• Journal of Microbiology and Biotechnology
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• 제13권2호
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• pp.276-281
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• 2003

The adhesion of probiotic bacteria to the intestinal mucosa is one of the desirable properties for their colonization in the intestinal tract, where these bacteria constantly compete with other bacteria. The adhesion of different strains of bifidobacteria to Caco-2 cells was compared. Among the strains examined, BGN-4 showed the highest adhesion level and the greatest cell surface hydrophobicity (CSH). No close relationship was found between the adhesion and CSH of the strains. Upon protease and heat treatment, the adhesion of the BGN-4 to the Caco-2 cells decreased significantly. The cells grown at $42^{\circ}C$ showed a lower CSH and self-aggregation levels than cells grown at $37^{\circ}C$. The treatment of EGTA did not have any effect on the adhesion. The degree of adhesion did not differ among the experimental groups in which galactose, mannose, or fucose were added in the adhesion assay mixture. The results suggest that the adhesion of the Bifidobacterium to the epithelial cells may be affected by the composition and structure of the cell membrane and interacting surfaces.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2005년도 춘계학술발표논문집
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• pp.277-278
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• 2005

미생물 고정화를 위하여 PU 폼을 세 가지 처리 방법으로 처리하였다. 플라즈마 처리, 플라즈마 유도 그라프팅, 플라즈마 유도 그라프팅 후 플라즈마로 재처리 방법을 사용하였다. 플라즈마 처리는 bacteria adhesion에 큰 효과를 주지 못하였고, 아크릴산의 플라즈마 유도 그라프팅은 adhesion을 세 배 이상 증가시켰다. Bacteria의 adhesion 성능 향상은 표면의 친수성을 증가시키고 새로운 groove나 cavity들을 형성시킴으로 가능하였다. 반면, 그라프팅 후 plasma re-treatment는 미생물 고정화를 크게 향상시키지 못하였다. SEM 관찰을 통하여 대부분의 E.coli.는 groove나 cavities라는 shear-free area에 서식함을 알 수 있었다. PU폼의 bacteria adhesion은 미생물과 고분자 표면 사이의 정전기적 인력이나 van der Waals 인 력에 의해 주로 영향을 받는다고 사료된다.

• 대한치과의사협회지
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• 제50권7호
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• pp.394-406
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• 2012

Objective: The adhesion capabilities of different types of self-ligating brackets were measured with respect to Streptococcus mutans and Streptococcus sobrinus. Methods: Five types of self-ligating brackets (Clippy-C; Mini Clippy; Clarity-SL; Speed; Damon 3) were used for the experiment group and composite resin brackets (Spirit-MB), metal brackets (Victory) and polycrystalline alumina brackets (Clarity) were used for the control group. In order to assess adhesion of bacteria to the brackets, the brackets were cultured for 3, 6 and 24 hours in media containing bacteria and 20% sucrose. Results: There was no statistic difference in adhesion amount of Streptococcus mutans and Streptococcus sobrinus according to the types of brackets. A total adhesion amount according to bracket type was different. An extended incubation time increased adhesion amount. Observation under scanning electron microscope showed that Streptococcus sobrinus adhered more to Clippy-C and Victory rather than to Clarity-SL. Conclusions: Clarity-SL, a self-ligating esthetic bracket was confirmed to show lower bacterial adhesion to cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus group than other self-ligating brackets or conventional brackets, which suggests that proper use of self-ligating esthetic brackets might even be better in preventing tooth surface decalcification.

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.462-464
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• 1989

• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.751-755
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• 2006

Dadih is Indonesian traditional fermented buffalo milk believed by the natives to have beneficial effects on human health. This may be due to the probiotic properties possessed by the lactic acid bacteria (LAB) involved in its fermentation process. It was discovered that ten strains of dadih lactic isolates possessed some probiotic properties in vitro. In this study, the adhesion properties of dadih LAB, in comparison with documented probiotic strains, were investigated in vitro by using mucin extracted from human faeces and Caco-2 cells as the models for human intestinal mucosal surface and intestinal cells respectively. The adhesion results showed the distinction of Lactobacillus reuteri IS-27560 in adhering to both mucus layer and Caco-2 cells. The competition assay for adhesion to the mucus layer between dadih LAB and selected pathogens indicated the competence of Lactococcus lactis IS-16183 and Lactobacillus rhamnosus IS-7257 in significantly inhibiting the adhesion of Escherichia coli O157:H7. Accordingly, these two strains may be potential candidates for use as probiotic strains. Overall, the adhesion properties of all dadih LAB strains were relatively comparable to that of Lactobacillus casei Shirota and Lactobacillus rhamnosus GG, the documented probiotic strains.

• 한국기계가공학회지
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• 제21권5호
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• pp.105-109
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• 2022

In the field of medical and marine industries, antibacterial surfaces have been emerged as one of the most important issues. Recently, many researchers have been studying antibacterial surfaces to kill bacteria or prevent the adhesion of bacteria. In their researches, various materials and structures are suggested to inhibit the adhesion of bacteria or kill the attached bacteria. However, chemical materials such as antibiotics or metal could be toxic. Moreover, frequent use of antibiotics causes super bacteria having resistance to antibiotics. In this study, nano-pillar structured surface was fabricated using polyurethane acrylate (PUA) and the mechanically induced antibacterial function was confirmed based on the fabricated nanostructures. Nanostructures can damage the bacterial membrane of Gram-negative bacteria through stretching of bacterial membrane via interaction with the nanostructures and the bacterial membrane. Consequently, the proposed transparent, flexible and nanostructured PUA films can be one of promising candidates for antifouling and antibacterial surfaces which can be applied in various industries.

• 대한환경공학회지
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• 제29권7호
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• pp.833-838
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• 2007

본 연구에서는 양전하를 띤 여재 입자에서 박테리아의 부착 및 이동에 관한 연구를 위하여 회분 및 칼럼실험을 수행하였다. 본 실험에 사용된 박테리아는 E. coli ATCC 11105(길이: 2.2 ${\mu}m$ 직경: 0.6 ${\mu}m$)이었고, 여재는 석영모래(입경분포: 0.5-2.0 mm, 평균 입경: 1.0 mm)와 철코팅 모래이었다. 회분실험결과에 의하면 철코팅 함량이 증가함에 따라, 박테리아의 부착량이 증가하는 것으로 나타났다. 철코팅 모래의 함량 0%(석영모래 100%)에서는 약 46% 정도의 박테리아가 부착되었고, 철코팅 모래의 함량 100%(석영모래 0%)에서는 약 97% 정도의 박테리아가 부착되었다. 칼럼실험결과 또한 철코팅 함량이 증가함에 따라, 박테리아의 부착량이 증가하는 것을 보여주고 있다. 철코팅 모래의 함량이 0에서 100%로 증가함에 따라 박테리아의 부착량이 8에서 94%로 증가하였다. 본 연구의 실험결과를 통해 양전하를 띤 여재 입자가 박테리아의 이동에 영향을 미칠 수 있음을 알 수 있다.

• 한국콘텐츠학회논문지
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• 제19권2호
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• pp.277-283
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• 2019

본 연구는 치과용시멘트의 표면특성과 구강세균 부착을 위해 치과용시멘트액에 저분자키토산(Low molecular chitosan: LC)을 무게비로 0, 0.5, 1.0, 2.0 wt%를 첨가하였다. 시멘트의 경화시간과 표면특성으로 표면에너지와 표면거칠기를 평가하였다. 구강세균 부착정도는 구강세균 S. mutans, E.coli 2개의 균주를 이용하여 평가하였다. 실험 결과 경화시간은 LC0.5실험군에서 증가하였으나 통계적 유의한 차이는 나타나지 않았다(p<0.05). 표면거칠기는 LC2.0실험군에서 유의한 차이를 보였으며 구강 세균 부착실험 결과 저분자키토산을 첨가한 실험군 모두 통계적으로 유의하게 낮은 부착양상을 보였다(p<0.05). 본 연구로 저분자키토산의 표면특성과 구강세균 부착억제효과가 있음을 입증하였다. 향후 저분자키토산 첨가양에 따른 효율성과 폭 넓은 물성 연구가 필요할 것으로 사료된다.

• 대한치과교정학회지
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• 제52권4호
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• pp.278-286
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• 2022

Objective: To evaluate differences in the adhesion levels of the most common oral pathogens, Streptococcus mutans and Porphyromonas gingivalis, in human saliva-derived microcosm biofilms with respect to time and raw materials of orthodontic brackets. Methods: The samples were classified into three groups of bracket materials: 1) monocrystalline alumina ceramic (CR), 2) stainless steel metal (SS), and 3) polycarbonate plastic (PL), and a hydroxyapatite (HA) group was used to mimic the enamel surface. Saliva was collected from a healthy donor, and saliva-derived biofilms were grown on each sample. A real-time polymerase chain reaction was performed to quantitatively evaluate differences in the attachment levels of total bacteria, S. mutans and P. gingivalis at days 1 and 4. Results: Adhesion of S. mutans and P. gingivalis to CR and HA was higher than the other bracket materials (SS = PL < CR = HA). Total bacteria demonstrated higher adhesion to HA than to bracket materials, but no significant differences in adhesion were observed among the bracket materials (CR = SS = PL < HA). From days 1 to 4, the adhesion of P. gingivalis decreased, while that of S. mutans and total bacteria increased, regardless of material type. Conclusions: The higher adhesion of oral pathogens, such as S. mutans and P. gingivalis to CR suggests that the use of CR brackets possibly facilitates gingival inflammation and enamel decalcification during orthodontic treatment.

• Asian-Australasian Journal of Animal Sciences
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• 제20권9호
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• pp.1397-1401
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• 2007

An in situ experiment was conducted to find out whether Tween 80 improves rice straw digestion through increased adhesion of major fibrolytic bacteria. Rice straw was sprayed with various levels of Tween 80 non-ionic surfactant or SDS ionic surfactant 24 h before incubation in the rumen of Holstein steers. Dry matter (DM) disappearance and adhesion of F. succinogenes, R. flavefaciens and R. albus on rice straw after in situ incubation were measured by real-time PCR. Application of Tween 80 increased DM disappearance, which was more noticeable at an application level of 1% compared to lower application levels. Application of SDS resulted in an opposite response in DM disappearance with highest reduction in DM disappearance at 1% level. In a subsequent in situ experiment, higher Tween 80 was applied to rice straw in an attempt to find the optimum application level. Tween 80 at 2.5% gave better DM disappearance than 1% with a similar result at 5%. Therefore, an adhesion study was carried out using rice straw treated with 2.5% Tween 80. Our results indicated that Tween 80 reduced adhesion of all three major rumen fibrolytic bacteria to rice straw. Present data clearly show that improved DM disappearance by Tween 80 is not due to increased bacterial adhesion onto substrates.