• 한국환경과학회지
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• 제14권10호
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• pp.955-963
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• 2005

From sludge of S municipal wastewater treatment plant in Busan, Korea, we isolated the denitrifier DN-9 which showed the ability of denitrification under aerobic conditionby the color change and gas formation in liquid culture with Giltay medium. The isolated strain was identified as Pseudomonas sp. DN-9 on the basis of the morphological, physiological, biochemical and nucleotide sequence analysis of l6S rRNA. The isolated strain, Pseudomonas sp. DN-9, has cytochrome $cd_1$, nirS of nitrite reductase. By the co-existance of additional ammonium and nitrate ion, the strain was not affected largely on growth in SL series broth. It seemed the result of denitrification. Although Pseudomonas sp. DN-9 has a good nitrate reduction activity under aerobic condition, the activity is less than Pseudomonas stutzeri in same cultivation condition. However, Escherichia coli had little the activity of aerobic denitrification and Pseudomonas putida showed lower activity of aerobic denitrification than Pseudomonas sp. DN-9 and Pseudomonas stutzeri in this study.

• 한국물환경학회지
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• 제29권6호
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• pp.782-789
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• 2013

Enhanced biological phosphorus removal (EBPR) behavior and microbial characteristics in the anaerobic-aerobic SBR (PAO SBR) and the anaerobic-anoxic SBR (DPAO SBR) were examined in this research. For 392 days of operation, both SBRs have exhibited a good EBPR (or denitrifying EBPR) performance. $P_{release}/P_{influent}$ ratio was highest in both reactors after the stabilization, while the efficiency of phosphorus removal was decreased since the sludge granulation has been visually observed within the reactor. The comparative analysis of Pyrosequencing-based microbial population between PAO and DPAO sludges showed indirectly that Dechloromonas spp. could utilize $O_2$ and $NO_3{^-}-N$ as an electron acceptor and Accumulibacter phosphatis use only $O_2$ in EBPR system. Also, we concluded that Thauera spp. as a denitrifier contribute significantly to the anoxic phosphorus removal in the DPAO system.

• 청정기술
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• 제12권2호
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• pp.67-77
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• 2006

본 연구에서 소규모 하수고도처리를 위한 이중슬러지(Dual sludge) $KNR^{(R)}$ (Kwon's nutrient removal) 시스템이 개발되었다. $KNR^{(R)}$ 시스템은 부유성장식 탈질미생물과 부착성장식 질산화미생물을 분리시킨 이중슬러지 공정으로 최초침전조, 혐기조, 무산소조, 농축조의 복합기능을 수행하는 UMBR (Upflow multi-layer bioreactor)과 펠렛형 담체가 충진된 호기성 담체조로 구성되어 있다. 소규모 하수처리시 본 개발공정의 안정성과 처리성능을 평가하기 위해 처리용량 $50m^3/d$ 규모의 파일럿 플랜트를 고도처리 공정으로 개선공사 중인 처리용량 $50m^3/d$ 규모의 실제 소규모 마을하수처리장에 적용하였다. UMBR과 담체조의 체류시간은 각각 4.7 h와 7.2h이었으며, 반응조 수온은 $18.1{\sim}28.1^{\circ}C$이었다. 유입 하수량과 유입수의 BOD/N의 변동폭이 컸음에도 불구하고 파일럿 플랜트는 안정된 처리성능을 보였다. 전체 실험기간 중 처리수의 $COD_{cr}$, $COD_{Mn}$, $BOD_5$, TN, TP의 평균 농도는 11.0 mg/L, 8.8 mg/L, 4.2 mg/L, 3.5 mg/L, 9.8 mg/L, 0.87/0.17 mg/L (poly aluminium chloride(PAC) 투입/미투입)이었으며, 제거율을 각각 95.3%, 87.6%, 96.3%, 96.5%, 68.2%, 55.4/90.3% 이었다. 잉여슬러지 발생량은 $A_2O$와 Bardenpho 등과 같은 단일슬러지를 이용하는 고도처리공정과 비교시 약 1.9~3.8배 낮은 $0.026kg-DS/m^3$ and 0.220 kg-DS/kg-BOD로 나타났다.