harvest quality control appropriate to the unique characteristics of 'Shine Muscat'. In order to continuously increase domestic demand and exports in the future, it is essential to establish proper postharvest techniques. Essential oils have remarkable potential as biologically active and environmentally friendly antiseptics for the fruit industry. The objective of this study is to evaluate the effects of essential oils and aroma constituent treatment on fruit quality attributes of 'Shine Muscat' grapes in order to extend fruit quality during a simulated export period. A cluster of 'Shine Muscat' grapes was placed in a newly developed paper box container, and treated with rosemary essential oil, thyme essential oil, and linalool, along with an untreated control. The results showed no significant effect on fruit quality indices, such as cluster weight loss, berry firmness, soluble solids content, titratable acidity, sugar acid ratio, and color change, but showed a positive effect on the alleviation of berry shatter compared to the untreated control. In addition, rosemary oil treatment reduced the proportion of unhealthy berries. Therefore, the results indicated that the newly developed packaging container along with certain essential oil treatment could be applied as a promising packaging material for table grape export.

• Journal of Practical Agriculture & Fisheries Research
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• v.7 no.1
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• pp.81-89
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• 2005

The experiment was conducted to evaluate the effect of fruit skin spray of the lime fertilizer in 'Campbell Early' grapes. The T-N, P, K, Ca and Mg of fruit skin and cluster weight, berry weight, cluster length showed no difference between control and lime fertilizer. But soluble solids in fruit was reduced by fruit skin spray of liquid calcium fertilizer extracted from oyster shell after bagging(LCaB). The anthocyanin content of fruit skin was decreased by air-slaked lime(HCa) and LCaB treatment. Also, occurrence of fruit skin bloom was significantly reduced by HCa and oyster shell powder(OS-CaP) treatment. The berry firmness was significantly increased through liquid calcium fertilizer extracted from oyster shell(LCa) and HCa treatment. Conspicuously, berry elasticity was rather decreased by OS-CaP treatment. However, there was no difference of weight loss of fruit among treatments during room temperature storage.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.2
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• pp.277-281
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• 2003

Amplified fragment length polymorphism (AFLPs) markers were used to investigate the genetic variation in six autochthonous goat populations distributed in the middle and lower Yangtze River valley. The goat populations were Chengdu Grey Goat (CGG), Chuandong White Goat (CWG), Banjiao Goat (BG), Matou Goat (MG), Hui Goat (HG) and Yangtze River Delta White Goat (YRDWG). A total of 180 individuals (30 per population) were analysed using ten selected AFLP primer combinations that produced 78 clear polymorphism loci. The variability at AFLP loci was largely maintained within populations, as indicated by the average genetic similarity, and they were ranged from 0.745 to 0.758 within populations and 0.951 to 0.970 between populations. No breed specific markers were identified. Cluster analysis based on Nei' genetic distance between populations indicated that Chengdu Grey Goat is the most distant population, while CWG and YROWG were the closest populations, followed by BG, HG and MG. Genetic diversity of the goat populations didn' confirm what was expected on the basis of their geographical location, which may reflect undocumented migrations and gene flows and identify an original genetic resource.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.94.2-95
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• 2003

E. intermedium 60-2G possessing a strong ability to solubilize insoluble phosphate, has plant growth-promoting activity, induced systemic resistance activity against scab pathogen in cucumber, and antifungal activity against various phytopathogenic fungi. The phosphate solubilizing activity of 60-2G may be mainly accomplished by production of gluconic acid through a direct extracellular oxidation of glucose by glucose dehydrogenase that required a PQQ cofactor for its activation. A pqq gene cluster conferred Phosphate-solubilizing activity in E. coli DH5${\alpha}$ was cloned and sequenced. The 6,783 bP pqq sequence had six open reading frames (from A to F) and showed 50-95％ homology to pqq genes from other bacteria. The E. coli strain expressing the pqq genes solubilized phosphate from hydroxyapatite after a pH drop to 4.0, which paralleled in time the secretion of gluconic acid. To study the role of PQQ in biocontrol traits of E. intermedium, PQQ mutants of 60-2G were constructed by marker exchangee mutagenesis. The PQQ mutants of E. intermedium were lost activities of solubilizing phosphate, growth inhibition of phytopathogenic fungi, and plant growth promotion. These findings suggest that PQQ plays an important role, possibly activation of certain enzymes, in several beneficial bacterial traits of E. intermedium by as yet an unknown mechanism.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.142-143
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• 2003

Ammi majus (white lace flower, Unbelliferae) is an ornamental plant used for cut-flower arrangements worldwide. A potyvirus was isolated from its leaves with mosaic and chlorotic symptoms in the cultivated field of Chiba, Japan. Compared with Japanese homuort mosaic virus (JHMV) previously isolated from Cryptotaenia japonica, it showed similar characteristics in host reactions and molecular properties. The nucleotide sequences of coat protein and 3'- nontranslated region were highly homologous and shared 87％ and 91％ identities with those of JHMV, respectively. This virus was thus supposed to be an isolate of JHMV and designated as JHMV-Am. Phylogenetic tree was constructed using CP nucleotide sequences of the two isolates and other potyviruses previously reported. JHMV-Am and JHMV fell into a cluster with Korean strain of Zantedeschia mosaic virus (ZaMV-KR). However, low identity in amino acid sequences was found in the termini of CP genes between the two isolates of JHMV and ZaMV-KR.