• Journal of fish pathology
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• v.34 no.1
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• pp.9-15
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• 2021

Rock bream (Oplegnathus fasciatus) is a highly valued aquaculture species in Korea. However, the aquaculture industry suffers huge economic losses due to rock bream iridovirus (RBIV) infection in summer. The objective of this study was to determine genetic diversity and relationships of DNAs isolated from two groups of rock bream after RBIV infection using five microsatellite (MS) markers. The first group of fish died early and the second group of fish died later after RBIV infection. In this experiment, 90 fish (5.1±1.0 cm and 4.1±1.3 g) were injected with 50 μl of RBIV (10⁴ TCID⁵⁰/ml) and maintained at 26℃ for 15 days. Genomic DNAs were extracted from fins of 20 fish that died earlier or later after RBIV infection. These DNAs were subjected to genotyping using five MS markers (CA-03, CA3-05, CA3-06, CA-10, and CA3-36). Of these markers, CA3-05 (early death group), CA3-06 (late death group), and CA3-36 (both early and late death groups) showed different alleles distribution rates. In-depth studies are needed to provide valuable information for selecting RBIV-resistant fish. In conclusion, microsatellite marker distribution pattern differences between early- and late- death groups of rock bream after RBIV infection showing different RBIV susceptibilities were determined using MS markers and genotyping. Results of this study suggest that MS markers could be used to facilitate the selection of RBIV resistant rock bream.

• Korean Journal of Veterinary Research
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• v.20 no.2
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• pp.93-97
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• 1980

To observe sequential postmortem autolysis in liver of Korean native cattle, liver placed in controlled environment chamber at $23-27.5^{\circ}C$ was treated after various intervals and observed with light microscope. The nuclear fading accompanied by margination of the chromatin in the hepatocytes appeared by 3 to 6 hours, and reduction of the cytoplasmic stainability and haryolisis began at 15 hours after death. Pyknosis of Kupffer's cells was noted by 3 to 20 hours, thereafter followed the decrease in staining of nuclei, but the nuclei were visible until 50 hours after death. Sinusoids and space of Disse were markedly distended at 10 hours, thereafter spaces of Disse were more dilatable than sinusoids. A large number of the erythrocytes in blood vessels were lysed, and the cytoplasmic stainability was largely reduced at 25 hours and nearly disappeared at 40 hours after death.

• Food Science and Biotechnology
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• v.15 no.4
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• pp.534-542
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• 2006

The effects of ethanol fractions of three different rice grain extracts, Jakwangchalbyeo, Hwasunchalbyeo, and Ilpumbyeo, on apoptotic cell death in the rat hepatoma H4IIE cell line were investigated using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cell viability assay. One hundred mg/mL Jakwangchalbyeo extract significantly reduced cell viability to 69.5, 57.2, and 46.1% within 24, 48, and 72 hr, respectively. Fluorescence-activated cell sorting (FACS) analyses were also performed to characterize the cell death pattern caused by treatment with the rice grain extracts. Apoptotic cell death was clearly observed with time after treatment with the Jakwangchalbyeo extract. In Western blotting analysis, degradation of the 116 kDa poly-ADP-ribose polymerase (PARP) molecule was observed with concomitant formation of an 89 kDa product 24, 48, and 72 hr after treating cells with the Jakwangchalbyeo extract. This indicates that an apoptotic process caused cell death in these cells. In conclusion, red-pericarp Jakwangchalbyeo extract induced apoptotic cell death in H4IIE cells to a larger extent than the other rice extracts.

• The Korean Journal of Health Service Management
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• v.5 no.1
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• pp.159-170
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• 2011

This study aims at providing basic data on brain death by analyzing factors of influencing toward attitude to brain death subject to citizens of Busan City. The data was collected for 47 days from 14 July to 31 August, 2009. Among a total of 2200 cases of the questionaries, only 2042 cases were used. For data analysis, SPSS 17.0 was used, and for the specific analysis method frequency analysis to understand general characteristics of the participants. In addition, examination on T-test and ANOVA analysis were conducted after analyzing the factors for participants' consciousness on brain death, and logistic regression analysis for understanding of relations between participants' will to brain death and general characteristics. The results of this study are summarized as follows; First, attitudes towards brain death according to general characteristics was high in those with will to donate their organs than those without in the attitude factors, namely, death recognition, acceptive, exclusive and religious attitude factors. Second, Significant variables for effects of attitudes towards brain death were gender, patients or their family's chronic or incurable diseases, religion, occupation and death recognition, acceptive, and exclusive attitude factors.

• Journal of English Language & Literature
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• v.58 no.4
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• pp.611-632
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• 2012

Beckett's life-long struggling with death may be illuminated in terms of the Western tradition of thanatology as well as Philippe Ariès's anthropological classification of death. Among the Western tradition, Beckett's oeuvre incarnates memento mori, timor mori, nihilism, theatrum mundi, life as afterlife, and the transsubstantiation of the self. Among the five views of death Ariès suggests, Beckett appears to foreground the death of the self and the invisible dirty death. In a world devoid of transcendental Signified, Beckett's resident is "a poor player/That struts and frets his hour upon the stage." Our contemporary vision of death is dominated by the dirty death and timor mori resurrected from the cultural icon of danse macabre in the late Mediaeval age as vividly dramatized in W;t by Margaret Edson. Beckett stands in no man's land: Lucky complains of divine aphathia as well as scopes at the possibility of God's existence like Hamm. Beckett's way of getting out of the dilemma is laughing a mirthless and dianoetic laugher. To bourgeois class who shudder at the sight of Grim Death after forgettable years of indulgence and addiction to capitalist consumption, Beckett seems to preach, your life is a death-in-life, you are not born yet until you are baptized with existential awakening as Gregor Samsa in Kafka's Verwandlung, or Tolstoy in Confession.

• Human Ecology Research
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• v.52 no.4
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• pp.325-336
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• 2014

The purpose of this study is to investigate the relationship between self-esteem and the view of the afterlife of the elderly by focusing on mediating effects of positive satisfaction and death preparation. Therefore, this study emphasizes the importance of the rest of life and contributes as exploratory research toward strengthening the psychological and emotional well-being of the elderly. After using a structural equation model, we found that self-esteem influenced their view of the afterlife through mediating effects such as positive satisfaction and the level of death preparation. The results of this study will present not only in recovering from death anxiety through an understanding of the level of death preparation and, the view of the afterlife but also in the development of practical intervention materials, which can be useful in social work for the elderly. The elderly's view of the afterlife should be strengthened according to the educational programs on death preparation that deal with the existence of the afterlife and life after death. However, this study has the limitation that only people living in Seoul and Gyeonggi Province and not in the other areas of the country were considered in this research. Nevertheless, within its limitation, this study's findings are promising, as they contribute to our understanding of the view of life and death of the elderly; further, the study has academic meaning with respect to helping the elderly successfully carry out their life development tasks and significantly prepare for the rest of their life.

• Anxiety and mood
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• v.16 no.1
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• pp.1-8
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• 2020

Objective : This study aims to examine the association between exposure to suicide events and suicide ideation by analyzing the levels of suicide ideation among the groups with exposure to suicide death, non-suicide death, and no death in their social relationships. Methods : Data were derived from Wave I of the Longitudinal Study of Suicide Survivors' Mental Health. 1,998 adults nationwide selected using a stratified sampling method based on the Korean Census Data, were categorized into 3 groups with exposure to suicidal death, non-suicidal death, and no death. The levels of depression (Brief CES-D), subjective health status, and suicidal ideation (SSI) were measured. To examine the association between exposure to suicide and the level of suicide ideation, multiple regression analysis was used after controlling the socio-demographic and clinical factors including subjective health status and depression. Results : 32% reported their exposure to suicide. Compared to the other groups, the suicide-exposed group's level of depression and suicide ideation were significantly higher but the subjective health status was lower. Multiple regression model revealed that suicide exposure had a statistically significant association with suicidal ideation at p=0.000 even after controlling the clinical characteristics. Conclusion : HThe findings suggest that exposure to suicide is a risk factor for suicidal ideation. In the clinical field, it is necessary to consider patients' experience in exposure to suicide while treating and intervening in suicide-related cases. At the policy level, a mental health system for suicide prevention should consider this risk factor for those exposed to suicide in their family and social relationships.

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.3
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• pp.227-227
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• 2022

• Journal of Hospice and Palliative Care
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• v.20 no.2
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• pp.100-110
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• 2017

Purpose: We explored Koreans' perception of the meaning of death with dignity that Korean people. Methods: A phenomenological research methodology was applied. A total of 13 participants were sampled based on their age and gender. Participants were interviewed in depth from September 2015 through February 2016. Colaizzi's phenomenological analysis method was used for data analysis. To establish the validity of the study, we evaluated its realistic value, applicability, consistency and neutrality of the qualitative evaluation criteria of Lincoln and Guba. Results: Koreans' perception of death with dignity was structured as 19 themes, nine theme clusters and four categories. The four categories were "comfortable death", "good death", "resolving problems before death", and "death with good reputation". The theme clusters were "death without pain", "death submitting to one's fate", "death that is not ugly", "leaving good memories to others", "dying in a way we want", "death after proper settling of things", "dealing with chronic resentment before death", "death after living a good life", and "death with recognition". Conclusion: For Koreans, death with dignity meant not burdening others, settling things right and leaving good memories to their families and friends. Such perceptions can be applied to hospice care for terminally ill patients.

• Anatomy & Biological Anthropology
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• v.31 no.4
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• pp.159-165
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• 2018

The aim of this study is to identify changes of recognition to death before and after observation on the cadaver dissection to paramedical students. This study was done on 472 freshmen using questionnaire survey. Recognition to death questionnaire was consisted of 36 items. Data were collected before and after observation on the cadaver dissection with agreement of subjects. The data were analyzed using SPSS win 24.0. This study showed that recognition to death was significantly increased after observation on the cadaver dissection (3.19 points) than before observation on the cadaver dissection (3.06 points) (p=.000). Especially, anxiety on death was significantly increased (p=.000), and interest in death was significantly increased, too (p=.000). The results of this study suggest that we need positively to encourage observation on the cadaver dissection for paramedical students with providing program to decrease anxiety on death because of not only improving anatomy knowledge but also increasing recognition to death.