• Title/Summary/Keyword: Aerobic phase

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Global Regulation of Gene Expression in the Human Gastric Pathogen Helicobacter pylori in Response to Aerobic Oxygen Tension Under a High Carbon Dioxide Level

  • Park, Shin Ae;Lee, Na Gyong
    • Journal of Microbiology and Biotechnology
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    • v.23 no.4
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    • pp.451-458
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    • 2013
  • The human gastric pathogen Helicobacter pylori (Hp) has been considered a microaerophile. However, we recently reported that, when supplied with 10% $CO_2$, Hp growth is stimulated by an atmospheric level of $O_2$, suggesting that Hp is a capnophilic aerobe. In this study, we investigated the effects of aerobic $O_2$ tension on Hp cells by comparing gene expression profiles of cultures grown under microaerobic and aerobic conditions in the presence of 10% $CO_2$. The results showed that overall differences in gene expression in Hp cells grown under the two $O_2$ conditions were predominantly growth-phase-dependent. At 6 h, numerous genes were down-regulated under the aerobic condition, accounting for our previous observation that Hp growth was retarded under this condition. At 36 h, however, diverse groups of genes involved in energy metabolism, cellular processes, transport, and cell envelope synthesis were highly up- or down-regulated under the aerobic condition, indicating a progression of the cultures from the log phase to the stationary phase. The expression of several oxidative stress-associated genes including tagD, katA, and rocF was induced in response to aerobic $O_2$ level, whereas trxA, trxB, and ahpC remained unchanged. Altogether, these data demonstrate that aerobic $O_2$ tension is not detrimental to Hp cells but stimulates Hp growth, supporting our previous finding that Hp may be an aerobic bacterium that requires a high $CO_2$ level for its growth.

Simplification of ASM No. 1 Using Aerobic-Anoxic SBR (호기-무산소 SBR 반응조를 이용한 ASM No. 1 모델의 간략화)

  • Kim, Shin Geol;Choi, In Su;Koo, Ja Yong
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.4
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    • pp.409-420
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    • 2007
  • ASM No. 1 is a very useful model to analyze wastewater treatment system removing organic carbon and nitrogen material. But it isn't adequate to control the wastewater treatment system with real time since it has many material divisions and parameters. So, the purpose of this study is the simplification of ASM No. 1 to control the wastewater treatment system. ASM No. 1 was changed with the model which has 3 material divisions(COD, $NH_4{^+}$, $NO_3{^-}$) and two phases(Aerobic and Anoxic condition). SBR was running with two phases(Phase I and II). Phase II running 20 minutes with aerobic time was used for deciding model parameters and Phase I running 12 minutes with aerobic time was used for proving the simplified model. The simplified model was compared with ASM No. 1 using data of Phase I and II. As a result of model comparison, the simplified model has enough ability to express the variation of $NH_4{^+}$ compound.

The evaluation of T-P removal and dewaterability under the operation change in KIDEA process (-기술정보- 연속유입 KIDEA에서 공정변화에 따른 인제거 및 탈수 함수율 상관관계)

  • Yeon, seung jun;Her, hee seung
    • Journal of Korean Society of Water and Wastewater
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    • v.22 no.2
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    • pp.179-182
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    • 2008
  • The KIDEA process, occurred in single reactor, is operated by three consequential steps, i.e., aerobic, settling, and discharge while introducing wastewater into the bottom of reactor continuously. It could accomplish biological oxidation (BOD), nitrification, denitrification (T-N), phosphate removal (T-P), and solid separation (SS) through the operational mode mentioned. Especially, this system has removed the T-P by wasting certain amount of sludge at the end of aeration phase during 5~10 minutes and not returned the activated sludge into the reactor, that is, no RAS (Return Activated Sludge). All running mode and instrumentation were controlled by the PLC equipment automatically. In this study, therefore, we have evaluated T-P removal efficiency and moisture content (MC) performance under the different excess sludge wasting mode. T-P track study and MC with TS concentration were analyzed during aerobic and settling phase. It has revealed that there was no significant difference of released T-P concentration between the first case which waste the sludge at the end of aerobic phase (0.2mg/L) and the second case which waste the sludge at 40 min of settling phase (0.25mg/L). Also, dewatering duration and MC have decreased 1.7% when TS concentration was increased from 0.31% to 0.5% during aerobic condition. Hence, it has concluded the system performance was less influenced by the operation time change of PLC program.

Removal of Phosphorus in Aerobic Fixed Biofilm Reactor (호기성 고정생물막 반응조에서 인의 제거)

  • Rim, Jay-Myoung;Han, Dong-Joon;Woo, Young-Gug
    • Journal of Industrial Technology
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    • v.16
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    • pp.5-11
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    • 1996
  • While the enhanced biological phosphorus removal(EBPR), in anaerobic/aerobic condition, was known to remove phosphorus by means of metabolism of poly-P microorganisms, the phosphorus removed could be released in the form of ortho-P in the aerobic fixed biofilm reactor. This study was initiated to investigate the cause of ortho-P release in the aerobic fixed biofilm reactor. The resutls indicated that the phosphorus release was caused by autooxidation. The synthesis and release of phosphrous were related to the ORP and the boundary value for the phase change was about 170mV. In the synthesis phase, the phosphorus removal rate per COD removed was $0.023mgP_{syn}/mgCOD_{rem}$. The phosprous contents of the microorganism were 4.3 ~ 6.0% on a dry weight basis.

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Analysis of Characteristics of Coupled Phosphorus in the Sewage (하수 중 인의 결합 특성 분석)

  • Choi, Hee-Jeong;Lee, Seung-Mok
    • Journal of Korean Society on Water Environment
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    • v.23 no.1
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    • pp.97-102
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    • 2007
  • The present investigation deals to achieve an accurate determination of the phosphorous present in the wastewater samples using the membrane reactor. The study may enable to quantify the dissolved (DP) and adsorbed phosphorous (AP), also the adsorbed phosphorous categorically identified as inorganic coupled phosphorous (DRP) and organic coupled phosphorous (NRP). Moreover, the study has been conducted separately in anaerobic and aerobic chamber. The results obtained showed that dissolved phosphorous only can occur in anaerobic chamber with ca. 25%. The study conducted for adsorbed phosphorous showed that the DRP has the percent composition in anaerobic and aerobic chamber respectively 33% and 40% i.e., 7% more in aerobic chamber. The similar values obtained for NRP was found to be 42% and 60% i.e., 18% more in aerobic chamber. On the other hand while comparing the results for NRP and DRP, it has to be noted that NRP has 9% and 20% more percent composition than DRP respectively in anaerobic and aerobic chamber. Further, the adsorbed phase showed the species Al-P, Fe-P in the aerobic chamber with the quotient of 7.73 mg/g TS (total solid) whereas in the anaerobic chamber it showed the species Fe-P and $Fe(OH)_3$-P with the 7.16 mg/g TS.

Microbiological Evaluations of Refrigerated Chicken wings Treated with Acetic Acid (초산으로 처리한 냉장 닭고기 날개의 미생물 평가)

  • 김창렬;이재일;김광현;문승주;이용규
    • Journal of Food Hygiene and Safety
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    • v.12 no.4
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    • pp.277-280
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    • 1997
  • The effects of acetic acid (AA) on aerobic plate counts (APC), gram-negative bacterial counts (GNC), and generation time (GT) in chicken wings stored at 4* were assessed. Chicken wings were treated with 0.5-1.5% (v/v) AA at exposure times of 5 min. Treatments of AA for 5 min significantly (P<0.05) reduced aerobic plate counts (APC) and gram-negative bacterial counts (GNC) on the surface of chicken wings for 8 days, respectively. After 4 days of storage, treatments of 1.0% AA and 1.5% AA for 5 min completely (P<0.05) inhibited APC and GNC compared to initial controls. Based on these results, treatments of 1.0% AA and 1.5% AA for 5 min prolonged the microbiological shelf-life for 8 days compared to those of 0.5% AA and the controls. All treatments of AA increased the lag phase and GT of aerobic microorganisma.

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Expression Analysis of phbC Coding for Poly-3-hydroxybutyrate (PHB) Synthase of Rhodobacter sphaeroides

  • Kho, Dhong-Hyo;Yang, Jai-Myung;Kim, Kun-Soo;Lee, Jeong-Kug
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.310-316
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    • 2001
  • Poly-3-hydroxybutyrate (PHB) synthase catalyzed the last enzymic step to synthesize the intracellular PHB of Rhodobacter sphaeroides. No PHB was detected when the phbC coding for PhB synthase was interrupted, and its expression was regulated at the level of transcription. The cellular PHB content increased about four- to six-fold during the growth transition from the exponential to the early stationary phase under both aerobic and photoheterotrophic conditions. The PHB content during the aerobic growth seemed to be determined by the PhB synthase activity. However, the PHB synthase activity of photoheterotrophically grown cells did not correlate with the PhB content, suggesting a photoheterotrophic regulation different from the aerobic control. Thus, the PHB content of R. sphaeroides was regulated at the transcription level only under aerobic conditions.

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Nitrification and Denitrification by Using a Sequencing Batch Reactor System (Sequencing Batch Reactor (SBR)를 이용한 질산화와 탈질산화)

  • PARK Jong-Ho;LEE Won-Ho;CHO Kyu-Seok;HWANG Gyu-Deok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.3
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    • pp.247-253
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    • 2003
  • Sequencing Batch Reactor (SBR) was operated under various experimental conditions to improve the efficiency of biological filters used for the treatment of recycled wastewater from aquaculture. This SBR system was operated for removing COD, ammonia and suspended solid that were the major pollutants in aquaculture wastewater. Aerobic and anoxic conditions after FILL mode were applied intermittently for effective removal of nitrogen. SETTLE and DRAW modes were followed by the complete aerobic and anoxic REACT mode. The total volume of the SBR was 75 liter, while the working volume in a cycle was 35 liters. When the final operating strategy of the SBR was FILL/REACT/SETTLE/DRAW of 0.5/10/1/0.5 hr. the removal efficiencies of TCODcr, $NH_{4}^{+}-N,$ and T-N were 94, 98, and $89\%,$ respectively.

A shell layer entrapping aerobic ammonia-oxidizing bacteria for autotrophic single-stage nitrogen removal

  • Bae, Hyokwan;Choi, Minkyu
    • Environmental Engineering Research
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    • v.24 no.3
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    • pp.376-381
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    • 2019
  • In this study, a poly(vinyl) alcohol/sodium alginate (PVA/SA) mixture was used to fabricate core-shell structured gel beads for autotrophic single-stage nitrogen removal (ASNR) using aerobic and anaerobic ammonia-oxidizing bacteria (AAOB and AnAOB, respectively). For stable ASNR process, the mechanical strength and oxygen penetration depth of the shell layer entrapping the AAOB are critical properties. The shell layer was constructed by an interfacial gelling reaction yielding thickness in the range of 2.01-3.63 mm, and a high PVA concentration of 12.5% resulted in the best mechanical strength of the shell layer. It was found that oxygen penetrated the shell layer at different depths depending on the PVA concentration, oxygen concentration in the bulk phase, and free ammonia concentration. The oxygen penetration depth was around $1,000{\mu}m$ when 8.0 mg/L dissolved oxygen was supplied from the bulk phase. This study reveals that the shell layer effectively protects the AnAOB from oxygen inhibition under the aerobic conditions because of the respiratory activity of the AAOB.

The Effect of Aerobic Rhythmical Exercise Program on Physical Fitness, Self-efficacy and Quality of Life in Elderly (유산소 리듬 운동프로그램이 노인의 체력, 자기효능감 및 삶의 질에 미치는 효과)

  • Kim Jong Hwa;Park Young Sook
    • Journal of Korean Public Health Nursing
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    • v.14 no.1
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    • pp.12-25
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    • 2000
  • The purpose of this study was to determine the effects of aerobic rhythmical exercise program on $VO_2$ max, leg strength, grip strength, flexibility, exercise self-efficacy and quality of life in elderly. This quai-experimental study was designed as a non-equivalent control group pretest-posttest study. 36 subjects, aged between 65 and 73 years who have normal cognition. sensory function and cerebellum function participated in this study. 19 experimental group subjects participated aerobic rhythmical exercise program. Aerobic rhythmical exercise program was developed on the basis of calisthenics, stretching, aerobic exercise, Korean traditional dance and music by the author. The program consisted of approximately 1 hours of exercise, 3 times a week for 9 weeks. During 1 hours workout, there were 15 minutes of warm-up dancing, 35 minutes of conditioning dance and 10 minutes of cool-down dancing. The intensity for the conditioning phase was between $40\%\;and\;60\%$ of age adiusted maximum heart rates. The $VO_2$ max, grip strength, leg strength, flexibility, exercise self-efficacy and quality of life were measured prior to and following the experimental treatment. Data were analyzed with $x^2-test$, t-test, mean, standard deviation. percentage of change and ANCOVA test using SAS program. Results were obtained as follows. 1. The $VO_2$ max of the experimental group were significantly lower than those of the control group following the aerobic rhythmical exercise program(F=7.76, p=0.008). 2. The grip strength of the experimental group were significantly lower than those of the control group following the aerobic rhythmical exercise program(F=4.61, p=0.039). 3. The leg strength of the experimental group were significantly lower than those of the control group following the aerobic rhythmical exercise program(F=9.29, p=0.000). 4. The was no significantly difference of flexibility between experimental and control group 5. The self-efficacy of the experimental group were significantly lower than those of the control group following the aerobic rhythmical exercise program(F=43.40, p=0.000). 6. The quality of life of the experimental group were significantly lower than those of the control group following the aerobic rhythmical exercise program(F=8.91. p=0.005). The results suggest that aerobic rhythmical exercise program can improve $VO_2$ max, grip strength. leg strength. exercise self-efficacy and quality of life elderly. Thus. aerobic rhythmical exercise program was a useful nursing intervention for elderly.

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