• 대한한의학방제학회지
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• 제16권2호
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• pp.183-191
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• 2008

Acetaminophen (N-acety1-p-aminophenol, paracetamol) is widely used as an over-the-counter analgesic and antipyretic drug. Intake of a over dose of acetaminophen may result in severe hepatic necrosis. In this study, we investigated the liver damage in mice using single dose (300 mg/kg) of acetaminophen and the possible protective effects of administration (50-200 mg/kg body weight) of SB-Ex on acetaminophen-induced liver damage in mice. The alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities were determined in the plasma of mice. The effect of SB-Ex on lipid peroxidation product thiobarbituric reacting substances (TBARS) and some antioxidant enzymes superoxide dismutase (SOD), catalase, d-aminolevulinate dehydratase (${\sigma}$-ALA-D) activities, and gluthathione peroxidase (GPx), were also evaluated in the mouse liver homogenate. Acetaminophen caused liver damage as evident by statistically significant increased in plasma activities of AST and ALT. There were general statistically significant losses in the activities of SOD, catalase, ${\sigma}$-ALA-D, and GPx and an increase in TBARS in the liver of acetaminophen-treated group compared with the control group. However, SB-Ex was able to counteract these effects. These results suggest that SB-Ex can act as hepatoprotectives against acetaminophen toxicity and is a good candidate for further evaluation as an effective chemotherapeutic agent.

• Preventive Nutrition and Food Science
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• 제7권2호
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• pp.113-118
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• 2002

Alpha-lipoic acid is a known hypoglycemic agent that may be useful in the treatment of diabetes. The objective of this study was to investigate the fate of glucose in isolated muscles incubated with lipoic acid by determining its direct effects on specific metabolic and signaling pathways. Soleus muscles from healthy rats were incubated with lipoic acid in the absence or presence of insulin. Glucose transport, glycogen synthesis, glucose oxidation and lipid synthesis were determined and affects on major pathways associated with insulin signaling were evaluated. Glucose transport was not significantly altered by the addition of lipoic acid to the incubation medium. However, lipoic acid decreased glycogen synthesis in comparison to controls. Glucose oxidation was moderately increased while de-novo lipid synthesis from glucose was inhibited. Wortmannin repressed insulin stimulation of glucose incorporation into glycogen, an effect that was augmented by the combined treatment of wortmannin and lipoic acid. Basal and insulin-stimulated serine phosphorylation of Akt was not changed by the addition of lipoic acid to the incubation medium. These data show that in this in vitro model, lipoic acid did not significantly affect glucose uptake but dramatically modified pathways of glucose metabolism within muscle tissue.

• 한국조사연구학회:학술대회논문집
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• 한국조사연구학회 2000년도 춘계학술대회 조사연구의 방법론적 쟁점
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• pp.59-87
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• 2000

기업체에서 변화 활동을 담당하는 변화 담당자 305명을 대상으로 이들의 자기관, 모험 수용성과 같은 성격특성이 조직이 추진하는 변화활동 및 담당업무에 대한 적용에 미치는 효과를 살펴보았다. 연구 결과, 긍정적인 자기관과 모험 수용성이 높은 사람들은 조직이 추진하고 있는 변화 활동관련 업무를 보다 적극적으로 수행하고 있었으며, 담당하는 변화관련 업무에 대한 적응도가 높은 것으로 나타났다. 또한 변화대응성은 긍정적인 자기관 및 모험 수용성과 업무에 대한 적응간을 매개하는 변수역할을 원활하게 수행하기 위해서는 긍정적인 자기관과 모험 수용성을 보유하는 것이 필요하며, 조직에서 변화 담당자를 선발하고 이들을 교육할 때 이들의 자기관을 긍정적으로 변화시켜주고, 모험 수용성을 높여주는 것(또는 이런 성향이 높은 사람을 선발하는 것)이 바람직함을 시사한다. 또한 변화담당자들이 자신들이 담당하는 업무에 쉽게 적응하도록 하기 위해서는 이들의 변화대응성을 높이는 것이 필요함을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제28권10호
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• pp.1626-1634
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• 2018

Ginseng (the root of Panax ginseng Meyer) fermented by Lactobacillus plantarum has been found to attenuate allergic responses in in vitro and in vivo experimental models. Ginseng has been reported to also possess various biological functions including anti-inflammatory activity. The present study was aimed at comparing the anti-allergic effect of ginseng and fermented ginseng extracts on IgE-mediated passive cutaneous anaphylaxis in vitro in a murine cell line and in vivo in mice. Fermented ginseng extract (FPG) showed higher inhibitory effect against in vitro and in vivo allergic responses when compared with ginseng extract (PG). The secretion of ${\beta}$-hexosaminidase and interleukin (IL)-4 from the IgE-DNP-stimulated RBH-2H3 mast cells were significantly (p < 0.05) inhibited by FPG treatment, and this effect was concentration-dependent. Further, MKK4 activation and subsequent JNK phosphorylation were attenuated by FPG treatment. The inhibitory effect of FPG on the in vitro allergic response was verified in vivo against IgE-DNP-induced passive cutaneous anaphylaxis in a mouse model. These data indicated that the fermentation of ginseng with L. plantarum enhanced its anti-allergic effects both in vitro and in vivo. We predict that compositional changes in the ginsenosides caused by the fermentation may contribute to the change in the anti-allergic effects of ginseng. The results of our study highlight the potential of the use of FPG as a potential anti-allergic agent.

• 한국컴퓨터산업학회논문지
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• 제3권2호
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• pp.209-216
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• 2002

웹은 브라우저를 통한 단순한 정보의 전달과 정보의 공유수단으로서가 아니라, 수많은 컴퓨터 자원이 연결되어 있는 병렬 컴퓨팅을 위한 기반구조로서 이용될 수 있는 잠재적인 가능성을 가지고 있다. 웹을 기반으로 한 병렬컴퓨팅의 접근방법은 기존의 다른 방법들에 비하여 일반 사용자들의 접근에 대한 용이성, 확장성, 비용대비 효과적인 병렬시스템 구축의 용이성, 기존의 네트워크를 활용할 수 있다는 측면에서 많은 장점을 가진다. 자바언어에서의 이동코드(mobile code)의 개념을 가지고 있는 applet은 많은 계산을 필요로 하는 프로그램이 독립된 병렬작업으로 분할되어 웹 상의 여러 노드들로 이동되어 실행이 되어질 수 있는 가능성을 제시하여 주고 있다. 그러나 자바 applet은 보안에 대한 모델상의 제약으로 인하여 제한된 범위 내에서만 실행이 가능하며 클라이언트가 applet을 포함하고 있는 호스트들에 접속을 해야 한다는 점에서 유연성이 부족하다. 따라서 본 논문에서는 applet의 개념에 자치적으로 작업을 처리할 수 있는 기능을 추가하여 이동형 에이전트라 할 수 있는 Aglet(Agile applet)을 이용하여 웹 기반 병렬 컴퓨팅 환경을 설계하였으며, 웹 기반 병렬컴퓨팅 환경을 구축할 때 필요한 기술과 구조가 분석되었다. 또한 applet 기반의 방식과 비교하여 간단한 시뮬레이션과 분석이 이루어졌다.

• The Korean Journal of Physiology and Pharmacology
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• 제22권5호
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• pp.525-538
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• 2018

Post-traumatic stress disorder (PTSD) is a trauma-induced psychiatric disorder characterized by impaired fear extermination, hyperarousal, and anxiety that may involve the release of monoamines in the fear circuit. The reported pharmacological properties of tetramethylpyrazine (TMP) include anti-cancer, anti-diabetic, anti-atherosclerotic, and neuropsychiatric activities. However, the anxiolytic-like effects of TMP and its mechanism of action in PTSD are unclear. This study measured several anxiety-related behavioral responses to examine the effects of TMP on symptoms of anxiety in rats after single prolonged stress (SPS) exposure by reversing the serotonin (5-HT) and hypothalamic-pituitary-adrenal (HPA) axis dysfunction. Rats were given TMP (10, 20, or 40 mg/kg, i.p.) for 14 days after SPS exposure. Administration of TMP significantly reduced grooming behavior, increased the time spent and number of visits to the open arm in the elevated plus maze test, and significantly increased the number of central zone crossings in the open field test. TMP administration significantly reduced the freezing response to contextual fear conditioning and significantly restored the neurochemical abnormalities and the SPS-induced decrease in 5-HT tissue levels in the prefrontal cortex and hippocampus. The increased 5-HT concentration during TMP treatment might be partially attribute to the tryptophan and 5-hydroxyindoleacetic acid mRNA level expression in the hippocampus of rats with PTSD. These findings support a role for reducing the altered serotonergic transmission in rats with PTSD. TMP simultaneously attenuated the HPA axis dysfunction. Therefore, TMP may be useful for developing an agent for treating psychiatric disorders, such those observed in patients with PTSD.

• Archives of Plastic Surgery
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• 제35권2호
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• pp.121-126
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• 2008

Purpose: Poly-N-acetyl glucosamine(PGlcNAc) nanofiber-based materials, produced by a marine microalga, have been characterized as effective hemostatic and angiogenic agents. The similarity between PGlcNAc patch and the natural extracellular matrix allows it to support new healthy tissue growth in an injured area and to encourage fluid absorption. In this study, we hypothesized that a poly-N-acetyl glucosamine fiber patch(PGlcNAc patch) may enhance wound healing in the db/db mouse. Methods: PGlcNAc patches were applied on one square centimeter, full-thickness, skin wounds in the db/db mouse model. Wounds(n=15 per group) were dressed with a PGlcNAc nanofiber patch for 1 hour(1 h), 24 hours(24 h) or left untreated(NT). After the application time, patches were removed and wounds were allowed to heal spontaneously. The rate of wound closure was evaluated by digital analysis of unclosed wound area in course of time. At day 10, wounds(n=7 per group) were harvested and quantified with immunohistochemical markers of proliferation(Ki-67) and vascularization (platelet endothelial cell adhesion molecule, PECAM-1). Results: Wounds dressed with PGlcNAc patches for 1 hour closed faster than control wounds, reaching 90% closure in 16.6 days, nine days faster than untreated wounds. Granulation tissue showed higher levels of proliferation and vascularization following 1 h treatment than the 24 h and NT groups. In addition to its hemostatic properties, the PGlcNAc material also appears to accelerate wound closure in healing-impaired genetically diabetic mice. Conclusion: This material, with its combination of hemostatic and wound healing properties, has the potential to be effective agent for the treatment of complicated wounds.

• 동의생리병리학회지
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• 제22권5호
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• pp.1240-1249
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• 2008

Kamigingansikpung-tang(KGST) has been used for many years as a therapeutic agent for acute stage of cerebrovascular disease and hypertension in oriental medicine. But the effect of KGST on hypertension and vascular system is not well-known. This study was done to investigate the effects of KGST on hypertension. The results were obtained as follow: KGST showed scavenging activity on DPPH free radical. KGST showed the inhibitory effect on ROS and ACE, and the accelerated SOD activity. KGST significantly decreased the blood pressure and pulse in DOCA-salt induced hypertensive rat. KGST significantly decreased the levels of aldosterone in DOCA-salt induced hypertensive rat. KGST significantly decreased the levels of dopamine, epinephrine in DOCA-salt induced hypertensive rat. KGST significantly decreased the levels of potassium(K+) and chloride(Cl-) in DOCA-salt induced hypertensive rat. KGST significantly decreased the levels of uric acid and creatine in DOCA-salt induced hypertensive rat. KGST has an effect on inhibiting cell damage of the heart, liver, kidney, and adrenal gland. results suggest that KGST might be effective in treatment and prevention of hypertension.

• Biomolecules & Therapeutics
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• 제21권4호
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• pp.313-322
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• 2013

Previous studies have demonstrated that repeated administration of the exogenous stress hormone corticosterone (CORT) induces dysregulation in the hypothalamic-pituitary-adrenal (HPA) axis and results in depression and anxiety. The current study sought to verify the impact of catechin (CTN) administration on chronic CORT-induced behavioral alterations using the forced swimming test (FST) and the elevated plus maze (EPM) test. Additionally, the effects of CTN on central noradrenergic systems were examined by observing changes in neuronal tyrosine hydroxylase (TH) immunoreactivity in rat brains. Male rats received 10, 20, or 40 mg/kg CTN (i.p.) 1 h prior to a daily injection of CORT for 21 consecutive days. The activation of the HPA axis in response to the repeated CORT injections was confirmed by measuring serum levels of CORT and the expression of corticotrophin-releasing factor (CRF) in the hypothalamus. Daily CTN administration significantly decreased immobility in the FST, increased open-arm exploration in the EPM test, and significantly blocked increases of TH expression in the locus coeruleus (LC). It also significantly enhanced the total number of line crossing in the open-field test (OFT), while individual differences in locomotor activities between experimental groups were not observed in the OFT. Taken together, these findings indicate that the administration of CTN prior to high-dose exogenous CORT significantly improves helpless behaviors, possibly by modulating the central noradrenergic system in rats. Therefore, CTN may be a useful agent for the treatment or alleviation of the complex symptoms associated with depression and anxiety disorders.

• 정보와 통신
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• 제11권6호
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• pp.37-54
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• 1994

멀티미디어 기술의 획기적 발전으로 이를 응용한 다양한 서비스가 개발되고 있다. 본 논문에서는 멀티미디어 응용 서비스를 TV, 컴퓨터, 통신, 주변기기, 소프트웨어 계열로 분류하고 설명하였다. 이중 컴퓨터 부분에서 멀티미디어 서비스를 중점적으로 설명하며 이러한 서비스가 제공되기 위한 시스템의 주요 서브시스템으로서 멀티미디어 데이터 처리를 위한 초고속 통신망, 운영체제, 지능형 에이젼트에 대한 논의를 하였다. 이중 특히 멀티미디어 운영체제 기술은 종합 멀티미디어 서비스 제공을 위한 하부 구조로서 최근 가장 활발히 발전되고 잇는 분야이다. 이에 본 논문에서는 일반적인 멀티미디어 운영체제의 발전방향 및 현재 개발 완료된 COSMOS 멀티미디어 그룹표현용 운영체제에 관한 설명을 한다. COSMOS의 특성, 모델, 추상적 데이터 구조에 관한 논의와 더불어 COSMOS상에서 개발된 3자간 영상회의시스템의 성능분석을 통하여 COSMOS와 같은 운영체제를 통한 멀티미디어 운영체제의 제공이 멀티미디어 서비스 환경변화를 주도함을 설명하였다.