• Journal of Life Science
• /
• v.22 no.6
• /
• pp.815-822
• /
• 2012

Piceatannol (trans-3,4,3',5'-tetrahydroxystilbene), a natural stilbene, is an analogue of resveratrol. Although recent experimental data have revealed the health benefit potency of piceatannol, the molecular mechanisms underlying the anti-cancer activity have not yet been studied in detail. In the present study, the further possible mechanisms by which piceatannol exerts its pro-apoptotic action in cultured human lung cancer A549 cells were investigated. Exposure of A549 cells to piceatannol resulted in growth inhibition and induction of apoptosis. Apoptosis induction of A549 cells by piceatannol showed correlation with proteolytic activation of caspase-3, -8, and -9, and concomitant degradation of activated caspase-3 target proteins such as poly (ADP-ribose) polymerase, phospholipase C-${\gamma}1$, ${\beta}$-catenin, and Inhibitor caspase-activated DNase. The increase in apoptosis by piceatannol treatment was also associated with an increase of pro-apoptotic Bax expression and decrease of anti-apoptotic Bcl-2 and Bcl-xL expression, and caused down-regulation of the inhibitor of apoptosis protein family members and up-regulation of Fas and Fas legend. In addition, piceatannol treatment markedly inhibited the expression of mRNA and proteins of inducible nitric oxide (NO) synthase, and the levels of NO production were progressively down-regulated by piceatannol treatment in a dose-dependent fashion. The results indicate that piceatannol may have therapeutic potential against human gastric cancer cells.

• Journal of Korean Society of Water and Wastewater
• /
• v.29 no.4
• /
• pp.511-517
• /
• 2015

Korea's modern waterworks began with construction of DDukdo water treatment plant in 1908 and has been growing rapidly along with the country's economic development. As a result, water supply rates have reached 98.5% based on 2013. Despite multilateral efforts for high-quality water supply, such as introduction of advanced water treatment process, expansion of waterworks infrastructure and so on, distrust for drinking tap water has been continuing and domestic consumption rate of tap water is in around 5% level and extremely poor comparing to advanced countries such as the United States(56%), Japan(52%), etc. Recently, the water management has been facing the new phase due to water environmental degradation caused by climate change, aging facilities, etc. Therefore, K-water has converted water management paradigm from the "clean and safe water" to the "healthy water" and been pushing the Smart Water City(SWC) Pilot Project in order to develop and spread new water supply models for consumers to believe and drink tap water through systematic water quality and quantity management combining ICT in the whole water supply process. The SWC pilot projects in Pa-ju city and Go-ryeong county were an opportunity to check the likelihood of the "smart water management" as the answer to future water management. It is needed to examine the necessity of smart water management introduction and nationwide SWC expansion in order to improve water welfare for people and resolve domestic & foreign water problems.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.38 no.10
• /
• pp.1133-1137
• /
• 2014

The ultrasonic nonlinear parameter ${\beta}$ is generally known as an effective parameter for evaluating material degradation. Thus far, most research has been conducted using a contact method. However, since measurement by this contact method is affected by the contact conditions between the transducer and the specimen, additional devices are required to maintain the contact conditions stable during the measurement. To avoid this inconvenience, this paper proposes a noncontact method. In this study, only the receiver was replaced with a noncontact receiver, and then, the ultrasonic nonlinear parameters measured by the newly developed noncontact receiver were compared with those measured by the contact receiver. Results obtained using both these receivers for heat-treated aluminum alloy specimens showed good agreement. From this result, we can confirm that the ultrasonic nonlinear parameter ${\beta}$ can be measured using the proposed noncontact ultrasonic method.

• Journal of Soil and Groundwater Environment
• /
• v.18 no.2
• /
• pp.1-9
• /
• 2013

The landfarming treatment for the remediation of the petroleum contaminated soil at the returned U.S. Military bases was investigated in this study. Specifically, the bioaugmentation performance using various commercially available petroleum-degrading bacteria was evaluated and the directions for enhancing the performance of the landfarming treatment were suggested. The environmental factors of the soils at the returned U.S. Military bases chosen for remediation indicate that the landfarming treatment can be used as the remediation technique; however, the addition of nitrogen or phosphorus is required. The lab-scale landfarming treatment tests using the model soil and the site soil showed that the degradation efficiency was greater with the model soil than the site soil and that the treatment performance was not affected by the number of bacteria present in the soil in the range of $10^6-10^{12}$ CFU/g. These results suggest that the successful landfarming treatment depends on the petroleum degradability of bacteria used and the environmental conditions during the treatment rather than the number of petroleum-degrading bacteria used.

• Journal of the Korean Magnetics Society
• /
• v.3 no.3
• /
• pp.229-233
• /
• 1993

The effects of differential partial pressures of oxygen during sputtering on the magnetic and recording characteristics of magneto-optical disks were investigated. Different flows of oxygen were deliberately introduced into the sputtering chamber to have a variety of partial pressures of oxygen during sputtering. A residual gas analyzer was employed to monitor the oxygen peak before, during, and after sputtering and to estimate the reacted oxygen amount. Most of the oxygen introduced into the chamaber was reacted during sputtering. As the partial pressure of oxygen increased, the oxygen content of the TbFeCoCr film increased also. The oxygen appeared to be bound as Tb-O, effectively decreasing the magnetically active Tb content of the film The coercivity decreased but the squareness of the Kerr hysteresis loops was still excellent. The perpendicular anisotropy was not significantly affected by oxygen amount. The carrier-to-noise ratio, includi!1g the write power sensitivity and bias field sensitivity did not change too much with oxygen content in the film The disks sputtered with oxygen showed better bias field sensitivity with lower write power threshold than the disk sputtered without oxygen, due to high demagnetization during domain formation. No significant degradation of coercivity for the disk sputtered oxygen was observed during an accelerated aging test.

• Journal of Technologic Dentistry
• /
• v.36 no.1
• /
• pp.51-62
• /
• 2014

Purpose: Recently our country is rapidly aging population is growing. In the oral cavity of the elderly status of oral health-related quality of life to evaluate any impact. Methods: The survey used structured self administered questionnaires from April to May in 2011 in Daejeon and Chungcheong provinces to 277 people, analysis of the general characteristics of a frequency analysis method, the difference between the quality of life T/F test, whether oral self-according to the quality of life healthy oral health effects of the multiple regression analysis. Results: Award comes on the number of residual value, lower age all the more, smaller monthly income was small, but significant difference between them was no difference between gender. Residual value according to the number of differences in the quality of life of physical pain upper, physical disability, the lower the physical pain, physical disability, psychological disability were significant differences in degradation. Depending on oral maxillary prosthesis fitted to physical pain, psychological discomfort, physical disability, the lower the functional limitation, physical pain, there was a significant difference in physical disability. Conclusion: Status of the oral cavity of the elderly factors affecting the quality of life remaining in the lower dimensions, upper and lower prosthetics, self-aware state of oral health in order to improve the quality of life of elderly oral health education to be strengthened to increase the residual value, reducing their own prosthetic perceived oral health is health, so they feel it should be for the development of health education programs for the elderly should be.

• KIEAE Journal
• /
• v.14 no.1
• /
• pp.121-129
• /
• 2014

As time passes, the aging of the plant building, the building's energy performance degradation than the initial plan does not express a situation could arise. This year, the certification of buildings certified in 2009 has expired for measures such as the situation required. In this study, management of national and international green building certification and re-certification was compared in two ways. First, the evaluation of green building certification system management assessments were compared. Second, the green building certification system for the re-certification analysis. As a result, G-SEED was not reflected life-cycle of building in management assessment and the commissioning of G-SEED is the UK and the U.S and other concepts of evaluation. In addition, the re-certification system is insufficient about substantial energy consumption of buildings. In this study, the revised the management assessments in conjunction with the re-certification system to manage the building is proposed to improve. In addition, the current evaluation of the existing building certification "existing building" and "building the first certified" as it is more efficient to separate the information into assessment was judged. Green building certification system to meet the purpose of management and operation, and disposal phases of the building to promote energy conservation and sustainability in order to the management a systematic and detailed evaluation and re-certification system developed for the revision of the specific items required and future research want to continue.

• Journal of the Korean Institute of Gas
• /
• v.2 no.4
• /
• pp.42-46
• /
• 1998

Miniaturized specimen technology Permits mechanical behavior to be determined using a minimum volume of material. The technology is useful in case of not collecting a large amount of materials from industrial equipments. Five kinds of accelerated degradation materials were prepared by isothermal aging heat treatment at $630^{\circ}C$. Three kinds of specimens were prepared for impact testing. In order to increase plastic constraint of subsize specimen, side-groove was introduced. Results between subsize and full size impact testing were compared. Size effects correlations were developed for the impact properties of turbine rotor material. These correlations successfully predict the ductile brittle transition temperature (DBTT) of full size Charpy impact specimens based on subsize specimen data.

• Molecules and Cells
• /
• v.40 no.9
• /
• pp.643-654
• /
• 2017

Autophagy is a degradation pathway in eukaryotic cells in which aging proteins and organelles are sequestered into double-membrane vesicles, termed autophagosomes, which fuse with vacuoles to hydrolyze cargo. The key step in autophagy is the formation of autophagosomes, which requires different kinds of vesicles, including COPII vesicles and Atg9-containing vesicles, to transport lipid double-membranes to the phagophore assembly site (PAS). In yeast, the cis-Golgi localized t-SNARE protein Sed5 plays a role in endoplasmic reticulum (ER)-Golgi and intra-Golgi vesicular transport. We report that during autophagy, sed5-1 mutant cells could not properly transport Atg8 to the PAS, resulting in multiple Atg8 dots being dispersed into the cytoplasm. Some dots were trapped in the Golgi apparatus. Sed5 regulates the anterograde trafficking of Atg9-containing vesicles to the PAS by participating in the localization of Atg23 and Atg27 to the Golgi apparatus. Furthermore, we found that overexpression of SFT1 or SFT2 (suppressor of sed5 ts) rescued the autophagy defects in sed5-1 mutant cells. Our data suggest that Sed5 plays a novel role in autophagy, by regulating the formation of Atg9-containing vesicles in the Golgi apparatus, and the genetic interaction between Sft1/2 and Sed5 is essential for autophagy.

• Nutrition Research and Practice
• /
• v.8 no.4
• /
• pp.398-403
• /
• 2014

BACKGROUND/OBJECTIVES: Ultraviolet B (UVB) irradiation on skin can induce production of reactive oxygen species (ROS), which cause expression of matrix metalloproteinases (MMPs) and collagen degradation. Thus, chronic exposure of skin to UVB irradiation leads to histological changes consistent with aging, such as wrinkling, abnormal pigmentation, and loss of elasticity. We investigated the protective effect of the standardized green tea seed extract (GSE) on UVB-induced skin photoaging in hairless mice. MATERIALS/METHODS: Skin photoaging was induced by UVB irradiation on the back of Skh-1 hairless mice three times per week and UVB irradiation was performed for 10 weeks. Mice were divided into six groups; normal control, UVB irradiated control group, positive control (UVB + dietary supplement of vitamin C 100 mg/kg), GSE 10 mg/kg (UVB + dietary supplement of GSE 10 mg/kg), GSE 100 mg/kg (UVB + dietary supplement of GSE 100 mg/kg), and GSE 200 mg/kg (UVB + dietary supplement of GSE 200 mg/kg). RESULTS: The dietary supplement GSE attenuated UVB irradiation-induced wrinkle formation and the decrease in density of dermal collagen fiber. In addition, results of the antioxidant analysis showed that GSE induced a significant increase in antioxidant enzyme activity compared with the UVB irradiation control group. Dietary supplementation with GSE 200 mg/kg resulted in a significant decrease in expression of MMP-1, MMP-3, and MMP-9 and an increase in expression of TIMP and type-1 collagen. CONCLUSIONS: Findings of this study suggest that dietary supplement GSE could be useful in attenuation of UVB irradiation-induced skin photoaging and wrinkle formation due to regulation of antioxidant defense systems and MMPs expression.