• 제목/요약/키워드: Aflatoxin

검색결과 483건 처리시간 0.023초

Aflatoxin이 Bacillus megaterium의 생육(生育)에 미치는 영향 (Effect of Aflatoxin on the Growth of Bacillus megaterium)

  • 최언호;이관영;이서래
    • 한국식품과학회지
    • /
    • 제8권1호
    • /
    • pp.47-52
    • /
    • 1976
  • Aflatoxin이 함유된 TGY 액체배지(培地)에서 Bacillus megaterium NRRL B-1368 균주를 배양시 공시균(供試菌)의 생육저해(生育沮害), 형태적(形態的) 변화 및 정상(正常)배지에서의 생육회복(生育回復)과정을 조사하였다. Crude aflatoxin $(B_1\;22.7%,\;B_2\;1.6%,\;G_1\;3.6%,\;G_2\;0.2%)$의 농도 $20\;{\mu}g/ml$ 이상에서는 공시균(供試菌)의 생육(生育)이 완전히 억제(抑制)되었고 격막(隔膜)이 형성되지 않아 기형적(畸形的)으로 신장(伸長)하는 세포분렬의 장해현상을 보였다. 이들 기형(畸形)세포를 정상(正常)배지에서 다시 배양하면 격막이 형성되면서 정상(正常)세포로 분렬, 증식되었다. 따라서 aflatoxin은 세균의 격막형성에 관계하는 mesosome의 기능(機能)에 영향을 미치는 것으로 추론(推論)되었다.

  • PDF

Study on Applying Artichoke Extract to Lessen The Toxicity of Aflatoxin to Chicken

  • Diep, Le Thi Ngoc
    • Toxicological Research
    • /
    • 제17권
    • /
    • pp.281-287
    • /
    • 2001
  • The Artichoke extract at 10% was used to add in drinking water to understand its effect on Aflatoxicosis of chickens. The Artichoke extract at the dose of 6 ml per liter of drinking water was given (experiment group) or not (control group) and to Hybro chickens (150 birds), during the first 49 days of life. Also, the chickens were fed with foodstuff containing 200 ppb or 500 ppb Aflatoxin $B_1$. Results showed that, the chickens having Artichoke extract: (1) Had overcome the growth retardation caused by the toxin at concentration of 200 ppb and 500 ppb of Aflatoxin $B_1$ (an addittonal weight gain of about 200-400 g/bird). (2) The feed conversion was improved (a reduction of 200-400 g of feed per kg of bird living weight). (3) Aflatoxicosis lesions were mild in the chickens, which fed 500 ppb of Aflatoxin $B_1$ or not found in those having the toxin 200 ppb. The blood examinations at 28th and 49th days of the trial gave the following results: (1) The Artichoke extract had an effect of suppressing the changes of blood cell numbers, hemoglobin amount. packed cell volume. leukocyte formula that were caused by Aflatoxin $B_1$. (2) The Artichoke extract had an effect of suppressing the diminution oj sugar, protein levels and the increase of the levels of GOT, GPT, alkaline phosphatase and bilirubin in the blood of intoxicated chickens. There was not or very Jew residue of Aflatoxin $B_1$ contained in the liver and muscle of chickens intoxicated by Aflatoxin $B_1$ having Artichoke, that was much lower than the allowed level in animal products.

  • PDF

TLC법에 의한 장류 및 공류중의 Aflatoxin검출에 관한 연구 (The Study on the Detection of Aflatoxins in the Fermentation Products and Cereals)

  • 한양일;김광호;오영복
    • 한국환경보건학회지
    • /
    • 제5권1호
    • /
    • pp.46-50
    • /
    • 1978
  • Aflatoxin, a mixture of the at least four toxic and carcinogenic metabolites, is known to be produced by only a few fungi. The toxins were designated aflatoxins because they were produced by the mold Aspergillus flavus(A. flavus). However, at least four other toxins and other species of the genus A. niger, A. parasiticus A. ruber and wentii have been reported to produce aflatoxins. And also the identical compounds may also be produced by molds, the Pencillium. At least four different species of Penicilliurn have been reported to produce aflatoxins (P. citrinurn, P. frequentans, P. puberulurn. and P. variable). So it is now known that the problem of Aflatoxin is not restricted to the single species A. flavus, even though that is a very common mold. Also additional aflatoxins have been discorvered. For sereral years, only four aflatoxins were known: $B_1, B_2, G_1$ and $G_2$, so designated by reason of their fluorescence and chromatographic charateristics. It is now known that there are really two new toxic materials in the milk. During the past year(1966) they were christened aflatoxin $M_1$ and $M_2$, since they were first found in milk. The two other and most recently discorvered aflatoxins were isolated late in 1966 from cultures of A. flavus, and were designated aflatoxin $B_2a$ and aflatoxin $G_2a$. In order to obtain a breaf information about extent of contamination of foodstuffs by aflatoxin which is known to produce eight different mold, aflatoxin detection of cereals and fermented foods on sale, such as polished rice, barley, wheat, wheat flour, lentil, red bean, soy bean, noodle, kochuj ang and Dwenjang (fermented soy bean paste) and chong Kuk, were carried out. The results of this investigation were summarized as follows: The hexane:$CHCl_3$ extracts of polished rice, barley wheat, wheat flour, lentil, red bean, noodle and kochujang yielded fluorescent spots on thin layer plates. However their Rfvalues were different from those of authentic aflatoxins. The fluorescent substances of the extract from soy bean, Dwenjang and chong kuk showed very similar Rf values to those of the standard aflatoxins. By two dimensional thin layer chromatography and comparison of ultra violet absorption spectra, it was found that these fluorescent substances were not aflatoxins. To conclude, aflatoxins themselves were not detected directly in those samples tested.

  • PDF

우유 중 아플라톡신 M1 오염도 조사연구 (Analysis and Monitoring of Aflatoxin M1 in Milks)

  • 박성국;강영운;권기성;이광호;김미혜
    • 한국식품과학회지
    • /
    • 제44권2호
    • /
    • pp.247-250
    • /
    • 2012
  • 본 연구에서 적용한 시험법의 회수율은 97.4-122.5%, 상대표준편차(RSD)는 6%이내로서 적합한 시험법임을 확인하였다. 조사된 원유 및 시유의 검출농도는 아플라톡신 $M_1$이 평균 29.6 ng/kg(5.4-72.7 ng/kg)로 나타났으며, 원유 및 시유에 대한 아플라톡신 $M_1$ 오염수준은 현행 식품공전에서 정한 허용기준치인 500 ng/kg 보다 낮은 안전한 수준인 것으로 판단된다.

Effect of Aflatoxin on Feed Conversion Ratio in Broilers: A Meta-analysis

  • Suganthi, R. Umaya;Suresh, K.P.;Parvatham, R.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제24권12호
    • /
    • pp.1757-1762
    • /
    • 2011
  • Aflatoxins are natural contaminants of poultry feeds and feed ingredients and cause liver damage, immunosuppression, reduction in performance and mortality in broilers. A number of studies have been carried out to study the effects of aflatoxin on feed conversion ratio in broilers. The results on feed conversion ratio of 10 research articles in broilers fed with aflatoxin from first day of age to six weeks of age were compiled and were subjected to meta-analysis. Chi-square test and $Tau^2$ (heterogeneity co-efficient) were applied to test for significance of heterogeneity of studies. To integrate results, fixed effect model by Inverse Variance method (IV method) was used when heterogeneity was insignificant and otherwise random effect model by DerSimonian and Laird Method (DL method) was used. The results of meta-analysis showed that the adverse effect of aflatoxin on feed conversion ratio at the end of first week was negligible, second week was medium and third to six weeks was very large.

Aspergillus flavus와 Bacillus subtilis의 혼합배양에 의한 Aflatoxin생성에 관하여 (Study on the Productivity of Aflatoxin through the Interaction of Bacillus subtilis & Aspergillus flavus)

  • 서명자
    • 미생물학회지
    • /
    • 제17권2호
    • /
    • pp.72-80
    • /
    • 1979
  • 24 samples which were incoulated with Aspergillus flavus and Bcillus subtilis and cultured on the steamed soybean media under various conditions-pH, moisture and temperature were-investigated on the production of aflatoxin by the interaction of these two microorganisms. 1) The amount of aflatoxin produced by mixed cultures of Aspergillus flavus and Bacillus subtilis was decreased significantly rather than that of single cultures of Aspergillus flavus. 2) Maximum production of crude aflatoxin was 2,560 ppm $(B_1,\;0.908\;ppm;\;B_2,\;0.261\;ppm;\;G_1,\;1.162\;ppm;\;G_2,\;0.229\;ppm)$ at 30% moisture, pH 5.0 and $20^{\circ}C$, whereas minimum production was 1.107 ppm $(B_1,\;0.341\;ppm;\;B_2,\;0.104\;ppm;\;G_1,\;532\;ppm;\;G_2,\;0.130\;ppm)$ at 63% moisture, pH 9.0 and $40^{\circ}C$.

  • PDF

Control of Aflatoxin Production of Aspergillus flavus by Inghbitory Action of Antagonistic Bacteria

  • Cho, Jung-Il;Hong, Kwang-Won;Kang, Kil-Jin
    • Journal of Microbiology and Biotechnology
    • /
    • 제10권2호
    • /
    • pp.154-160
    • /
    • 2000
  • Aflatoxin B1 is known as the most potent mycotoxin produced by several fungi. It has been demonstrated to be not only carcinogenic but teratogenic and mutagenic as well in humans. To prevent or inactivate aflatoxins, several chemical of physical methods were tested for ammoniation, using insecticides as an wxample, but they were unsuitable for food products. On the contrary, biological control by antagonistic microorgani는 is and ideal method. In order to control aflatoxin B1 biologically, the antagonists #07, #63, #75, #74, and #61 were separated from various samples by using the antagonistic activity test. Among them, culture filtrate part A (non heat-treated) of #63 and #74 on aflatoxin B1 produced by Aspergillus fkavus were shown to be 95% and 75%, respectively. Based on the morphological characteristics, #63 was deduced as an Azospirillum sp.

  • PDF

STUDY ON AFLATOXINS IN SOME FOODSTUFFS WITH SPECIAL REFERENCE TO PUBLIC HEALTH HAZARD IN EGYPT

  • EL-GOHARY, A.H.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제8권6호
    • /
    • pp.571-575
    • /
    • 1995
  • A total of 455 food samples representing 22 different food types were collected from several localities at Alexandria province in Egypt. Aflatoxin B1 and aflatoxin M1 were detected in 5 out of 455 (1.1%) of these food samples. From the same samples 206 fungal isolates were obtained. Thirty two of these isolates (15.5%) were found to be aflatoxin producers. Aspergillus flavus was the dominant isolate, while Aspergillus parasaticus was also isolated from a few other food samples. Among locally consumed foodstuffs. Peanut (7.5%) and Milk powder (6.6%) were found to be a suitable substrates for aflatoxin production. The hygienic and public health significance of the isolated aflatoxigenic strains were discussed.

Aspergillus flavus에 의한 Aflatoxin 생산능(生産能)에 Steroidal Carbamate Derivatives가 미치는 영향(影響) (Effects of Steroidal Carbamate Derivatives on the Aflatoxin Productivity by Aspergillus flavus)

  • 정승재;서명자
    • 한국균학회지
    • /
    • 제13권4호
    • /
    • pp.243-247
    • /
    • 1985
  • Experiments were conducted to study effects of steroidal carbamate derivatives upon mycelial growth and aflatoxin production by Aspergillus flavus ATCC 15517. The basal medium was supplemented with various concentrations of these compounds and inoculated with spores. The developing cultures were incubated for 11 days at $28^{\circ}C$ without agitation. Aflatoxins were extracted with chloroform, separated by thin layer chromatography, and quantitated by ultraviolet spectrophotometry. At a concentration of 50 mg per 30 ml of medium., stigmasteryl-N-(2-chloroethyl) carbamate, cholesteryl- N - (2-chloroethyl) carbamate, $5{\alpha}-cholestan-3-one-oximino-N-(2-chloroethyl)$ carbamate and ${\beta}-sitosteryl-N-(2-chloroethyl)$ carbamate were the most effective in reducing aflatoxin production by Aspergillus flavus. However, cholest-4-ene-3-one-oximino-N-(2-chloroethyl) carbamate, at a concentration of 100 mg per 30 ml, significantly decreased aflatoxin production. There was no significant inhibition of mycelial growth by the addition of the various concentrations of these compounds.

  • PDF

Aflatoxin-mediated Sperm and Blood Cell Abnormalities in Mice Fed with Contaminated Corn

  • Fapohunda, S. O.;Ezekiel, C. N.;Alabi, O. A.;Omole, A.;Chioma, S. O.
    • Mycobiology
    • /
    • 제36권4호
    • /
    • pp.255-259
    • /
    • 2008
  • The effect of aflatoxin-contaminated corn on albino mice was investigated using the sperm morphology assay. Blood parameter levels including; total white blood cells (WBC), total red blood cells (RBC), packed cell volume (PCV), serum bilirubin (SB) and fasting blood sugar (FBS) were also determined in the tested mice. Test mice were exposed to aflatoxin-contaminated corn (contamination level of 100 ppb) for $1{\sim}4$ weeks while aflatoxin-free corn and cyclophosphamide were used as negative and positive controls, respectively. Sperm cells showed varieties of morphological abnormality when assessed after 5 weeks. The percentage frequencies of the negative and positive controls were 18.8% and 48.87%, respectively, while the percentage abnormalities for the 1, 2, 3 and 4 weeks exposures were 41.38%, 48.17%, 57.13% and 61.67%, respectively. PCV, WBC, total bilirubin and glucose level values of mice in all concentrations were higher and statistically significant as compared to the negative control values using Dunnett's test. Therefore, abnormal sperm cell induction is concentrationdependent such that continuous consumption of aflatoxin-contaminated corn is capable of negatively affecting spermatogenesis by inducing or increasing the frequency of morphologically abnormal sperm cells produced.