• 생약학회지
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• 제48권1호
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• pp.88-92
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• 2017

In the present study, we carried out quantitative analysis of chrysin and pinocembrin in Korean propolis by ultra performance liquid chromatography (UPLC) equipped with diode array detector. The separation was done using BEH C18 ($2.1{\times}50mm$, $1.7{\mu}m$) column with a mobile phase consisting of MeCN and 0.1% $H_3PO_4$ at 280 nm. The chromatographic method was validated for specificity, limit of detection, limit of quantification, linearity, precision, and accuracy. A quantitative analysis exhibited that the contents of the two compounds in Korean propolis collected from 8 inland areas except Jeju-do ranged from 3.1-46.0 mg/g. These results will be valuable as basic data for standardization of Korean propolis.

• 식물병연구
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• 제21권1호
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• pp.24-26
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• 2015

In April 2014, seedlings of Fallopia multiflora showing wilt symptom were first found at a greenhouse in Punggi-eup, Yeongju-si, Korea. A Fusarium-like fungus was isolated from the wilted plant and it was identified as Fusarium oxysporum based on morphological characteristics and nucleotide sequence data of translation elongation factor 1-${\alpha}$. The fungus isolated from the diseased plant was revealed to be pathogenic to the host plant through pathogenicity tests, and the reisolation of the pathogen confirmed Koch's postulates. This is the first report of Fusarium wilt occurring on Fallopia multiflora in the world.

• 한국직업건강간호학회지
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• 제25권4호
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• pp.320-328
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• 2016

Purpose: The purpose of this study was to compare the job stress, health promotion behaviors and quality of life between public health officials and general administration officials. Methods: Subjects were 60 public health officials and 71 general administration officials in G-City in Korea. Data were collected from January 25 to February 5, 2016 through self-administered questionnaires and analyzed t-test and Pearson's correlation coefficients. Results: Job stress (t=4.060 p<.001) and quality of life (t=3.963, p=.025) were significantly different between public health officials and general administration ones, while health promotion behaviors were not significantly different between these two official groups (t=0.394, p=.606) Conclusion: Results of this study suggested that it was necessary for public health officials to develop intervention program aimed at reducing job stress. Also, it would be effective to focus on improving health promotion behaviors and quality of life for them.

• Journal of Biosystems Engineering
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• 제41권3호
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• pp.170-176
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• 2016

Purpose: This study aimed to improve the method for determining the position of the center of gravity for agricultural tractors. Methods: The proposed method uses trigonometric functions and coordinate transformation. Data were measured according to the ISO 789-6 test procedures for the center of gravity of agricultural tractors. The height calculated using the proposed method was compared with that determined from an AutoCAD drawing. To find the center of gravity of the tractor, the algorithm for finding the intersection of the two lines was used. Results: The vertical height from the ground to the center of gravity is 682.06 mm. The vertical coordinates obtained from the calculation and the drawing were the same. Conclusions: The developed method uses trigonometric and polar coordinate transformation. The method was compared and verified with the AutoCAD drawing results. The results indicate that users can apply this developed method instead of the plotting method which is an inconvenient and time-consuming. Further, users can program Microsoft Excel to easily determine the vertical coordinate. In addition, researchers will propose this method to the ISO as a standard method for determining the center of gravity in accordance with ISO 789-6.

• 대한수의학회지
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• 제44권2호
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• pp.201-206
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• 2004

Norfloxacin (NFX) is a fluorquinolone antibacterial agent with a high antimicrobial activity and might have great potential for treating common infections in poultry. The objective of this study was to obtain comparative pharmacokinetic data after a single oral administration and medication with drinking water of norfloxacin-glycine acetate (NFX-GA) at the dose rate of 10 mg/kg bw in broilers. Fifty minutes following oral administration of NFX-GA, serum concentrations peaked at $1.32{\mu}g/mL$ (range $1.03-1.45{\mu}g/mL$). Serum concentration of NFX declined with a half-life of $7.21{\pm}1.81$ h. On the third day after administration of medicated drinking water, steady-state was reached, with mean concentrations of NFX of $0.70{\pm}0.35{\mu}g/mL$. The concentration of NFX after medication of NFX-GA with drinking for 3 days provides sufficient levels to obtain maximum therapeutic effects and maintains the serum persistence of concentration exceeding MIC.

• Molecules and Cells
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• 제26권3호
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• pp.319-322
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• 2008

Little is known about the chromosomal variability and polymorphism existing in mitotic chromosomes of Citrus, mainly due to lack of reliable chromosomal markers and small chromosome size. To test the hypothesis of chromosomal polymorphism and provide the foundation of the genome organization in the Citrus cultivars, we have developed molecular cytogenetic markers for 13 Citrus species collected from Jeju island, Korea. In this study, we demonstrated that the chromosomal locations of cytogenetic markers are quite variable and extremely polymorphic, in contrast to the previous studies. The data obtained in this study will be of utmost importance in cytological systematics and karyotyping of the Citrus species.

• 한국가정과학회지
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• 제1권1호
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• pp.94-102
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• 1998

This study was undertaken to investigate the effects of mustard leaf diet on the lipid metabolism under chronic alcohol administration. Sprague-Dawley male rats were fed either AIN-76 diet(control), control with ethanol, AIN-76 diet plus 5% mustard leaf(mustard leaf), or mustard leaf with ethanol lot 30 days. On the 21st day, all of the rats were given an oral dose of ethanol and blood-ethanol concentration were monitored for the next 5 hours. Lipid and enzyme determinations in serum and liver were carried out after 30 days. The results obtained were summarized as following: 1) Supplementing 5% of mustard leaf did not recover the body weight loss due to chronic alcohol administration. 2) There were no significant differences in blood ethanol concentrations among the experimental groups. 3) Mustard leaf diet decreased the plasma total cholesterol, triglyceride levels increased due to the chronic alcohol administration, but not HDL-, LDL-cholesterol, and liver lipids. 4) Mustard leaf diet decreased ${\gamma}$ -GTP level increased by chronic alcohol administration. Overall, these data suggest that mustard lear can have a recovery function, which was not via ethanol metabolism on the symptoms of alcohol related diseases.(Korean J Human Ecology 1(1) : 94~102, 1998)

• 기본간호학회지
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• 제9권3호
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• pp.370-378
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• 2002

Purpose: The purpose of this descriptive study was to investigate the administration patterns of sedatives and paralytic agents for mechanically ventilated patients. Method: The participants were 18 adult patients who received mechanical ventilation therapy for more than 1 day in the MICU. Collected data were type of injected medications, administration interval, injected amount, administration purpose and patient assessment by nurses. Result: Mean age of the patients was 61 years old. Seventy-two percent were male. Only 2 drugs (midazolam and norcuron) were administered to most of subjects. The type of medication administered was less heterogeneous than in previous studies. Most of the patients received sedatives and paralytics intermittently. Six different methods were used by nurses to administer sedatives and paralytics. The mean injection frequency was 9.3 times/day for sedatives and 10.8 times/day for paralytic agents. The most common purpose for administration of the drugs was full sedation of the patient (57%). Conclusion: Since a few patients received sedatives and paralytic agents higher than usual dose and a few Patients received those drugs lower than usual dose, nurses need to use proper protocols and guidelines for sedation to avoid oversedation and undersedation.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.556-561
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• 2010

Lactoferrin is an 80-kDa iron-binding glycoprotein that is found in high concentrations in human milk. Human lactoferrin (hLF) has several beneficial biological activities including immune system modulation and antimicrobial activity. In the present study, we devolved a method of hLF expression through introducing the hLF gene construct into Oriza sativa cv. Nakdong using the Agrobacterium-mediated transformation system. The expression of the hLF gene under the control of the rice glutelin promoter was detected in the seeds of transgenic rice plants. Transformed rice plants were selected on media containing herbicide(DL-phosphinothricin) and the integration of hLF cDNA was confirmed by Southern blot analysis. The expression of the full length hLF protein from the grains of transgenic rice plants was verified by Western blot analysis. The lactoferrin expression levels in the transformed rice grains determined by enzyme-linked immunosorbant assay accounted for approximately 1.5% of total soluble protein. Taken together, these data indicate that rice grains expressing hLF can be directly incorporated into infant formula and baby food.

• 대한원격탐사학회지
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• 제24권1호
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• pp.17-24
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• 2008

Rainfall data from three different types of rain gauge system have been collected for the summertime rain event at Mokpo in the Korean peninsula. The rain gauge system considered in this paper is composed of three tipping-bucket rain gauges with 0.1, 0.2, and 0.5 mm measuring resolutions, the Optical Rain Gauge (ORG), and the PARSIVEL (PARticle SIze and VELocity). The PARSIVEL rainfall rate has been considered as the reference for comparison since it gave good resolution and performance on this event. Comparison with the PARSIVEL rainfall rate gives the results that the error and temporal variation of rainfall rate are simultaneously reduced with increasing the averaging interval of rainfall rate or decreasing the size of tipping bucket. This suggests that the estimated rainfall rate must be optimized, differently for the type of tipping-bucket rain gages, by minimizing the averaging interval of rainfall rate under the condition satisfying the given performance of rainfall rate.